LOCUS       sequence-208640-         640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of E.coli DHFR N23PP/S148A mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Bhabha G, Lee J, Ekiert DC, Gam J, Wilson IA, Dyson HJ, Benkovic SJ,
            Wright PE
  TITLE     A dynamic knockout reveals that conformational fluctuations 
            influence the chemical step of enzyme catalysis.
  JOURNAL   Science. 2011 Apr 8;332(6026):234-8. doi: 10.1126/science.1198542.
  PUBMED    21474759
REFERENCE   2  (bases 1 to 640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-04-8- 8"; volume: "332"; issue: 
            "6026"; pages: "234-8"
COMMENT     Sequence Label: pET27b-ecDHFR-N23PP/S148A
FEATURES             Location/Qualifiers
     source          1..640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             573..605
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             612..629
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgtttaactt taagaaggag atatacatat gatcagtctg attgcggcgc tagcggtaga
       61 tcgcgttatc ggcatggaaa acgccatgcc atggccgccg ctgcctgccg atctcgcctg
      121 gtttaaacgc aacaccttaa ataaacccgt gattatgggg cgccatacct gggaatcaat
      181 cggtaggcct ttgcccggcc gcaaaaatat tatcctcagc agtcaacccg ggaccgatga
      241 tcgggttacc tgggttaaat cggtcgacga agccatcgcg gcgtgtggtg acgtaccaga
      301 aatcatggtg attggcggcg gacgcgttta tgaacagttc ttgccaaaag cgcaaaaact
      361 gtatctgacg catatcgatg cagaagtgga aggcgacacc cattttccgg attacgagcc
      421 ggatgactgg gaatcggtat tcagcgaatt ccacgatgct gatgcgcaga acgcgcatag
      481 ctattgtttc gaaatcctcg agcgtcgtta aggatccgaa ttcgagctcc gtcgacaagc
      541 ttgcggccgc actcgagatc aaacgggcta gccagccaga actcgccccg gaagaccccg
      601 aggatgtcga gcaccaccac caccaccact gagatccggc
//
