LOCUS       sequence-208505-        8562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the active zone - enriched protein Sentryn-GFP in a subset
            of cholinergic ventral nerve cord motor neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8562)
  AUTHORS   Edwards SL, Morrison LM, Manning L, Stec N, Richmond JE, Miller KG
  TITLE     Sentryn Acts with a Subset of Active Zone Proteins To Optimize the 
            Localization of Synaptic Vesicles in Caenorhabditis elegans.
  JOURNAL   Genetics. 2018 Nov;210(3):947-968. doi: 10.1534/genetics.118.301466.
  PUBMED    30401765
REFERENCE   2  (bases 1 to 8562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.118"; journalName: "Genetics"; date: "2018-11"; 
            volume: "210"; issue: "3"; pages: "947-968"
COMMENT     Sequence Label: KG#797
FEATURES             Location/Qualifiers
     source          1..8562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4183..4211)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4959..4979
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(6008..6030)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6130..6149
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6227..6245)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6313..6417
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6418..6486,6487..7278)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6636..6655)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7449..8037
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7938..7957
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8191..8208
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8325..8346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8361..8366,8367..8384,8385..8391)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8399..8415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8404..8426
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8423..8439
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8423..8439
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tgaaataagc ttgggctgca gcccgggaaa catgatatcg acggacatat ttttgattga
       61 ttatcataat tttcgtggga atcttcggaa catgctcttt gcggtttata agtggagatg
      121 cgccaacggg aaacttgttt taaaactatt cctatggtgc taacatcacc acatgaaaac
      181 ttttcaaaac ttttttgggt tttttatttt tttattatta ttcgtatcta aacattgtag
      241 gcgttgtgat gttgccctaa atttctttaa aaagagcgaa cattttcaaa aatttgctat
      301 ttaaatcgaa tgaaaatttc cattcaaata tttgaaaaaa attatgaaaa aattcctttt
      361 gttgcatctt catcttctat gaaagttaca attttagttg gatcagaaat atgagatatc
      421 aaaaaaaaat taagattttt ttgtttgttt ttttgaagcc taaaatattt tgatatcatt
      481 tatctcttat gtcttttgtt ttatcgaaac gtttacaaca catcaactat gcgtcaataa
      541 attttttagg tagttaaaca aatgttgaaa aaaaaagcaa aagcaaccaa gagctgttaa
      601 aatagagaaa atgcagttat tttttatatt caagaaaagt tatcacagaa gttccgtaat
      661 gagttttttc cttatcaaca tgttcccatc aacttcttag tttctcctca ttcccattgt
      721 tcaacataat tatcacctct gaccttattc tgctcttttt aagcacaggt gttaatatgc
      781 cgtccatcat atttgccctc cgtctgccgc tttccttatc tgacctttac atcgcctttt
      841 tgtatatgta cataataatt gtatcatcat caacctccgc cataaacatg catgtctttt
      901 tacctctttt ggcatgtacc gttcttcttt gtatttttat tatgtcttcc ttccatcttc
      961 cctctttttt acggattttc ggattgcgga agaagaagat ggtcgataat taccgtaatg
     1021 aggtaatttg aaacatattg gacagaagaa atacgcgcgg atgctttcgg tggcaaggtt
     1081 aattcgttgt ctttgtttct ggaatgtcct tcataaaaaa cacggattac cacctgtttt
     1141 taacgtggat taagattttt ggaaacaatt tactctgcct aatttttttg tacatttaaa
     1201 aatgtagact taccgaattc accgagcttg accagtttta aaggttaccg ttcacactaa
     1261 aaattgttga aaaaattttc gactaaacct aatgatttta ttttaaaaaa taaatgaaat
     1321 agcatacaaa tttggatttt tttcaatttt tttcaagaaa atcatgaaac atttagaaaa
     1381 ctcgagtaac aagctttccg ttcattctgt aatttttaag ttccaaattt aaaaaaatgc
     1441 attttgattt actgggattg ccgtgctcgt aattatcagc aaaacatttg ccgaattagc
     1501 cgagctcgac aatctatttt ttcgcaaact tttgccatgt ttcgtaaaac aattttccat
     1561 tttatggtag acatttttca tcaatgaatt cacaaaattt aaaatcccca ctgaaagtac
     1621 caaaatcttc ctgaaaaatc cggcaaaaaa tccatctcat tatcatgtca catatacatg
     1681 ttctattggc actatgtttg ggatgtcctc caattaatac ttctgtgtca atctcatcgg
     1741 cgcccggcgg ccactaccaa atgcaaaact ttgtttgtaa aagaagaagc caataatttt
     1801 ctcgttattc atttttatta ctctttttgc gtttcattct tctttgttcg aattgatgaa
     1861 cgcacgttgc ttctctctgg ttttcctgcc gttccttata ttgccaagga aaccaatgcg
     1921 gattttcgga agattttctt catgtccttt agaaaatatc accacttttc ttctttttcc
     1981 atttcaaatt tcaatttcca tacaactcat cttctattat agcttttcag gtgttcgact
     2041 gtttgaagat aagaatcaat taaaatttgg ttcactgagc atgtcatcca cttttccttt
     2101 tctttgttct aaaattcacc aaactgaatt ttccttaacc ggaaatttac atgaaaataa
     2161 gaaacatgga ttacatcctt tacatatcat ttttgttttt ttatgtgtat tccatgttcc
     2221 caaaagtttg atgcaatttt tgattctttt tcaattttcc aaattcgtgt ctcgcaggga
     2281 acgattcaca gtccaacatt cccaaattgc gaaattttag aatatatatt cccaagcgtc
     2341 gaatatctca aaaatttgtc gttttctcag tttgacatgt cacctttgcc ggttcctcta
     2401 ttcgtctatt agctctccag agagcaatgt catgtcacac gaagaattca gggacatgtt
     2461 tcgttttttt ttgtttgcat gtggtcaccg atatgggaac ttgaactttc caatttttgc
     2521 ttgctttttc ttttccggaa acttaatact atttgagaaa ctccgcatct tatttgtttg
     2581 atcccaaatt ctgaaattcc tctttgaatt tgatctcaag cttcactata aaaaattgtt
     2641 tcaggaaaat tggatccccg ggattggcca aaggacccaa aggtatgttt cgaatgatac
     2701 taacataaca tagaacattt tcaggaggac ccttggctag caaaaatgat gaactccacc
     2761 tcaggatcat ccacaatacg agatttggtg acaccaaaga attgcaactg ggattttgaa
     2821 tcatttaacc aacaccatca agaaaagcac accagcaagc agcccgcgtg taattgtgtt
     2881 gctgaagcaa ttcgacgcgt cactgctctt cggaagttgg caacacagca agatggagat
     2941 tttacaatgg ttcaaaacaa tgaagtcaag tgggcaaaac tttttatgga attcatcatt
     3001 ggaactgcgt attgtcagtc tacaaagtgt caagcccact ccgacgatat gatttggtat
     3061 gttcaccagg cgaaactctc caattccttt tacattcaag ggaatcaaag cttgttgcaa
     3121 gtttttcgtc gacacagcaa aaaccaacca acgccttgcg atgacagcta caactgggag
     3181 gagactgttt gcctgaacat aatcatgcaa cagattgact tttatgttac ctgtgccgtt
     3241 tgcacaaaaa ctggacctca aaatttgcaa attattcgca aaaactgtca gcgtgtattc
     3301 ccatccccga gtcgtcgtcg tatggatagt aaaggagaga atgaagagat tacatatcct
     3361 aaattatatt ttgcaattga cggttttgaa gaggttttta acgacgtaat tgtgcgtgac
     3421 ggagagtgtg tatgtgttga actggttgca cgtgatcgcc agaaaactcg agagtccgtt
     3481 gtctttctcg gatcaatacg atatgaaatt ctgaaacaag tgtatgacac taaagcttct
     3541 tcgacgtggc agtgggcaca aaagttgatg aaagtaggcg atcgacgtca agaatttgta
     3601 cgaatgcgcg gtccgcaggg caaaggatat gcggaaatgg cggtggcaag agtgcccaac
     3661 tgcggatacg aaacgccgct caccgaaaat cctattgact tttctgagtt ggcaggagta
     3721 gaacagtcac ttggaaaacg tcgaatgtca gagacaaatc ttgccggcgg aacacggatg
     3781 ttcccttctc gttcaattgc cactccatct cattcatcgc ggggaagacg ttggcaaagt
     3841 gaggcggaca ctgtgaacca ataccccgaa gttgagggaa gcaatatcga cgacgaactt
     3901 gacgaaggcc caacgaacaa gctctggtcg atgcgaggtt ttgcgcaagt ttggcatgga
     3961 cttcgagaga agaaacgcgc agagtgcaca ccactgaatg catttttgac ctacgtcaca
     4021 ctgccctgga acagtatttt aaatgatctt ctgagtgaac gacaaaaacg tccgattttg
     4081 acgttcgagt ttgatgcacc agctcataat cacgattctt ccggatcctc tggagcggta
     4141 ccggtagaaa aaatgagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
     4201 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
     4261 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
     4321 tgggtaagtt taaacatata tatactaact aaccctgatt atttaaattt tcagccaaca
     4381 cttgtcacta ctttctgtta tggtgttcaa tgcttctcga gatacccaga tcatatgaaa
     4441 cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
     4501 ttcaaagatg acgggaacta caagacacgt aagtttaaac agttcggtac taactaacca
     4561 tacatattta aattttcagg tgctgaagtc aagtttgaag gtgataccct tgttaataga
     4621 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
     4681 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
     4741 gttgtaagtt taaacatgat tttactaact aactaatctg atttaaattt tcagaacttc
     4801 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
     4861 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
     4921 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca
     4981 gctgctggga ttacacatgg catggatgaa ctatacaaat agcattcgta gaattgtaag
     5041 tttaaacttt ctcatactaa ctaacgcgct ctatttaaat tttcagaatt ccaactgagc
     5101 gccggtcgct accattacca acttgtctgg tgtcaaaaat aataggggcc gctgtcatca
     5161 gagtaagttt aaactgagtt ctactaacta acgagtaata tttaaatttt cagcatctcg
     5221 cgcccgtgcc tctgacttct aagtccaatt actcttcaac atccctacat gctctttctc
     5281 cctgtgctcc caccccctat ttttgttatt atcaaaaaaa cttcttctta atttctttgt
     5341 tttttagctt cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt
     5401 aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat
     5461 cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttttactt ctgataaatt
     5521 ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca
     5581 gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg
     5641 ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga
     5701 aattaatttt cctgcttttg ctttttgggg gtttccccta ttgtttgtca agagtttcga
     5761 ggacggcgtt tttcttgcta aaatcacaag tattgatgag cacgatgcaa gaaagatcgg
     5821 aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga agttgataat ttgaaagtgg
     5881 agtagtgtct atggggtttt tgccttaaat gacagaatac attcccaata taccaaacat
     5941 aactgtttcc tactagtcgg ccgtacgggc cctttcgtct cgcgcgtttc ggtgatgacg
     6001 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     6061 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     6121 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     6181 cgcacagatg cgtaaggaga aaataccgca tcaggcggcc ttaagggcct cgtgatacgc
     6241 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     6301 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     6361 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     6421 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     6481 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     6541 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     6601 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     6661 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     6721 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     6781 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     6841 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     6901 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     6961 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     7021 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     7081 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     7141 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     7201 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     7261 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     7321 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     7381 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7441 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7501 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     7561 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     7621 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7681 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7741 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7801 cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt
     7861 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7921 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7981 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8041 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     8101 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     8161 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     8221 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     8281 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     8341 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     8401 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctgtaa
     8461 gtttaaacat gatcttacta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg
     8521 ctgaaatcac tcacaacgat ggatacgcta acaacttgga aa
//
