LOCUS       sequence-208511-        2574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  G4S-PdX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Iwata F, Hirakawa H, Nagamune T
  TITLE     A Stable Artificial Multienzymatic Complex Using a Heterotrimeric 
            Protein From Metallosphaera sedula.
  JOURNAL   Biotechnol J. 2018 Nov;13(11):e1700662. doi: 10.1002/biot.201700662.
            Epub 2018 Apr 30.
  PUBMED    29663675
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J."; date: "2018-11"; volume: "13(11):e1700662. doi"; pages: " 
            10.1002/biot.201700662. Epub 2018 Apr 30"
COMMENT     Sequence Label: pBsaG4SPdX
FEATURES             Location/Qualifiers
     source          1..2574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..31)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    39..55
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     44..66
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     63..79
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     63..79
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(495..512)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(495..511)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(504..526)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(666..683)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(837..1425)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(917..936)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1568..1670
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1671..2330
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1737..1756)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    2539..2560
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
       61 cacaggaaac agctatgacc atgattacga attggtctcc ggcggaggtg gctctatgtc
      121 taaagttgtt tacgtttctc acgacggtac ccgtcgcgaa ctggacgttg cggacggtgt
      181 ttctctgatg caggcggcag tttctaacgg catctacgac atcgttggtg actgcggtgg
      241 ttctgcgtct tgcgcgacct gccacgtgta cgtgaacgaa gcgttcaccg acaaagttcc
      301 ggctgctaac gaacgtgaaa tcggtatgct ggaatctgtt accgcggaac tgaaaccgaa
      361 ctctcgtctg tcttgccaga tcatcatgac cccggagctg gacggtatcg ttgttgacgt
      421 tccggaccgt cagtggtgag aattcgatcc gagaccgatc ctctagagtc gacctgcagg
      481 catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
      541 acccaactta atcgccttgc agcacatccc cctttcgcca gctggtgtaa tagcgaagag
      601 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg agcttcttcc
      661 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      721 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      781 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      841 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      901 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      961 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1021 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1081 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1141 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1201 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1261 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1321 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1381 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1441 ttttctacgg ggtctgacgc tcagtggaac tccgtcgaac ggaagatcac ttcgcagaat
     1501 aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt tggcgaaaat
     1561 gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata agatcactac
     1621 cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa
     1681 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
     1741 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
     1801 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
     1861 cccgcctgat gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga
     1921 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
     1981 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
     2041 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
     2101 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
     2161 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
     2221 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa
     2281 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag
     2341 gcagttattg gtgcccttaa acgcctggtt gctacgcctg aataagtgat aataagcgga
     2401 tgaatggcag aaattcagct tggcccagtg ccaagctcca atacgcaaac cgcctctccc
     2461 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     2521 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggc
//
