LOCUS       sequence-208639-         640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of E.coli DHFR N23PP mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Bhabha G, Lee J, Ekiert DC, Gam J, Wilson IA, Dyson HJ, Benkovic SJ,
            Wright PE
  TITLE     A dynamic knockout reveals that conformational fluctuations 
            influence the chemical step of enzyme catalysis.
  JOURNAL   Science. 2011 Apr 8;332(6026):234-8. doi: 10.1126/science.1198542.
  PUBMED    21474759
REFERENCE   2  (bases 1 to 640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-04-8- 8"; volume: "332"; issue: 
            "6026"; pages: "234-8"
COMMENT     Sequence Label: pET27b-ecDHFR-N23PP
FEATURES             Location/Qualifiers
     source          1..640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             572..604
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             611..628
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtttaacttt aagaaggaga tatacatatg atcagtctga ttgcggcgct agcggtagat
       61 cgcgttatcg gcatggaaaa cgccatgcca tggccgccgc tgcctgccga tctcgcctgg
      121 tttaaacgca acaccttaaa taaacccgtg attatggggc gccatacctg ggaatcaatc
      181 ggtaggcctt tgcccggccg caaaaatatt atcctcagca gtcaacccgg gaccgatgat
      241 cgggttacct gggttaaatc ggtcgacgaa gccatcgcgg cgtgtggtga cgtaccagaa
      301 atcatggtga ttggcggcgg acgcgtttat gaacagttct tgccaaaagc gcaaaaactg
      361 tatctgacgc atatcgatgc agaagtggaa ggcgacaccc attttccgga ttacgagccg
      421 gatgactggg aatcggtatt cagcgaattc cacgatgctg atgcgcagaa ctcgcatagc
      481 tattgtttcg aaatcctcga gcgtcgttaa ggatccgaat tcgagctccg tcgacaagct
      541 tgcggccgca ctcgagatca aacgggctag ccagccagaa ctcgccccgg aagaccccga
      601 ggatgtcgag caccaccacc accaccactg agatccggct
//
