LOCUS       sequence-208559-        8727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of ABE7.10 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Liu Z, Lu Z, Yang G, Huang S, Li G, Feng S, Liu Y, Li J, Yu W, Zhang
            Y, Chen J, Sun Q, Huang X
  TITLE     Efficient generation of mouse models of human diseases via ABE- and 
            BE-mediated base editing.
  JOURNAL   Nat Commun. 2018 Jun 14;9(1):2338. doi: 10.1038/s41467-018-04768-7.
  PUBMED    29904106
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-14"; volume: "9(1):2338. doi"; pages: " 
            10.1038/s41467-018-04768-7"
COMMENT     Sequence Label: pCMV-ABE
FEATURES             Location/Qualifiers
     source          1..8727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             658..1158
                     /codon_start=1
                     /product="tRNA adenosine deaminase from E. coli"
                     /label=TadA
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEGW
                     NRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRVVF
                     GARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQKKAQ
                     SSTD"
     CDS             1255..1752
                     /codon_start=1
                     /product="DNA adenosine deaminase evolved from the E. coli 
                     tRNA adenosine deaminase TadA (Gaudelli et al., 2017)"
                     /label=TadA*(7.10)
                     /translation="SEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWN
                     RAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFG
                     VRNAKTGAAGSLMDVLHYPGMNHRVEITEGILADECAALLCYFFRMPRQVFNAQKKAQS
                     STD"
     CDS             join(1849..5247,5248..5250,5251..5847,5848,5849..5851,
                     5852..5853,5854..5856,5857..5949)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1849..1872,1873..1875,1876..5949)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5962..5982
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5991..6008
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6031..6048)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6037..6261
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6332..6348)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6332..6348)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6345..6367)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6356..6372
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6380..6386,6387..6404,6405..6410))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6425..6446
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6563..6580)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6734..7322)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6814..6833)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7493..8284,8285..8353))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8116..8135
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8354..8458)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8537..8556)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 tccgaagtcg agttttccca tgagtactgg atgagacacg cattgactct cgcaaagagg
      721 gcttgggatg aacgcgaggt gcccgtgggg gcagtactcg tgcataacaa tcgcgtaatc
      781 ggcgaaggtt ggaataggcc gatcggacgc cacgacccca ctgcacatgc ggaaatcatg
      841 gcccttcgac agggagggct tgtgatgcag aattatcgac ttatcgatgc gacgctgtac
      901 gtcacgcttg aaccttgcgt aatgtgcgcg ggagctatga ttcactcccg cattggacga
      961 gttgtattcg gtgcccgcga cgccaagacg ggtgccgcag gttcactgat ggacgtgctg
     1021 catcacccag gcatgaacca ccgggtagaa atcacagaag gcatattggc ggacgaatgt
     1081 gcggcgctgt tgtccgactt ttttcgcatg cggaggcagg agatcaaggc ccagaaaaaa
     1141 gcacaatcct ctactgacag cggcggcagc agcggcggca gcagcggcag cgagactccc
     1201 gggacctcag agtccgccac acccgaaagt agcggcggca gcagcggcgg cagctccgaa
     1261 gtcgagtttt cccatgagta ctggatgaga cacgcattga ctctcgcaaa gagggctcgg
     1321 gatgaacgcg aggtgcccgt gggggcagta ctcgtgctta acaatcgcgt aatcggcgaa
     1381 ggttggaata gggcgatcgg actccacgac cccactgcac atgcggaaat catggccctt
     1441 cgacagggag ggcttgtgat gcagaattat cgacttatcg atgcgacgct gtacgtcacg
     1501 tttgaacctt gcgtaatgtg cgcgggagct atgattcact cccgcattgg acgagttgta
     1561 ttcggtgtcc gcaacgccaa gacgggtgcc gcaggttcac tgatggacgt gctgcattac
     1621 ccaggcatga accaccgggt agaaatcaca gaaggcatat tggcggacga atgtgcggcg
     1681 ctgttgtgct acttttttcg catgccgagg caggtgttca atgcccagaa aaaagcacaa
     1741 tcctctactg acagcggcgg cagcagcggc ggcagcagcg gcagcgagac tcccgggacc
     1801 tcagagtccg ccacacccga aagtagcggc ggcagcagcg gcggcagcga taaaaagtat
     1861 tctattggtt tagccatcgg cactaattcc gttggatggg ctgtcataac cgatgaatac
     1921 aaagtacctt caaagaaatt taaggtgttg gggaacacag accgtcattc gattaaaaag
     1981 aatcttatcg gtgccctcct attcgatagt ggcgaaacgg cagaggcgac tcgcctgaaa
     2041 cgaaccgctc ggagaaggta tacacgtcgc aagaaccgaa tatgttactt acaagaaatt
     2101 tttagcaatg agatggccaa agttgacgat tctttctttc accgtttgga agagtccttc
     2161 cttgtcgaag aggacaagaa acatgaacgg caccccatct ttggaaacat agtagatgag
     2221 gtggcatatc atgaaaagta cccaacgatt tatcacctca gaaaaaagct agttgactca
     2281 actgataaag cggacctgag gttaatctac ttggctcttg cccatatgat aaagttccgt
     2341 gggcactttc tcattgaggg tgatctaaat ccggacaact cggatgtcga caaactgttc
     2401 atccagttag tacaaaccta taatcagttg tttgaagaga accctataaa tgcaagtggc
     2461 gtggatgcga aggctattct tagcgcccgc ctctctaaat cccgacggct agaaaacctg
     2521 atcgcacaat tacccggaga gaagaaaaat gggttgttcg gtaaccttat agcgctctca
     2581 ctaggcctga caccaaattt taagtcgaac ttcgacttag ctgaagatgc caaattgcag
     2641 cttagtaagg acacgtacga tgacgatctc gacaatctac tggcacaaat tggagatcag
     2701 tatgcggact tatttttggc tgccaaaaac cttagcgatg caatcctcct atctgacata
     2761 ctgagagtta atactgagat taccaaggcg ccgttatccg cttcaatgat caaaaggtac
     2821 gatgaacatc accaagactt gacacttctc aaggccctag tccgtcagca actgcctgag
     2881 aaatataagg aaatattctt tgatcagtcg aaaaacgggt acgcaggtta tattgacggc
     2941 ggagcgagtc aagaggaatt ctacaagttt atcaaaccca tattagagaa gatggatggg
     3001 acggaagagt tgcttgtaaa actcaatcgc gaagatctac tgcgaaagca gcggactttc
     3061 gacaacggta gcattccaca tcaaatccac ttaggcgaat tgcatgctat acttagaagg
     3121 caggaggatt tttatccgtt cctcaaagac aatcgtgaaa agattgagaa aatcctaacc
     3181 tttcgcatac cttactatgt gggacccctg gcccgaggga actctcggtt cgcatggatg
     3241 acaagaaagt ccgaagaaac gattactcca tggaattttg aggaagttgt cgataaaggt
     3301 gcgtcagctc aatcgttcat cgagaggatg accaactttg acaagaattt accgaacgaa
     3361 aaagtattgc ctaagcacag tttactttac gagtatttca cagtgtacaa tgaactcacg
     3421 aaagttaagt atgtcactga gggcatgcgt aaacccgcct ttctaagcgg agaacagaag
     3481 aaagcaatag tagatctgtt attcaagacc aaccgcaaag tgacagttaa gcaattgaaa
     3541 gaggactact ttaagaaaat tgaatgcttc gattctgtcg agatctccgg ggtagaagat
     3601 cgatttaatg cgtcacttgg tacgtatcat gacctcctaa agataattaa agataaggac
     3661 ttcctggata acgaagagaa tgaagatatc ttagaagata tagtgttgac tcttaccctc
     3721 tttgaagatc gggaaatgat tgaggaaaga ctaaaaacat acgctcacct gttcgacgat
     3781 aaggttatga aacagttaaa gaggcgtcgc tatacgggct ggggacgatt gtcgcggaaa
     3841 cttatcaacg ggataagaga caagcaaagt ggtaaaacta ttctcgattt tctaaagagc
     3901 gacggcttcg ccaataggaa ctttatgcag ctgatccatg atgactcttt aaccttcaaa
     3961 gaggatatac aaaaggcaca ggtttccgga caaggggact cattgcacga acatattgcg
     4021 aatcttgctg gttcgccagc catcaaaaag ggcatactcc agacagtcaa agtagtggat
     4081 gagctagtta aggtcatggg acgtcacaaa ccggaaaaca ttgtaatcga gatggcacgc
     4141 gaaaatcaaa cgactcagaa ggggcaaaaa aacagtcgag agcggatgaa gagaatagaa
     4201 gagggtatta aagaactggg cagccagatc ttaaaggagc atcctgtgga aaatacccaa
     4261 ttgcagaacg agaaacttta cctctattac ctacaaaatg gaagggacat gtatgttgat
     4321 caggaactgg acataaaccg tttatctgat tacgacgtcg atcacattgt accccaatcc
     4381 tttttgaagg acgattcaat cgacaataaa gtgcttacac gctcggataa gaaccgaggg
     4441 aaaagtgaca atgttccaag cgaggaagtc gtaaagaaaa tgaagaacta ttggcggcag
     4501 ctcctaaatg cgaaactgat aacgcaaaga aagttcgata acttaactaa agctgagagg
     4561 ggtggcttgt ctgaacttga caaggccgga tttattaaac gtcagctcgt ggaaacccgc
     4621 caaatcacaa agcatgttgc acagatacta gattcccgaa tgaatacgaa atacgacgag
     4681 aacgataagc tgattcggga agtcaaagta atcactttaa agtcaaaatt ggtgtcggac
     4741 ttcagaaagg attttcaatt ctataaagtt agggagataa ataactacca ccatgcgcac
     4801 gacgcttatc ttaatgccgt cgtagggacc gcactcatta agaaataccc gaagctagaa
     4861 agtgagtttg tgtatggtga ttacaaagtt tatgacgtcc gtaagatgat cgcgaaaagc
     4921 gaacaggaga taggcaaggc tacagccaaa tacttctttt attctaacat tatgaatttc
     4981 tttaagacgg aaatcactct ggcaaacgga gagatacgca aacgaccttt aattgaaacc
     5041 aatggggaga caggtgaaat cgtatgggat aagggccggg acttcgcgac ggtgagaaaa
     5101 gttttgtcca tgccccaagt caacatagta aagaaaactg aggtgcagac cggagggttt
     5161 tcaaaggaat cgattcttcc aaaaaggaat agtgataagc tcatcgctcg taaaaaggac
     5221 tgggacccga aaaagtacgg tggcttcgat agccctacag ttgcctattc tgtcctagta
     5281 gtggcaaaag ttgagaaggg aaaatccaag aaactgaagt cagtcaaaga attattgggg
     5341 ataacgatta tggagcgctc gtcttttgaa aagaacccca tcgacttcct tgaggcgaaa
     5401 ggttacaagg aagtaaaaaa ggatctcata attaaactac caaagtatag tctgtttgag
     5461 ttagaaaatg gccgaaaacg gatgttggct agcgccggag agcttcaaaa ggggaacgaa
     5521 ctcgcactac cgtctaaata cgtgaatttc ctgtatttag cgtcccatta cgagaagttg
     5581 aaaggttcac ctgaagataa cgaacagaag caactttttg ttgagcagca caaacattat
     5641 ctcgacgaaa tcatagagca aatttcggaa ttcagtaaga gagtcatcct agctgatgcc
     5701 aatctggaca aagtattaag cgcatacaac aagcacaggg ataaacccat acgtgagcag
     5761 gcggaaaata ttatccattt gtttactctt accaacctcg gcgctccagc cgcattcaag
     5821 tattttgaca caacgataga tcgcaaacga tacacttcta ccaaggaggt gctagacgcg
     5881 acactgattc accaatccat cacgggatta tatgaaactc ggatagattt gtcacagctt
     5941 gggggtgact ctggtggttc tcccaagaag aagaggaaag tctaaccggt catcatcacc
     6001 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     6061 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     6121 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     6181 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     6241 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tcgataccgt
     6301 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6361 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctagggtg
     6421 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6481 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6541 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6601 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6661 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6721 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6781 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6841 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6901 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6961 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7021 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7081 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7141 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7201 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7261 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7321 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7381 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7441 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7501 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7561 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7621 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7681 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7741 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7801 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7861 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7921 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7981 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8041 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8101 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8161 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8221 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8281 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8341 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8401 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8461 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcgatct cccgatcccc
     8521 tagggtcgac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     8581 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     8641 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     8701 gcgatgtacg ggccagatat acgcgtt
//
