LOCUS       sequence-208471-       14456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vectors used for the inducible expression of tripartite 
            split-sfGFP components in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14456)
  AUTHORS   Liu TY, Chou WC, Chen WY, Chu CY, Dai CY, Wu PY
  TITLE     Detection of membrane protein-protein interaction in planta based on
            dual intein-coupled tripartite split-GFP association.
  JOURNAL   Plant J. 2018 Feb 16. doi: 10.1111/tpj.13874.
  PUBMED    29451720
REFERENCE   2  (bases 1 to 14456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2018 Feb 16. doi: 10.1111/tpj.13874."
COMMENT     Sequence Label: UBQ10:sXVE:(MCS)-S11
FEATURES             Location/Qualifiers
     source          1..14456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(28..831)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGAWIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(139..158)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     749..768
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(881..1060)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4322..4368
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      4557..4809
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4977..5001
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5466..6116
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(6304..6321)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6555..6574)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            6797..6906
                     /note="incP origin of transfer"
     CDS             6939..7310
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      7437..8148
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  8770..9537
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             9729..10523
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             10822..11970
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    13566..13590
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(13837..13858)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14049..14068
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14193..14215
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14207..14224
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14208..14224
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(14237..14437)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
ORIGIN
        1 atccgtcgaa gctagcttgg gtcccgctca gaagaactcg tcaagaaggc gatagaaggc
       61 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
      121 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
      181 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
      241 cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt
      301 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
      361 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
      421 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
      481 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
      541 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
      601 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc
      661 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
      721 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
      781 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc caagctccca tgggccctcg
      841 actagagtcg agatctggat tgagagtgaa tatgagactc taattggata ccgaggggaa
      901 tttatggaac gtcagtggag catttttgac aagaaatatt tgctagctga tagtgacctt
      961 aggcgacttt tgaacgcgca ataatggttt ctgacgtatg tgcttagctc attaaactcc
     1021 agaaacccgc ggctgagtgg ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa
     1081 cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact cccttaattc tccgctcatg
     1141 atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
     1201 agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc
     1261 ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct
     1321 ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc
     1381 accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg
     1441 agtgcttgcg gcagcgtgaa gcttcgacga gtcagtaata aacggcgtca aagtggttgc
     1501 agccggcaca cacgagtcgt gtttatcaac tcaaagcaca aatacttttc ctcaacctaa
     1561 aaataaggca attagccaaa aacaactttg cgtgtaaaca acgctcaata cacgtgtcat
     1621 tttattatta gctattgctt caccgcctta gctttctcgt gacctagtcg tcctcgtctt
     1681 ttcttcttct tcttctataa aacaataccc aaagagctct tcttcttcac aattcagatt
     1741 tcaatttctc aaaatcttaa aaactttctc tcaattctct ctaccgtgat caaggtaaat
     1801 ttctgtgttc cttattctct caaaatcttc gattttgttt tcgttcgatc ccaatttcgt
     1861 atatgttctt tggtttagat tctgttaatc ttagatcgaa gacgattttc tgggtttgat
     1921 cgttagatat catcttaatt ctcgattagg gtttcataga tatcatccga tttgttcaaa
     1981 taatttgagt tttgtcgaat aattactctt cgatttgtga tttctatcta gttctggtgt
     2041 tagtttctag tttgtgcgat cgaatttgtc gattaatctg agtttttctg attaacagac
     2101 tagcccgcgg aaaatgaagg ctcttactgc tagacaacaa gaagtttttg atcttattag
     2161 agatcatatt tctcaaactg gaatgcctcc tactagagct gaaattgctc aaagacttgg
     2221 atttaggtct cctaatgctg ctgaagaaca tcttaaggct cttgctagaa agggagttat
     2281 tgaaattgtt tctggagctt caagaggaat tagacttctt caagaagaag aagaaggact
     2341 tcctcttgtt ggaagagttg ctgctggaga accttcttct gctcctccta ctgatgtttc
     2401 tcttggagat gaacttcatc ttgatggaga agatgttgct atggctcatg ctgatgctct
     2461 tgatgatttt gatcttgata tgcttggaga tggagattct cctggacctg gatttactcc
     2521 tcatgattct gctccttatg gagcacttga tatggctgat tttgaatttg aacaaatgtt
     2581 tactgatgct cttggaattg atgaatatgg aggagatcct tctgctggag atatgagagc
     2641 tgctaatctt tggccttctc ctcttatgat taagaggtct aagaagaact ctcttgctct
     2701 ttctcttact gctgaccaaa tggtttctgc tcttcttgat gctgaacctc ctattcttta
     2761 ttctgaatat gatcctacta gacctttttc tgaggcttct atgatgggac ttcttactaa
     2821 tcttgctgat agagaacttg ttcatatgat taattgggct aagagagttc ctggatttgt
     2881 tgatcttact cttcatgacc aagttcatct tcttgaatgt gcttggcttg aaattcttat
     2941 gattggactt gtttggaggt ctatggaaca tcctgttaaa cttctttttg ctcctaatct
     3001 tcttcttgat agaaatcaag gaaagtgtgt tgaaggaatg gttgaaattt ttgatatgct
     3061 tcttgctact tcttcaagat ttagaatgat gaatcttcaa ggagaagaat ttgtttgtct
     3121 taagtctatt attcttctta attctggagt ttatactttt ctttcttcta ctcttaagtc
     3181 tcttgaagaa aaggatcata ttcatagagt tcttgataag attactgata ctcttattca
     3241 tcttatggct aaggctggac ttactcttca acaacaacat caaagacttg ctcaacttct
     3301 tcttattctt tctcatatta gacatatgtc taataaggga atggaacatc tttattctat
     3361 gaagtgtaag aatgttgttc ctctttatga tcttcttctt gaaatgcttg atgctcatag
     3421 acttcatgct cctacttcaa gaggaggagc ttctgttgaa gaaactgacc aatctcatct
     3481 tgctactgct ggatctactt cttctcattc tcttcaaaag tattatatta ctggagaagc
     3541 tgaaggattt cctgctactg tttgaagatc ttgatcaagc tccctggcca attcccagag
     3601 atgttagctg aaatcatcac taatcagata ccaaaatatt caaatggaaa tatcaaatag
     3661 cttctgtttc atcaaaaatg actcgaccta actgagtaag catgcttgtt cgagtattat
     3721 ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt
     3781 tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga
     3841 tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt
     3901 gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa
     3961 tcgtggcctc taatgaccga agttaatatg aggagtaaaa catcccaaac gcggccgcaa
     4021 ccggtcttgc atccagctgg gctgcaggtc gaggctaaaa aactaatcgc attatcatcc
     4081 cctcgacgta ctgtacatat aaccactggt tttatataca gcagtactgt acatataacc
     4141 actggtttta tatacagcag tcgacgtact gtacatataa ccactggttt tatatacagc
     4201 agtactgtac atataaccac tggttttata tacagcagtc gaggtaagat tagatatgga
     4261 tatgtatatg gatatgtata tggtggtaat gccatgtaat atgctcgact ctaggatctt
     4321 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca cgctgaagct
     4381 agtcgactct agccaaatct aggggatccg gcgcgcccgg gttaattaac tcgagtctag
     4441 tggtggaggg tctggtggcg gatcaaccag tgaaaagcgt gaccacatgg tccttcttga
     4501 gtatgtaact gctgctggga ttacagatgc tagctaaatc gagctcgaat ttccccgatc
     4561 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     4621 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     4681 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     4741 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     4801 tactagatcg gggaattcag atcggctgag tggctccttc aacgttgcgg ttctgtcagt
     4861 tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa
     4921 ttctccgctc atgatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac
     4981 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
     5041 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt
     5101 tccccagatc tggcgccggc cagcgagacg agcaagattg gccgccgccc gaaacgatcc
     5161 gacagcgcgc ccagcacagg tgcgcaggca aattgcacca acgcatacag cgccagcaga
     5221 atgccatagt gggcggtgac gtcgttcgag tgaaccagat cgcgcaggag gcccggcagc
     5281 accggcataa tcaggccgat gccgacagcg tcgagcgcga cagtgctcag aattacgatc
     5341 aggggtatgt tgggtttcac gtctggcctc cggaccagcc tccgctggtc cgattgaacg
     5401 cgcggattct ttatcactga taagttggtg gacatattat gtttatcagt gataaagtgt
     5461 caagcatgac aaagttgcag ccgaatacag tgatccgtgc cgccctggac ctgttgaacg
     5521 aggtcggcgt agacggtctg acgacacgca aactggcgga acggttgggg gttcagcagc
     5581 cggcgcttta ctggcacttc aggaacaagc gggcgctgct cgacgcactg gccgaagcca
     5641 tgctggcgga gaatcatacg cattcggtgc cgagagccga cgacgactgg cgctcatttc
     5701 tgatcgggaa tgcccgcagc ttcaggcagg cgctgctcgc ctaccgcgat ggcgcgcgca
     5761 tccatgccgg cacgcgaccg ggcgcaccgc agatggaaac ggccgacgcg cagcttcgct
     5821 tcctctgcga ggcgggtttt tcggccgggg acgccgtcaa tgcgctgatg acaatcagct
     5881 acttcactgt tggggccgtg cttgaggagc aggccggcga cagcgatgcc ggcgagcgcg
     5941 gcggcaccgt tgaacaggct ccgctctcgc cgctgttgcg ggccgcgata gacgccttcg
     6001 acgaagccgg tccggacgca gcgttcgagc agggactcgc ggtgattgtc gatggattgg
     6061 cgaaaaggag gctcgttgtc aggaacgttg aaggaccgag aaagggtgac gattgatcag
     6121 gaccgctgcc ggagcgcaac ccactcacta cagcagagcc atgtagacaa catcccctcc
     6181 ccctttccac cgcgtcagac gcccgtagca gcccgctacg ggctttttca tgccctgccc
     6241 tagcgtccaa gcctcacggc cgcgctcggc ctctctggcg gccttctggc gctcttccgc
     6301 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6361 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6421 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6481 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6541 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6601 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6661 gcttttccgc tgcataaccc tgcttcgggg tcattatagc gattttttcg gtatatccat
     6721 cctttttcgc acgatataca ggattttgcc aaagggttcg tgtagacttt ccttggtgta
     6781 tccaacggcg tcagccgggc aggataggtg aagtaggccc acccgcgagc gggtgttcct
     6841 tcttcactgt cccttattcg cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct
     6901 ggccggctac cgccggcgta acagatgagg gcaagcggat ggctgatgaa accaagccaa
     6961 ccaggaaggg cagcccacct atcaaggtgt actgccttcc agacgaacga agagcgattg
     7021 aggaaaaggc ggcggcggcc ggcatgagcc tgtcggccta cctgctggcc gtcggccagg
     7081 gctacaaaat cacgggcgtc gtggactatg agcacgtccg cgagctggcc cgcatcaatg
     7141 gcgacctggg ccgcctgggc ggcctgctga aactctggct caccgacgac ccgcgcacgg
     7201 cgcggttcgg tgatgccacg atcctcgccc tgctggcgaa gatcgaagag aagcaggacg
     7261 agcttggcaa ggtcatgatg ggcgtggtcc gcccgagggc agagccatga cttttttagc
     7321 cgctaaaacg gccggggggt gcgcgtgatt gccaagcacg tccccatgcg ctccatcaag
     7381 aagagcgact tcgcggagct ggtgaagtac atcaccgacg agcaaggcaa gaccgagcgc
     7441 ctttccgacg ctcaccgggc tggttgccct cgccgctggg ctggcggccg tctatggccc
     7501 tgcaaacgcg ccagaaacgc cgtcgaagcc gtgtgcgaga caccgcggcc gccggcgttg
     7561 tggatacctc gcggaaaact tggccctcac tgacagatga ggggcggacg ttgacacttg
     7621 aggggccgac tcacccggcg cggcgttgac agatgagggg caggctcgat ttcggccggc
     7681 gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg cgagtttccc
     7741 acagatgatg tggacaagcc tggggataag tgccctgcgg tattgacact tgaggggcgc
     7801 gactactgac agatgagggg cgcgatcctt gacacttgag gggcagagtg ctgacagatg
     7861 aggggcgcac ctattgacat ttgaggggct gtccacaggc agaaaatcca gcatttgcaa
     7921 gggtttccgc ccgtttttcg gccaccgcta acctgtcttt taacctgctt ttaaaccaat
     7981 atttataaac cttgttttta accagggctg cgccctgtgc gcgtgaccgc gcacgccgaa
     8041 ggggggtgcc cccccttctc gaaccctccc ggcccgctaa cgcgggcctc ccatcccccc
     8101 aggggctgcg cccctcggcc gcgaacggcc tcaccccaaa aatggcagcg ctggcagtcc
     8161 ttgccattgc cgggatcggg gcagtaacgg gatgggcgat cagcccgagc gcgacgcccg
     8221 gaagcattga cgtgccgcag gtgctggcat cgacattcag cgaccaggtg ccgggcagtg
     8281 agggcggcgg cctgggtggc ggcctgccct tcacttcggc cgtcggggca ttcacggact
     8341 tcatggcggg gccggcaatt tttaccttgg gcattcttgg catagtggtc gcgggtgccg
     8401 tgctcgtgtt cgggggtgcg ataaacccag cgaaccattt gaggtgatag gtaagattat
     8461 accgaggtat gaaaacgaga attggacctt tacagaatta ctctatgaag cgccatattt
     8521 aaaaagctac caagacgaag aggatgaaga ggatgaggag gcagattgcc ttgaatatat
     8581 tgacaatact gataagataa tatatctttt atatagaaga tatcgccgta tgtaaggatt
     8641 tcagggggca aggcataggc agcgcgctta tcaatatatc tatagaatgg gcaaagcata
     8701 aaaacttgca tggactaatg cttgaaacca ggacaataac cttatagctt gtaaattcta
     8761 tcataattgg gtaatgactc caacttattg atagtgtttt atgttcagat aatgcccgat
     8821 gactttgtca tgcagctcca ccgattttga gaacgacagc gacttccgtc ccagccgtgc
     8881 caggtgctgc ctcagattca ggttatgccg ctcaattcgc tgcgtatatc gcttgctgat
     8941 tacgtgcagc tttcccttca ggcgggattc atacagcggc cagccatccg tcatccatat
     9001 caccacgtca aagggtgaca gcaggctcat aagacgcccc agcgtcgcca tagtgcgttc
     9061 accgaatacg tgcgcaacaa ccgtcttccg gagactgtca tacgcgtaaa acagccagcg
     9121 ctggcgcgat ttagccccga catagcccca ctgttcgtcc atttccgcgc agacgatgac
     9181 gtcactgccc ggctgtatgc gcgaggttac cgactgcggc ctgagttttt taagtgacgt
     9241 aaaatcgtgt tgaggccaac gcccataatg cgggctgttg cccggcatcc aacgccattc
     9301 atggccatat caatgatttt ctggtgcgta ccgggttgag aagcggtgta agtgaactgc
     9361 agttgccatg ttttacggca gtgagagcag agatagcgct gatgtccggc ggtgcttttg
     9421 ccgttacgca ccaccccgtc agtagctgaa caggagggac agctgataga aacagaagcc
     9481 actggagcac ctcaaaaaca ccatcataca ctaaatcagt aagttggcag catcacccat
     9541 aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaacaac
     9601 tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag tgaattggag
     9661 ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa agaggaagga
     9721 aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc
     9781 gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa
     9841 atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg
     9901 aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc
     9961 actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt
    10021 gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt
    10081 gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca
    10141 gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa
    10201 actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg
    10261 aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg
    10321 tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt
    10381 ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa gaacagtatg
    10441 tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata
    10501 ttttactgga tgaattgttt tagtacctag atgtggcgca acgatgccgg cgacaagcag
    10561 gagcgcaccg acttcttccg catcaagtgt tttggctctc aggccgaggc ccacggcaag
    10621 tatttgggca aggggtcgct ggtattcgtg cagggcaaga ttcggaatac caagtacgag
    10681 aaggacggcc agacggtcta cgggaccgac ttcattgccg ataaggtgga ttatctggac
    10741 accaaggcac caggcgggtc aaatcaggaa taagggcaca ttgccccggc gtgagtcggg
    10801 gcaatcccgc aaggagggtg aatgaatcgg acgtttgacc ggaaggcata caggcaagaa
    10861 ctgatcgacg cggggttttc cgccgaggat gccgaaacca tcgcaagccg caccgtcatg
    10921 cgtgcgcccc gcgaaacctt ccagtccgtc ggctcgatgg tccagcaagc tacggccaag
    10981 atcgagcgcg acagcgtgca actggctccc cctgccctgc ccgcgccatc ggccgccgtg
    11041 gagcgttcgc gtcgtctcga acaggaggcg gcaggtttgg cgaagtcgat gaccatcgac
    11101 acgcgaggaa ctatgacgac caagaagcga aaaaccgccg gcgaggacct ggcaaaacag
    11161 gtcagcgagg ccaagcaggc cgcgttgctg aaacacacga agcagcagat caaggaaatg
    11221 cagctttcct tgttcgatat tgcgccgtgg ccggacacga tgcgagcgat gccaaacgac
    11281 acggcccgct ctgccctgtt caccacgcgc aacaagaaaa tcccgcgcga ggcgctgcaa
    11341 aacaaggtca ttttccacgt caacaaggac gtgaagatca cctacaccgg cgtcgagctg
    11401 cgggccgacg atgacgaact ggtgtggcag caggtgttgg agtacgcgaa gcgcacccct
    11461 atcggcgagc cgatcacctt cacgttctac gagctttgcc aggacctggg ctggtcgatc
    11521 aatggccggt attacacgaa ggccgaggaa tgcctgtcgc gcctacaggc gacggcgatg
    11581 ggcttcacgt ccgaccgcgt tgggcacctg gaatcggtgt cgctgctgca ccgcttccgc
    11641 gtcctggacc gtggcaagaa aacgtcccgt tgccaggtcc tgatcgacga ggaaatcgtc
    11701 gtgctgtttg ctggcgacca ctacacgaaa ttcatatggg agaagtaccg caagctgtcg
    11761 ccgacggccc gacggatgtt cgactatttc agctcgcacc gggagccgta cccgctcaag
    11821 ctggaaacct tccgcctcat gtgcggatcg gattccaccc gcgtgaagaa gtggcgcgag
    11881 caggtcggcg aagcctgcga agagttgcga ggcagcggcc tggtggaaca cgcctgggtc
    11941 aatgatgacc tggtgcattg caaacgctag ggccttgtgg ggtcagttcc ggctgggggt
    12001 tcagcagcca gcgctttact ggcatttcag gaacaagcgg gcactgctcg acgcacttgc
    12061 ttcgctcagt atcgctcggg acgcacggcg cgctctacga actgccgata aacagaggat
    12121 taaaattgac aattgtgatt aaggctcaga ttcgacggct tggagcggcc gacgtgcagg
    12181 atttccgcga gatccgattg tcggccctga agaaagctcc agagatgttc gggtccgttt
    12241 acgagcacga ggagaaaaag cccatggagg cgttcgctga acggttgcga gatgccgtgg
    12301 cattcggcgc ctacatcgac ggcgagatca ttgggctgtc ggtcttcaaa caggaggacg
    12361 gccccaagga cgctcacaag gcgcatctgt ccggcgtttt cgtggagccc gaacagcgag
    12421 gccgaggggt cgccggtatg ctgctgcggg cgttgccggc gggtttattg ctcgtgatga
    12481 tcgtccgaca gattccaacg ggaatctggt ggatgcgcat cttcatcctc ggcgcactta
    12541 atatttcgct attctggagc ttgttgttta tttcggtcta ccgcctgccg ggcggggtcg
    12601 cggcgacggt aggcgctgtg cagccgctga tggtcgtgtt catctctgcc gctctgctag
    12661 gtagcccgat acgattgatg gcggtcctgg gggctatttg cggaactgcg ggcgtggcgc
    12721 tgttggtgtt gacaccaaac gcagcgctag atcctgtcgg cgtcgcagcg ggcctggcgg
    12781 gggcggtttc catggcgttc ggaaccgtgc tgacccgcaa gtggcaacct cccgtgcctc
    12841 tgctcacctt taccgcctgg caactggcgg ccggaggact tctgctcgtt ccagtagctt
    12901 tagtgtttga tccgccaatc ccgatgccta caggaaccaa tgttctcggc ctggcgtggc
    12961 tcggcctgat cggagcgggt ttaacctact tcctttggtt ccgggggatc tcgcgactcg
    13021 aacctacagt tgtttcctta ctgggctttc tcagccccag atctggggtc gatcagccgg
    13081 ggatgcatca ggccgacagt cggaacttcg ggtccccgac ctgtaccatt cggtgagcaa
    13141 tggatagggg agttgatatc gtcaacgttc acttctaaag aaatagcgcc actcagcttc
    13201 ctcagcggct ttatccagcg atttcctatt atgtcggcat agttctcaag atcgacagcc
    13261 tgtcacggtt aagcgagaaa tgaataagaa ggctgataat tcggatctct gcgagggaga
    13321 tgatatttga tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg
    13381 cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt
    13441 aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat
    13501 gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg
    13561 gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca
    13621 ttgcggacgt ttttaatgta ctggggtggt ttttcttttc accagtgaga cgggcaacag
    13681 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
    13741 ccccagcagg cgaaaatcct gtttgatggt ggttccgaaa tcggcaaaat cccttataaa
    13801 tcaaaagaat agcccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
    13861 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
    13921 ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
    13981 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
    14041 agaaaggaag ggaagaaagc gaaaggagcg ggcgccattc aggctgcgca actgttggga
    14101 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    14161 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    14221 cagtgaatta attcccatct tgaaagaaat atagtttaaa tatttattga taaaataaca
    14281 agtcaggtat tatagtccaa gcaaaaacat aaatttattg atgcaagttt aaattcagaa
    14341 atatttcaat aactgattat atcagctggt acattgccgt agatgaaaga ctgagtgcga
    14401 tattatgtgt aatacataaa ttgatgatat agctagctta gctcatcggg ggatcg
//
