LOCUS       sequence-208479-       14561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vectors used for the inducible expression of tripartite 
            split-sfGFP components (3xHA-S11 as control) in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14561)
  AUTHORS   Liu TY, Chou WC, Chen WY, Chu CY, Dai CY, Wu PY
  TITLE     Detection of membrane protein-protein interaction in planta based on
            dual intein-coupled tripartite split-GFP association.
  JOURNAL   Plant J. 2018 Feb 16. doi: 10.1111/tpj.13874.
  PUBMED    29451720
REFERENCE   2  (bases 1 to 14561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2018 Feb 16. doi: 10.1111/tpj.13874."
COMMENT     Sequence Label: UBQ10:sXVE:ATG-3xHA-S11
FEATURES             Location/Qualifiers
     source          1..14561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        154..200
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             283..372
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      494..746
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             1403..2053
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(2241..2258)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2492..2511)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            2734..2843
                     /note="incP origin of transfer"
     CDS             2876..3247
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      3374..4085
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  4707..5474
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             5666..6460
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             6759..7907
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    9503..9527
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(9774..9795)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9986..10005
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10130..10152
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10144..10161
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10145..10161
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10174..10374)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     CDS             complement(10421..11224)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGAWIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10532..10551)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     11142..11161
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(11274..11453)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 ctgtacatat aaccactggt tttatataca gcagtactgt acatataacc actggtttta
       61 tatacagcag tcgaggtaag attagatatg gatatgtata tggatatgta tatggtggta
      121 atgccatgta atatgctcga ctctaggatc ttcgcaagac ccttcctcta tataaggaag
      181 ttcatttcat ttggagagga cacgctgaag ctagtcgact ctagccaaat ctaggggatc
      241 cggcgcgccc gggttaatta actcgagtct agaatgtcga cttacccata cgatgttcca
      301 gattacgctg gctacccata cgatgttcca gattacgctg gatcataccc atacgatgtt
      361 ccagattacg ctactagtgg tggagggtct ggtggcggat caaccagtga aaagcgtgac
      421 cacatggtcc ttcttgagta tgtaactgct gctgggatta cagatgctag ctaaatcgag
      481 ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
      541 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
      601 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
      661 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
      721 cgcggtgtca tctatgttac tagatcgggg aattcagatc ggctgagtgg ctccttcaac
      781 gttgcggttc tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca
      841 taacgtgact cccttaattc tccgctcatg atcagattgt cgtttcccgc cttcagttta
      901 aactatcagt gtttgacagg atatattskc nnntanncct aagagaaaag agcgtttatt
      961 agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg
     1021 catgccaacc acagggttcc ccagatctgg cgccggccag cgagacgagc aagattggcc
     1081 gccgcccgaa acgatccgac agcgcgccca gcacaggtgc gcaggcaaat tgcaccaacg
     1141 catacagcgc cagcagaatg ccatagtggg cggtgacgtc gttcgagtga accagatcgc
     1201 gcaggaggcc cggcagcacc ggcataatca ggccgatgcc gacagcgtcg agcgcgacag
     1261 tgctcagaat tacgatcagg ggtatgttgg gtttcacgtc tggcctccgg accagcctcc
     1321 gctggtccga ttgaacgcgc ggattcttta tcactgataa gttggtggac atattatgtt
     1381 tatcagtgat aaagtgtcaa gcatgacaaa gttgcagccg aatacagtga tccgtgccgc
     1441 cctggacctg ttgaacgagg tcggcgtaga cggtctgacg acacgcaaac tggcggaacg
     1501 gttgggggtt cagcagccgg cgctttactg gcacttcagg aacaagcggg cgctgctcga
     1561 cgcactggcc gaagccatgc tggcggagaa tcatacgcat tcggtgccga gagccgacga
     1621 cgactggcgc tcatttctga tcgggaatgc ccgcagcttc aggcaggcgc tgctcgccta
     1681 ccgcgatggc gcgcgcatcc atgccggcac gcgaccgggc gcaccgcaga tggaaacggc
     1741 cgacgcgcag cttcgcttcc tctgcgaggc gggtttttcg gccggggacg ccgtcaatgc
     1801 gctgatgaca atcagctact tcactgttgg ggccgtgctt gaggagcagg ccggcgacag
     1861 cgatgccggc gagcgcggcg gcaccgttga acaggctccg ctctcgccgc tgttgcgggc
     1921 cgcgatagac gccttcgacg aagccggtcc ggacgcagcg ttcgagcagg gactcgcggt
     1981 gattgtcgat ggattggcga aaaggaggct cgttgtcagg aacgttgaag gaccgagaaa
     2041 gggtgacgat tgatcaggac cgctgccgga gcgcaaccca ctcactacag cagagccatg
     2101 tagacaacat cccctccccc tttccaccgc gtcagacgcc cgtagcagcc cgctacgggc
     2161 tttttcatgc cctgccctag cgtccaagcc tcacggccgc gctcggcctc tctggcggcc
     2221 ttctggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2281 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2341 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2401 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2461 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2521 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2581 ccttcgggaa gcgtggcgct tttccgctgc ataaccctgc ttcggggtca ttatagcgat
     2641 tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt
     2701 agactttcct tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc
     2761 cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa
     2821 tcctgctctg cgaggctggc cggctaccgc cggcgtaaca gatgagggca agcggatggc
     2881 tgatgaaacc aagccaacca ggaagggcag cccacctatc aaggtgtact gccttccaga
     2941 cgaacgaaga gcgattgagg aaaaggcggc ggcggccggc atgagcctgt cggcctacct
     3001 gctggccgtc ggccagggct acaaaatcac gggcgtcgtg gactatgagc acgtccgcga
     3061 gctggcccgc atcaatggcg acctgggccg cctgggcggc ctgctgaaac tctggctcac
     3121 cgacgacccg cgcacggcgc ggttcggtga tgccacgatc ctcgccctgc tggcgaagat
     3181 cgaagagaag caggacgagc ttggcaaggt catgatgggc gtggtccgcc cgagggcaga
     3241 gccatgactt ttttagccgc taaaacggcc ggggggtgcg cgtgattgcc aagcacgtcc
     3301 ccatgcgctc catcaagaag agcgacttcg cggagctggt gaagtacatc accgacgagc
     3361 aaggcaagac cgagcgcctt tccgacgctc accgggctgg ttgccctcgc cgctgggctg
     3421 gcggccgtct atggccctgc aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac
     3481 cgcggccgcc ggcgttgtgg atacctcgcg gaaaacttgg ccctcactga cagatgaggg
     3541 gcggacgttg acacttgagg ggccgactca cccggcgcgg cgttgacaga tgaggggcag
     3601 gctcgatttc ggccggcgac gtggagctgg ccagcctcgc aaatcggcga aaacgcctga
     3661 ttttacgcga gtttcccaca gatgatgtgg acaagcctgg ggataagtgc cctgcggtat
     3721 tgacacttga ggggcgcgac tactgacaga tgaggggcgc gatccttgac acttgagggg
     3781 cagagtgctg acagatgagg ggcgcaccta ttgacatttg aggggctgtc cacaggcaga
     3841 aaatccagca tttgcaaggg tttccgcccg tttttcggcc accgctaacc tgtcttttaa
     3901 cctgctttta aaccaatatt tataaacctt gtttttaacc agggctgcgc cctgtgcgcg
     3961 tgaccgcgca cgccgaaggg gggtgccccc ccttctcgaa ccctcccggc ccgctaacgc
     4021 gggcctccca tccccccagg ggctgcgccc ctcggccgcg aacggcctca ccccaaaaat
     4081 ggcagcgctg gcagtccttg ccattgccgg gatcggggca gtaacgggat gggcgatcag
     4141 cccgagcgcg acgcccggaa gcattgacgt gccgcaggtg ctggcatcga cattcagcga
     4201 ccaggtgccg ggcagtgagg gcggcggcct gggtggcggc ctgcccttca cttcggccgt
     4261 cggggcattc acggacttca tggcggggcc ggcaattttt accttgggca ttcttggcat
     4321 agtggtcgcg ggtgccgtgc tcgtgttcgg gggtgcgata aacccagcga accatttgag
     4381 gtgataggta agattatacc gaggtatgaa aacgagaatt ggacctttac agaattactc
     4441 tatgaagcgc catatttaaa aagctaccaa gacgaagagg atgaagagga tgaggaggca
     4501 gattgccttg aatatattga caatactgat aagataatat atcttttata tagaagatat
     4561 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
     4621 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaaccagga caataacctt
     4681 atagcttgta aattctatca taattgggta atgactccaa cttattgata gtgttttatg
     4741 ttcagataat gcccgatgac tttgtcatgc agctccaccg attttgagaa cgacagcgac
     4801 ttccgtccca gccgtgccag gtgctgcctc agattcaggt tatgccgctc aattcgctgc
     4861 gtatatcgct tgctgattac gtgcagcttt cccttcaggc gggattcata cagcggccag
     4921 ccatccgtca tccatatcac cacgtcaaag ggtgacagca ggctcataag acgccccagc
     4981 gtcgccatag tgcgttcacc gaatacgtgc gcaacaaccg tcttccggag actgtcatac
     5041 gcgtaaaaca gccagcgctg gcgcgattta gccccgacat agccccactg ttcgtccatt
     5101 tccgcgcaga cgatgacgtc actgcccggc tgtatgcgcg aggttaccga ctgcggcctg
     5161 agttttttaa gtgacgtaaa atcgtgttga ggccaacgcc cataatgcgg gctgttgccc
     5221 ggcatccaac gccattcatg gccatatcaa tgattttctg gtgcgtaccg ggttgagaag
     5281 cggtgtaagt gaactgcagt tgccatgttt tacggcagtg agagcagaga tagcgctgat
     5341 gtccggcggt gcttttgccg ttacgcacca ccccgtcagt agctgaacag gagggacagc
     5401 tgatagaaac agaagccact ggagcacctc aaaaacacca tcatacacta aatcagtaag
     5461 ttggcagcat cacccataat tgtggtttca aaatcggctc cgtcgatact atgttatacg
     5521 ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt tagaatgcaa
     5581 ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc tttaaatact
     5641 gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg aattgaaaaa
     5701 actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg ctaaggtata
     5761 taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc ggtataaagg
     5821 gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag gaaagctgcc
     5881 tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc tcatgagtga
     5941 ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg aaaagattat
     6001 cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg attgtcccta
     6061 tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata acgatctggc
     6121 cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc gcgagctgta
     6181 tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg gcgacctggg
     6241 agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc ttgggagaag
     6301 cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca gggaggatat
     6361 cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga
     6421 gaaaataaaa tattatattt tactggatga attgttttag tacctagatg tggcgcaacg
     6481 atgccggcga caagcaggag cgcaccgact tcttccgcat caagtgtttt ggctctcagg
     6541 ccgaggccca cggcaagtat ttgggcaagg ggtcgctggt attcgtgcag ggcaagattc
     6601 ggaataccaa gtacgagaag gacggccaga cggtctacgg gaccgacttc attgccgata
     6661 aggtggatta tctggacacc aaggcaccag gcgggtcaaa tcaggaataa gggcacattg
     6721 ccccggcgtg agtcggggca atcccgcaag gagggtgaat gaatcggacg tttgaccgga
     6781 aggcatacag gcaagaactg atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg
     6841 caagccgcac cgtcatgcgt gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc
     6901 agcaagctac ggccaagatc gagcgcgaca gcgtgcaact ggctccccct gccctgcccg
     6961 cgccatcggc cgccgtggag cgttcgcgtc gtctcgaaca ggaggcggca ggtttggcga
     7021 agtcgatgac catcgacacg cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg
     7081 aggacctggc aaaacaggtc agcgaggcca agcaggccgc gttgctgaaa cacacgaagc
     7141 agcagatcaa ggaaatgcag ctttccttgt tcgatattgc gccgtggccg gacacgatgc
     7201 gagcgatgcc aaacgacacg gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc
     7261 cgcgcgaggc gctgcaaaac aaggtcattt tccacgtcaa caaggacgtg aagatcacct
     7321 acaccggcgt cgagctgcgg gccgacgatg acgaactggt gtggcagcag gtgttggagt
     7381 acgcgaagcg cacccctatc ggcgagccga tcaccttcac gttctacgag ctttgccagg
     7441 acctgggctg gtcgatcaat ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc
     7501 tacaggcgac ggcgatgggc ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc
     7561 tgctgcaccg cttccgcgtc ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga
     7621 tcgacgagga aatcgtcgtg ctgtttgctg gcgaccacta cacgaaattc atatgggaga
     7681 agtaccgcaa gctgtcgccg acggcccgac ggatgttcga ctatttcagc tcgcaccggg
     7741 agccgtaccc gctcaagctg gaaaccttcc gcctcatgtg cggatcggat tccacccgcg
     7801 tgaagaagtg gcgcgagcag gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg
     7861 tggaacacgc ctgggtcaat gatgacctgg tgcattgcaa acgctagggc cttgtggggt
     7921 cagttccggc tgggggttca gcagccagcg ctttactggc atttcaggaa caagcgggca
     7981 ctgctcgacg cacttgcttc gctcagtatc gctcgggacg cacggcgcgc tctacgaact
     8041 gccgataaac agaggattaa aattgacaat tgtgattaag gctcagattc gacggcttgg
     8101 agcggccgac gtgcaggatt tccgcgagat ccgattgtcg gccctgaaga aagctccaga
     8161 gatgttcggg tccgtttacg agcacgagga gaaaaagccc atggaggcgt tcgctgaacg
     8221 gttgcgagat gccgtggcat tcggcgccta catcgacggc gagatcattg ggctgtcggt
     8281 cttcaaacag gaggacggcc ccaaggacgc tcacaaggcg catctgtccg gcgttttcgt
     8341 ggagcccgaa cagcgaggcc gaggggtcgc cggtatgctg ctgcgggcgt tgccggcggg
     8401 tttattgctc gtgatgatcg tccgacagat tccaacggga atctggtgga tgcgcatctt
     8461 catcctcggc gcacttaata tttcgctatt ctggagcttg ttgtttattt cggtctaccg
     8521 cctgccgggc ggggtcgcgg cgacggtagg cgctgtgcag ccgctgatgg tcgtgttcat
     8581 ctctgccgct ctgctaggta gcccgatacg attgatggcg gtcctggggg ctatttgcgg
     8641 aactgcgggc gtggcgctgt tggtgttgac accaaacgca gcgctagatc ctgtcggcgt
     8701 cgcagcgggc ctggcggggg cggtttccat ggcgttcgga accgtgctga cccgcaagtg
     8761 gcaacctccc gtgcctctgc tcacctttac cgcctggcaa ctggcggccg gaggacttct
     8821 gctcgttcca gtagctttag tgtttgatcc gccaatcccg atgcctacag gaaccaatgt
     8881 tctcggcctg gcgtggctcg gcctgatcgg agcgggttta acctacttcc tttggttccg
     8941 ggggatctcg cgactcgaac ctacagttgt ttccttactg ggctttctca gccccagatc
     9001 tggggtcgat cagccgggga tgcatcaggc cgacagtcgg aacttcgggt ccccgacctg
     9061 taccattcgg tgagcaatgg ataggggagt tgatatcgtc aacgttcact tctaaagaaa
     9121 tagcgccact cagcttcctc agcggcttta tccagcgatt tcctattatg tcggcatagt
     9181 tctcaagatc gacagcctgt cacggttaag cgagaaatga ataagaaggc tgataattcg
     9241 gatctctgcg agggagatga tatttgatca caggcagcaa cgctctgtca tcgttacaat
     9301 caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc
     9361 ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga
     9421 cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
     9481 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta
     9541 gacaacttaa taacacattg cggacgtttt taatgtactg gggtggtttt tcttttcacc
     9601 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag
     9661 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt tccgaaatcg
     9721 gcaaaatccc ttataaatca aaagaatagc ccgagatagg gttgagtgtt gttccagttt
     9781 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     9841 atcagggcga tggcccacta cgtgaaccat cacccaaatc aagttttttg gggtcgaggt
     9901 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     9961 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gccattcagg
    10021 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
    10081 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
    10141 cgttgtaaaa cgacggccag tgaattaatt cccatcttga aagaaatata gtttaaatat
    10201 ttattgataa aataacaagt caggtattat agtccaagca aaaacataaa tttattgatg
    10261 caagtttaaa ttcagaaata tttcaataac tgattatatc agctggtaca ttgccgtaga
    10321 tgaaagactg agtgcgatat tatgtgtaat acataaattg atgatatagc tagcttagct
    10381 catcggggga tcgatccgtc gaagctagct tgggtcccgc tcagaagaac tcgtcaagaa
    10441 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
    10501 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
    10561 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
    10621 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
    10681 gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
    10741 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
    10801 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
    10861 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
    10921 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
    10981 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt
    11041 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
    11101 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
    11161 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atccaagctc
    11221 ccatgggccc tcgactagag tcgagatctg gattgagagt gaatatgaga ctctaattgg
    11281 ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc
    11341 tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag
    11401 ctcattaaac tccagaaacc cgcggctgag tggctccttc aacgttgcgg ttctgtcagt
    11461 tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa
    11521 ttctccgctc atgatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc
    11581 cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt
    11641 tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct
    11701 acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc
    11761 atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt cctttagcag
    11821 cccttgcgcc ctgagtgctt gcggcagcgt gaagcttcga cgagtcagta ataaacggcg
    11881 tcaaagtggt tgcagccggc acacacgagt cgtgtttatc aactcaaagc acaaatactt
    11941 ttcctcaacc taaaaataag gcaattagcc aaaaacaact ttgcgtgtaa acaacgctca
    12001 atacacgtgt cattttatta ttagctattg cttcaccgcc ttagctttct cgtgacctag
    12061 tcgtcctcgt cttttcttct tcttcttcta taaaacaata cccaaagagc tcttcttctt
    12121 cacaattcag atttcaattt ctcaaaatct taaaaacttt ctctcaattc tctctaccgt
    12181 gatcaaggta aatttctgtg ttccttattc tctcaaaatc ttcgattttg ttttcgttcg
    12241 atcccaattt cgtatatgtt ctttggttta gattctgtta atcttagatc gaagacgatt
    12301 ttctgggttt gatcgttaga tatcatctta attctcgatt agggtttcat agatatcatc
    12361 cgatttgttc aaataatttg agttttgtcg aataattact cttcgatttg tgatttctat
    12421 ctagttctgg tgttagtttc tagtttgtgc gatcgaattt gtcgattaat ctgagttttt
    12481 ctgattaaca gactagcccg cggaaaatga aggctcttac tgctagacaa caagaagttt
    12541 ttgatcttat tagagatcat atttctcaaa ctggaatgcc tcctactaga gctgaaattg
    12601 ctcaaagact tggatttagg tctcctaatg ctgctgaaga acatcttaag gctcttgcta
    12661 gaaagggagt tattgaaatt gtttctggag cttcaagagg aattagactt cttcaagaag
    12721 aagaagaagg acttcctctt gttggaagag ttgctgctgg agaaccttct tctgctcctc
    12781 ctactgatgt ttctcttgga gatgaacttc atcttgatgg agaagatgtt gctatggctc
    12841 atgctgatgc tcttgatgat tttgatcttg atatgcttgg agatggagat tctcctggac
    12901 ctggatttac tcctcatgat tctgctcctt atggagcact tgatatggct gattttgaat
    12961 ttgaacaaat gtttactgat gctcttggaa ttgatgaata tggaggagat ccttctgctg
    13021 gagatatgag agctgctaat ctttggcctt ctcctcttat gattaagagg tctaagaaga
    13081 actctcttgc tctttctctt actgctgacc aaatggtttc tgctcttctt gatgctgaac
    13141 ctcctattct ttattctgaa tatgatccta ctagaccttt ttctgaggct tctatgatgg
    13201 gacttcttac taatcttgct gatagagaac ttgttcatat gattaattgg gctaagagag
    13261 ttcctggatt tgttgatctt actcttcatg accaagttca tcttcttgaa tgtgcttggc
    13321 ttgaaattct tatgattgga cttgtttgga ggtctatgga acatcctgtt aaacttcttt
    13381 ttgctcctaa tcttcttctt gatagaaatc aaggaaagtg tgttgaagga atggttgaaa
    13441 tttttgatat gcttcttgct acttcttcaa gatttagaat gatgaatctt caaggagaag
    13501 aatttgtttg tcttaagtct attattcttc ttaattctgg agtttatact tttctttctt
    13561 ctactcttaa gtctcttgaa gaaaaggatc atattcatag agttcttgat aagattactg
    13621 atactcttat tcatcttatg gctaaggctg gacttactct tcaacaacaa catcaaagac
    13681 ttgctcaact tcttcttatt ctttctcata ttagacatat gtctaataag ggaatggaac
    13741 atctttattc tatgaagtgt aagaatgttg ttcctcttta tgatcttctt cttgaaatgc
    13801 ttgatgctca tagacttcat gctcctactt caagaggagg agcttctgtt gaagaaactg
    13861 accaatctca tcttgctact gctggatcta cttcttctca ttctcttcaa aagtattata
    13921 ttactggaga agctgaagga tttcctgcta ctgtttgaag atcttgatca agctccctgg
    13981 ccaattccca gagatgttag ctgaaatcat cactaatcag ataccaaaat attcaaatgg
    14041 aaatatcaaa tagcttctgt ttcatcaaaa atgactcgac ctaactgagt aagcatgctt
    14101 gttcgagtat tatggcattg ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat
    14161 ttactgtgtt ttttattcgg ttttcgctat cgaactgtga aatggaaatg gatggagaag
    14221 agttaatgaa tgatatggtc cttttgttca ttctcaaatt aatattattt gttttttctc
    14281 ttatttgttg tgtgttgaat ttgaaattat aagagatatg caaacatttt gttttgagta
    14341 aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat atgaggagta aaacatccca
    14401 aacgcggccg caaccggtct tgcatccagc tgggctgcag gtcgaggcta aaaaactaat
    14461 cgcattatca tcccctcgac gtactgtaca tataaccact ggttttatat acagcagtac
    14521 tgtacatata accactggtt ttatatacag cagtcgacgt a
//
