LOCUS       sequence-208642-        6792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dynamin-like protein 1 fused to mTurquoise2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Pablo Rivera-Fuentes
  TITLE     mTurquoise2-labeled markers of mitophagy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pmTurquoise2-Drp1
FEATURES             Location/Qualifiers
     source          1..6792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        68..371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     526..546
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      614..623
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(620..622,623..625,626..1336)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(665..686)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(926..945)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1273..1294
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3587..3708
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3624..3643)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3678..3697
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3715..4170)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3852..3873)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4064..4083
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4197..4301
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4303..4660
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4303..4494
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4326..4346)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4511..4646
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4695..5489
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4695..5489
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4749..4768)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5359..5378
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5677..5696)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5721..5768
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6097..6685
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6586..6605
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
       61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      601 agcgctaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      661 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      721 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      781 gcccgtgccc tggcccaccc tcgtgaccac cctgtcctgg ggcgtgcagt gcttcgcccg
      841 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      901 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      961 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     1021 cggcaacatc ctggggcaca agctggagta caactacttt agcgacaacg tctatatcac
     1081 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
     1141 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     1201 gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga
     1261 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     1321 ggacgagctg tacaagtccg gactcagatc tcgagtgatg gaggcgctaa ttcctgtcat
     1381 aaacaagctc caggacgtct tcaacacggt gggcgccgac atcatccagc tgcctcaaat
     1441 cgtcgtagtg ggaacgcaga gcagcggaaa gagctccgtg ctagaaagcc tggtggggag
     1501 ggacctgctt cccagaggta ctggaattgt cacccggaga cctctcattc tgcaactggt
     1561 ccatgtttca caagaagata aacggaaaac aacaggagaa gaaaatggag tggaagcaga
     1621 agaatggggt aaatttcttc acaccaaaaa taagctttac acggattttg atgaaattcg
     1681 acaagaaatt gaaaatgaaa cagaaagaat ttcaggaaat aataagggag taagccctga
     1741 accaattcat cttaagattt tttcacccaa cgttgtcaat ttgacacttg tggatttgcc
     1801 aggaatgacc aaggtgcctg taggtgatca acctaaggat attgagcttc aaatcagaga
     1861 gctcattctt cggttcatca gtaatcctaa ttccattatc ctcgctgtca ctgctgctaa
     1921 tacagatatg gcaacatcag aggcacttaa aatttcaaga gaggtagatc cagatggtcg
     1981 cagaacccta gctgtaatca ctaaacttga tctcatggat gcgggtactg atgccatgga
     2041 tgtattgatg ggaagggtta ttccagtcaa acttggaata attggagtag ttaacaggag
     2101 ccagctagat attaacaaca agaagagtgt aactgattca atccgtgatg agtatgcttt
     2161 tcttcaaaag aaatatccat ctctggccaa tagaaatgga acaaagtatc ttgctaggac
     2221 tctaaacagg ttactgatgc atcacatcag agattgttta ccagagttga aaacgagaat
     2281 aaatgttcta gctgctcagt atcagtctct tctaaatagc tacggtgaac ccgtggatga
     2341 taaaagtgct actttactcc aacttattac caaatttgcc acagaatatt gtaacactat
     2401 tgaaggaact gcaaaatata ttgaaacttc ggagctatgc ggtggtgcta gaatttgtta
     2461 tattttccat gagacttttg ggcgaacctt agaatctgtt gatccacttg gtggccttaa
     2521 cactattgac attttgactg ccattagaaa tgctactggt cctcgtcctg ctttatttgt
     2581 gcctgaggtt tcatttgagt tactggtgaa gcggcaaatc aaacgtctag aagagcccag
     2641 cctccgctgt gtggaactgg ttcatgagga aatgcaaagg atcattcagc actgtagcaa
     2701 ttacagtaca caggaattgt tacgatttcc taaacttcat gatgccatag ttgaagtggt
     2761 gacttgtctt cttcgtaaaa ggttgcctgt tacaaatgaa atggtccata acttagtggc
     2821 aattgaactg gcttatatca acacaaaaca tccagacttt gctgatgctt gtgggctaat
     2881 gaacaataat atagaggaac aaaggagaaa caggctagcc agagaattac cttcagctgt
     2941 atcacgagac aaggttgcat ctggaggtgg tggggttgga gatggtgttc aagaaccaac
     3001 cacaggcaac tggagaggaa tgctgaaaac ttcaaaagct gaagagttat tagcagaaga
     3061 aaaatcaaaa cccattccaa ttatgccagc cagtccacaa aaaggtcatg ccgtgaacct
     3121 gctagatgtg ccagttcctg ttgcacgaaa actatctgct cgggaacagc gagattgtga
     3181 ggttattgaa cgactcatta aatcatattt tctcattgtc agaaagaata ttcaagacag
     3241 tgtgccaaag gcagtaatgc attttttggt taatcatgtg aaagacactc ttcagagtga
     3301 gctagtaggc cagctgtata aatcatcctt attggatgat cttctgacag aatctgagga
     3361 catggcacag cgcaggaaag aagcagctga tatgctaaag gcattacaag gagccagtca
     3421 aattattgct gaaatccggg agactcatct ttggtgagga tccaccggat ctagataact
     3481 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     3541 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     3601 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     3661 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg
     3721 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     3781 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     3841 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     3901 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     3961 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     4021 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     4081 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     4141 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
     4201 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4261 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca
     4321 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     4381 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     4441 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     4501 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4561 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4621 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
     4681 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     4741 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4801 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4861 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4921 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4981 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     5041 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     5101 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     5161 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     5221 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     5281 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     5341 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     5401 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     5461 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     5521 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     5581 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     5641 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
     5701 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
     5761 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
     5821 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
     5881 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
     5941 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6001 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6061 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6121 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6181 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6241 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtat caccgcctac
     6301 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6361 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6421 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6481 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6541 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6601 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6661 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6721 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6781 ccgtattacc gc
//
