LOCUS       sequence-208472-       11993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vectors used for the constitutive expression of tripartite 
            split-sfGFP components in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11993)
  AUTHORS   Liu TY, Chou WC, Chen WY, Chu CY, Dai CY, Wu PY
  TITLE     Detection of membrane protein-protein interaction in planta based on
            dual intein-coupled tripartite split-GFP association.
  JOURNAL   Plant J. 2018 Feb 16. doi: 10.1111/tpj.13874.
  PUBMED    29451720
REFERENCE   2  (bases 1 to 11993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2018 Feb 16. doi: 10.1111/tpj.13874."
COMMENT     Sequence Label: P16-Delta-S:S10-(MCS)
FEATURES             Location/Qualifiers
     source          1..11993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(198..215)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(449..468)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            691..800
                     /note="incP origin of transfer"
     CDS             833..1204
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      1331..2042
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  2665..3432
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             3624..4418
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             4717..5865
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    7461..7485
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(7732..7753)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7944..7963
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8088..8110
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8102..8119
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8103..8119
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(8132..8332)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     CDS             complement(8379..9182)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGAWIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8490..8509)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9100..9119
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(9232..9411)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      10445..10697
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10864..10888
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             join(11353..11993,1..10)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
ORIGIN
        1 tgacgattga tcaggaccgc tgccggagcg caacccactc actacagcag agccatgtag
       61 acaacatccc ctcccccttt ccaccgcgtc agacgcccgt agcagcccgc tacgggcttt
      121 ttcatgccct gccctagcgt ccaagcctca cggccgcgct cggcctctct ggcggccttc
      181 tggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      241 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      301 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      361 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      421 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      481 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      541 tcgggaagcg tggcgctttt ccgctgcata accctgcttc ggggtcatta tagcgatttt
      601 ttcggtatat ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga
      661 ctttccttgg tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc
      721 gagcgggtgt tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc
      781 tgctctgcga ggctggccgg ctaccgccgg cgtaacagat gagggcaagc ggatggctga
      841 tgaaaccaag ccaaccagga agggcagccc acctatcaag gtgtactgcc ttccagacga
      901 acgaagagcg attgaggaaa aggcggcggc ggccggcatg agcctgtcgg cctacctgct
      961 ggccgtcggc cagggctaca aaatcacggg cgtcgtggac tatgagcacg tccgcgagct
     1021 ggcccgcatc aatggcgacc tgggccgcct gggcggcctg ctgaaactct ggctcaccga
     1081 cgacccgcgc acggcgcggt tcggtgatgc cacgatcctc gccctgctgg cgaagatcga
     1141 agagaagcag gacgagcttg gcaaggtcat gatgggcgtg gtccgcccga gggcagagcc
     1201 atgacttttt tagccgctaa aacggccggg gggtgcgcgt gattgccaag cacgtcccca
     1261 tgcgctccat caagaagagc gacttcgcgg agctggtgaa gtacatcacc gacgagcaag
     1321 gcaagaccga gcgcctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg
     1381 gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc
     1441 ggccgccggc gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg
     1501 gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct
     1561 cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt
     1621 tacgcgagtt tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga
     1681 cacttgaggg gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag
     1741 agtgctgaca gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa
     1801 tccagcattt gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct
     1861 gcttttaaac caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga
     1921 ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg
     1981 cctcccatcc ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc
     2041 agcgctggca gtccttgcca ttgccgggat cggggcagta acgggatggg cgatcagccc
     2101 gagcgcgacg cccggaagca ttgacgtgcc gcaggtgctg gcatcgacat tcagcgacca
     2161 ggtgccgggc agtgagggcg gcggcctggg tggcggcctg cccttcactt cggccgtcgg
     2221 ggcattcacg gacttcatgg cggggccggc aatttttacc ttgggcattc ttggcatagt
     2281 ggtcgcgggt gccgtgctcg tgttcggggg tgcgataaac ccagcgaacc atttgaggtg
     2341 ataggtaaga ttataccgag gtatgaaaac gagaattgga cctttacaga attactctat
     2401 gaagcgccat atttaaaaag ctaccaagac gaagaggatg aagaggatga ggaggcagat
     2461 tgccttgaat atattgacaa tactgataag ataatatatc ttttatatag aagatatcgc
     2521 cgtatgtaag gatttcaggg ggcaaggcat aggcagcgcg cttatcaata tatctataga
     2581 atgggcaaag cataaaaact tgcatggact aatgcttgaa acccaggaca ataaccttat
     2641 agcttgtaaa ttctatcata attgggtaat gactccaact tattgatagt gttttatgtt
     2701 cagataatgc ccgatgactt tgtcatgcag ctccaccgat tttgagaacg acagcgactt
     2761 ccgtcccagc cgtgccaggt gctgcctcag attcaggtta tgccgctcaa ttcgctgcgt
     2821 atatcgcttg ctgattacgt gcagctttcc cttcaggcgg gattcataca gcggccagcc
     2881 atccgtcatc catatcacca cgtcaaaggg tgacagcagg ctcataagac gccccagcgt
     2941 cgccatagtg cgttcaccga atacgtgcgc aacaaccgtc ttccggagac tgtcatacgc
     3001 gtaaaacagc cagcgctggc gcgatttagc cccgacatag ccccactgtt cgtccatttc
     3061 cgcgcagacg atgacgtcac tgcccggctg tatgcgcgag gttaccgact gcggcctgag
     3121 ttttttaagt gacgtaaaat cgtgttgagg ccaacgccca taatgcgggc tgttgcccgg
     3181 catccaacgc cattcatggc catatcaatg attttctggt gcgtaccggg ttgagaagcg
     3241 gtgtaagtga actgcagttg ccatgtttta cggcagtgag agcagagata gcgctgatgt
     3301 ccggcggtgc ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga gggacagctg
     3361 atagaaacag aagccactgg agcacctcaa aaacaccatc atacactaaa tcagtaagtt
     3421 ggcagcatca cccataattg tggtttcaaa atcggctccg tcgatactat gttatacgcc
     3481 aactttgaaa acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg
     3541 aacagtgaat tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt
     3601 agaaaagagg aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac
     3661 tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata
     3721 agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga
     3781 ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg
     3841 ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg
     3901 ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg
     3961 agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata
     4021 cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac gatctggccg
     4081 atgtggattg cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg
     4141 attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag
     4201 acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg
     4261 gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg
     4321 gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga
     4381 aaataaaata ttatatttta ctggatgaat tgttttagta cctagatgtg gcgcaacgat
     4441 gccggcgaca agcaggagcg caccgacttc ttccgcatca agtgttttgg ctctcaggcc
     4501 gaggcccacg gcaagtattt gggcaagggg tcgctggtat tcgtgcaggg caagattcgg
     4561 aataccaagt acgagaagga cggccagacg gtctacggga ccgacttcat tgccgataag
     4621 gtggattatc tggacaccaa ggcaccaggc gggtcaaatc aggaataagg gcacattgcc
     4681 ccggcgtgag tcggggcaat cccgcaagga gggtgaatga atcggacgtt tgaccggaag
     4741 gcatacaggc aagaactgat cgacgcgggg ttttccgccg aggatgccga aaccatcgca
     4801 agccgcaccg tcatgcgtgc gccccgcgaa accttccagt ccgtcggctc gatggtccag
     4861 caagctacgg ccaagatcga gcgcgacagc gtgcaactgg ctccccctgc cctgcccgcg
     4921 ccatcggccg ccgtggagcg ttcgcgtcgt ctcgaacagg aggcggcagg tttggcgaag
     4981 tcgatgacca tcgacacgcg aggaactatg acgaccaaga agcgaaaaac cgccggcgag
     5041 gacctggcaa aacaggtcag cgaggccaag caggccgcgt tgctgaaaca cacgaagcag
     5101 cagatcaagg aaatgcagct ttccttgttc gatattgcgc cgtggccgga cacgatgcga
     5161 gcgatgccaa acgacacggc ccgctctgcc ctgttcacca cgcgcaacaa gaaaatcccg
     5221 cgcgaggcgc tgcaaaacaa ggtcattttc cacgtcaaca aggacgtgaa gatcacctac
     5281 accggcgtcg agctgcgggc cgacgatgac gaactggtgt ggcagcaggt gttggagtac
     5341 gcgaagcgca cccctatcgg cgagccgatc accttcacgt tctacgagct ttgccaggac
     5401 ctgggctggt cgatcaatgg ccggtattac acgaaggccg aggaatgcct gtcgcgccta
     5461 caggcgacgg cgatgggctt cacgtccgac cgcgttgggc acctggaatc ggtgtcgctg
     5521 ctgcaccgct tccgcgtcct ggaccgtggc aagaaaacgt cccgttgcca ggtcctgatc
     5581 gacgaggaaa tcgtcgtgct gtttgctggc gaccactaca cgaaattcat atgggagaag
     5641 taccgcaagc tgtcgccgac ggcccgacgg atgttcgact atttcagctc gcaccgggag
     5701 ccgtacccgc tcaagctgga aaccttccgc ctcatgtgcg gatcggattc cacccgcgtg
     5761 aagaagtggc gcgagcaggt cggcgaagcc tgcgaagagt tgcgaggcag cggcctggtg
     5821 gaacacgcct gggtcaatga tgacctggtg cattgcaaac gctagggcct tgtggggtca
     5881 gttccggctg ggggttcagc agccagcgct ttactggcat ttcaggaaca agcgggcact
     5941 gctcgacgca cttgcttcgc tcagtatcgc tcgggacgca cggcgcgctc tacgaactgc
     6001 cgataaacag aggattaaaa ttgacaattg tgattaaggc tcagattcga cggcttggag
     6061 cggccgacgt gcaggatttc cgcgagatcc gattgtcggc cctgaagaaa gctccagaga
     6121 tgttcgggtc cgtttacgag cacgaggaga aaaagcccat ggaggcgttc gctgaacggt
     6181 tgcgagatgc cgtggcattc ggcgcctaca tcgacggcga gatcattggg ctgtcggtct
     6241 tcaaacagga ggacggcccc aaggacgctc acaaggcgca tctgtccggc gttttcgtgg
     6301 agcccgaaca gcgaggccga ggggtcgccg gtatgctgct gcgggcgttg ccggcgggtt
     6361 tattgctcgt gatgatcgtc cgacagattc caacgggaat ctggtggatg cgcatcttca
     6421 tcctcggcgc acttaatatt tcgctattct ggagcttgtt gtttatttcg gtctaccgcc
     6481 tgccgggcgg ggtcgcggcg acggtaggcg ctgtgcagcc gctgatggtc gtgttcatct
     6541 ctgccgctct gctaggtagc ccgatacgat tgatggcggt cctgggggct atttgcggaa
     6601 ctgcgggcgt ggcgctgttg gtgttgacac caaacgcagc gctagatcct gtcggcgtcg
     6661 cagcgggcct ggcgggggcg gtttccatgg cgttcggaac cgtgctgacc cgcaagtggc
     6721 aacctcccgt gcctctgctc acctttaccg cctggcaact ggcggccgga ggacttctgc
     6781 tcgttccagt agctttagtg tttgatccgc caatcccgat gcctacagga accaatgttc
     6841 tcggcctggc gtggctcggc ctgatcggag cgggtttaac ctacttcctt tggttccggg
     6901 ggatctcgcg actcgaacct acagttgttt ccttactggg ctttctcagc cccagatctg
     6961 gggtcgatca gccggggatg catcaggccg acagtcggaa cttcgggtcc ccgacctgta
     7021 ccattcggtg agcaatggat aggggagttg atatcgtcaa cgttcacttc taaagaaata
     7081 gcgccactca gcttcctcag cggctttatc cagcgatttc ctattatgtc ggcatagttc
     7141 tcaagatcga cagcctgtca cggttaagcg agaaatgaat aagaaggctg ataattcgga
     7201 tctctgcgag ggagatgata tttgatcaca ggcagcaacg ctctgtcatc gttacaatca
     7261 acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt
     7321 ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg
     7381 ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
     7441 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga
     7501 caacttaata acacattgcg gacgttttta atgtactggg gtggtttttc ttttcaccag
     7561 tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg
     7621 gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggttc cgaaatcggc
     7681 aaaatccctt ataaatcaaa agaatagccc gagatagggt tgagtgttgt tccagtttgg
     7741 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     7801 cagggcgatg gcccactacg tgaaccatca cccaaatcaa gttttttggg gtcgaggtgc
     7861 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     7921 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc cattcaggct
     7981 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     8041 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     8101 ttgtaaaacg acggccagtg aattaattcc catcttgaaa gaaatatagt ttaaatattt
     8161 attgataaaa taacaagtca ggtattatag tccaagcaaa aacataaatt tattgatgca
     8221 agtttaaatt cagaaatatt tcaataactg attatatcag ctggtacatt gccgtagatg
     8281 aaagactgag tgcgatatta tgtgtaatac ataaattgat gatatagcta gcttagctca
     8341 tcgggggatc gatccgtcga agctagcttg ggtcccgctc agaagaactc gtcaagaagg
     8401 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     8461 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     8521 tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     8581 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     8641 atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     8701 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     8761 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     8821 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     8881 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     8941 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctgcagttca
     9001 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     9061 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     9121 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat ccaagctccc
     9181 atgggccctc gactagagtc gagatctgga ttgagagtga atatgagact ctaattggat
     9241 accgagggga atttatggaa cgtcagtgga gcatttttga caagaaatat ttgctagctg
     9301 atagtgacct taggcgactt ttgaacgcgc aataatggtt tctgacgtat gtgcttagct
     9361 cattaaactc cagaaacccg cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc
     9421 caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt
     9481 ctccgctcat gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca
     9541 gtttactttg cagggcttcc caaccttacc agagggcgcc ccagctggca attccggttc
     9601 gcttgctgtc cataaaaccg cccagtctag ctatcgccat gtaagcccac tgcaagctac
     9661 ctgctttctc tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat
     9721 ccggggtcag caccgtttct gcggactggc tttctacgtg ttccgcttcc tttagcagcc
     9781 cttgcgccct gagtgcttgc ggcagcgtga agctttggaa ccatcttttg ggttcctttc
     9841 ttcttctctt tttttgtttc tctcttaaaa gtcttttctc ttttcaagtg cttcaaacaa
     9901 aactaatttg ttataaaggg agtttctcta ttttattata tagcaaaaaa cttccaaaat
     9961 ttctcatttc tgtgtttaac cttttcgtac atcagtttta aagcacagag tgctcaatgt
    10021 tcttccaata tcgttattaa taaattttga ttaaattcaa tcaaatcgga gttatattac
    10081 cacatgaggt taaagggcca tattaaaaag tctgcacttc atatgagcaa caaggctttt
    10141 atgtctttat ggttgatttg atggcccata tatgatagtt caaaggccca tattaaaaaa
    10201 tgccctaaca tgattcgatt cgtatataaa gacgcttaat gtgacaagcg aacttgctca
    10261 ttagggtttc tcatctacga cggcgtgggc tagaggatcc atggatttac cagacgacca
    10321 ttacctgtca acacaaacta tcctttcgaa agatctcaac ggtacggacg ttgggtctgg
    10381 cggtggctcc ggcgcgcccg ggttaattaa ctcgagtcta gctaaatcga gctcgaattt
    10441 ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
    10501 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
    10561 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
    10621 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
    10681 atctatgtta ctagatcggg aattcagatc ggctgagtgg ctccttcaac gttgcggttc
    10741 tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact
    10801 cccttaattc tccgctcatg atcagattgt cgtttcccgc cttcagttta aactatcagt
    10861 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
    10921 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
    10981 acagggttcc ccagatctgg cgccggccag cgagacgagc aagattggcc gccgcccgaa
    11041 acgatccgac agcgcgccca gcacaggtgc gcaggcaaat tgcaccaacg catacagcgc
    11101 cagcagaatg ccatagtggg cggtgacgtc gttcgagtga accagatcgc gcaggaggcc
    11161 cggcagcacc ggcataatca ggccgatgcc gacagcgtcg agcgcgacag tgctcagaat
    11221 tacgatcagg ggtatgttgg gtttcacgtc tggcctccgg accagcctcc gctggtccga
    11281 ttgaacgcgc ggattcttta tcactgataa gttggtggac atattatgtt tatcagtgat
    11341 aaagtgtcaa gcatgacaaa gttgcagccg aatacagtga tccgtgccgc cctggacctg
    11401 ttgaacgagg tcggcgtaga cggtctgacg acacgcaaac tggcggaacg gttgggggtt
    11461 cagcagccgg cgctttactg gcacttcagg aacaagcggg cgctgctcga cgcactggcc
    11521 gaagccatgc tggcggagaa tcatacgcat tcggtgccga gagccgacga cgactggcgc
    11581 tcatttctga tcgggaatgc ccgcagcttc aggcaggcgc tgctcgccta ccgcgatggc
    11641 gcgcgcatcc atgccggcac gcgaccgggc gcaccgcaga tggaaacggc cgacgcgcag
    11701 cttcgcttcc tctgcgaggc gggtttttcg gccggggacg ccgtcaatgc gctgatgaca
    11761 atcagctact tcactgttgg ggccgtgctt gaggagcagg ccggcgacag cgatgccggc
    11821 gagcgcggcg gcaccgttga acaggctccg ctctcgccgc tgttgcgggc cgcgatagac
    11881 gccttcgacg aagccggtcc ggacgcagcg ttcgagcagg gactcgcggt gattgtcgat
    11941 ggattggcga aaaggaggct cgttgtcagg aacgttgaag gaccgagaaa ggg
//
