LOCUS       sequence-208594-        6360 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for expressing proteins in a subset of 9 DA/ DB cholingergic 
            motor neurons in the C. elegans ventral nerve cord.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Edwards SL, Charlie NK, Richmond JE, Hegermann J, Eimer S, Miller KG
  TITLE     Impaired dense core vesicle maturation in Caenorhabditis elegans 
            mutants lacking Rab2.
  JOURNAL   J Cell Biol. 2009 Sep 21;186(6):881-95. doi: 10.1083/jcb.200902095.
  PUBMED    19797080
REFERENCE   2  (bases 1 to 6360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2009-09-21- 21"; volume: "186"; 
            issue: "6"; pages: "881-95"
COMMENT     Sequence Label: KG#244
FEATURES             Location/Qualifiers
     source          1..6360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3800..3822)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3922..3941
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4019..4037)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4105..4209
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4210..4278,4279..5070)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4428..4447)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5241..5829
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5730..5749
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5983..6000
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6117..6138
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6153..6158,6159..6176,6177..6183)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6191..6207
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6196..6218
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6215..6231
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6215..6231
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagcccgg gaaacatgat atcgacggac atatttttga ttgattatca
       61 taattttcgt gggaatcttc ggaacatgct ctttgcggtt tataagtgga gatgcgccaa
      121 cgggaaactt gttttaaaac tattcctatg gtgctaacat caccacatga aaacttttca
      181 aaactttttt gggtttttta tttttttatt attattcgta tctaaacatt gtaggcgttg
      241 tgatgttgcc ctaaatttct ttaaaaagag cgaacatttt caaaaatttg ctatttaaat
      301 cgaatgaaaa tttccattca aatatttgaa aaaaattatg aaaaaattcc ttttgttgca
      361 tcttcatctt ctatgaaagt tacaatttta gttggatcag aaatatgaga tatcaaaaaa
      421 aaattaagat ttttttgttt gtttttttga agcctaaaat attttgatat catttatctc
      481 ttatgtcttt tgttttatcg aaacgtttac aacacatcaa ctatgcgtca ataaattttt
      541 taggtagtta aacaaatgtt gaaaaaaaaa gcaaaagcaa ccaagagctg ttaaaataga
      601 gaaaatgcag ttatttttta tattcaagaa aagttatcac agaagttccg taatgagttt
      661 tttccttatc aacatgttcc catcaacttc ttagtttctc ctcattccca ttgttcaaca
      721 taattatcac ctctgacctt attctgctct ttttaagcac aggtgttaat atgccgtcca
      781 tcatatttgc cctccgtctg ccgctttcct tatctgacct ttacatcgcc tttttgtata
      841 tgtacataat aattgtatca tcatcaacct ccgccataaa catgcatgtc tttttacctc
      901 ttttggcatg taccgttctt ctttgtattt ttattatgtc ttccttccat cttccctctt
      961 ttttacggat tttcggattg cggaagaaga agatggtcga taattaccgt aatgaggtaa
     1021 tttgaaacat attggacaga agaaatacgc gcggatgctt tcggtggcaa ggttaattcg
     1081 ttgtctttgt ttctggaatg tccttcataa aaaacacgga ttaccacctg tttttaacgt
     1141 ggattaagat ttttggaaac aatttactct gcctaatttt tttgtacatt taaaaatgta
     1201 gacttaccga attcaccgag cttgaccagt tttaaaggtt accgttcaca ctaaaaattg
     1261 ttgaaaaaat tttcgactaa acctaatgat tttattttaa aaaataaatg aaatagcata
     1321 caaatttgga tttttttcaa tttttttcaa gaaaatcatg aaacatttag aaaactcgag
     1381 taacaagctt tccgttcatt ctgtaatttt taagttccaa atttaaaaaa atgcattttg
     1441 atttactggg attgccgtgc tcgtaattat cagcaaaaca tttgccgaat tagccgagct
     1501 cgacaatcta ttttttcgca aacttttgcc atgtttcgta aaacaatttt ccattttatg
     1561 gtagacattt ttcatcaatg aattcacaaa atttaaaatc cccactgaaa gtaccaaaat
     1621 cttcctgaaa aatccggcaa aaaatccatc tcattatcat gtcacatata catgttctat
     1681 tggcactatg tttgggatgt cctccaatta atacttctgt gtcaatctca tcggcgcccg
     1741 gcggccacta ccaaatgcaa aactttgttt gtaaaagaag aagccaataa ttttctcgtt
     1801 attcattttt attactcttt ttgcgtttca ttcttctttg ttcgaattga tgaacgcacg
     1861 ttgcttctct ctggttttcc tgccgttcct tatattgcca aggaaaccaa tgcggatttt
     1921 cggaagattt tcttcatgtc ctttagaaaa tatcaccact tttcttcttt ttccatttca
     1981 aatttcaatt tccatacaac tcatcttcta ttatagcttt tcaggtgttc gactgtttga
     2041 agataagaat caattaaaat ttggttcact gagcatgtca tccacttttc cttttctttg
     2101 ttctaaaatt caccaaactg aattttcctt aaccggaaat ttacatgaaa ataagaaaca
     2161 tggattacat cctttacata tcatttttgt ttttttatgt gtattccatg ttcccaaaag
     2221 tttgatgcaa tttttgattc tttttcaatt ttccaaattc gtgtctcgca gggaacgatt
     2281 cacagtccaa cattcccaaa ttgcgaaatt ttagaatata tattcccaag cgtcgaatat
     2341 ctcaaaaatt tgtcgttttc tcagtttgac atgtcacctt tgccggttcc tctattcgtc
     2401 tattagctct ccagagagca atgtcatgtc acacgaagaa ttcagggaca tgtttcgttt
     2461 ttttttgttt gcatgtggtc accgatatgg gaacttgaac tttccaattt ttgcttgctt
     2521 tttcttttcc ggaaacttaa tactatttga gaaactccgc atcttatttg tttgatccca
     2581 aattctgaaa ttcctctttg aatttgatct caagcttcac tataaaaaat tgtttcagga
     2641 aaattggaag agcaagcgga tccccgggat tggccaaagg acccaaaggt atgtttcgaa
     2701 tgatactaac ataacataga acattttcag gaggaccctt ggctagcgtc gacggtaccg
     2761 gtcagatatc actcgagact tggcggccgc aatcggaaga tcttcagcgc tcagcattcg
     2821 tagaattgta agtttaaact ttctcatact aactaacgcg ctctatttaa attttcagaa
     2881 ttccaactga gcgccggtcg ctaccattac caacttgtct ggtgtcaaaa ataatagggg
     2941 ccgctgtcat cagagtaagt ttaaactgag ttctactaac taacgagtaa tatttaaatt
     3001 ttcagcatct cgcgcccgtg cctctgactt ctaagtccaa ttactcttca acatccctac
     3061 atgctctttc tccctgtgct cccaccccct atttttgtta ttatcaaaaa aacttcttct
     3121 taatttcttt gttttttagc ttcttttaag tcacctctaa caatgaaatt gtgtagattc
     3181 aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa
     3241 taataattct atcccaaaat ctacacaatg ttctgtgtac acttcttatg ttttttttac
     3301 ttctgataaa ttttttttga aacatcatag aaaaaaccgc acacaaaata ccttatcata
     3361 tgttacgttt cagtttatga ccgcaatttt tatttcttcg cacgtctggg cctctcatga
     3421 cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt ttggaatttt tcaatcaagt
     3481 gaaagtttat gaaattaatt ttcctgcttt tgctttttgg gggtttcccc tattgtttgt
     3541 caagagtttc gaggacggcg tttttcttgc taaaatcaca agtattgatg agcacgatgc
     3601 aagaaagatc ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta gaagttgata
     3661 atttgaaagt ggagtagtgt ctatggggtt tttgccttaa atgacagaat acattcccaa
     3721 tataccaaac ataactgttt cctactagtc ggccgtacgg gccctttcgt ctcgcgcgtt
     3781 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     3841 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     3901 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     3961 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgg ccttaagggc
     4021 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     4081 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4141 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4201 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4261 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4321 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4381 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4441 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4501 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     4561 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     4621 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     4681 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     4741 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     4801 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4861 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4921 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     4981 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5041 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5101 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5161 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5221 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5281 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5341 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     5401 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5461 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5521 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5581 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa
     5641 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5701 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5761 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5821 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5881 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     5941 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6001 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     6061 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     6121 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     6181 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     6241 gccaagctgt aagtttaaac atgatcttac taactaacta ttctcattta aattttcaga
     6301 gcttaaaaat ggctgaaatc actcacaacg atggatacgc taacaacttg gaaatgaaat
//
