LOCUS       sequence-208484-        9402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from 
            the Ptac promoter for expression in P. putida and P. fluorescens.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Tan SZ, Reisch CR, Prather KLJ
  TITLE     A Robust CRISPR Interference Gene Repression System in Pseudomonas.
  JOURNAL   J Bacteriol. 2018 Mar 12;200(7). pii: JB.00575-17. doi: 
            10.1128/JB.00575-17. Print 2018 Apr 1.
  PUBMED    29311279
REFERENCE   2  (bases 1 to 9402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol."; date: "2018-03-12"; volume: "200(7). pii: JB.00575-17. 
            doi"; pages: " 10.1128/JB.00575-17. Print 2018 Apr 1"
COMMENT     Sequence Label: pUC18-mini-Tn7T-Lac-dCas9 (Ptac)
FEATURES             Location/Qualifiers
     source          1..9402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     100..119
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     533..549
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     534..550
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      578..672
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      775..861
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    907..954
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1084..1617)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1806..1811,1812..1828,1829..1834))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    1875..1922
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1965..3047)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3009..3028
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3048..3125)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        join(3355..3360,3361..3376,3377..3383)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    3391..3407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3396..3418
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             3459..3485
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     mobile_element  7097..7262
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      complement(7532..8120)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7612..7631)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8291..9082,9083..9151))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8914..8933
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9152..9256)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9324..9342
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9380..9402)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
       61 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
      121 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
      181 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
      241 cttcgctatt acgccagcta gaggaccagc cgcgtaacct ggcaaaatcg gttacggttg
      301 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
      361 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
      421 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
      481 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggg gttcgaggtc
      541 gacggtatcg ataagctagc ttaattagct gagcttggac tcctgttgat agatccagta
      601 atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt
      661 attggtgaga atccaagcta gactgcgatg agtggcaggg cggggcgtaa tttttttaag
      721 gcagttattg gtgcccttaa acgcctgggg taatgactct ctagcttgag gcatcaaata
      781 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
      841 gctctcctga gtaggacaaa tccgccgcta ggagcttgcg gcccggacga tcgaattggg
      901 gatcttgaag tacctattcc gaagttccta ttctctagaa agtataggaa cttcagagcg
      961 cttttgaagc tgatgtgctt aaaaacttac tcaatggaat tctagatcga attggccgcg
     1021 gcgttgtgac aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa
     1081 ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa
     1141 ctttgtatag agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata
     1201 agcaccaagc gcgttggcct catgcttgag gagattgatg agcgcggtgg caatgccctg
     1261 cctccggtgc tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc
     1321 aaacttgggc agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc
     1381 aagcgcgatg aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg
     1441 ttgggagtag gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat
     1501 ggatttgact tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc
     1561 taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt
     1621 tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc
     1681 gaggcataga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt
     1741 taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc
     1801 attacagttt acgaaccgaa caggcttatg tcaattcgat ccattgctgt tgacaaaggg
     1861 aatcagggga tcttgaagtt cctattccga agttcctatt ctctagaaag tataggaact
     1921 tcagagcgct tttgaagcta attcgatcat gcattgcgtt gcgctcactg cccgctttcc
     1981 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     2041 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga
     2101 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc
     2161 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg
     2221 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg
     2281 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt aggaacgatg
     2341 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc
     2401 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc
     2461 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat
     2521 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg
     2581 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc
     2641 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc
     2701 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac
     2761 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac
     2821 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc
     2881 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt
     2941 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa
     3001 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg
     3061 aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgca ccattcgatg
     3121 gtgtcaacgt aaatgccgct tcgccttcgc gcgcgaattg caagctgatc cgggcttatc
     3181 gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct
     3241 gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata
     3301 atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca
     3361 attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     3421 acagaattcg agctcctgac tcaggaggta tcgatatgta cccatacgat gttccagatt
     3481 acgctatgac caacggcaag atcctgggcc tggccatcgg catcgcctcg gtgggcgtgg
     3541 gcatcatcga ggccaagacc ggcaaggtgg tgcacgccaa ctcgcgcctg ttctcggccg
     3601 ccaacgccga gaacaacgcc gagcgccgcg gcttccgcgg ctcgcgccgc ctgaaccgcc
     3661 gcaagaagca ccgcgtgaag cgcgtgcgcg acctgttcga gaagtacggc atcgtgaccg
     3721 acttccgcaa cctgaacctg aacccgtacg agctgcgcgt gaagggcctg accgagcagc
     3781 tgaagaacga ggagctgttc gccgccctgc gcaccatctc gaagcgccgc ggcatctcgt
     3841 acctggacga cgccgaggac gactcgaccg gctcgaccga ctacgccaag tcgatcgacg
     3901 agaaccgccg cctgctgaag aacaagaccc cgggccagat ccagctggag cgcctggaga
     3961 agtacggcca gctgcgcggc aacttcaccg tgtacgacga gaacggcgag gcccaccgcc
     4021 tgatcaacgt gttctcgacc tcggactacg agaaggaggc ccgcaagatc ctggagaccc
     4081 aggccgacta caacaagaag atcaccgccg agttcatcga cgactacgtg gagatcctga
     4141 cccagaagcg caagtactac cacggcccgg gcaacgagaa gtcgcgcacc gactacggcc
     4201 gcttccgcac cgacggcacc accctggaga acatcttcgg catcctgatc ggcaagtgca
     4261 acttctaccc ggacgagtac cgcgcctcga aggcctcgta caccgcccag gagtacaact
     4321 tcctgaacga cctgaacaac ctgaaggtgt cgaccgagac cggcaagctg tcgaccgagc
     4381 agaaggagtc gctggtggag ttcgccaaga acaccgccac cctgggcccg gccaagctgc
     4441 tgaaggagat cgccaagatc ctggactgca aggtggacga gatcaagggc taccgcgagg
     4501 acgacaaggg caagccggac ctgcacacct tcgagccgta ccgcaagctg aagttcaacc
     4561 tggagtcgat caacatcgac gacctgtcgc gcgaggtgat cgacaagctg gccgacatcc
     4621 tgaccctgaa caccgagcgc gagggcatcg aggacgccat caagcgcaac ctgccgaacc
     4681 agttcaccga ggagcagatc tcggagatca tcaaggtgcg caagtcgcag tcgaccgcct
     4741 tcaacaaggg ctggcactcg ttctcggcca agctgatgaa cgagctgatc ccggagctgt
     4801 acgccacctc ggacgagcag atgaccatcc tgacccgcct ggagaagttc aaggtgaaca
     4861 agaagtcgtc gaagaacacc aagaccatcg acgagaagga ggtgaccgac gagatctaca
     4921 acccggtggt ggccaagtcg gtgcgccaga ccatcaagat catcaacgcc gccgtgaaga
     4981 agtacggcga cttcgacaag atcgtgatcg agatgccgcg cgacaagaac gccgacgacg
     5041 agaagaagtt catcgacaag cgcaacaagg agaacaagaa ggagaaggac gacgccctga
     5101 agcgcgccgc ctacctgtac aactcgtcgg acaagctgcc ggacgaggtg ttccacggca
     5161 acaagcagct ggagaccaag atccgcctgt ggtaccagca gggcgagcgc tgcctgtact
     5221 cgggcaagcc gatctcgatc caggagctgg tgcacaactc gaacaacttc gagatcgacg
     5281 ccatcctgcc gctgtcgctg tcgttcgacg actcgctggc caacaaggtg ctggtgtacg
     5341 cctggaccaa ccaggagaag ggccaaaaga ccccgtacca ggtgatcgac tcgatggacg
     5401 ccgcctggtc gttccgcgag atgaaggact acgtgctgaa gcagaagggc ctgggcaaga
     5461 agaagcgcga ctacctgctg accaccgaga acatcgacaa gatcgaggtg aagaagaagt
     5521 tcatcgagcg caacctggtg gacacccgct acgcctcgcg cgtggtgctg aactcgctgc
     5581 agtcggccct gcgcgagctg ggcaaggaca ccaaggtgtc ggtggtgcgc ggccagttca
     5641 cctcgcagct gcgccgcaag tggaagatcg acaagtcgcg cgagacctac caccaccacg
     5701 ccgtggacgc cctgatcatc gccgcctcgt cgcagctgaa gctgtgggag aagcaggaca
     5761 acccgatgtt cgtggactac ggcaagaacc aggtggtgga caagcagacc ggcgagatcc
     5821 tgtcggtgtc ggacgacgag tacaaggagc tggtgttcca gccgccgtac cagggcttcg
     5881 tgaacaccat ctcgtcgaag ggcttcgagg acgagatcct gttctcgtac caggtggact
     5941 cgaagtacaa ccgcaaggtg tcggacgcca ccatctactc gacccgcaag gccaagatcg
     6001 gcaaggacaa gaaggaggag acctacgtgc tgggcaagat caaggacatc tactcgcaga
     6061 acggcttcga caccttcatc aagaagtaca acaaggacaa gacccagttc ctgatgtacc
     6121 agaaggactc gctgacctgg gagaacgtga tcgaggtgat cctgcgcgac tacccgacca
     6181 ccaagaagtc ggaggacggc aagaacgacg tgaagtgcaa cccgttcgag gagtaccgcc
     6241 gcgagaacgg cctgatctgc aagtactcga agaagggcaa gggcaccccg atcaagtcgc
     6301 tgaagtacta cgacaagaag ctgggcaact gcatcgacat caccccggag gagtcgcgca
     6361 acaaggtgat cctgcagtcg atcaacccgt ggcgcgccga cgtgtacttc aacccggaga
     6421 ccctgaagta cgagctgatg ggcctgaagt actcggacct gtcgttcgag aagggcaccg
     6481 gcaactacca catctcgcag gagaagtacg acgccatcaa ggagaaggag ggcatcggca
     6541 agaagtcgga gttcaagttc accctgtacc gcaacgacct gatcctgatc aaggacatcg
     6601 cctcgggcga gcaggagatc taccgcttcc tgtcgcgcac catgccgaac gtgaaccact
     6661 acgtggagct gaagccgtac gacaaggaga agttcgacaa cgtgcaggag ctggtggagg
     6721 ccctgggcga ggccgacaag gtgggccgct gcatcaaggg cctgaacaag ccgaacatct
     6781 cgatctacaa ggtgcgcacc gacgtgctgg gcaacaagta cttcgtgaag aagaagggcg
     6841 acaagccgaa gctggacttc aagaacaaca agaagtaaat actctggcca tttttcttta
     6901 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc aacgcgtgcg
     6961 gccgctgatt agctaagcag aaggccatcc tgacggatgc ctttttcgct tgctagctgc
     7021 agcccggggg atcccccggg ctgcaggaat tcctcgagaa gcttgggccc ggtacctcgc
     7081 gaaggccttg caggccaacc agataagtga aatctagttc caaactattt tgtcattttt
     7141 aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct
     7201 aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca
     7261 cagcggggca tttttcttcc tgttatgttt gggcgctgca ttaatgaatc ggccaacgcg
     7321 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gacccgctgc
     7381 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7441 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7501 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7561 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7621 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7681 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7741 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7801 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7861 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7921 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7981 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8041 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8101 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8161 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8221 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8281 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8341 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8401 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8461 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8521 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8581 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8641 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8701 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8761 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8821 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8881 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8941 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9001 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9061 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9121 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9181 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9241 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     9301 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     9361 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc gg
//
