LOCUS       sequence-208519-       16024 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vectors used for the inducible expression of dual 
            intein-coupled tripartite split-sfGFP components in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16024)
  AUTHORS   Liu TY, Chou WC, Chen WY, Chu CY, Dai CY, Wu PY
  TITLE     Detection of membrane protein-protein interaction in planta based on
            dual intein-coupled tripartite split-GFP association.
  JOURNAL   Plant J. 2018 Feb 16. doi: 10.1111/tpj.13874.
  PUBMED    29451720
REFERENCE   2  (bases 1 to 16024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2018 Feb 16. doi: 10.1111/tpj.13874."
COMMENT     Sequence Label: UBQ10:sXVE:(MCS)-S11-DI-GFP1–9
FEATURES             Location/Qualifiers
     source          1..16024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..76
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      1834..2086
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2253..2277
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2742..3392
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(3580..3597)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3831..3850)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            4073..4182
                     /note="incP origin of transfer"
     CDS             4215..4586
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      4713..5424
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  6046..6813
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             7005..7799
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             8098..9246
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    10842..10866
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(11113..11134)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11325..11344
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11469..11491
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11483..11500
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11484..11500
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(11513..11713)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     CDS             complement(11760..12563)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGAWIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11871..11890)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     12481..12500
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(12613..12792)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 catgtaatat gctcgactct aggatcttcg caagaccctt cctctatata aggaagttca
       61 tttcatttgg agaggacacg ctgaagctag tcgactctag ccaaatctag gggatccggc
      121 gcgcccgggt taattaactc gagtctagtg gtggagggtc tggtggcgga tcaaccagtg
      181 aaaagcgtga ccacatggtc cttcttgagt atgtaactgc tgctgggatt acagatgcta
      241 gctgtttgtc cttcggaact gagatactta cagttgaata tggaccactt cctattggaa
      301 agattgtgag tgaagagatc aactgtagtg tttattccgt ggacccagag ggtagagttt
      361 acactcaagc tattgctcag tggcatgata ggggagaaca ggaggttctt gaatatgagt
      421 tggaagatgg ttctgtgata agagctacat cagatcacag gtttcttact acagattacc
      481 aacttttggc aatcgaagag attttcgcta gacagctcga tcttctcact ttggaaaata
      541 ttaagcaaac agaagaggca cttgataacc ataggcttcc atttcctctt ttggatgctg
      601 gaactattaa gatggttaaa gtgataggaa gaaggtcatt gggtgttcaa agaatatttg
      661 acatcggact tcctcaggat cacaatttct tactcgcaaa cggtgctatt gcagcagctt
      721 gtttcaatgg ttctggttca agagagtctg gagctatctc tggcgatagt ctgatcagcc
      781 tggcaagcac aggaaaaaga gtttctatta aagatttgtt agatgaaaaa gattttgaaa
      841 tatgggcaat taatgaacag acgatgaagc tagaatcagc taaagttagt cgtgtatttt
      901 gtactggcaa aaagctagtt tatattctaa aaactcgact aggtagaact atcaaggcaa
      961 cagcaaatca tagattttta actattgatg gttggaaaag attagatgag ctatctttaa
     1021 aagagcatat tgctctaccc cgtaaactag aaagctcctc tttacaatta tcaccagaaa
     1081 tagaaaagtt gtctcagagt gatatttact gggactccat cgtttctatt acggagactg
     1141 gagtcgaaga ggtttttgat ttgactgtgc caggaccaca taactttgtc gcgaatgaca
     1201 tcattgtaca caacagccga gggcccatga gaaaaggaga agaacttttc actggagttg
     1261 tcccaattct tattgaatta gatggtgatg ttaatgggca caaatttttt gtccgtggag
     1321 agggtgaagg tgatgcaaca atcggaaaac ttagccttaa atttatttgc actactggaa
     1381 aactacctgt tccatggcca acacttgtca ctacccttac ctatggtgtt caatgctttt
     1441 caagataccc agatcatatg aagcggcacg acttcttcaa gagcgccatg cctgagggat
     1501 acgtgcagga gaggaccatc tatttcaagg acgacgggac ttacaagaca cgtgctgaag
     1561 tcaagtttga gggagacacc ctcgtcaaca ggatcgagct taagggaatt gatttcaagg
     1621 aggacggaaa catcctcggc cacaagttgg aatacaactt taactcccac aaagtataca
     1681 tcacggccga caagcaaaat aacggcatca aagccaactt cacaattcgc cacaacgttg
     1741 aagacggctc tgtgcaactc gctgatcatt atcaacaaaa tactccaatt ggcgatggcc
     1801 ctgtcctttt accataagag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt
     1861 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     1921 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     1981 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     2041 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga attcagatcg
     2101 gctgagtggc tccttcaacg ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc
     2161 gcgtcatcgg cgggggtcat aacgtgactc ccttaattct ccgctcatga tcagattgtc
     2221 gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta
     2281 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc
     2341 gttcgtccat ttgtatgtgc atgccaacca cagggttccc cagatctggc gccggccagc
     2401 gagacgagca agattggccg ccgcccgaaa cgatccgaca gcgcgcccag cacaggtgcg
     2461 caggcaaatt gcaccaacgc atacagcgcc agcagaatgc catagtgggc ggtgacgtcg
     2521 ttcgagtgaa ccagatcgcg caggaggccc ggcagcaccg gcataatcag gccgatgccg
     2581 acagcgtcga gcgcgacagt gctcagaatt acgatcaggg gtatgttggg tttcacgtct
     2641 ggcctccgga ccagcctccg ctggtccgat tgaacgcgcg gattctttat cactgataag
     2701 ttggtggaca tattatgttt atcagtgata aagtgtcaag catgacaaag ttgcagccga
     2761 atacagtgat ccgtgccgcc ctggacctgt tgaacgaggt cggcgtagac ggtctgacga
     2821 cacgcaaact ggcggaacgg ttgggggttc agcagccggc gctttactgg cacttcagga
     2881 acaagcgggc gctgctcgac gcactggccg aagccatgct ggcggagaat catacgcatt
     2941 cggtgccgag agccgacgac gactggcgct catttctgat cgggaatgcc cgcagcttca
     3001 ggcaggcgct gctcgcctac cgcgatggcg cgcgcatcca tgccggcacg cgaccgggcg
     3061 caccgcagat ggaaacggcc gacgcgcagc ttcgcttcct ctgcgaggcg ggtttttcgg
     3121 ccggggacgc cgtcaatgcg ctgatgacaa tcagctactt cactgttggg gccgtgcttg
     3181 aggagcaggc cggcgacagc gatgccggcg agcgcggcgg caccgttgaa caggctccgc
     3241 tctcgccgct gttgcgggcc gcgatagacg ccttcgacga agccggtccg gacgcagcgt
     3301 tcgagcaggg actcgcggtg attgtcgatg gattggcgaa aaggaggctc gttgtcagga
     3361 acgttgaagg accgagaaag ggtgacgatt gatcaggacc gctgccggag cgcaacccac
     3421 tcactacagc agagccatgt agacaacatc ccctccccct ttccaccgcg tcagacgccc
     3481 gtagcagccc gctacgggct ttttcatgcc ctgccctagc gtccaagcct cacggccgcg
     3541 ctcggcctct ctggcggcct tctggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3601 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3661 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3721 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3781 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3841 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3901 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt ttccgctgca taaccctgct
     3961 tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat
     4021 tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga
     4081 taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc
     4141 tggcggtgct caacgggaat cctgctctgc gaggctggcc ggctaccgcc ggcgtaacag
     4201 atgagggcaa gcggatggct gatgaaacca agccaaccag gaagggcagc ccacctatca
     4261 aggtgtactg ccttccagac gaacgaagag cgattgagga aaaggcggcg gcggccggca
     4321 tgagcctgtc ggcctacctg ctggccgtcg gccagggcta caaaatcacg ggcgtcgtgg
     4381 actatgagca cgtccgcgag ctggcccgca tcaatggcga cctgggccgc ctgggcggcc
     4441 tgctgaaact ctggctcacc gacgacccgc gcacggcgcg gttcggtgat gccacgatcc
     4501 tcgccctgct ggcgaagatc gaagagaagc aggacgagct tggcaaggtc atgatgggcg
     4561 tggtccgccc gagggcagag ccatgacttt tttagccgct aaaacggccg gggggtgcgc
     4621 gtgattgcca agcacgtccc catgcgctcc atcaagaaga gcgacttcgc ggagctggtg
     4681 aagtacatca ccgacgagca aggcaagacc gagcgccttt ccgacgctca ccgggctggt
     4741 tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag aaacgccgtc
     4801 gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg aaaacttggc
     4861 cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac ccggcgcggc
     4921 gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc cagcctcgca
     4981 aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga caagcctggg
     5041 gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat gaggggcgcg
     5101 atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat tgacatttga
     5161 ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt ttttcggcca
     5221 ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg tttttaacca
     5281 gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac
     5341 cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc tcggccgcga
     5401 acggcctcac cccaaaaatg gcagcgctgg cagtccttgc cattgccggg atcggggcag
     5461 taacgggatg ggcgatcagc ccgagcgcga cgcccggaag cattgacgtg ccgcaggtgc
     5521 tggcatcgac attcagcgac caggtgccgg gcagtgaggg cggcggcctg ggtggcggcc
     5581 tgcccttcac ttcggccgtc ggggcattca cggacttcat ggcggggccg gcaattttta
     5641 ccttgggcat tcttggcata gtggtcgcgg gtgccgtgct cgtgttcggg ggtgcgataa
     5701 acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa acgagaattg
     5761 gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag acgaagagga
     5821 tgaagaggat gaggaggcag attgccttga atatattgac aatactgata agataatata
     5881 tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc ataggcagcg
     5941 cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg
     6001 aaaccaggac aataacctta tagcttgtaa attctatcat aattgggtaa tgactccaac
     6061 ttattgatag tgttttatgt tcagataatg cccgatgact ttgtcatgca gctccaccga
     6121 ttttgagaac gacagcgact tccgtcccag ccgtgccagg tgctgcctca gattcaggtt
     6181 atgccgctca attcgctgcg tatatcgctt gctgattacg tgcagctttc ccttcaggcg
     6241 ggattcatac agcggccagc catccgtcat ccatatcacc acgtcaaagg gtgacagcag
     6301 gctcataaga cgccccagcg tcgccatagt gcgttcaccg aatacgtgcg caacaaccgt
     6361 cttccggaga ctgtcatacg cgtaaaacag ccagcgctgg cgcgatttag ccccgacata
     6421 gccccactgt tcgtccattt ccgcgcagac gatgacgtca ctgcccggct gtatgcgcga
     6481 ggttaccgac tgcggcctga gttttttaag tgacgtaaaa tcgtgttgag gccaacgccc
     6541 ataatgcggg ctgttgcccg gcatccaacg ccattcatgg ccatatcaat gattttctgg
     6601 tgcgtaccgg gttgagaagc ggtgtaagtg aactgcagtt gccatgtttt acggcagtga
     6661 gagcagagat agcgctgatg tccggcggtg cttttgccgt tacgcaccac cccgtcagta
     6721 gctgaacagg agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat
     6781 catacactaa atcagtaagt tggcagcatc acccataatt gtggtttcaa aatcggctcc
     6841 gtcgatacta tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat
     6901 ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct
     6961 tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa
     7021 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
     7081 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
     7141 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
     7201 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
     7261 gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
     7321 aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg
     7381 acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact
     7441 tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta
     7501 aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct
     7561 tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct
     7621 ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc
     7681 ggtcgatcag ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg
     7741 ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagt
     7801 acctagatgt ggcgcaacga tgccggcgac aagcaggagc gcaccgactt cttccgcatc
     7861 aagtgttttg gctctcaggc cgaggcccac ggcaagtatt tgggcaaggg gtcgctggta
     7921 ttcgtgcagg gcaagattcg gaataccaag tacgagaagg acggccagac ggtctacggg
     7981 accgacttca ttgccgataa ggtggattat ctggacacca aggcaccagg cgggtcaaat
     8041 caggaataag ggcacattgc cccggcgtga gtcggggcaa tcccgcaagg agggtgaatg
     8101 aatcggacgt ttgaccggaa ggcatacagg caagaactga tcgacgcggg gttttccgcc
     8161 gaggatgccg aaaccatcgc aagccgcacc gtcatgcgtg cgccccgcga aaccttccag
     8221 tccgtcggct cgatggtcca gcaagctacg gccaagatcg agcgcgacag cgtgcaactg
     8281 gctccccctg ccctgcccgc gccatcggcc gccgtggagc gttcgcgtcg tctcgaacag
     8341 gaggcggcag gtttggcgaa gtcgatgacc atcgacacgc gaggaactat gacgaccaag
     8401 aagcgaaaaa ccgccggcga ggacctggca aaacaggtca gcgaggccaa gcaggccgcg
     8461 ttgctgaaac acacgaagca gcagatcaag gaaatgcagc tttccttgtt cgatattgcg
     8521 ccgtggccgg acacgatgcg agcgatgcca aacgacacgg cccgctctgc cctgttcacc
     8581 acgcgcaaca agaaaatccc gcgcgaggcg ctgcaaaaca aggtcatttt ccacgtcaac
     8641 aaggacgtga agatcaccta caccggcgtc gagctgcggg ccgacgatga cgaactggtg
     8701 tggcagcagg tgttggagta cgcgaagcgc acccctatcg gcgagccgat caccttcacg
     8761 ttctacgagc tttgccagga cctgggctgg tcgatcaatg gccggtatta cacgaaggcc
     8821 gaggaatgcc tgtcgcgcct acaggcgacg gcgatgggct tcacgtccga ccgcgttggg
     8881 cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc tggaccgtgg caagaaaacg
     8941 tcccgttgcc aggtcctgat cgacgaggaa atcgtcgtgc tgtttgctgg cgaccactac
     9001 acgaaattca tatgggagaa gtaccgcaag ctgtcgccga cggcccgacg gatgttcgac
     9061 tatttcagct cgcaccggga gccgtacccg ctcaagctgg aaaccttccg cctcatgtgc
     9121 ggatcggatt ccacccgcgt gaagaagtgg cgcgagcagg tcggcgaagc ctgcgaagag
     9181 ttgcgaggca gcggcctggt ggaacacgcc tgggtcaatg atgacctggt gcattgcaaa
     9241 cgctagggcc ttgtggggtc agttccggct gggggttcag cagccagcgc tttactggca
     9301 tttcaggaac aagcgggcac tgctcgacgc acttgcttcg ctcagtatcg ctcgggacgc
     9361 acggcgcgct ctacgaactg ccgataaaca gaggattaaa attgacaatt gtgattaagg
     9421 ctcagattcg acggcttgga gcggccgacg tgcaggattt ccgcgagatc cgattgtcgg
     9481 ccctgaagaa agctccagag atgttcgggt ccgtttacga gcacgaggag aaaaagccca
     9541 tggaggcgtt cgctgaacgg ttgcgagatg ccgtggcatt cggcgcctac atcgacggcg
     9601 agatcattgg gctgtcggtc ttcaaacagg aggacggccc caaggacgct cacaaggcgc
     9661 atctgtccgg cgttttcgtg gagcccgaac agcgaggccg aggggtcgcc ggtatgctgc
     9721 tgcgggcgtt gccggcgggt ttattgctcg tgatgatcgt ccgacagatt ccaacgggaa
     9781 tctggtggat gcgcatcttc atcctcggcg cacttaatat ttcgctattc tggagcttgt
     9841 tgtttatttc ggtctaccgc ctgccgggcg gggtcgcggc gacggtaggc gctgtgcagc
     9901 cgctgatggt cgtgttcatc tctgccgctc tgctaggtag cccgatacga ttgatggcgg
     9961 tcctgggggc tatttgcgga actgcgggcg tggcgctgtt ggtgttgaca ccaaacgcag
    10021 cgctagatcc tgtcggcgtc gcagcgggcc tggcgggggc ggtttccatg gcgttcggaa
    10081 ccgtgctgac ccgcaagtgg caacctcccg tgcctctgct cacctttacc gcctggcaac
    10141 tggcggccgg aggacttctg ctcgttccag tagctttagt gtttgatccg ccaatcccga
    10201 tgcctacagg aaccaatgtt ctcggcctgg cgtggctcgg cctgatcgga gcgggtttaa
    10261 cctacttcct ttggttccgg gggatctcgc gactcgaacc tacagttgtt tccttactgg
    10321 gctttctcag ccccagatct ggggtcgatc agccggggat gcatcaggcc gacagtcgga
    10381 acttcgggtc cccgacctgt accattcggt gagcaatgga taggggagtt gatatcgtca
    10441 acgttcactt ctaaagaaat agcgccactc agcttcctca gcggctttat ccagcgattt
    10501 cctattatgt cggcatagtt ctcaagatcg acagcctgtc acggttaagc gagaaatgaa
    10561 taagaaggct gataattcgg atctctgcga gggagatgat atttgatcac aggcagcaac
    10621 gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc
    10681 ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt
    10741 acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat
    10801 tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt
    10861 gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactgg
    10921 ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc
    10981 ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt
    11041 gatggtggtt ccgaaatcgg caaaatccct tataaatcaa aagaatagcc cgagataggg
    11101 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
    11161 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca
    11221 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
    11281 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
    11341 ggagcgggcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
    11401 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
    11461 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattaattc ccatcttgaa
    11521 agaaatatag tttaaatatt tattgataaa ataacaagtc aggtattata gtccaagcaa
    11581 aaacataaat ttattgatgc aagtttaaat tcagaaatat ttcaataact gattatatca
    11641 gctggtacat tgccgtagat gaaagactga gtgcgatatt atgtgtaata cataaattga
    11701 tgatatagct agcttagctc atcgggggat cgatccgtcg aagctagctt gggtcccgct
    11761 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
    11821 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
    11881 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
    11941 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
    12001 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
    12061 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
    12121 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
    12181 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
    12241 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
    12301 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
    12361 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
    12421 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
    12481 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
    12541 tcttgttcaa tccaagctcc catgggccct cgactagagt cgagatctgg attgagagtg
    12601 aatatgagac tctaattgga taccgagggg aatttatgga acgtcagtgg agcatttttg
    12661 acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg caataatggt
    12721 ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctgagt ggctccttca
    12781 acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt
    12841 cataacgtga ctcccttaat tctccgctca tgatcttgat cccctgcgcc atcagatcct
    12901 tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc
    12961 cccagctggc aattccggtt cgcttgctgt ccataaaacc gcccagtcta gctatcgcca
    13021 tgtaagccca ctgcaagcta cctgctttct ctttgcgctt gcgttttccc ttgtccagat
    13081 agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg ctttctacgt
    13141 gttccgcttc ctttagcagc ccttgcgccc tgagtgcttg cggcagcgtg aagcttcgac
    13201 gagtcagtaa taaacggcgt caaagtggtt gcagccggca cacacgagtc gtgtttatca
    13261 actcaaagca caaatacttt tcctcaacct aaaaataagg caattagcca aaaacaactt
    13321 tgcgtgtaaa caacgctcaa tacacgtgtc attttattat tagctattgc ttcaccgcct
    13381 tagctttctc gtgacctagt cgtcctcgtc ttttcttctt cttcttctat aaaacaatac
    13441 ccaaagagct cttcttcttc acaattcaga tttcaatttc tcaaaatctt aaaaactttc
    13501 tctcaattct ctctaccgtg atcaaggtaa atttctgtgt tccttattct ctcaaaatct
    13561 tcgattttgt tttcgttcga tcccaatttc gtatatgttc tttggtttag attctgttaa
    13621 tcttagatcg aagacgattt tctgggtttg atcgttagat atcatcttaa ttctcgatta
    13681 gggtttcata gatatcatcc gatttgttca aataatttga gttttgtcga ataattactc
    13741 ttcgatttgt gatttctatc tagttctggt gttagtttct agtttgtgcg atcgaatttg
    13801 tcgattaatc tgagtttttc tgattaacag actagcccgc ggaaaatgaa ggctcttact
    13861 gctagacaac aagaagtttt tgatcttatt agagatcata tttctcaaac tggaatgcct
    13921 cctactagag ctgaaattgc tcaaagactt ggatttaggt ctcctaatgc tgctgaagaa
    13981 catcttaagg ctcttgctag aaagggagtt attgaaattg tttctggagc ttcaagagga
    14041 attagacttc ttcaagaaga agaagaagga cttcctcttg ttggaagagt tgctgctgga
    14101 gaaccttctt ctgctcctcc tactgatgtt tctcttggag atgaacttca tcttgatgga
    14161 gaagatgttg ctatggctca tgctgatgct cttgatgatt ttgatcttga tatgcttgga
    14221 gatggagatt ctcctggacc tggatttact cctcatgatt ctgctcctta tggagcactt
    14281 gatatggctg attttgaatt tgaacaaatg tttactgatg ctcttggaat tgatgaatat
    14341 ggaggagatc cttctgctgg agatatgaga gctgctaatc tttggccttc tcctcttatg
    14401 attaagaggt ctaagaagaa ctctcttgct ctttctctta ctgctgacca aatggtttct
    14461 gctcttcttg atgctgaacc tcctattctt tattctgaat atgatcctac tagacctttt
    14521 tctgaggctt ctatgatggg acttcttact aatcttgctg atagagaact tgttcatatg
    14581 attaattggg ctaagagagt tcctggattt gttgatctta ctcttcatga ccaagttcat
    14641 cttcttgaat gtgcttggct tgaaattctt atgattggac ttgtttggag gtctatggaa
    14701 catcctgtta aacttctttt tgctcctaat cttcttcttg atagaaatca aggaaagtgt
    14761 gttgaaggaa tggttgaaat ttttgatatg cttcttgcta cttcttcaag atttagaatg
    14821 atgaatcttc aaggagaaga atttgtttgt cttaagtcta ttattcttct taattctgga
    14881 gtttatactt ttctttcttc tactcttaag tctcttgaag aaaaggatca tattcataga
    14941 gttcttgata agattactga tactcttatt catcttatgg ctaaggctgg acttactctt
    15001 caacaacaac atcaaagact tgctcaactt cttcttattc tttctcatat tagacatatg
    15061 tctaataagg gaatggaaca tctttattct atgaagtgta agaatgttgt tcctctttat
    15121 gatcttcttc ttgaaatgct tgatgctcat agacttcatg ctcctacttc aagaggagga
    15181 gcttctgttg aagaaactga ccaatctcat cttgctactg ctggatctac ttcttctcat
    15241 tctcttcaaa agtattatat tactggagaa gctgaaggat ttcctgctac tgtttgaaga
    15301 tcttgatcaa gctccctggc caattcccag agatgttagc tgaaatcatc actaatcaga
    15361 taccaaaata ttcaaatgga aatatcaaat agcttctgtt tcatcaaaaa tgactcgacc
    15421 taactgagta agcatgcttg ttcgagtatt atggcattgg gaaaactgtt tttcttgtac
    15481 catttgttgt gcttgtaatt tactgtgttt tttattcggt tttcgctatc gaactgtgaa
    15541 atggaaatgg atggagaaga gttaatgaat gatatggtcc ttttgttcat tctcaaatta
    15601 atattatttg ttttttctct tatttgttgt gtgttgaatt tgaaattata agagatatgc
    15661 aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc tctaatgacc gaagttaata
    15721 tgaggagtaa aacatcccaa acgcggccgc aaccggtctt gcatccagct gggctgcagg
    15781 tcgaggctaa aaaactaatc gcattatcat cccctcgacg tactgtacat ataaccactg
    15841 gttttatata cagcagtact gtacatataa ccactggttt tatatacagc agtcgacgta
    15901 ctgtacatat aaccactggt tttatataca gcagtactgt acatataacc actggtttta
    15961 tatacagcag tcgaggtaag attagatatg gatatgtata tggatatgta tatggtggta
    16021 atgc
//
