LOCUS       sequence-208486-        8673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the C. elegans acy--1 P260S gain-of-function cDNA 
            pan-neuronally.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Schade MA, Reynolds NK, Dollins CM, Miller KG
  TITLE     Mutations that rescue the paralysis of Caenorhabditis elegans ric-8 
            (synembryn) mutants activate the G alpha(s) pathway and define a 
            third major branch of the synaptic signaling network.
  JOURNAL   Genetics. 2005 Feb;169(2):631-49. doi: 10.1534/genetics.104.032334. 
            Epub 2004 Oct 16.
  PUBMED    15489510
REFERENCE   2  (bases 1 to 8673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.104.032334"; journalName: "Genetics"; date: 
            "2005-02"; volume: "169"; issue: "2"; pages: "631-49"
COMMENT     Sequence Label: KG#83
FEATURES             Location/Qualifiers
     source          1..8673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6113..6135)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6235..6254
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6332..6350)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6418..6522
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6523..6591,6592..7383)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6741..6760)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7554..8142
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8043..8062
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8296..8313
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8430..8451
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8466..8471,8472..8489,8490..8496)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8504..8520
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8509..8531
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8528..8544
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8528..8544
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcaggatct tcagatggga gcagtggact gtctatttga tattcttaaa
       61 atgtactcta taaaagatga aactgtaatg accaattccc tacataaata tttttaacta
      121 attggtcgga tatttgggga tcagagaagt acttgttttt gcgatattaa taatgatata
      181 tttttctagt aaaaaaatgt taagcgattt gtgaataatt aagttggctt gaaaaagatt
      241 ttttgatagc tatgtatttt gttaatttaa atcagatttt aaaactttat tattgactat
      301 ttatatatat tatatattat atattttcat ggtttaatca aaatctatta aattatttct
      361 aaaatctcta aatatcgaat tctctaaaaa gcttcatcaa caaaagcatt tcaattatct
      421 aacacaatga gaataattca agaactgaaa tagaaagaaa tctagaaaaa aaagagagtg
      481 aggtgcccta ttaaatcggt gctctcctct ctaacagaag cgcctacacg cacccaagag
      541 agctcttttt ctttcttttt tcggagcaca catacacgca tacactcctc agaagtgtat
      601 agagaaaatg ggagaagaag cgaaagaaga cgaagaagcg gttgtgatgt atgcatcggg
      661 cgtttctatc tctctccgtg agcaacgagc tagtcaaccc atcgatccat ttttgtgagc
      721 acacacagag agagactcaa acacactcac agcttcacac tgagctccaa gagctctgta
      781 gagagaagcc agagtatgct ttcctcgatt ttcctaacgt cttcttcttc tgcttttttc
      841 tttttcccaa cgagttgtag tccactaact tcaatttttc ctccagagaa agagttttag
      901 agagacagct gataaataca ctagagaaat aaaaagaatg ataaaaattt gctattttaa
      961 tcagttccct ctcgtttctc taagttttgt tgttgggttg ggaaattaaa attggaaaat
     1021 ggaaataacg tttttttctg tctaatcttg aattctccag acatttctta tttccttttt
     1081 tgttgcaagt ttttcttcct tctctaattt ttaatcaaag atatctcgct atgaactttg
     1141 aatttttgat attttgattc tataaataca taaagtaatc taaatgctct tttaaaataa
     1201 atctacagta gccctatttt caggatcccc gggattggcc aaaggaccca aaggtatgtt
     1261 tcgaatgata ctaacataac atagaacatt ttcaggagga cccttggcta gcgtcgacgg
     1321 taccggtaaa aatggacgac gatgtcggtg aacgaacacc ggctctcggt ggttcatgtg
     1381 gaccatcagt tcgtgcacac agtagttccc caagacgtgt tccattattt gagagagcat
     1441 ctgctagatg gtggaatcct caatttcgat ctgcaacact agaagctcaa tattggaagt
     1501 gtagtttcag tcagttaaga gatcgatttc gttctggatt aatatatatt gccgttgtaa
     1561 ttgccgcttg gacactttat ttagctttat tcgatagaac ttttattcag cattggatcg
     1621 tttcactatg cctctgtgcc attattttcg cgatgtttgc cttcacggcg tgcgccgctc
     1681 aataccaaag gttctacatg ccaacctcat ttctatgcac ttttttgata tgccttgtca
     1741 cgttgctcat cttctccgca gagaatcaag ctgcgtttat gactccagtt gcaagtcttg
     1801 ccacaagttt tcaggtcgtt ctcctgatct acacagtcat tccactgcca ctctatttat
     1861 gtatactcat cggaataatc tactcaatcc tatttgagat tctcaacaag aacaaaattg
     1921 gtcttgaaga ggccggatac attaagcttg tccttcacgc tggtgtccat ttactcggtg
     1981 tccatttgtt cattttaact caagtgcgac agaggaaaac gtttctgaaa gtgggacaat
     2041 cgatgttggc aagaaaggat ctagagttgg agacacagtt taaagatcat atgattcagt
     2101 ctgtgatgtc taaaaaggta gctgatgaat tactgaaaga tgcatcagaa cttcgccgac
     2161 cttctgcttc caacgactca aactgccgaa cttcaaatgc tacacaagtt gaccagcccc
     2221 ttgccaagat ggtacctgaa taccgaaagt tccgtccatt tacgatgaat ctcatgacaa
     2281 atgtgtcgat tctgtttgcg gatattgctg ggtttactaa aatgtcatca aataaatctg
     2341 ccgacgaact tgtcaatctg ctcaatgatc tttttggaag attcgatact ttgtgtcgat
     2401 tgagaggatt agagaagatt tcaactttgg gagattgtta ctattgtgtt gctggatgtc
     2461 ctgaaccatg tgatgatcat gcatgcagaa cagtcgaaat gggtctcgac atgattgttg
     2521 caatccgcca atttgatatt gatcgtggac aagaagtgaa tatgcgtgtg ggaattcata
     2581 caggaaaagt aatgtgcgga atggttggaa caaaaagatt caaattcgat gtgttctcaa
     2641 acgatgtcac actggcaaat gagatggaat cttctggtgt tgctggaaga gttcacgttt
     2701 ccgaagcaac tgcaaaactt ttgaaagggc tttatgaaat tgaagaggga ccggattatg
     2761 atggtccatt gaggatgcaa gttcagggaa ctgaacgtcg tgttaaacca gaaagcatga
     2821 agactttctt tatcaaagga cgtattaatg atggtgtaga agaagaagtg atgcaagttc
     2881 aggaagttga gagccttcac agtcagaaaa gcagtaaaaa gtcgactcta aaacagaaat
     2941 gggcggaaaa gttgaaaatg aaccacacaa attcttatcc aatgcgagcc gctgcacgtg
     3001 aaggaggtgg aagtttgaga ataaaattgg cagagagaaa tagaagtaca cagttactgc
     3061 caaaagaatc aaattcaatt tgtataatgg aagataaccg aaagtcagca tcacttcaag
     3121 cattggcaac gaataatttt aatggaagta atacagatac aaataacact tatagtgaac
     3181 gtggagttgc tggaagtgtt agtaagaagt ctgtagctgg aagtgaaagt aatagtataa
     3241 aaggaagtag aagcagtgga ctacaattat ctctacaaga tggaaattct gatttaaatt
     3301 cagttggagg tttggatact gcaatcagtc accatcacaa tgcagcatct ctcactcgat
     3361 ttgatactga taacaatttt gatcagcgtt tggcaatggt aattggacaa ggagaaggag
     3421 gatttgataa aggattttgg aatcatcatg atagtttgaa taagtggact cttcgattta
     3481 atgaaaagga cgttgaagag gaatatcgag ctcattttgt ggattcatcg gaaagatata
     3541 cggcatcaaa gaaaggtcat gttgaaagac ataaagattt gatggaacaa ggtggagaaa
     3601 aagatggaat tactggatca acggtcaaca agtatagata ttcaggagtc ttcatcgata
     3661 taattgttgc cacgttgata tttgtaatat cgggtgcagt tgcaattatg tctgtccgtc
     3721 cattcccatt atcactcatt gcatacttcc catttgctgc tgcaattctc attcttacaa
     3781 ttgtccttat tggtcttcca cttctcgccc gtaaaaaatc attccaatgt gcaaatcaat
     3841 ggatgccacg tcatctcatc ggtcttcttc tgatctttct tccaatcggt gtcgcaatct
     3901 gtatcatgcc attgtgccaa agtggagact gtgcaaatgt aattctgaac taccgtcttg
     3961 cattcagtta tgtcaccatt cttgccatat tcgctcattg caacttctcc caattagctg
     4021 cctggccaaa aactactgct gcagtattta tcggacttct ccacattgct ggagttttct
     4081 actgcgaatt caatttgaaa cacttagttg aagagcaaga cacctgcaac gtgacagcaa
     4141 tcatgattcc accaattcgc aaaggtctga actacacaat tgcattgaac tcgacatctg
     4201 caagaacatt atctcaagat tttggatctc cattattcat ttgggagctg cttctggatg
     4261 tgatcctgtc gattgttcta gttgcattcc ttaattatca gttcgaaacc gcattccgta
     4321 tgtcattctt tggagatgtt caagctcgtc gggacactga aagaatgcaa atcgtgcgtg
     4381 atcaagccga ttggctcctg aataacgtga tcccagccca cgcagtagaa agtcttaaaa
     4441 cggataccaa atacagtgaa aaccatgaaa cagttggtgt actgttcgct tcaatcacta
     4501 attggaatga tatgtatgaa gagaatttcg aagggggcag agaattcttg agagttctca
     4561 atgaagttat tggtgacttt gatgagcttc tcgatcggcc ggacttcacg catattgaaa
     4621 aaatcaaaac aattgggcct gcgtatatgg cagcaagtgg attgaatcca gaacgaaaaa
     4681 agaatatgtt gcatccaaaa gagcatctat atcagatggt tgattttgca ttggcagttc
     4741 aacatgtact cagtgttttc aacgaggatt tgctcaattt tgattttgtt tgcaagttag
     4801 gacttaatat tggacccgtc actgctggtg tcattggaac aacaaagttg tactacgata
     4861 tctggggtga cactgtaaac attgctagcc gaatgtacag caccggagtt ttaaatcgca
     4921 ttcaagtatc acaacacaca cgagaatatc tcctggatcg gtatgaattc gagttccgcg
     4981 atcatattga agtcaaggga attgatggag gaatggatac ttatcttttg gttggtcgaa
     5041 aaggtgatgg aatacctcca tcaatcaagg ataaccaaga agacgaattt taaatctcga
     5101 ggagctcccg agatcttcag cgctcagcat tcgtagaatt gtaagtttaa actttctcat
     5161 actaactaac gcgctctatt taaattttca gaattccaac tgagcgccgg tcgctaccat
     5221 taccaacttg tctggtgtca aaaataatag gggccgctgt catcagagta agtttaaact
     5281 gagttctact aactaacgag taatatttaa attttcagca tctcgcgccc gtgcctctga
     5341 cttctaagtc caattactct tcaacatccc tacatgctct ttctccctgt gctcccaccc
     5401 cctatttttg ttattatcaa aaaaacttct tcttaatttc tttgtttttt agcttctttt
     5461 aagtcacctc taacaatgaa attgtgtaga ttcaaaaata gaattaattc gtaataaaaa
     5521 gtcgaaaaaa attgtgctcc ctccccccat taataataat tctatcccaa aatctacaca
     5581 atgttctgtg tacacttctt atgttttttt tacttctgat aaattttttt tgaaacatca
     5641 tagaaaaaac cgcacacaaa ataccttatc atatgttacg tttcagttta tgaccgcaat
     5701 ttttatttct tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat cgtgaaaaag
     5761 ttttggagta tttttggaat ttttcaatca agtgaaagtt tatgaaatta attttcctgc
     5821 ttttgctttt tgggggtttc ccctattgtt tgtcaagagt ttcgaggacg gcgtttttct
     5881 tgctaaaatc acaagtattg atgagcacga tgcaagaaag atcggaagaa ggtttgggtt
     5941 tgaggctcag tggaaggtga gtagaagttg ataatttgaa agtggagtag tgtctatggg
     6001 gtttttgcct taaatgacag aatacattcc caatatacca aacataactg tttcctacta
     6061 gtcggccgta cgggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     6121 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     6181 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     6241 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     6301 ggagaaaata ccgcatcagg cggccttaag ggcctcgtga tacgcctatt tttataggtt
     6361 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     6421 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     6481 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     6541 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     6601 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     6661 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     6721 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     6781 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6841 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6901 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6961 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     7021 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     7081 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     7141 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     7201 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     7261 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     7321 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     7381 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     7441 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     7501 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7561 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7621 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7681 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     7741 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7801 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7861 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7921 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
     7981 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     8041 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     8101 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     8161 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     8221 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     8281 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     8341 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     8401 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     8461 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     8521 tttcacacag gaaacagcta tgaccatgat tacgccaagc tgtaagttta aacatgatct
     8581 tactaactaa ctattctcat ttaaattttc agagcttaaa aatggctgaa atcactcaca
     8641 acgatggata cgctaacaac ttggaaatga aat
//
