LOCUS       sequence-208504-       11287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A bicistronic lentiviral mammalian expression vector with a multiple
            cloning site and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11287)
  AUTHORS   Zhang K, Myllymaki SM, Gao P, Devarajan R, Kytola V, Nykter M, Wei 
            GH, Manninen A
  TITLE     Oncogenic K-Ras upregulates ITGA6 expression via FOSL1 to induce 
            anoikis resistance and synergizes with alphaV-Class integrins to 
            promote EMT.
  JOURNAL   Oncogene. 2017 Oct 12;36(41):5681-5694. doi: 10.1038/onc.2017.177. 
            Epub 2017 Jun 12.
  PUBMED    28604746
REFERENCE   2  (bases 1 to 11287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/onc.2017.177"; journalName: "Oncogene"; date: "2017-10-12- 
            12"; volume: "36"; issue: "41"; pages: "5681-5694"
COMMENT     Sequence Label: pLVET-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..11287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2145..3323
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2376..3314
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3271..3291
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3371..3488
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3551..4117
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3567..4119
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3711..3728)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3938..3957
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4131..4133,4134..4136,4137..4850)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4176..4197)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4437..4456)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4784..4805
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4868..5456
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4921..4941)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5339..5350)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(5594..5612,5613..5635,5636..5654,5655..5677,5678..5696,
                     5697..5719,5720..5738,5739..5761,5762..5780,5781..5803,
                     5804..5822,5823..5845,5846..5864)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     LTR             5903..6083
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6108..6126)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6109..6126)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6416..6435)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6649..6668)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6768..6790
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6828..6846)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6914..7018
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7019..7087,7088..7879)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7237..7256)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8050..8638
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8539..8558
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8792..8809
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8879..8899)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8884..9213
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9064..9199
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10347..10412
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10542..10562
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10987..11121
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11024..11043)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11078..11097
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgatg tcgacgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc
     2101 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
     2161 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2221 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2281 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2341 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     2401 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     2461 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     2521 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     2581 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     2641 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     2701 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     2761 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     2821 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     2881 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     2941 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     3001 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     3061 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     3121 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     3181 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     3241 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3301 ttcttccatt tcaggtgtcg tgaggaattt cgacatttaa atttaattaa tctcgacggt
     3361 atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3421 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3481 aaaattttat cgatcacgag actagcctcg aggtttaaac tacgggatcc aggcctaagc
     3541 ttacgcgaat tccccccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     3601 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     3661 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     3721 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     3781 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     3841 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     3901 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     3961 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     4021 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     4081 cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaacc atggtgagca
     4141 agggtgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     4201 acggccacaa gttcagcgtg tctggcgagg gcgagggcga tgccacctac ggcaagctga
     4261 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     4321 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     4381 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     4441 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     4501 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     4561 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     4621 cgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     4681 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     4741 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     4801 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa gggactagtc
     4861 atatgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     4921 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     4981 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     5041 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     5101 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     5161 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     5221 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     5281 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     5341 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     5401 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     5461 gtacgtatgg ccaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     5521 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     5581 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     5641 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     5701 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     5761 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     5821 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcggat
     5881 cgatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     5941 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6001 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6061 gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac
     6121 ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac
     6181 aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc
     6241 tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc
     6301 gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc
     6361 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
     6421 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
     6481 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
     6541 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
     6601 ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc
     6661 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     6721 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     6781 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     6841 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     6901 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6961 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     7021 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     7081 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     7141 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     7201 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     7261 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     7321 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     7381 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     7441 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7501 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7561 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7621 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7681 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7741 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7801 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7861 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7921 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7981 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8041 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     8101 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     8161 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     8221 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     8281 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8341 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8401 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8461 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8521 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8581 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8641 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8701 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8761 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     8821 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgtgg
     8881 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     8941 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     9001 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     9061 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9121 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     9181 ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac aggcttgcga
     9241 gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc
     9301 ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg cgcaacctat
     9361 tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc acatgagcga
     9421 aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact
     9481 gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt
     9541 gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac
     9601 gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc
     9661 ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg
     9721 tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat
     9781 gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga tatgggcgtc
     9841 gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact gccgggcgtt
     9901 gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg aggctgcatc
     9961 catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac
    10021 ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga
    10081 tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg
    10141 cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg
    10201 taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact ggatttatga
    10261 gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
    10321 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
    10381 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
    10441 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
    10501 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
    10561 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
    10621 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
    10681 acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
    10741 ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
    10801 ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
    10861 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
    10921 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
    10981 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    11041 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    11101 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
    11161 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
    11221 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
    11281 ttagtag
//
