LOCUS       sequence-208603-        6923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses proteins in C. elegans cholinergic neurons in head, 
            ventral nerve cord, and tail.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Williams SL, Lutz S, Charlie NK, Vettel C, Ailion M, Coco C, Tesmer 
            JJ, Jorgensen EM, Wieland T, Miller KG
  TITLE     Trio's Rho-specific GEF domain is the missing Galpha q effector in 
            C. elegans.
  JOURNAL   Genes Dev. 2007 Nov 1;21(21):2731-46. doi: 10.1101/gad.1592007. Epub
            2007 Oct 17.
  PUBMED    17942708
REFERENCE   2  (bases 1 to 6923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.1592007"; journalName: "Genes Dev"; date: "2007-11-1- 
            1"; volume: "21"; issue: "21"; pages: "2731-46"
COMMENT     Sequence Label: KG#94
FEATURES             Location/Qualifiers
     source          1..6923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4363..4385)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4485..4504
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4582..4600)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4668..4772
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4773..4841,4842..5633)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4991..5010)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5804..6392
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6293..6312
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6546..6563
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6680..6701
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6716..6721,6722..6739,6740..6746)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6754..6770
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6759..6781
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6778..6794
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6778..6794
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagaaatttc caaaaaaaac gtatacacca atcatttctc ccctccgaaa tgtgtagtcg
       61 tcatgacaaa gtggtgacac tggattacgg taggctccgc ccacttcttg gaaaagtgac
      121 gtgggctcgg tgagccctaa agcttttggt tttcacaatt ttctggtttt tgggattttt
      181 gcgggaattt tgagaggaga aagttgggag agaagatatt ggaatggtcc ccggggagct
      241 gtacatttat acatctagac tgaagctggg gtattgcaaa aagagaaaag cctatcactt
      301 tctttccatc ctgcatactt gccaaaaagt tttggctgag cttattttta aaattggaat
      361 tttttggaat tttcagaaaa gaaaaagttt tttctttttt tttaatttaa aaaatttttg
      421 ctatttattg catggtacta cagaaaaact tgaatgaaat aaaaaatttg gaaaattcaa
      481 gaaaactttt tttttcatag tttatttatt cgagttttaa tgtggctttt attttgttcg
      541 gtttttgttt taaaaagatt ttatacaaaa tttcaagaat ttacaatttt tccgttgtaa
      601 aaaatccaaa aaaaaatcca aaaaaatagt tcagaccttt ttttaaattt taaatttaaa
      661 aattcaatac caaaaaatta aaaaaaatta gtttttccga gaataaagga aaaatgttga
      721 aaaaatatta tatataaaat ttccagaaaa tgtgaaaact ccaaaaaact gcagactttt
      781 ccccaaacta gctatttttt gaattcatta cctttaaagg tggagtaccg aaatttaaga
      841 ctttgctttt ttagacccaa aatggtccaa aactaccaaa tttcgtaatg agacgttctg
      901 aaaatttttc aaaaaaatgt tatgggagct caaagttttt gaaaaaatgg tatattttta
      961 gctaatttta accacttctt ggcgtcgcag cggttggaac tcaattttaa ttaaattatc
     1021 atccttcaat gtccttcaat ctttgcaatt tcaatcgacc atacgcgttc agagcatttt
     1081 tttttttcgt ttttcctccc gtccagcaca tatttaaata ttctctctcc ccctcccctg
     1141 gttcctaaaa acttgggaaa ggggaatttc gaaggaggtg gaagcttctt cttctccccg
     1201 agcacacaca cacatcctct cttcaaaatc tttaagaaat agtgcgtgtg aatgactaga
     1261 cgctgaagct aacttggttt ccgcgaacaa tcccaatcag tgcctgtgcc gaggcttcgg
     1321 agaagaagag attgacgtcg tctttttctt tttttgtgaa acagaataaa aaagaatacg
     1381 aaatggactt tttcacacat tttcgttact tttccagaaa aaaacgaagc tctagccatg
     1441 gaagtttgaa ttgaaaacct aaaccccgat gtgtattcat ttgagcttaa gcctaagcct
     1501 aattctacgc ctaagcataa gcctaagcct atgttcaagc ctaagccaaa ttgtagaacg
     1561 ttaccaaaag atcaaaccta tttttcctac agaaaaggtc taatgataac tgttaagcga
     1621 gagacaagga gagatatata tcaaaaacta gcccaaactg tgattaacat ctattaactg
     1681 acctcaaaaa aaaaaatagt agagattaaa tactgctcaa aaagtttctt ggaaaagtaa
     1741 attgttaggt ttctggggcc tgtaaaactg gaccgagttt tgaagaattt ttttaacttt
     1801 tcaaagtttg gcgtcactga aagctttata ttgtacatgt tccaccaatt ttcttttgta
     1861 tccgtttttt ctgtctcaaa acggagctcg agcccatgac gtgtggatac cgtgagaatc
     1921 cgcccaattc cttcacatcg atctcatatt tctttcccag tttatattga attcccgttg
     1981 aattgcggct ttgcagggga gaagatgtgc gtctttatat ttttttttct atccttaccc
     2041 gccgtgaatc cggctcgaat tgagccaatt tttatgaata gtagagctaa ttgacttcgt
     2101 ctccctagaa ttgatgaggt aaagctcgtg gggagatatg gagatttttc gatttttttt
     2161 ccagtaattt tcagttcaaa atttgagctt ttgagcagga gttttgagat ttgtgccaaa
     2221 atttcctatt attttttcaa atatttttgc ttttgtaaaa aaggtgtagt agaagttttg
     2281 tagtgccact attttttttt tgaaaattat gttttggcgg aaagttcttt aaaaaagtta
     2341 atcaaaattt ttggcgcttt agtcaaatta attttctggc aaaaatatta gtaattttgg
     2401 cgtgagttca attttcccat tcttaaaatt tcacacaatt aagaatttta agatttgggt
     2461 ttttttttca acaaaaaatc aaatatttac atgaaaattc ggcgggaaat aaaaaaaaat
     2521 attccgattc aatgtgtgaa taactttttg cggggaattc aaatttgcat tatttaaact
     2581 ttttttaagc tcgaaaaatt gaaattctta aataaataaa taaaattttc ctcgaatttt
     2641 gaatgtttcg attttttagc gttatttttt caaaatttcc actaaaactc tagttctaaa
     2701 gttctaaaac tttaaaaact tcctaatgtt gtggtcttgt gtttttttgg cagcgatttc
     2761 caaatttctg aaattccaac aaaaaaacca ctacgtcagt ttgcatatcc aacccccccc
     2821 cccccccccc cccctctgag ccaatcaatc atgtgcattt tgaattggcg ccaaggcaat
     2881 cttcgtaaaa aataacaata gagtcatccc tcagaacaga aaagaaaccc gcccgggggc
     2941 tcaaatacac atgtcatttt tcttttttcg cctgattttt agcctaaatt agtgtttagc
     3001 aattttttaa ggtaactcgg tggagtcatt tgaacttttt aaactaaaat ttcagaaaaa
     3061 attctttttt tttgtcaaaa aataattttc ccgccggaat ttttcaaaaa aataattttt
     3121 attttatttt tcaagttcct tgtttcctct ttattttttt attttgcccc caaaagtttt
     3181 acactaaaat atttaatttt caggtaattc atcgaaagtc tttctatttt ccgcatctct
     3241 tgttcaacca aaggacccaa aggtatgttt cgaatgatac taacataaca tagaacattt
     3301 tcaggaggac ccttggctag cgtcgacggt accggtcaga tatcactcga ggagctcccg
     3361 agatcttcag cgctcagcat tcgtagaatt gtaagtttaa actttctcat actaactaac
     3421 gcgctctatt taaattttca gaattccaac tgagcgccgg tcgctaccat taccaacttg
     3481 tctggtgtca aaaataatag gggccgctgt catcagagta agtttaaact gagttctact
     3541 aactaacgag taatatttaa attttcagca tctcgcgccc gtgcctctga cttctaagtc
     3601 caattactct tcaacatccc tacatgctct ttctccctgt gctcccaccc cctatttttg
     3661 ttattatcaa aaaaacttct tcttaatttc tttgtttttt agcttctttt aagtcacctc
     3721 taacaatgaa attgtgtaga ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa
     3781 attgtgctcc ctccccccat taataataat tctatcccaa aatctacaca atgttctgtg
     3841 tacacttctt atgttttttt tacttctgat aaattttttt tgaaacatca tagaaaaaac
     3901 cgcacacaaa ataccttatc atatgttacg tttcagttta tgaccgcaat ttttatttct
     3961 tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat cgtgaaaaag ttttggagta
     4021 tttttggaat ttttcaatca agtgaaagtt tatgaaatta attttcctgc ttttgctttt
     4081 tgggggtttc ccctattgtt tgtcaagagt ttcgaggacg gcgtttttct tgctaaaatc
     4141 acaagtattg atgagcacga tgcaagaaag atcggaagaa ggtttgggtt tgaggctcag
     4201 tggaaggtga gtagaagttg ataatttgaa agtggagtag tgtctatggg gtttttgcct
     4261 taaatgacag aatacattcc caatatacca aacataactg tttcctacta gtcggccgta
     4321 cgggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     4381 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     4441 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     4501 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     4561 ccgcatcagg cggccttaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     4621 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4681 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4741 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4801 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4861 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4921 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4981 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5041 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5101 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5161 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5221 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5281 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5341 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5401 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5461 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5521 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5581 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     5641 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5701 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5761 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5821 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5881 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5941 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6001 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6061 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6121 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6181 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6241 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6301 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6361 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6421 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6481 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     6541 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     6601 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     6661 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     6721 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     6781 gaaacagcta tgaccatgat tacgccaagc tgtaagttta aacatgatct tactaactaa
     6841 ctattctcat ttaaattttc agagcttaaa aatggctgaa atcactcaca acgatggata
     6901 cgctaacaac ttggaaatga aat
//
