LOCUS       sequence-208480-        9853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the C. elegans acy-1 cDNA in body wall muscle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Reynolds NK, Schade MA, Miller KG
  TITLE     Convergent, RIC-8-dependent Galpha signaling pathways in the 
            Caenorhabditis elegans synaptic signaling network.
  JOURNAL   Genetics. 2005 Feb;169(2):651-70. doi: 10.1534/genetics.104.031286. 
            Epub 2004 Oct 16.
  PUBMED    15489511
REFERENCE   2  (bases 1 to 9853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.104.031286"; journalName: "Genetics"; date: 
            "2005-02"; volume: "169"; issue: "2"; pages: "651-70"
COMMENT     Sequence Label: KG#80
FEATURES             Location/Qualifiers
     source          1..9853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(7293..7315)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7415..7434
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7512..7530)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7598..7702
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7703..7771,7772..8563)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7921..7940)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8734..9322
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9223..9242
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9476..9493
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9610..9631
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9646..9651,9652..9669,9670..9676)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9684..9700
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9689..9711
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9708..9724
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9708..9724
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcaggtcgg ctataataag ttcttgaata aaataatttt cccgacaaaa
       61 catgagtatt tctttcgaaa ataaaagtgc aggctaatta gagattattc tgtaattaac
      121 tgcataattt gtcacgtgcc atagttttac attccactac gtcatagttc ttaaaatact
      181 aatctcctga aaatagaagt aggtgaagaa agtttaatta tcagttctaa aatgacaatt
      241 gatctttgga atatgttctg aaactaccga tcattgaaca gatgctattt gaatgatata
      301 gaattgtata tttgcaattt ctgaaacgcg ttcttaaagg cacacagatt aattcaaaag
      361 ggtctggccg caaaaaggtt tatggtggcc gattttgagt tttgtgtgtg attgcttttt
      421 cacaatcagt gttttcagga ttatgtgatg aactagatct tcaagtttcg ttacatttca
      481 tatgttttcg gaactcacga agtacatatt gggtattgtg ctcaaaaaat tcagcaatca
      541 gcttcgctcc gctgacttta gaacccaaaa aaatagtatg gccaaactga ctgtgttacg
      601 atcatttcaa tttttcaata catatttaag atttctaaga gtaagaaggt caaaaactgt
      661 tctggaatac atatatattt ttcaggttac aattagtcaa aaagtgcact gaaatatacg
      721 ttttaatttc acgaataacc caattagttc aatgtatttt tggtcaacca acgttaaagt
      781 ttggcttcca accaattatc atttctgatc aaccacaatg ttttttcttt atctgcaagt
      841 taatttttta tttttatcca gatgtttggc atatttttca attcttcact agcgcccact
      901 tcttgcactt ccggcgccct gaatctaatg catctgttgc aagaattgaa agaccaatca
      961 acacattgtt ttcttcacga gatactgaag aaaatgaata aaaacagaga aaaagagcca
     1021 tgtgattagt gacaactgtt gctaacagat accatagctt ggacttggta cgtgatggca
     1081 acgtatgggt caacaaaaat gattgcagag ggggtgcaaa acagtcaagt cgagaaaata
     1141 tgaaaaacag aaaacaaaga acagaaaaat gggtttgaga gtcagtataa tttataaaag
     1201 aaaaattgta catagaaatt aaccattttt gtagaagaag ttatttttca agcatcgtta
     1261 aaaattattc aaagcacctt atttcatatt taattttaaa catggttaaa tgaacaacac
     1321 ggtgcgcaat caggaaaact tgaaatctga aactgttgtt gtgatcttct tcgcaactgt
     1381 tcagatagca ctagtgtaat gttaagagtg cgcgaatata atggaatata atggatcaca
     1441 cctcctgcca tcaggtaaac gtctctgtta tcacatattt ccaactatta aatttttacc
     1501 ttttacagtt ttacattttt ttgaaaaaag taactttttg tcttcaaaat ccctgacgaa
     1561 aatatcaaat attttaatcg agactgcaga ggaaccgatt gatgatttgg aaaatccagc
     1621 tttacctgtg taagaactga aaagtttcat aaccctaggg tattcccagt tacattcccc
     1681 actggctaac aatagcaccc agtttttcat cacctttctt caaatttctc ggcgatttgt
     1741 taaaaacaaa atttgtgtcc cttctctgat atctctatgt ctctaaacac aagttcatcg
     1801 gaaaacgaag gagggtaggt gttggttggg ctcccgaagt gaaaatagaa gagcaagaat
     1861 agaatattag agagagagtg cagagagggc gggatagctc ccgggattcc gttttcttct
     1921 tctttatctt caacgatgat gtgtgtgcgt gttgtataga ttctgttgct cccccacaac
     1981 tcgctccgaa ggctcaatac aattcaattg atattggagg agagcctacc ggagtgggag
     2041 gataagaaga aacataagaa gaagaagaag aagaagcatg cttctggttt ttgatgctat
     2101 gaaaacggca caaaaagatg attgaggtcc cttttcaata ccttctctca tctttcaaat
     2161 cccattgaaa cctaaaactt ctcaccacgc tttaccattg ttctccaaaa acttatagca
     2221 atgtctataa cttttttatc tctgaaaagc agtgttccat ttttcttttt cctattttat
     2281 ttcaattgtt tctcacattt cgtttggatt ctttgcttgt caaccagctt cttcttccac
     2341 ttttaccgtc taattttcag ggcagggagc catcaaaccc acgaccacta gatccatcta
     2401 gaggatcccc gggattggcc aaaggaccca aaggtatgtt tcgaatgata ctaacataac
     2461 atagaacatt ttcaggagga cccttggcta gcgtcgacgg taccggtaaa aatggacgac
     2521 gatgtcggtg aacgaacacc ggctctcggt ggttcatgtg gaccatcagt tcgtgcacac
     2581 agtagttccc caagacgtgt tccattattt gagagagcat ctgctagatg gtggaatcct
     2641 caatttcgat ctgcaacact agaagctcaa tattggaagt gtagtttcag tcagttaaga
     2701 gatcgatttc gttctggatt aatatatatt gccgttgtaa ttgccgcttg gacactttat
     2761 ttagctttat tcgatagaac ttttattcag cattggatcg tttcactatg cctctgtgcc
     2821 attattttcg cgatgtttgc cttcacggcg tgcgccgctc aataccaaag gttctacatg
     2881 ccaacctcat ttctatgcac ttttttgata tgccttgtca cgttgctcat cttctccgca
     2941 gagaatcaag ctgcgtttat gactccagtt gcaagtcttg ccacaagttt tcaggtcgtt
     3001 ctcctgatct acacagtcat tccactgcca ctctatttat gtatactcat cggaataatc
     3061 tactcaatcc tatttgagat tctcaacaag aacaaaattg gtcttgaaga ggccggatac
     3121 attaagcttg tccttcacgc tggtgtccat ttactcggtg tccatttgtt cattttaact
     3181 caagtgcgac agaggaaaac gtttctgaaa gtgggacaat cgatgttggc aagaaaggat
     3241 ctagagttgg agacacagtt taaagatcat atgattcagt ctgtgatgcc taaaaaggta
     3301 gctgatgaat tactgaaaga tgcatcagaa cttcgccgac cttctgcttc caacgactca
     3361 aactgccgaa cttcaaatgc tacacaagtt gaccagcccc ttgccaagat ggtacctgaa
     3421 taccgaaagt tccgtccatt tacgatgaat ctcatgacaa atgtgtcgat tctgtttgcg
     3481 gatattgctg ggtttactaa aatgtcatca aataaatctg ccgacgaact tgtcaatctg
     3541 ctcaatgatc tttttggaag attcgatact ttgtgtcgat tgagaggatt agagaagatt
     3601 tcaactttgg gagattgtta ctattgtgtt gctggatgtc ctgaaccatg tgatgatcat
     3661 gcatgcagaa cagtcgaaat gggtctcgac atgattgttg caatccgcca atttgatatt
     3721 gatcgtggac aagaagtgaa tatgcgtgtg ggaattcata caggaaaagt aatgtgcgga
     3781 atggttggaa caaaaagatt caaattcgat gtgttctcaa acgatgtcac actggcaaat
     3841 gagatggaat cttctggtgt tgctggaaga gttcacgttt ccgaagcaac tgcaaaactt
     3901 ttgaaagggc tttatgaaat tgaagaggga ccggattatg atggtccatt gaggatgcaa
     3961 gttcagggaa ctgaacgtcg tgttaaacca gaaagcatga agactttctt tatcaaagga
     4021 cgtattaatg atggtgtaga agaagaagtg atgcaagttc aggaagttga gagccttcac
     4081 agtcagaaaa gcagtaaaaa gtcgactcta aaacagaaat gggcggaaaa gttgaaaatg
     4141 aaccacacaa attcttatcc aatgcgagcc gctgcacgtg aaggaggtgg aagtttgaga
     4201 ataaaattgg cagagagaaa tagaagtaca cagttactgc caaaagaatc aaattcaatt
     4261 tgtataatgg aagataaccg aaagtcagca tcacttcaag cattggcaac gaataatttt
     4321 aatggaagta atacagatac aaataacact tatagtgaac gtggagttgc tggaagtgtt
     4381 agtaagaagt ctgtagctgg aagtgaaagt aatagtataa aaggaagtag aagcagtgga
     4441 ctacaattat ctctacaaga tggaaattct gatttaaatt cagttggagg tttggatact
     4501 gcaatcagtc accatcacaa tgcagcatct ctcactcgat ttgatactga taacaatttt
     4561 gatcagcgtt tggcaatggt aattggacaa ggagaaggag gatttgataa aggattttgg
     4621 aatcatcatg atagtttgaa taagtggact cttcgattta atgaaaagga cgttgaagag
     4681 gaatatcgag ctcattttgt ggattcatcg gaaagatata cggcatcaaa gaaaggtcat
     4741 gttgaaagac ataaagattt gatggaacaa ggtggagaaa aagatggaat tactggatca
     4801 acggtcaaca agtatagata ttcaggagtc ttcatcgata taattgttgc cacgttgata
     4861 tttgtaatat cgggtgcagt tgcaattatg tctgtccgtc cattcccatt atcactcatt
     4921 gcatacttcc catttgctgc tgcaattctc attcttacaa ttgtccttat tggtcttcca
     4981 cttctcgccc gtaaaaaatc attccaatgt gcaaatcaat ggatgccacg tcatctcatc
     5041 ggtcttcttc tgatctttct tccaatcggt gtcgcaatct gtatcatgcc attgtgccaa
     5101 agtggagact gtgcaaatgt aattctgaac taccgtcttg cattcagtta tgtcaccatt
     5161 cttgccatat tcgctcattg caacttctcc caattagctg cctggccaaa aactactgct
     5221 gcagtattta tcggacttct ccacattgct ggagttttct actgcgaatt caatttgaaa
     5281 cacttagttg aagagcaaga cacctgcaac gtgacagcaa tcatgattcc accaattcgc
     5341 aaaggtctga actacacaat tgcattgaac tcgacatctg caagaacatt atctcaagat
     5401 tttggatctc cattattcat ttgggagctg cttctggatg tgatcctgtc gattgttcta
     5461 gttgcattcc ttaattatca gttcgaaacc gcattccgta tgtcattctt tggagatgtt
     5521 caagctcgtc gggacactga aagaatgcaa atcgtgcgtg atcaagccga ttggctcctg
     5581 aataacgtga tcccagccca cgcagtagaa agtcttaaaa cggataccaa atacagtgaa
     5641 aaccatgaaa cagttggtgt actgttcgct tcaatcacta attggaatga tatgtatgaa
     5701 gagaatttcg aagggggcag agaattcttg agagttctca atgaagttat tggtgacttt
     5761 gatgagcttc tcgatcggcc ggacttcacg catattgaaa aaatcaaaac aattgggcct
     5821 gcgtatatgg cagcaagtgg attgaatcca gaacgaaaaa agaatatgtt gcatccaaaa
     5881 gagcatctat atcagatggt tgattttgca ttggcagttc aacatgtact cagtgttttc
     5941 aacgaggatt tgctcaattt tgattttgtt tgcaagttag gacttaatat tggacccgtc
     6001 actgctggtg tcattggaac aacaaagttg tactacgata tctggggtga cactgtaaac
     6061 attgctagcc gaatgtacag caccggagtt ttaaatcgca ttcaagtatc acaacacaca
     6121 cgagaatatc tcctggatcg gtatgaattc gagttccgcg atcatattga agtcaaggga
     6181 attgatggag gaatggatac ttatcttttg gttggtcgaa aaggtgatgg aatacctcca
     6241 tcaatcaagg ataaccaaga agacgaattt taaatctcga ggagctcccg agatcttcag
     6301 cgctcagcat tcgtagaatt gtaagtttaa actttctcat actaactaac gcgctctatt
     6361 taaattttca gaattccaac tgagcgccgg tcgctaccat taccaacttg tctggtgtca
     6421 aaaataatag gggccgctgt catcagagta agtttaaact gagttctact aactaacgag
     6481 taatatttaa attttcagca tctcgcgccc gtgcctctga cttctaagtc caattactct
     6541 tcaacatccc tacatgctct ttctccctgt gctcccaccc cctatttttg ttattatcaa
     6601 aaaaacttct tcttaatttc tttgtttttt agcttctttt aagtcacctc taacaatgaa
     6661 attgtgtaga ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc
     6721 ctccccccat taataataat tctatcccaa aatctacaca atgttctgtg tacacttctt
     6781 atgttttttt tacttctgat aaattttttt tgaaacatca tagaaaaaac cgcacacaaa
     6841 ataccttatc atatgttacg tttcagttta tgaccgcaat ttttatttct tcgcacgtct
     6901 gggcctctca tgacgtcaaa tcatgctcat cgtgaaaaag ttttggagta tttttggaat
     6961 ttttcaatca agtgaaagtt tatgaaatta attttcctgc ttttgctttt tgggggtttc
     7021 ccctattgtt tgtcaagagt ttcgaggacg gcgtttttct tgctaaaatc acaagtattg
     7081 atgagcacga tgcaagaaag atcggaagaa ggtttgggtt tgaggctcag tggaaggtga
     7141 gtagaagttg ataatttgaa agtggagtag tgtctatggg gtttttgcct taaatgacag
     7201 aatacattcc caatatacca aacataactg tttcctacta gtcggccgta cgggcccttt
     7261 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     7321 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     7381 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     7441 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     7501 cggccttaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     7561 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     7621 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     7681 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     7741 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     7801 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     7861 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     7921 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     7981 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     8041 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     8101 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     8161 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     8221 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     8281 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     8341 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     8401 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     8461 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     8521 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     8581 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     8641 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     8701 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     8761 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     8821 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     8881 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     8941 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     9001 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     9061 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     9121 tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     9181 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     9241 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     9301 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     9361 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     9421 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     9481 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     9541 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     9601 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     9661 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     9721 tgaccatgat tacgccaagc tgtaagttta aacatgatct tactaactaa ctattctcat
     9781 ttaaattttc agagcttaaa aatggctgaa atcactcaca acgatggata cgctaacaac
     9841 ttggaaatga aat
//
