LOCUS       sequence-208478-       14943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vectors used for the inducible expression of tripartite 
            split-sfGFP components (GFP1-9) in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14943)
  AUTHORS   Liu TY, Chou WC, Chen WY, Chu CY, Dai CY, Wu PY
  TITLE     Detection of membrane protein-protein interaction in planta based on
            dual intein-coupled tripartite split-GFP association.
  JOURNAL   Plant J. 2018 Feb 16. doi: 10.1111/tpj.13874.
  PUBMED    29451720
REFERENCE   2  (bases 1 to 14943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2018 Feb 16. doi: 10.1111/tpj.13874."
COMMENT     Sequence Label: UBQ10:sXVE:GFP1–9
FEATURES             Location/Qualifiers
     source          1..14943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  366..1133
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             1325..2119
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             2418..3566
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    5162..5186
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(5433..5454)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5645..5664
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5789..5811
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5803..5820
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5804..5820
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(5833..6033)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     CDS             complement(6080..6883)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGAWIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6191..6210)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6801..6820
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6933..7112)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        10374..10420
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      11096..11348
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    11515..11539
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12004..12654
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(12842..12859)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(13093..13112)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            13335..13444
                     /note="incP origin of transfer"
     CDS             13477..13848
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      13975..14686
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 gtgttcgggg gtgcgataaa cccagcgaac catttgaggt gataggtaag attataccga
       61 ggtatgaaaa cgagaattgg acctttacag aattactcta tgaagcgcca tatttaaaaa
      121 gctaccaaga cgaagaggat gaagaggatg aggaggcaga ttgccttgaa tatattgaca
      181 atactgataa gataatatat cttttatata gaagatatcg ccgtatgtaa ggatttcagg
      241 gggcaaggca taggcagcgc gcttatcaat atatctatag aatgggcaaa gcataaaaac
      301 ttgcatggac taatgcttga aacccaggac aataacctta tagcttgtaa attctatcat
      361 aattgggtaa tgactccaac ttattgatag tgttttatgt tcagataatg cccgatgact
      421 ttgtcatgca gctccaccga ttttgagaac gacagcgact tccgtcccag ccgtgccagg
      481 tgctgcctca gattcaggtt atgccgctca attcgctgcg tatatcgctt gctgattacg
      541 tgcagctttc ccttcaggcg ggattcatac agcggccagc catccgtcat ccatatcacc
      601 acgtcaaagg gtgacagcag gctcataaga cgccccagcg tcgccatagt gcgttcaccg
      661 aatacgtgcg caacaaccgt cttccggaga ctgtcatacg cgtaaaacag ccagcgctgg
      721 cgcgatttag ccccgacata gccccactgt tcgtccattt ccgcgcagac gatgacgtca
      781 ctgcccggct gtatgcgcga ggttaccgac tgcggcctga gttttttaag tgacgtaaaa
      841 tcgtgttgag gccaacgccc ataatgcggg ctgttgcccg gcatccaacg ccattcatgg
      901 ccatatcaat gattttctgg tgcgtaccgg gttgagaagc ggtgtaagtg aactgcagtt
      961 gccatgtttt acggcagtga gagcagagat agcgctgatg tccggcggtg cttttgccgt
     1021 tacgcaccac cccgtcagta gctgaacagg agggacagct gatagaaaca gaagccactg
     1081 gagcacctca aaaacaccat catacactaa atcagtaagt tggcagcatc acccataatt
     1141 gtggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
     1201 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
     1261 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
     1321 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
     1381 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
     1441 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
     1501 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
     1561 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
     1621 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
     1681 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
     1741 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
     1801 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
     1861 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     1921 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     1981 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     2041 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     2101 actggatgaa ttgttttagt acctagatgt ggcgcaacga tgccggcgac aagcaggagc
     2161 gcaccgactt cttccgcatc aagtgttttg gctctcaggc cgaggcccac ggcaagtatt
     2221 tgggcaaggg gtcgctggta ttcgtgcagg gcaagattcg gaataccaag tacgagaagg
     2281 acggccagac ggtctacggg accgacttca ttgccgataa ggtggattat ctggacacca
     2341 aggcaccagg cgggtcaaat caggaataag ggcacattgc cccggcgtga gtcggggcaa
     2401 tcccgcaagg agggtgaatg aatcggacgt ttgaccggaa ggcatacagg caagaactga
     2461 tcgacgcggg gttttccgcc gaggatgccg aaaccatcgc aagccgcacc gtcatgcgtg
     2521 cgccccgcga aaccttccag tccgtcggct cgatggtcca gcaagctacg gccaagatcg
     2581 agcgcgacag cgtgcaactg gctccccctg ccctgcccgc gccatcggcc gccgtggagc
     2641 gttcgcgtcg tctcgaacag gaggcggcag gtttggcgaa gtcgatgacc atcgacacgc
     2701 gaggaactat gacgaccaag aagcgaaaaa ccgccggcga ggacctggca aaacaggtca
     2761 gcgaggccaa gcaggccgcg ttgctgaaac acacgaagca gcagatcaag gaaatgcagc
     2821 tttccttgtt cgatattgcg ccgtggccgg acacgatgcg agcgatgcca aacgacacgg
     2881 cccgctctgc cctgttcacc acgcgcaaca agaaaatccc gcgcgaggcg ctgcaaaaca
     2941 aggtcatttt ccacgtcaac aaggacgtga agatcaccta caccggcgtc gagctgcggg
     3001 ccgacgatga cgaactggtg tggcagcagg tgttggagta cgcgaagcgc acccctatcg
     3061 gcgagccgat caccttcacg ttctacgagc tttgccagga cctgggctgg tcgatcaatg
     3121 gccggtatta cacgaaggcc gaggaatgcc tgtcgcgcct acaggcgacg gcgatgggct
     3181 tcacgtccga ccgcgttggg cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc
     3241 tggaccgtgg caagaaaacg tcccgttgcc aggtcctgat cgacgaggaa atcgtcgtgc
     3301 tgtttgctgg cgaccactac acgaaattca tatgggagaa gtaccgcaag ctgtcgccga
     3361 cggcccgacg gatgttcgac tatttcagct cgcaccggga gccgtacccg ctcaagctgg
     3421 aaaccttccg cctcatgtgc ggatcggatt ccacccgcgt gaagaagtgg cgcgagcagg
     3481 tcggcgaagc ctgcgaagag ttgcgaggca gcggcctggt ggaacacgcc tgggtcaatg
     3541 atgacctggt gcattgcaaa cgctagggcc ttgtggggtc agttccggct gggggttcag
     3601 cagccagcgc tttactggca tttcaggaac aagcgggcac tgctcgacgc acttgcttcg
     3661 ctcagtatcg ctcgggacgc acggcgcgct ctacgaactg ccgataaaca gaggattaaa
     3721 attgacaatt gtgattaagg ctcagattcg acggcttgga gcggccgacg tgcaggattt
     3781 ccgcgagatc cgattgtcgg ccctgaagaa agctccagag atgttcgggt ccgtttacga
     3841 gcacgaggag aaaaagccca tggaggcgtt cgctgaacgg ttgcgagatg ccgtggcatt
     3901 cggcgcctac atcgacggcg agatcattgg gctgtcggtc ttcaaacagg aggacggccc
     3961 caaggacgct cacaaggcgc atctgtccgg cgttttcgtg gagcccgaac agcgaggccg
     4021 aggggtcgcc ggtatgctgc tgcgggcgtt gccggcgggt ttattgctcg tgatgatcgt
     4081 ccgacagatt ccaacgggaa tctggtggat gcgcatcttc atcctcggcg cacttaatat
     4141 ttcgctattc tggagcttgt tgtttatttc ggtctaccgc ctgccgggcg gggtcgcggc
     4201 gacggtaggc gctgtgcagc cgctgatggt cgtgttcatc tctgccgctc tgctaggtag
     4261 cccgatacga ttgatggcgg tcctgggggc tatttgcgga actgcgggcg tggcgctgtt
     4321 ggtgttgaca ccaaacgcag cgctagatcc tgtcggcgtc gcagcgggcc tggcgggggc
     4381 ggtttccatg gcgttcggaa ccgtgctgac ccgcaagtgg caacctcccg tgcctctgct
     4441 cacctttacc gcctggcaac tggcggccgg aggacttctg ctcgttccag tagctttagt
     4501 gtttgatccg ccaatcccga tgcctacagg aaccaatgtt ctcggcctgg cgtggctcgg
     4561 cctgatcgga gcgggtttaa cctacttcct ttggttccgg gggatctcgc gactcgaacc
     4621 tacagttgtt tccttactgg gctttctcag ccccagatct ggggtcgatc agccggggat
     4681 gcatcaggcc gacagtcgga acttcgggtc cccgacctgt accattcggt gagcaatgga
     4741 taggggagtt gatatcgtca acgttcactt ctaaagaaat agcgccactc agcttcctca
     4801 gcggctttat ccagcgattt cctattatgt cggcatagtt ctcaagatcg acagcctgtc
     4861 acggttaagc gagaaatgaa taagaaggct gataattcgg atctctgcga gggagatgat
     4921 atttgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag
     4981 atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca
     5041 tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc
     5101 tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg
     5161 gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc
     5221 ggacgttttt aatgtactgg ggtggttttt cttttcacca gtgagacggg caacagctga
     5281 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc
     5341 agcaggcgaa aatcctgttt gatggtggtt ccgaaatcgg caaaatccct tataaatcaa
     5401 aagaatagcc cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     5461 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     5521 gtgaaccatc acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     5581 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     5641 aggaagggaa gaaagcgaaa ggagcgggcg ccattcaggc tgcgcaactg ttgggaaggg
     5701 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     5761 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     5821 gaattaattc ccatcttgaa agaaatatag tttaaatatt tattgataaa ataacaagtc
     5881 aggtattata gtccaagcaa aaacataaat ttattgatgc aagtttaaat tcagaaatat
     5941 ttcaataact gattatatca gctggtacat tgccgtagat gaaagactga gtgcgatatt
     6001 atgtgtaata cataaattga tgatatagct agcttagctc atcgggggat cgatccgtcg
     6061 aagctagctt gggtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct
     6121 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
     6181 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
     6241 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
     6301 aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg
     6361 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
     6421 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg
     6481 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
     6541 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
     6601 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
     6661 tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt
     6721 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
     6781 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
     6841 gagaacctgc gtgcaatcca tcttgttcaa tccaagctcc catgggccct cgactagagt
     6901 cgagatctgg attgagagtg aatatgagac tctaattgga taccgagggg aatttatgga
     6961 acgtcagtgg agcatttttg acaagaaata tttgctagct gatagtgacc ttaggcgact
     7021 tttgaacgcg caataatggt ttctgacgta tgtgcttagc tcattaaact ccagaaaccc
     7081 gcggctgagt ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt
     7141 cccgcgtcat cggcgggggt cataacgtga ctcccttaat tctccgctca tgatcttgat
     7201 cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc
     7261 ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc
     7321 gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt
     7381 gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc
     7441 tgcggactgg ctttctacgt gttccgcttc ctttagcagc ccttgcgccc tgagtgcttg
     7501 cggcagcgtg aagcttcgac gagtcagtaa taaacggcgt caaagtggtt gcagccggca
     7561 cacacgagtc gtgtttatca actcaaagca caaatacttt tcctcaacct aaaaataagg
     7621 caattagcca aaaacaactt tgcgtgtaaa caacgctcaa tacacgtgtc attttattat
     7681 tagctattgc ttcaccgcct tagctttctc gtgacctagt cgtcctcgtc ttttcttctt
     7741 cttcttctat aaaacaatac ccaaagagct cttcttcttc acaattcaga tttcaatttc
     7801 tcaaaatctt aaaaactttc tctcaattct ctctaccgtg atcaaggtaa atttctgtgt
     7861 tccttattct ctcaaaatct tcgattttgt tttcgttcga tcccaatttc gtatatgttc
     7921 tttggtttag attctgttaa tcttagatcg aagacgattt tctgggtttg atcgttagat
     7981 atcatcttaa ttctcgatta gggtttcata gatatcatcc gatttgttca aataatttga
     8041 gttttgtcga ataattactc ttcgatttgt gatttctatc tagttctggt gttagtttct
     8101 agtttgtgcg atcgaatttg tcgattaatc tgagtttttc tgattaacag actagcccgc
     8161 ggaaaatgaa ggctcttact gctagacaac aagaagtttt tgatcttatt agagatcata
     8221 tttctcaaac tggaatgcct cctactagag ctgaaattgc tcaaagactt ggatttaggt
     8281 ctcctaatgc tgctgaagaa catcttaagg ctcttgctag aaagggagtt attgaaattg
     8341 tttctggagc ttcaagagga attagacttc ttcaagaaga agaagaagga cttcctcttg
     8401 ttggaagagt tgctgctgga gaaccttctt ctgctcctcc tactgatgtt tctcttggag
     8461 atgaacttca tcttgatgga gaagatgttg ctatggctca tgctgatgct cttgatgatt
     8521 ttgatcttga tatgcttgga gatggagatt ctcctggacc tggatttact cctcatgatt
     8581 ctgctcctta tggagcactt gatatggctg attttgaatt tgaacaaatg tttactgatg
     8641 ctcttggaat tgatgaatat ggaggagatc cttctgctgg agatatgaga gctgctaatc
     8701 tttggccttc tcctcttatg attaagaggt ctaagaagaa ctctcttgct ctttctctta
     8761 ctgctgacca aatggtttct gctcttcttg atgctgaacc tcctattctt tattctgaat
     8821 atgatcctac tagacctttt tctgaggctt ctatgatggg acttcttact aatcttgctg
     8881 atagagaact tgttcatatg attaattggg ctaagagagt tcctggattt gttgatctta
     8941 ctcttcatga ccaagttcat cttcttgaat gtgcttggct tgaaattctt atgattggac
     9001 ttgtttggag gtctatggaa catcctgtta aacttctttt tgctcctaat cttcttcttg
     9061 atagaaatca aggaaagtgt gttgaaggaa tggttgaaat ttttgatatg cttcttgcta
     9121 cttcttcaag atttagaatg atgaatcttc aaggagaaga atttgtttgt cttaagtcta
     9181 ttattcttct taattctgga gtttatactt ttctttcttc tactcttaag tctcttgaag
     9241 aaaaggatca tattcataga gttcttgata agattactga tactcttatt catcttatgg
     9301 ctaaggctgg acttactctt caacaacaac atcaaagact tgctcaactt cttcttattc
     9361 tttctcatat tagacatatg tctaataagg gaatggaaca tctttattct atgaagtgta
     9421 agaatgttgt tcctctttat gatcttcttc ttgaaatgct tgatgctcat agacttcatg
     9481 ctcctacttc aagaggagga gcttctgttg aagaaactga ccaatctcat cttgctactg
     9541 ctggatctac ttcttctcat tctcttcaaa agtattatat tactggagaa gctgaaggat
     9601 ttcctgctac tgtttgaaga tcttgatcaa gctccctggc caattcccag agatgttagc
     9661 tgaaatcatc actaatcaga taccaaaata ttcaaatgga aatatcaaat agcttctgtt
     9721 tcatcaaaaa tgactcgacc taactgagta agcatgcttg ttcgagtatt atggcattgg
     9781 gaaaactgtt tttcttgtac catttgttgt gcttgtaatt tactgtgttt tttattcggt
     9841 tttcgctatc gaactgtgaa atggaaatgg atggagaaga gttaatgaat gatatggtcc
     9901 ttttgttcat tctcaaatta atattatttg ttttttctct tatttgttgt gtgttgaatt
     9961 tgaaattata agagatatgc aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc
    10021 tctaatgacc gaagttaata tgaggagtaa aacatcccaa acgcggccgc aaccggtctt
    10081 gcatccagct gggctgcagg tcgaggctaa aaaactaatc gcattatcat cccctcgacg
    10141 tactgtacat ataaccactg gttttatata cagcagtact gtacatataa ccactggttt
    10201 tatatacagc agtcgacgta ctgtacatat aaccactggt tttatataca gcagtactgt
    10261 acatataacc actggtttta tatacagcag tcgaggtaag attagatatg gatatgtata
    10321 tggatatgta tatggtggta atgccatgta atatgctcga ctctaggatc ttcgcaagac
    10381 ccttcctcta tataaggaag ttcatttcat ttggagagga cacgctgaag ctagtcgact
    10441 ctagccaaat ctagagttaa ccgggctcag gcctggcgcg ccactagtat gagaaaagga
    10501 gaagaacttt tcactggagt tgtcccaatt cttattgaat tagatggtga tgttaatggg
    10561 cacaaatttt ttgtccgtgg agagggtgaa ggtgatgcaa caatcggaaa acttagcctt
    10621 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactaccctt
    10681 acctatggtg ttcaatgctt ttcaagatac ccagatcata tgaagcggca cgacttcttc
    10741 aagagcgcca tgcctgaggg atacgtgcag gagaggacca tctatttcaa ggacgacggg
    10801 acttacaaga cacgtgctga agtcaagttt gagggagaca ccctcgtcaa caggatcgag
    10861 cttaagggaa ttgatttcaa ggaggacgga aacatcctcg gccacaagtt ggaatacaac
    10921 tttaactccc acaaagtata catcacggcc gacaagcaaa ataacggcat caaagccaac
    10981 ttcacaattc gccacaacgt tgaagacggc tctgtgcaac tcgctgatca ttatcaacaa
    11041 aatactccaa ttggcgatgg ccctgtcctt ttaccataag agctcgaatt tccccgatcg
    11101 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
    11161 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
    11221 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
    11281 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
    11341 actagatcgg gaattcagat cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc
    11401 caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt
    11461 ctccgctcat gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag
    11521 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
    11581 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
    11641 cccagatctg gcgccggcca gcgagacgag caagattggc cgccgcccga aacgatccga
    11701 cagcgcgccc agcacaggtg cgcaggcaaa ttgcaccaac gcatacagcg ccagcagaat
    11761 gccatagtgg gcggtgacgt cgttcgagtg aaccagatcg cgcaggaggc ccggcagcac
    11821 cggcataatc aggccgatgc cgacagcgtc gagcgcgaca gtgctcagaa ttacgatcag
    11881 gggtatgttg ggtttcacgt ctggcctccg gaccagcctc cgctggtccg attgaacgcg
    11941 cggattcttt atcactgata agttggtgga catattatgt ttatcagtga taaagtgtca
    12001 agcatgacaa agttgcagcc gaatacagtg atccgtgccg ccctggacct gttgaacgag
    12061 gtcggcgtag acggtctgac gacacgcaaa ctggcggaac ggttgggggt tcagcagccg
    12121 gcgctttact ggcacttcag gaacaagcgg gcgctgctcg acgcactggc cgaagccatg
    12181 ctggcggaga atcatacgca ttcggtgccg agagccgacg acgactggcg ctcatttctg
    12241 atcgggaatg cccgcagctt caggcaggcg ctgctcgcct accgcgatgg cgcgcgcatc
    12301 catgccggca cgcgaccggg cgcaccgcag atggaaacgg ccgacgcgca gcttcgcttc
    12361 ctctgcgagg cgggtttttc ggccggggac gccgtcaatg cgctgatgac aatcagctac
    12421 ttcactgttg gggccgtgct tgaggagcag gccggcgaca gcgatgccgg cgagcgcggc
    12481 ggcaccgttg aacaggctcc gctctcgccg ctgttgcggg ccgcgataga cgccttcgac
    12541 gaagccggtc cggacgcagc gttcgagcag ggactcgcgg tgattgtcga tggattggcg
    12601 aaaaggaggc tcgttgtcag gaacgttgaa ggaccgagaa agggtgacga ttgatcagga
    12661 ccgctgccgg agcgcaaccc actcactaca gcagagccat gtagacaaca tcccctcccc
    12721 ctttccaccg cgtcagacgc ccgtagcagc ccgctacggg ctttttcatg ccctgcccta
    12781 gcgtccaagc ctcacggccg cgctcggcct ctctggcggc cttctggcgc tcttccgctt
    12841 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    12901 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    12961 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    13021 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    13081 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    13141 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    13201 ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc
    13261 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc
    13321 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc
    13381 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg
    13441 ccggctaccg ccggcgtaac agatgagggc aagcggatgg ctgatgaaac caagccaacc
    13501 aggaagggca gcccacctat caaggtgtac tgccttccag acgaacgaag agcgattgag
    13561 gaaaaggcgg cggcggccgg catgagcctg tcggcctacc tgctggccgt cggccagggc
    13621 tacaaaatca cgggcgtcgt ggactatgag cacgtccgcg agctggcccg catcaatggc
    13681 gacctgggcc gcctgggcgg cctgctgaaa ctctggctca ccgacgaccc gcgcacggcg
    13741 cggttcggtg atgccacgat cctcgccctg ctggcgaaga tcgaagagaa gcaggacgag
    13801 cttggcaagg tcatgatggg cgtggtccgc ccgagggcag agccatgact tttttagccg
    13861 ctaaaacggc cggggggtgc gcgtgattgc caagcacgtc cccatgcgct ccatcaagaa
    13921 gagcgacttc gcggagctgg tgaagtacat caccgacgag caaggcaaga ccgagcgcct
    13981 ttccgacgct caccgggctg gttgccctcg ccgctgggct ggcggccgtc tatggccctg
    14041 caaacgcgcc agaaacgccg tcgaagccgt gtgcgagaca ccgcggccgc cggcgttgtg
    14101 gatacctcgc ggaaaacttg gccctcactg acagatgagg ggcggacgtt gacacttgag
    14161 gggccgactc acccggcgcg gcgttgacag atgaggggca ggctcgattt cggccggcga
    14221 cgtggagctg gccagcctcg caaatcggcg aaaacgcctg attttacgcg agtttcccac
    14281 agatgatgtg gacaagcctg gggataagtg ccctgcggta ttgacacttg aggggcgcga
    14341 ctactgacag atgaggggcg cgatccttga cacttgaggg gcagagtgct gacagatgag
    14401 gggcgcacct attgacattt gaggggctgt ccacaggcag aaaatccagc atttgcaagg
    14461 gtttccgccc gtttttcggc caccgctaac ctgtctttta acctgctttt aaaccaatat
    14521 ttataaacct tgtttttaac cagggctgcg ccctgtgcgc gtgaccgcgc acgccgaagg
    14581 ggggtgcccc cccttctcga accctcccgg cccgctaacg cgggcctccc atccccccag
    14641 gggctgcgcc cctcggccgc gaacggcctc accccaaaaa tggcagcgct ggcagtcctt
    14701 gccattgccg ggatcggggc agtaacggga tgggcgatca gcccgagcgc gacgcccgga
    14761 agcattgacg tgccgcaggt gctggcatcg acattcagcg accaggtgcc gggcagtgag
    14821 ggcggcggcc tgggtggcgg cctgcccttc acttcggccg tcggggcatt cacggacttc
    14881 atggcggggc cggcaatttt taccttgggc attcttggca tagtggtcgc gggtgccgtg
    14941 ctc
//
