LOCUS       sequence-208593-        4123 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for expressing proteins in most or all tissues, including 
            nervous system, under control of the inducible heat shock promoter 
            in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Reynolds NK, Schade MA, Miller KG
  TITLE     Convergent, RIC-8-dependent Galpha signaling pathways in the 
            Caenorhabditis elegans synaptic signaling network.
  JOURNAL   Genetics. 2005 Feb;169(2):651-70. doi: 10.1534/genetics.104.031286. 
            Epub 2004 Oct 16.
  PUBMED    15489511
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.104.031286"; journalName: "Genetics"; date: 
            "2005-02"; volume: "169"; issue: "2"; pages: "651-70"
COMMENT     Sequence Label: KG#45
FEATURES             Location/Qualifiers
     source          1..4123
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1563..1585)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1685..1704
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1782..1800)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1868..1972
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1973..2041,2042..2833)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2191..2210)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3004..3592
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3493..3512
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3746..3763
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3880..3901
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3916..3921,3922..3939,3940..3946)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3954..3970
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3959..3981
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3978..3994
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3978..3994
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatcaagag catttgaatc agaatatgga
       61 gaacggagca tgagcatttt cgaagttttt tagatgcact agaacaaagc gtgttggctt
      121 cctctgagcc cgctttcctt atatacccgc attctgcagc cttacagaat gttctagaag
      181 gtcctagatg cattcgtttg aaaatactcc cggtgggtgc aaagagacgc agacggaaaa
      241 tgtatctggg tctctttatt gtgtacacta cttttccatg taccgaatgt gagtcgccct
      301 ccttttgcaa caagcagctc gaatgttcta gaaaaaggtg gaaaatagta taaataccgt
      361 tgaaaataaa taccgaacaa catttgctct aattgtgaaa ttagaaatct tcaaactata
      421 atcatctcac tggatccccg ggattggcca aaggacccaa aggtatgttt cgaatgatac
      481 taacataaca tagaacattt tcaggaggac ccttggctag cgtcgacggt accggtcaga
      541 tatcactcga ggagctcccg agatcttcag cgctcagcat tcgtagaatt gtaagtttaa
      601 actttctcat actaactaac gcgctctatt taaattttca gaattccaac tgagcgccgg
      661 tcgctaccat taccaacttg tctggtgtca aaaataatag gggccgctgt catcagagta
      721 agtttaaact gagttctact aactaacgag taatatttaa attttcagca tctcgcgccc
      781 gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct ttctccctgt
      841 gctcccaccc cctatttttg ttattatcaa aaaaacttct tcttaatttc tttgtttttt
      901 agcttctttt aagtcacctc taacaatgaa attgtgtaga ttcaaaaata gaattaattc
      961 gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat taataataat tctatcccaa
     1021 aatctacaca atgttctgtg tacacttctt atgttttttt tacttctgat aaattttttt
     1081 tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg tttcagttta
     1141 tgaccgcaat ttttatttct tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat
     1201 cgtgaaaaag ttttggagta tttttggaat ttttcaatca agtgaaagtt tatgaaatta
     1261 attttcctgc ttttgctttt tgggggtttc ccctattgtt tgtcaagagt ttcgaggacg
     1321 gcgtttttct tgctaaaatc acaagtattg atgagcacga tgcaagaaag atcggaagaa
     1381 ggtttgggtt tgaggctcag tggaaggtga gtagaagttg ataatttgaa agtggagtag
     1441 tgtctatggg gtttttgcct taaatgacag aatacattcc caatatacca aacataactg
     1501 tttcctacta gtcggccgta cgggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     1561 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     1621 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     1681 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     1741 agatgcgtaa ggagaaaata ccgcatcagg cggccttaag ggcctcgtga tacgcctatt
     1801 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     1861 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1921 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     1981 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2041 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     2101 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2161 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     2221 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2281 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2341 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     2401 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2461 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2521 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     2581 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2641 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2701 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2761 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2821 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2881 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2941 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3001 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3061 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3121 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     3181 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3241 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3301 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3361 gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga
     3421 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3481 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3541 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3601 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3661 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3721 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     3781 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     3841 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     3901 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     3961 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tgtaagttta
     4021 aacatgatct tactaactaa ctattctcat ttaaattttc agagcttaaa aatggctgaa
     4081 atcactcaca acgatggata cgctaacaac ttggaaatga aat
//
