LOCUS       sequence-208617-       10458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pUDP123 expressing a polycistronic g-RNA array for Cas9 editing 
            targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. 
            parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10458)
  AUTHORS   Juergens H, Varela JA, Gorter de Vries AR, Perli T, Gast VJM, 
            Gyurchev NY, Rajkumar AS, Mans R, Pronk JT, Morrissey JP, Daran JG
  TITLE     Genome editing in Kluyveromyces and Ogataea yeasts using a 
            broad-host-range Cas9/gRNA co-expression plasmid.
  JOURNAL   FEMS Yeast Res. 2018 May 1;18(3). pii: 4847887. doi: 
            10.1093/femsyr/foy012.
  PUBMED    29438517
REFERENCE   2  (bases 1 to 10458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2018-05-1"; volume: "18(3). pii: 4847887. doi"; pages:
            " 10.1093/femsyr/foy012"
COMMENT     Sequence Label: pUDP123
FEATURES             Location/Qualifiers
     source          1..10458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(38..56)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(join(490..1281,1282..1350))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1113..1132
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1351..1455)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1558..2146
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2047..2066
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     gene            2310..3887
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     CDS             2658..3686
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             2658..3686
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             4265..4288
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4292..4312
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4313..8413
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTYKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSITHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8417..8437
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        9356..10022
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     9892..9915
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     ncRNA           10029..10065
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        10086..10161
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           10166..10233
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     ncRNA           10250..10286
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        10307..10382
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           10387..10454
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
ORIGIN
        1 gccccttttc ctttgtcgat atcatgtaat tagttatgtc acgcttacat tcacgccctc
       61 cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc taggtcccta
      121 tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa atttttcttt
      181 tttttctgta caaacgcgtg tacgcatgta acattatact gaaaaccttg cttgagaagg
      241 ttttgggaca agcttacgtc tcacggatcg tatatgccgt agcgacaatc taagaactat
      301 gcgaggacac gctagcagct gtattcacgt agacggatag gtatagccag acatcagcag
      361 catacttcgg gaaccgtagg ccaccgagcg gccgcgatta tcaaaaagga tcttcaccta
      421 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
      481 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
      541 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
      601 atctggcccc agtgctgcaa tgataccgcg ggacccacgc tcaccggctc cagatttatc
      661 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
      721 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
      781 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
      841 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
      901 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
      961 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     1021 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     1081 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     1141 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     1201 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     1261 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     1321 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     1381 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     1441 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gtcatgacca aaatccctta
     1501 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1561 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1621 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1681 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1741 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1801 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1861 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1921 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     1981 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2041 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2101 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2161 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2221 atcccctgat tctgtggata accgtgcgga ctatatgtga aggcatggct atggcacggc
     2281 agacattccg ccagatcatc aataggcacg acatggaggc ccagaatacc ctccttgaca
     2341 gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt tagcccatac
     2401 atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg cgaagcaaaa
     2461 attacggctc ctcgctgcag acctgcgagc agggaaacgc tcccctcaca gacgcgttga
     2521 attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg ccactgaggt
     2581 tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac atcacatccg
     2641 aacataaaca aggatccatg ggtaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     2701 ttctgatcga aaagttcgac agcgtgtccg acctgatgca gctctcggag ggcgaagaat
     2761 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     2821 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     2881 ttccggaagt gcttgacatt ggggaattta gcgagagcct gacctattgc atctcccgcc
     2941 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcaac
     3001 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     3061 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     3121 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     3181 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     3241 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     3301 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     3361 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     3421 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     3481 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     3541 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     3601 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     3661 gcactcgtcc gagggcaaag gaataactcg aggacaataa aaagattctt gttttcaaga
     3721 acttgtcatt tgtatagttt ttttatattg tagttgttct attttaatca aatgttagcg
     3781 tgatttatat tttttttcgc ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga
     3841 aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc tatactggag ctctcgagaa
     3901 ccctcacctt tcgagaggac gatgcccgtg tctaaatgat tcgaccagcc taagaatgtt
     3961 caacaatcta taatcagtcc atagtcaaca agagccctca aagttgatat cgtttagtga
     4021 tctccctcga gatgagcgga ggatggttgc tttttgggga gccctaagtg gtacgtgcgg
     4081 cgagcgggat gtttccccat taggcaaagg acgaccgggt aacccctcga gaaaaaaaat
     4141 ttttcgccgc taatctggtg ttatataaag ctccccctgc tcctggaatt tttttccttg
     4201 tcaactcaca ccggaaatca aggcatttca ttctgagtag ttcttaaaaa cataatcaac
     4261 aatggattat aaagatgacg atgacaaacc tccaaaaaag aagagaaagg tcgataagaa
     4321 atactcaata ggcttagata tcggcacaaa tagcgtcgga tgggcggtga tcactgatga
     4381 atataaggtt ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa
     4441 aaaaaatctt ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct
     4501 caaacggaca gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga
     4561 gattttttca aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc
     4621 ttttttggtg gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga
     4681 tgaagttgct tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga
     4741 ttctacttat aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt
     4801 tcgtggtcat tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact
     4861 atttatccag ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag
     4921 tggagtagat gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa
     4981 tctcattgct cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt
     5041 gtcattgggt ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt
     5101 acagctttca aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga
     5161 tcaatatgct gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga
     5221 tatcctaaga gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg
     5281 ctacgatgaa catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc
     5341 agaaaagtat aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga
     5401 tgggggagct agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga
     5461 tggtactgag gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac
     5521 ctttgacaac ggctctatta cccatcaaat tcacttgggt gagctgcatg ctattttgag
     5581 aagacaagaa gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt
     5641 gacttttcga attccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg
     5701 gatgactcgg aagtctgaag aaacaattac cccatggaat tttgaagaag ttgtcgataa
     5761 aggtgcttca gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa
     5821 tgaaaaagta ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt
     5881 gacaaaggtc aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca
     5941 gaagaaagcc attgttgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt
     6001 aaaagaagat tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga
     6061 agatagattt aatgcttcat taggtaccta ccatgatttg ctaaaaatta ttaaagataa
     6121 agattttttg gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac
     6181 cttatttgaa gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga
     6241 tgataaggtg atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg
     6301 aaaattgatt aatggtatta gggataagca atctggcaaa acaatattag attttttgaa
     6361 atcagatggt tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt
     6421 taaagaagac attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat
     6481 tgcaaattta gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt
     6541 tgatgaattg gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc
     6601 acgtgaaaat cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat
     6661 cgaagaaggt atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac
     6721 tcaattgcaa aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt
     6781 ggaccaagaa ttagatatta atcgtttaag tgattatgat gtcgatcaca ttgttccaca
     6841 aagtttcctt aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg
     6901 tggtaaatcg gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag
     6961 acaacttcta aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga
     7021 acgtggaggt ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac
     7081 tcgccaaatc actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga
     7141 tgaaaatgat aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc
     7201 tgacttccga aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc
     7261 ccatgatgcg tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact
     7321 tgaatcggag tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa
     7381 gtctgagcaa gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa
     7441 cttcttcaaa acagaaatta cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga
     7501 aactaatggg gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg
     7561 caaagtattg tccatgcccc aagtcaatat tgtcaagaaa acagaagtac agacaggcgg
     7621 attctccaag gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa
     7681 agactgggat ccaaaaaaat atggtggttt tgatagtcca acggtagctt attcagtcct
     7741 agtggttgct aaggtggaaa aagggaaatc gaagaagtta aaatccgtta aagagttact
     7801 agggatcaca attatggaaa gaagttcctt tgaaaaaaat ccgattgact ttttagaagc
     7861 taaaggatat aaggaagtta aaaaagactt aatcattaaa ctacctaaat atagtctttt
     7921 tgagttagaa aacggtcgta aacggatgct ggctagtgcc ggagaattac aaaaaggaaa
     7981 tgagctggct ctgccaagca aatatgtgaa ttttttatat ttagctagtc attatgaaaa
     8041 gttgaagggt agtccagaag ataacgaaca aaaacaattg tttgtggagc agcataagca
     8101 ttatttagat gagattattg agcaaatcag tgaattttct aagcgtgtta ttttagcaga
     8161 tgccaattta gataaagttc ttagtgcata taacaaacat agagacaaac caatacgtga
     8221 acaagcagaa aatattattc atttatttac gttgacgaat cttggagctc ccgctgcttt
     8281 taaatatttt gatacaacaa ttgatcgtaa acgatatacg tctacaaaag aagttttaga
     8341 tgccactctt atccatcaat ccatcactgg tctttatgaa acacgcattg atttgagtca
     8401 gctaggaggt gaccctccaa aaaagaagag aaaggtctga gcggttttgt ataactaaat
     8461 aatattggaa actaaatacg aatacccaaa ttttttatct aaattttgcc gaaagattaa
     8521 aatctgcaga gatatccgaa acaggtaaat ggatgtttca atccctgtag tcagtcagga
     8581 acccatatta tattacagta ttagtcgccg cttaggcacg cctttaatta gcaaaatcaa
     8641 accttaagtg catatgccgt ataagggaaa ctcaaagaac tggcatcgca aaaatgaaaa
     8701 catacgttga aactacggca aaggattggt cagatcgctt catacaggga aagttcggca
     8761 tcaacatctt tggataatat cagaatgaga aagaacagat acgcagtacg ttttttggtg
     8821 agctctttgc acttctttag ttctttccat caatatcagt tttttaaact tttaggacta
     8881 aaagtgatgt ttaacttcaa aatgtttaaa attttgttct tcccgacgtt cattaagaat
     8941 actaatacac tttaataatt attttaatat tttgttctaa ataatgactt ttaattaaaa
     9001 aagataaaat ataaaaacat cataataact caccagaggt taagaacaaa aaaacaaatt
     9061 agatatctgc taatccaata tagttaaatc aatctttcct tggtataatg ggtatattac
     9121 atatatttca aggaccgaca ctcctaccaa atatctaaaa tttaccatat taacataaca
     9181 tgtatataaa cgtcaaatca taatcagcac taacgtctca cggatcgtat atgccgtagc
     9241 gacaatctaa gaactatgcg aggacacgct agggccaagg caaaaagatt ccttgattac
     9301 gtaagggagt tagaatcatt ttgaataaaa aacacgcttt ttcagttcga gtttatcatt
     9361 atcaatactg ccatttcaaa gaatacgtaa ataattaata gtagtgattt tcctaacttt
     9421 atttagtcaa aaaattagcc ttttaattct gctgtaaccc gtacatgccc aaaatagggg
     9481 gcgggttaca cagaatatat aacatcgtag gtgtctgggt gaacagttta ttcctggcat
     9541 ccactaaata taatggagcc cgctttttaa gctggcatcc agaaaaaaaa agaatcccag
     9601 caccaaaata ttgttttctt caccaaccat cagttcatag gtccattctc ttagcgcaac
     9661 tacagagaac aggggcacaa acaggcaaaa aacgggcaca acctcaatgg agtgatgcaa
     9721 cctgcctgga gtaaatgatg acacaaggca attgacccac gcatgtatct atctcatttt
     9781 cttacacctt ctattacctt ctgctctctc tgatttggaa aaagctgaaa aaaaaggttg
     9841 aaaccagttc cctgaaatta ttcccctact tgactaataa gtatataaag acggtaggta
     9901 ttgattgtaa ttctgtaaat ctatttctta aacttcttaa attctacttt tatagttagt
     9961 ctttttttta gttttaaaac accaagaact tagtttcgaa taaacacaca taaacaaaca
    10021 aattccagct gatgagtccg tgaggacgaa acgagtaagc tcgtcctgga attgatctgc
    10081 ttggcgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
    10141 aaagtggcac cgagtcggtg cttttggccg gcatggtccc agcctcctcg ctggcgccgg
    10201 ctgggcaaca tgcttcggca tggcgaatgg gaccatcgtt ggctgtgctc tgatgagtcc
    10261 gtgaggacga aacgagtaag ctcgtcagca cagaccatag taactggttt tagagctaga
    10321 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
    10381 gcttttggcc ggcatggtcc cagcctcctc gctggcgccg gctgggcaac atgcttcggc
    10441 atggcgaatg ggacacag
//
