LOCUS       sequence-208592-        6349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for expressing proteins in a subset of 9 DA/ DB cholingergic 
            motor neurons in the C. elegans ventral nerve cord.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Edwards SL, Charlie NK, Richmond JE, Hegermann J, Eimer S, Miller KG
  TITLE     Impaired dense core vesicle maturation in Caenorhabditis elegans 
            mutants lacking Rab2.
  JOURNAL   J Cell Biol. 2009 Sep 21;186(6):881-95. doi: 10.1083/jcb.200902095.
  PUBMED    19797080
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2009-09-21- 21"; volume: "186"; 
            issue: "6"; pages: "881-95"
COMMENT     Sequence Label: KG#230
FEATURES             Location/Qualifiers
     source          1..6349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3789..3811)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3911..3930
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4008..4026)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4094..4198
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4199..4267,4268..5059)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4417..4436)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5230..5818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5719..5738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5972..5989
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6106..6127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6142..6147,6148..6165,6166..6172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6180..6196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6185..6207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6204..6220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6204..6220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagcccgg gaaacatgat atcgacggac atatttttga ttgattatca
       61 taattttcgt gggaatcttc ggaacatgct ctttgcggtt tataagtgga gatgcgccaa
      121 cgggaaactt gttttaaaac tattcctatg gtgctaacat caccacatga aaacttttca
      181 aaactttttt gggtttttta tttttttatt attattcgta tctaaacatt gtaggcgttg
      241 tgatgttgcc ctaaatttct ttaaaaagag cgaacatttt caaaaatttg ctatttaaat
      301 cgaatgaaaa tttccattca aatatttgaa aaaaattatg aaaaaattcc ttttgttgca
      361 tcttcatctt ctatgaaagt tacaatttta gttggatcag aaatatgaga tatcaaaaaa
      421 aaattaagat ttttttgttt gtttttttga agcctaaaat attttgatat catttatctc
      481 ttatgtcttt tgttttatcg aaacgtttac aacacatcaa ctatgcgtca ataaattttt
      541 taggtagtta aacaaatgtt gaaaaaaaaa gcaaaagcaa ccaagagctg ttaaaataga
      601 gaaaatgcag ttatttttta tattcaagaa aagttatcac agaagttccg taatgagttt
      661 tttccttatc aacatgttcc catcaacttc ttagtttctc ctcattccca ttgttcaaca
      721 taattatcac ctctgacctt attctgctct ttttaagcac aggtgttaat atgccgtcca
      781 tcatatttgc cctccgtctg ccgctttcct tatctgacct ttacatcgcc tttttgtata
      841 tgtacataat aattgtatca tcatcaacct ccgccataaa catgcatgtc tttttacctc
      901 ttttggcatg taccgttctt ctttgtattt ttattatgtc ttccttccat cttccctctt
      961 ttttacggat tttcggattg cggaagaaga agatggtcga taattaccgt aatgaggtaa
     1021 tttgaaacat attggacaga agaaatacgc gcggatgctt tcggtggcaa ggttaattcg
     1081 ttgtctttgt ttctggaatg tccttcataa aaaacacgga ttaccacctg tttttaacgt
     1141 ggattaagat ttttggaaac aatttactct gcctaatttt tttgtacatt taaaaatgta
     1201 gacttaccga attcaccgag cttgaccagt tttaaaggtt accgttcaca ctaaaaattg
     1261 ttgaaaaaat tttcgactaa acctaatgat tttattttaa aaaataaatg aaatagcata
     1321 caaatttgga tttttttcaa tttttttcaa gaaaatcatg aaacatttag aaaactcgag
     1381 taacaagctt tccgttcatt ctgtaatttt taagttccaa atttaaaaaa atgcattttg
     1441 atttactggg attgccgtgc tcgtaattat cagcaaaaca tttgccgaat tagccgagct
     1501 cgacaatcta ttttttcgca aacttttgcc atgtttcgta aaacaatttt ccattttatg
     1561 gtagacattt ttcatcaatg aattcacaaa atttaaaatc cccactgaaa gtaccaaaat
     1621 cttcctgaaa aatccggcaa aaaatccatc tcattatcat gtcacatata catgttctat
     1681 tggcactatg tttgggatgt cctccaatta atacttctgt gtcaatctca tcggcgcccg
     1741 gcggccacta ccaaatgcaa aactttgttt gtaaaagaag aagccaataa ttttctcgtt
     1801 attcattttt attactcttt ttgcgtttca ttcttctttg ttcgaattga tgaacgcacg
     1861 ttgcttctct ctggttttcc tgccgttcct tatattgcca aggaaaccaa tgcggatttt
     1921 cggaagattt tcttcatgtc ctttagaaaa tatcaccact tttcttcttt ttccatttca
     1981 aatttcaatt tccatacaac tcatcttcta ttatagcttt tcaggtgttc gactgtttga
     2041 agataagaat caattaaaat ttggttcact gagcatgtca tccacttttc cttttctttg
     2101 ttctaaaatt caccaaactg aattttcctt aaccggaaat ttacatgaaa ataagaaaca
     2161 tggattacat cctttacata tcatttttgt ttttttatgt gtattccatg ttcccaaaag
     2221 tttgatgcaa tttttgattc tttttcaatt ttccaaattc gtgtctcgca gggaacgatt
     2281 cacagtccaa cattcccaaa ttgcgaaatt ttagaatata tattcccaag cgtcgaatat
     2341 ctcaaaaatt tgtcgttttc tcagtttgac atgtcacctt tgccggttcc tctattcgtc
     2401 tattagctct ccagagagca atgtcatgtc acacgaagaa ttcagggaca tgtttcgttt
     2461 ttttttgttt gcatgtggtc accgatatgg gaacttgaac tttccaattt ttgcttgctt
     2521 tttcttttcc ggaaacttaa tactatttga gaaactccgc atcttatttg tttgatccca
     2581 aattctgaaa ttcctctttg aatttgatct caagcttcac tataaaaaat tgtttcagga
     2641 aaattggaag agcaagcgga tccccgggat tggccaaagg acccaaaggt atgtttcgaa
     2701 tgatactaac ataacataga acattttcag gaggaccctt ggctagcgtc gacggtaccg
     2761 gtcagatatc actcgaggag ctcccgagat cttcagcgct cagcattcgt agaattgtaa
     2821 gtttaaactt tctcatacta actaacgcgc tctatttaaa ttttcagaat tccaactgag
     2881 cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa taataggggc cgctgtcatc
     2941 agagtaagtt taaactgagt tctactaact aacgagtaat atttaaattt tcagcatctc
     3001 gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca tgctctttct
     3061 ccctgtgctc ccacccccta tttttgttat tatcaaaaaa acttcttctt aatttctttg
     3121 ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca aaaatagaat
     3181 taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat aataattcta
     3241 tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttttact tctgataaat
     3301 tttttttgaa acatcataga aaaaaccgca cacaaaatac cttatcatat gttacgtttc
     3361 agtttatgac cgcaattttt atttcttcgc acgtctgggc ctctcatgac gtcaaatcat
     3421 gctcatcgtg aaaaagtttt ggagtatttt tggaattttt caatcaagtg aaagtttatg
     3481 aaattaattt tcctgctttt gctttttggg ggtttcccct attgtttgtc aagagtttcg
     3541 aggacggcgt ttttcttgct aaaatcacaa gtattgatga gcacgatgca agaaagatcg
     3601 gaagaaggtt tgggtttgag gctcagtgga aggtgagtag aagttgataa tttgaaagtg
     3661 gagtagtgtc tatggggttt ttgccttaaa tgacagaata cattcccaat ataccaaaca
     3721 taactgtttc ctactagtcg gccgtacggg ccctttcgtc tcgcgcgttt cggtgatgac
     3781 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     3841 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     3901 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     3961 ccgcacagat gcgtaaggag aaaataccgc atcaggcggc cttaagggcc tcgtgatacg
     4021 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     4081 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4141 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4201 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4261 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4321 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4381 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4441 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     4501 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     4561 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     4621 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     4681 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     4741 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     4801 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     4861 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     4921 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     4981 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5041 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5101 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5161 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5221 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5281 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5341 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     5401 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5461 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5521 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5581 gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct
     5641 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5701 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5761 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5821 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     5881 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     5941 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     6001 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     6061 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     6121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     6181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctgta
     6241 agtttaaaca tgatcttact aactaactat tctcatttaa attttcagag cttaaaaatg
     6301 gctgaaatca ctcacaacga tggatacgct aacaacttgg aaatgaaat
//
