LOCUS       sequence-208488-        8694 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the C. elegans unc-31 cDNA pan-neuronally.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Charlie NK, Schade MA, Thomure AM, Miller KG
  TITLE     Presynaptic UNC-31 (CAPS) is required to activate the G alpha(s) 
            pathway of the Caenorhabditis elegans synaptic signaling network.
  JOURNAL   Genetics. 2006 Feb;172(2):943-61. doi: 10.1534/genetics.105.049577. 
            Epub 2005 Nov 4.
  PUBMED    16272411
REFERENCE   2  (bases 1 to 8694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.105.049577"; journalName: "Genetics"; date: 
            "2006-02"; volume: "172"; issue: "2"; pages: "943-61"
COMMENT     Sequence Label: KG#121
FEATURES             Location/Qualifiers
     source          1..8694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6134..6156)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6256..6275
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6353..6371)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6439..6543
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6544..6612,6613..7404)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6762..6781)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7575..8163
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8064..8083
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8317..8334
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8451..8472
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8487..8492,8493..8510,8511..8517)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8525..8541
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8530..8552
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8549..8565
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8549..8565
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcaggatct tcagatggga gcagtggact gtctatttga tattcttaaa
       61 atgtactcta taaaagatga aactgtaatg accaattccc tacataaata tttttaacta
      121 attggtcgga tatttgggga tcagagaagt acttgttttt gcgatattaa taatgatata
      181 tttttctagt aaaaaaatgt taagcgattt gtgaataatt aagttggctt gaaaaagatt
      241 ttttgatagc tatgtatttt gttaatttaa atcagatttt aaaactttat tattgactat
      301 ttatatatat tatatattat atattttcat ggtttaatca aaatctatta aattatttct
      361 aaaatctcta aatatcgaat tctctaaaaa gcttcatcaa caaaagcatt tcaattatct
      421 aacacaatga gaataattca agaactgaaa tagaaagaaa tctagaaaaa aaagagagtg
      481 aggtgcccta ttaaatcggt gctctcctct ctaacagaag cgcctacacg cacccaagag
      541 agctcttttt ctttcttttt tcggagcaca catacacgca tacactcctc agaagtgtat
      601 agagaaaatg ggagaagaag cgaaagaaga cgaagaagcg gttgtgatgt atgcatcggg
      661 cgtttctatc tctctccgtg agcaacgagc tagtcaaccc atcgatccat ttttgtgagc
      721 acacacagag agagactcaa acacactcac agcttcacac tgagctccaa gagctctgta
      781 gagagaagcc agagtatgct ttcctcgatt ttcctaacgt cttcttcttc tgcttttttc
      841 tttttcccaa cgagttgtag tccactaact tcaatttttc ctccagagaa agagttttag
      901 agagacagct gataaataca ctagagaaat aaaaagaatg ataaaaattt gctattttaa
      961 tcagttccct ctcgtttctc taagttttgt tgttgggttg ggaaattaaa attggaaaat
     1021 ggaaataacg tttttttctg tctaatcttg aattctccag acatttctta tttccttttt
     1081 tgttgcaagt ttttcttcct tctctaattt ttaatcaaag atatctcgct atgaactttg
     1141 aatttttgat attttgattc tataaataca taaagtaatc taaatgctct tttaaaataa
     1201 atctacagta gccctatttt caggatcccc gggattggcc aaaggaccca aaggtatgtt
     1261 tcgaatgata ctaacataac atagaacatt ttcaggagga cccttggcta gcaaaaatgt
     1321 cgaatgtttc aaagccgata atgcaaaatt cacaagaatt cgatggagat gaggaagaag
     1381 gggaagaagg ttcaactaca gtagtcggta gtggagatga atgtggtggt cctgctttga
     1441 gcaaagaaga gcaggaaaga atgaaagccg aacgagagga agaagatcat aagaagaatc
     1501 ttcaaatgta catgtttcta gccagatgca tagcatatcc attcaatgga cagcagactg
     1561 gtgacatggc tcgtcgacaa atgaaagtga ataaacagga attggctaga attcgagaac
     1621 gattcactct ttttcttaaa ggagaaacaa atattgctgc tgatgaagca ttcactaaag
     1681 cgattcaaag ttatttcgaa gtatttctga aatctgaaag agttcagaaa gttgttcacg
     1741 ccggcggatt ttcacaacat gattttcgag aagtttttcg attaaatatt gagaaaaggg
     1801 ttcgaagtct tcctgatatt gaaggattat caaaggatac tgtattgaat tcttggttag
     1861 caaaattcga tgcaattatt aaaggagatg agactgatca gaataggaat gctcgaggcc
     1921 gctctcgaaa tccgcagaat gcgatgagcg ctgacgcagt actaggcaaa gagcaactct
     1981 acgacgtttt tcaacaaatt cttggtgtga agaaattcga gcatcaaatc atattcaacg
     2041 cacttcagtt ggataatcca gatgagcagg cggcagcaat ccggagagag tttgcgacaa
     2101 gagaagaagc tttgaaggat cctatcaaga tgaagcgtct tactccaaaa tttgtggtaa
     2161 aagacatgga aaccctgtac atggacgaag ttcgaatgtc gataaacact ttaataggaa
     2221 atttggaaac agttccagtg acaacacgag gggcaactgt cggaaaacga aaagacaaga
     2281 gtcgatcccg cagtatagag gatttgtcgc tcttcaacag ccttaaacga aggacaagct
     2341 ccgggagtct caacaaagga gatagtgaag atggtgacgt cacactgaca aagtctgatg
     2401 tctcattggc tttgagtatg gaggttgtcg tgatggaagt gcagggtcta aaatccgtgc
     2461 aacccagtaa aatagtgtat tgcacgatgg aagtagacgg tcacaagctg cagacagatc
     2521 atgcggaggc ttccaaacca aaatgggata cacaaggaga cttttcgacg aaaaatccat
     2581 tgcccgtggt gaaagtgaaa ttgtatacag aagtgaagag tatgatagca tttgaggata
     2641 aagaattagg aaaagtaata atccagccga cgccaaactg tgcgcgaagt ccagaatggt
     2701 acaccatgac attaccaaaa agtagtcagg atcagaattt aaagattagg atagcaattc
     2761 gagtagaaaa accgccgaat ttaaagtatt gcggttattg ttattgtatc gggcgaaacg
     2821 cgtggaagaa gtggaagaag cgattctttt gtcttgttca agtatctcag tacgcatttg
     2881 ctgtatgtag ttttcgacaa aagaaggccg atcccacgga attcattcaa ttagacgggt
     2941 ttacgatcga ttatatgccg gagtcggatc cagaactctc tgcgcaagga ggaaaacact
     3001 tcttcactgc catcaaagaa ggagacgagc tgaaatttgc gactgatgac gaaaatgagc
     3061 gacatctatg ggtccaggct ctgtaccgag ccacaggtca agcctataaa cctgtgcctc
     3121 caaagcagtc aacaattgct ccaaaggctc aaggtttcca ggataaagcc agcaaacatg
     3181 gaatggatgc aatgattcag gctgattcga ttaatttcga tcatgaccat ttttattccg
     3241 atgttcaacg attaactctg gattttcgga tcaacgagcc gatttgttca ttgggttggt
     3301 tctcacctgg tcaagcattt gttctggaag aatactctgc tcgatatatg gttcgtggat
     3361 gcttccgcca cgtaaccctc ttatcaaatc ttttggataa agctgacgat ggattgctca
     3421 ttgatccagc tctaattcat tactctttcg ctttttgtgc atctcatgtc catggaaata
     3481 ggtgtatgcc tgatcgacaa ggacctgaag gagttggtac agtaactctg gaggagaagg
     3541 aaaagttcca ggaaatcaag gaacgattga gagtacttct agagaagcaa atcacgaatt
     3601 tccgttattg tttcccattt ggaagacctg aaggcgcact taaagggaca ttaggtctat
     3661 tggaaagagt tttgatgaag gatgttgtgt cgccagttcc accggaagaa gttcgagcag
     3721 ttattagaaa atgtttggag gatgctgcat tggttaatta cacaaggatt tgcaatgaag
     3781 caaagattga acaacgaatg ggtattgatg ttagtccggc tcaacgaatc gaggacatga
     3841 tccgcgtgac ggaattctgc attgatctgc tcaaggagaa cgaggagcat catggagagg
     3901 ctttcgcgtg gttttccgac cttctttccg accattccga gatcttttgg tcattgtatt
     3961 cagtcgactt ggactcggca ctggaagttc aaccacacga tagttgggat tctttccctc
     4021 ttttccagat gctcaacgac tttttgctgt ctgaatcaag cctgaaagga ggtatattcc
     4081 acaacaaaat cgttcaacaa ttccaacctc tcgttgtacg atacattgac ctcatggagc
     4141 actcgattgc tcaggcgatt gacaaaggat tttccaagga aaaatgggaa tcaagaaaag
     4201 aaggatgtgc aacttcggaa gatatttact ggaagctcga cgctcttcac acatttgtca
     4261 tcgatctgaa ctggcctgaa gaggatttca gaaaatattt gcagacgaaa atgaaaagtc
     4321 taacttctga tatgatatcc aaggtttccg attgcacctt caccgcattc gacagttgga
     4381 tgcaacgagc caaaaagtcc accgattaca tgcttccatc agaagtttgc gtgcaaatca
     4441 acgtaatgtt cagctcaaag agccgtgctg ttcgagtcac tgttgattct ggagaataca
     4501 aatatcagtc aaaactcgac gaaactctgg agacaatgct caaaactatg gaatcatgta
     4561 ttcaagagaa acttcacgga gttcttgaat cagtgctctc acgtcttgct cgatacgacg
     4621 agggaaatcc gattggagca attttgaata ttgccccaaa gcctgcatcg attttcaaca
     4681 aattgaagac aatggccgga gacacctcgg ctcaggcaac gacgacagcg cgtcaacctc
     4741 tgactgctca acaatcatca gggcaaatcg gaaattcata tgttacattt ttccacggat
     4801 gcactgaact tcttcgacaa gttattatcg atgaaatttg ggttaatggg ctcttcgagc
     4861 attggtacga caaccaaatg aaatcaatca atgaatggtt gacagaacgt cttcaacaat
     4921 cgttgagtgc aactcaatac atttcccttt cgacaattgt aaaaaaagtg tatcaagact
     4981 tttcactgca aggaatcgat gaagaacgac tgaatagtaa gacttatcag tcaatcagtc
     5041 gacgattaca gcttgaagag tcaaattctc atattcaaga aggtatacga aaacattaaa
     5101 taccggtcag atatcactcg aggagctccc gagatcttca gcgctcagca ttcgtagaat
     5161 tgtaagttta aactttctca tactaactaa cgcgctctat ttaaattttc agaattccaa
     5221 ctgagcgccg gtcgctacca ttaccaactt gtctggtgtc aaaaataata ggggccgctg
     5281 tcatcagagt aagtttaaac tgagttctac taactaacga gtaatattta aattttcagc
     5341 atctcgcgcc cgtgcctctg acttctaagt ccaattactc ttcaacatcc ctacatgctc
     5401 tttctccctg tgctcccacc ccctattttt gttattatca aaaaaacttc ttcttaattt
     5461 ctttgttttt tagcttcttt taagtcacct ctaacaatga aattgtgtag attcaaaaat
     5521 agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca ttaataataa
     5581 ttctatccca aaatctacac aatgttctgt gtacacttct tatgtttttt ttacttctga
     5641 taaatttttt ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac
     5701 gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa
     5761 atcatgctca tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt
     5821 ttatgaaatt aattttcctg cttttgcttt ttgggggttt cccctattgt ttgtcaagag
     5881 tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa
     5941 gatcggaaga aggtttgggt ttgaggctca gtggaaggtg agtagaagtt gataatttga
     6001 aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc ccaatatacc
     6061 aaacataact gtttcctact agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg
     6121 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     6181 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     6241 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     6301 aaataccgca cagatgcgta aggagaaaat accgcatcag gcggccttaa gggcctcgtg
     6361 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     6421 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     6481 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     6541 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     6601 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     6661 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6721 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6781 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6841 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6901 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6961 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7021 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7081 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7141 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     7201 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     7261 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     7321 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     7381 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     7441 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     7501 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7561 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7621 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7681 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     7741 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7801 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7861 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7921 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc
     7981 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8041 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8101 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8161 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8221 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     8281 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     8341 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     8401 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     8461 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     8521 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     8581 ctgtaagttt aaacatgatc ttactaacta actattctca tttaaatttt cagagcttaa
     8641 aaatggctga aatcactcac aacgatggat acgctaacaa cttggaaatg aaat
//
