LOCUS       sequence-208600-        4915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses proteins pan-neuronally in C. elegans (entire nervous 
            system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Schade MA, Reynolds NK, Dollins CM, Miller KG
  TITLE     Mutations that rescue the paralysis of Caenorhabditis elegans ric-8 
            (synembryn) mutants activate the G alpha(s) pathway and define a 
            third major branch of the synaptic signaling network.
  JOURNAL   Genetics. 2005 Feb;169(2):631-49. doi: 10.1534/genetics.104.032334. 
            Epub 2004 Oct 16.
  PUBMED    15489510
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.104.032334"; journalName: "Genetics"; date: 
            "2005-02"; volume: "169"; issue: "2"; pages: "631-49"
COMMENT     Sequence Label: KG#59
FEATURES             Location/Qualifiers
     source          1..4915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2355..2377)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2477..2496
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2574..2592)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2660..2764
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2765..2833,2834..3625)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2983..3002)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3796..4384
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4285..4304
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4538..4555
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4672..4693
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4708..4713,4714..4731,4732..4738)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4746..4762
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4751..4773
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4770..4786
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4770..4786
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcaggatct tcagatggga gcagtggact gtctatttga tattcttaaa
       61 atgtactcta taaaagatga aactgtaatg accaattccc tacataaata tttttaacta
      121 attggtcgga tatttgggga tcagagaagt acttgttttt gcgatattaa taatgatata
      181 tttttctagt aaaaaaatgt taagcgattt gtgaataatt aagttggctt gaaaaagatt
      241 ttttgatagc tatgtatttt gttaatttaa atcagatttt aaaactttat tattgactat
      301 ttatatatat tatatattat atattttcat ggtttaatca aaatctatta aattatttct
      361 aaaatctcta aatatcgaat tctctaaaaa gcttcatcaa caaaagcatt tcaattatct
      421 aacacaatga gaataattca agaactgaaa tagaaagaaa tctagaaaaa aaagagagtg
      481 aggtgcccta ttaaatcggt gctctcctct ctaacagaag cgcctacacg cacccaagag
      541 agctcttttt ctttcttttt tcggagcaca catacacgca tacactcctc agaagtgtat
      601 agagaaaatg ggagaagaag cgaaagaaga cgaagaagcg gttgtgatgt atgcatcggg
      661 cgtttctatc tctctccgtg agcaacgagc tagtcaaccc atcgatccat ttttgtgagc
      721 acacacagag agagactcaa acacactcac agcttcacac tgagctccaa gagctctgta
      781 gagagaagcc agagtatgct ttcctcgatt ttcctaacgt cttcttcttc tgcttttttc
      841 tttttcccaa cgagttgtag tccactaact tcaatttttc ctccagagaa agagttttag
      901 agagacagct gataaataca ctagagaaat aaaaagaatg ataaaaattt gctattttaa
      961 tcagttccct ctcgtttctc taagttttgt tgttgggttg ggaaattaaa attggaaaat
     1021 ggaaataacg tttttttctg tctaatcttg aattctccag acatttctta tttccttttt
     1081 tgttgcaagt ttttcttcct tctctaattt ttaatcaaag atatctcgct atgaactttg
     1141 aatttttgat attttgattc tataaataca taaagtaatc taaatgctct tttaaaataa
     1201 atctacagta gccctatttt cagggatccc cgggattggc caaaggaccc aaaggtatgt
     1261 ttcgaatgat actaacataa catagaacat tttcaggagg acccttggct agcgtcgacg
     1321 gtaccggtca gatatcactc gaggagctcc cgagatcttc agcgctcagc attcgtagaa
     1381 ttgtaagttt aaactttctc atactaacta acgcgctcta tttaaatttt cagaattcca
     1441 actgagcgcc ggtcgctacc attaccaact tgtctggtgt caaaaataat aggggccgct
     1501 gtcatcagag taagtttaaa ctgagttcta ctaactaacg agtaatattt aaattttcag
     1561 catctcgcgc ccgtgcctct gacttctaag tccaattact cttcaacatc cctacatgct
     1621 ctttctccct gtgctcccac cccctatttt tgttattatc aaaaaaactt cttcttaatt
     1681 tctttgtttt ttagcttctt ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa
     1741 tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc attaataata
     1801 attctatccc aaaatctaca caatgttctg tgtacacttc ttatgttttt tttacttctg
     1861 ataaattttt tttgaaacat catagaaaaa accgcacaca aaatacctta tcatatgtta
     1921 cgtttcagtt tatgaccgca atttttattt cttcgcacgt ctgggcctct catgacgtca
     1981 aatcatgctc atcgtgaaaa agttttggag tatttttgga atttttcaat caagtgaaag
     2041 tttatgaaat taattttcct gcttttgctt tttgggggtt tcccctattg tttgtcaaga
     2101 gtttcgagga cggcgttttt cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa
     2161 agatcggaag aaggtttggg tttgaggctc agtggaaggt gagtagaagt tgataatttg
     2221 aaagtggagt agtgtctatg gggtttttgc cttaaatgac agaatacatt cccaatatac
     2281 caaacataac tgtttcctac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt
     2341 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     2401 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     2461 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     2521 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcggcctta agggcctcgt
     2581 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     2641 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2701 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2761 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2821 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2881 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2941 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3001 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3061 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3121 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3181 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3241 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3301 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3361 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3421 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3481 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3541 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3601 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3661 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     3721 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3781 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3841 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3901 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     3961 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4021 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4081 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4141 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc
     4201 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4261 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4321 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4381 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4441 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4501 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     4561 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     4621 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     4681 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     4741 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     4801 gctgtaagtt taaacatgat cttactaact aactattctc atttaaattt tcagagctta
     4861 aaaatggctg aaatcactca caacgatgga tacgctaaca acttggaaat gaaat
//
