LOCUS       sequence-208487-        7957 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the C. elegans acy--1 P260S gain-of-function cDNA in 
            ventral cord cholinergic motor neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7957)
  AUTHORS   Schade MA, Reynolds NK, Dollins CM, Miller KG
  TITLE     Mutations that rescue the paralysis of Caenorhabditis elegans ric-8 
            (synembryn) mutants activate the G alpha(s) pathway and define a 
            third major branch of the synaptic signaling network.
  JOURNAL   Genetics. 2005 Feb;169(2):631-49. doi: 10.1534/genetics.104.032334. 
            Epub 2004 Oct 16.
  PUBMED    15489510
REFERENCE   2  (bases 1 to 7957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.104.032334"; journalName: "Genetics"; date: 
            "2005-02"; volume: "169"; issue: "2"; pages: "631-49"
COMMENT     Sequence Label: KG#84
FEATURES             Location/Qualifiers
     source          1..7957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(5397..5419)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5519..5538
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5616..5634)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5702..5806
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5807..5875,5876..6667)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6025..6044)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6838..7426
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7327..7346
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7580..7597
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7714..7735
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7750..7755,7756..7773,7774..7780)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7788..7804
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7793..7815
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7812..7828
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7812..7828
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagaagct tttggttttc acaattttct ggtttttggg atttttgcgg
       61 gaattttgag aggagaaagt tgggagagaa gatattggaa tggtccccgg ggagctgtac
      121 atttatacac caaggcaatc ttcgtaaaaa ataacaatag agtcatccct cagaacagaa
      181 aagaaacccg cccgggggct caaatacaca tgtcattttt cttttttcgc ctgattttta
      241 gcctaaatta gtgtttagca attttttaag gtaactcggt ggagtcattt gaacttttta
      301 aactaaaatt tcagaaaaaa ttcttttttt ttgtcaaaaa ataattttcc cgccggaatt
      361 tttcaaaaaa ataattttta ttttattttt caagttcctt gtttcctctt tattttttta
      421 ttttgccccc aaaagtttta cactaaaata tttaattttc aggtaattca tcgaaagtct
      481 ttctattttc cgcatctctt gttcaaggat ccccgggatt ggccaaagga cccaaaggta
      541 tgtttcgaat gatactaaca taacatagaa cattttcagg aggacccttg gctagcgtcg
      601 acggtaccgg taaaaatgga cgacgatgtc ggtgaacgaa caccggctct cggtggttca
      661 tgtggaccat cagttcgtgc acacagtagt tccccaagac gtgttccatt atttgagaga
      721 gcatctgcta gatggtggaa tcctcaattt cgatctgcaa cactagaagc tcaatattgg
      781 aagtgtagtt tcagtcagtt aagagatcga tttcgttctg gattaatata tattgccgtt
      841 gtaattgccg cttggacact ttatttagct ttattcgata gaacttttat tcagcattgg
      901 atcgtttcac tatgcctctg tgccattatt ttcgcgatgt ttgccttcac ggcgtgcgcc
      961 gctcaatacc aaaggttcta catgccaacc tcatttctat gcactttttt gatatgcctt
     1021 gtcacgttgc tcatcttctc cgcagagaat caagctgcgt ttatgactcc agttgcaagt
     1081 cttgccacaa gttttcaggt cgttctcctg atctacacag tcattccact gccactctat
     1141 ttatgtatac tcatcggaat aatctactca atcctatttg agattctcaa caagaacaaa
     1201 attggtcttg aagaggccgg atacattaag cttgtccttc acgctggtgt ccatttactc
     1261 ggtgtccatt tgttcatttt aactcaagtg cgacagagga aaacgtttct gaaagtggga
     1321 caatcgatgt tggcaagaaa ggatctagag ttggagacac agtttaaaga tcatatgatt
     1381 cagtctgtga tgtctaaaaa ggtagctgat gaattactga aagatgcatc agaacttcgc
     1441 cgaccttctg cttccaacga ctcaaactgc cgaacttcaa atgctacaca agttgaccag
     1501 ccccttgcca agatggtacc tgaataccga aagttccgtc catttacgat gaatctcatg
     1561 acaaatgtgt cgattctgtt tgcggatatt gctgggttta ctaaaatgtc atcaaataaa
     1621 tctgccgacg aacttgtcaa tctgctcaat gatctttttg gaagattcga tactttgtgt
     1681 cgattgagag gattagagaa gatttcaact ttgggagatt gttactattg tgttgctgga
     1741 tgtcctgaac catgtgatga tcatgcatgc agaacagtcg aaatgggtct cgacatgatt
     1801 gttgcaatcc gccaatttga tattgatcgt ggacaagaag tgaatatgcg tgtgggaatt
     1861 catacaggaa aagtaatgtg cggaatggtt ggaacaaaaa gattcaaatt cgatgtgttc
     1921 tcaaacgatg tcacactggc aaatgagatg gaatcttctg gtgttgctgg aagagttcac
     1981 gtttccgaag caactgcaaa acttttgaaa gggctttatg aaattgaaga gggaccggat
     2041 tatgatggtc cattgaggat gcaagttcag ggaactgaac gtcgtgttaa accagaaagc
     2101 atgaagactt tctttatcaa aggacgtatt aatgatggtg tagaagaaga agtgatgcaa
     2161 gttcaggaag ttgagagcct tcacagtcag aaaagcagta aaaagtcgac tctaaaacag
     2221 aaatgggcgg aaaagttgaa aatgaaccac acaaattctt atccaatgcg agccgctgca
     2281 cgtgaaggag gtggaagttt gagaataaaa ttggcagaga gaaatagaag tacacagtta
     2341 ctgccaaaag aatcaaattc aatttgtata atggaagata accgaaagtc agcatcactt
     2401 caagcattgg caacgaataa ttttaatgga agtaatacag atacaaataa cacttatagt
     2461 gaacgtggag ttgctggaag tgttagtaag aagtctgtag ctggaagtga aagtaatagt
     2521 ataaaaggaa gtagaagcag tggactacaa ttatctctac aagatggaaa ttctgattta
     2581 aattcagttg gaggtttgga tactgcaatc agtcaccatc acaatgcagc atctctcact
     2641 cgatttgata ctgataacaa ttttgatcag cgtttggcaa tggtaattgg acaaggagaa
     2701 ggaggatttg ataaaggatt ttggaatcat catgatagtt tgaataagtg gactcttcga
     2761 tttaatgaaa aggacgttga agaggaatat cgagctcatt ttgtggattc atcggaaaga
     2821 tatacggcat caaagaaagg tcatgttgaa agacataaag atttgatgga acaaggtgga
     2881 gaaaaagatg gaattactgg atcaacggtc aacaagtata gatattcagg agtcttcatc
     2941 gatataattg ttgccacgtt gatatttgta atatcgggtg cagttgcaat tatgtctgtc
     3001 cgtccattcc cattatcact cattgcatac ttcccatttg ctgctgcaat tctcattctt
     3061 acaattgtcc ttattggtct tccacttctc gcccgtaaaa aatcattcca atgtgcaaat
     3121 caatggatgc cacgtcatct catcggtctt cttctgatct ttcttccaat cggtgtcgca
     3181 atctgtatca tgccattgtg ccaaagtgga gactgtgcaa atgtaattct gaactaccgt
     3241 cttgcattca gttatgtcac cattcttgcc atattcgctc attgcaactt ctcccaatta
     3301 gctgcctggc caaaaactac tgctgcagta tttatcggac ttctccacat tgctggagtt
     3361 ttctactgcg aattcaattt gaaacactta gttgaagagc aagacacctg caacgtgaca
     3421 gcaatcatga ttccaccaat tcgcaaaggt ctgaactaca caattgcatt gaactcgaca
     3481 tctgcaagaa cattatctca agattttgga tctccattat tcatttggga gctgcttctg
     3541 gatgtgatcc tgtcgattgt tctagttgca ttccttaatt atcagttcga aaccgcattc
     3601 cgtatgtcat tctttggaga tgttcaagct cgtcgggaca ctgaaagaat gcaaatcgtg
     3661 cgtgatcaag ccgattggct cctgaataac gtgatcccag cccacgcagt agaaagtctt
     3721 aaaacggata ccaaatacag tgaaaaccat gaaacagttg gtgtactgtt cgcttcaatc
     3781 actaattgga atgatatgta tgaagagaat ttcgaagggg gcagagaatt cttgagagtt
     3841 ctcaatgaag ttattggtga ctttgatgag cttctcgatc ggccggactt cacgcatatt
     3901 gaaaaaatca aaacaattgg gcctgcgtat atggcagcaa gtggattgaa tccagaacga
     3961 aaaaagaata tgttgcatcc aaaagagcat ctatatcaga tggttgattt tgcattggca
     4021 gttcaacatg tactcagtgt tttcaacgag gatttgctca attttgattt tgtttgcaag
     4081 ttaggactta atattggacc cgtcactgct ggtgtcattg gaacaacaaa gttgtactac
     4141 gatatctggg gtgacactgt aaacattgct agccgaatgt acagcaccgg agttttaaat
     4201 cgcattcaag tatcacaaca cacacgagaa tatctcctgg atcggtatga attcgagttc
     4261 cgcgatcata ttgaagtcaa gggaattgat ggaggaatgg atacttatct tttggttggt
     4321 cgaaaaggtg atggaatacc tccatcaatc aaggataacc aagaagacga attttaaatc
     4381 tcgaggagct cccgagatct tcagcgctca gcattcgtag aattgtaagt ttaaactttc
     4441 tcatactaac taacgcgctc tatttaaatt ttcagaattc caactgagcg ccggtcgcta
     4501 ccattaccaa cttgtctggt gtcaaaaata ataggggccg ctgtcatcag agtaagttta
     4561 aactgagttc tactaactaa cgagtaatat ttaaattttc agcatctcgc gcccgtgcct
     4621 ctgacttcta agtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc
     4681 accccctatt tttgttatta tcaaaaaaac ttcttcttaa tttctttgtt ttttagcttc
     4741 ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata
     4801 aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta
     4861 cacaatgttc tgtgtacact tcttatgttt tttttacttc tgataaattt tttttgaaac
     4921 atcatagaaa aaaccgcaca caaaatacct tatcatatgt tacgtttcag tttatgaccg
     4981 caatttttat ttcttcgcac gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa
     5041 aaagttttgg agtatttttg gaatttttca atcaagtgaa agtttatgaa attaattttc
     5101 ctgcttttgc tttttggggg tttcccctat tgtttgtcaa gagtttcgag gacggcgttt
     5161 ttcttgctaa aatcacaagt attgatgagc acgatgcaag aaagatcgga agaaggtttg
     5221 ggtttgaggc tcagtggaag gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta
     5281 tggggttttt gccttaaatg acagaataca ttcccaatat accaaacata actgtttcct
     5341 actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     5401 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     5461 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     5521 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     5581 gtaaggagaa aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata
     5641 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     5701 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5761 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5821 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5881 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5941 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6001 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6061 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6121 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6181 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6241 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6301 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6361 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6421 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6481 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6541 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6601 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6661 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6721 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6781 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6841 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6901 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6961 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     7021 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7081 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7141 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7201 caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag
     7261 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7321 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7381 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7441 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7501 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7561 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     7621 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     7681 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     7741 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     7801 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctgtaag tttaaacatg
     7861 atcttactaa ctaactattc tcatttaaat tttcagagct taaaaatggc tgaaatcact
     7921 cacaacgatg gatacgctaa caacttggaa atgaaat
//
