LOCUS       sequence-208620-        4198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses proteins in cholinergic neurons in the ventral nerve cord 
            of C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Charlie NK, Schade MA, Thomure AM, Miller KG
  TITLE     Presynaptic UNC-31 (CAPS) is required to activate the G alpha(s) 
            pathway of the Caenorhabditis elegans synaptic signaling network.
  JOURNAL   Genetics. 2006 Feb;172(2):943-61. doi: 10.1534/genetics.105.049577. 
            Epub 2005 Nov 4.
  PUBMED    16272411
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.105.049577"; journalName: "Genetics"; date: 
            "2006-02"; volume: "172"; issue: "2"; pages: "943-61"
COMMENT     Sequence Label: KG#65
FEATURES             Location/Qualifiers
     source          1..4198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1638..1660)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1760..1779
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1857..1875)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1943..2047
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2048..2116,2117..2908)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2266..2285)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3079..3667
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3568..3587
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3821..3838
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3955..3976
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3991..3996,3997..4014,4015..4021)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4029..4045
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4034..4056
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4053..4069
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4053..4069
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagaagct tttggttttc acaattttct ggtttttggg atttttgcgg
       61 gaattttgag aggagaaagt tgggagagaa gatattggaa tggtccccgg ggagctgtac
      121 atttatacac caaggcaatc ttcgtaaaaa ataacaatag agtcatccct cagaacagaa
      181 aagaaacccg cccgggggct caaatacaca tgtcattttt cttttttcgc ctgattttta
      241 gcctaaatta gtgtttagca attttttaag gtaactcggt ggagtcattt gaacttttta
      301 aactaaaatt tcagaaaaaa ttcttttttt ttgtcaaaaa ataattttcc cgccggaatt
      361 tttcaaaaaa ataattttta ttttattttt caagttcctt gtttcctctt tattttttta
      421 ttttgccccc aaaagtttta cactaaaata tttaattttc aggtaattca tcgaaagtct
      481 ttctattttc cgcatctctt gttcaaggat ccccgggatt ggccaaagga cccaaaggta
      541 tgtttcgaat gatactaaca taacatagaa cattttcagg aggacccttg gctagcgtcg
      601 acggtaccgg tcagatatca ctcgaggagc tcccgagatc ttcagcgctc agcattcgta
      661 gaattgtaag tttaaacttt ctcatactaa ctaacgcgct ctatttaaat tttcagaatt
      721 ccaactgagc gccggtcgct accattacca acttgtctgg tgtcaaaaat aataggggcc
      781 gctgtcatca gagtaagttt aaactgagtt ctactaacta acgagtaata tttaaatttt
      841 cagcatctcg cgcccgtgcc tctgacttct aagtccaatt actcttcaac atccctacat
      901 gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaaa cttcttctta
      961 atttctttgt tttttagctt cttttaagtc acctctaaca atgaaattgt gtagattcaa
     1021 aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata
     1081 ataattctat cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttttactt
     1141 ctgataaatt ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg
     1201 ttacgtttca gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg
     1261 tcaaatcatg ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga
     1321 aagtttatga aattaatttt cctgcttttg ctttttgggg gtttccccta ttgtttgtca
     1381 agagtttcga ggacggcgtt tttcttgcta aaatcacaag tattgatgag cacgatgcaa
     1441 gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga agttgataat
     1501 ttgaaagtgg agtagtgtct atggggtttt tgccttaaat gacagaatac attcccaata
     1561 taccaaacat aactgtttcc tactagtcgg ccgtacgggc cctttcgtct cgcgcgtttc
     1621 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     1681 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     1741 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     1801 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcggcc ttaagggcct
     1861 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     1921 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1981 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     2041 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     2101 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     2161 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     2221 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     2281 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     2341 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     2401 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     2461 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     2521 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     2581 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     2641 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     2701 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     2761 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     2821 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     2881 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     2941 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     3001 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     3061 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3121 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3181 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     3241 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3301 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3361 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3421 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag
     3481 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3541 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3601 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3661 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3721 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3781 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     3841 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     3901 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     3961 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     4021 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     4081 caagctgtaa gtttaaacat gatcttacta actaactatt ctcatttaaa ttttcagagc
     4141 ttaaaaatgg ctgaaatcac tcacaacgat ggatacgcta acaacttgga aatgaaat
//
