LOCUS       sequence-208645-        8561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of full length TrkA fused to mCherry and 
            CRY2PHR (aa 1-498).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8561)
  AUTHORS   Duan L, Hope JM, Guo S, Ong Q, Francois A, Kaplan L, Scherrer G, Cui
            B
  TITLE     Optical Activation of TrkA Signaling.
  JOURNAL   ACS Synth Biol. 2018 Jul 20;7(7):1685-1693. doi: 
            10.1021/acssynbio.8b00126. Epub 2018 Jul 12.
  PUBMED    29975841
REFERENCE   2  (bases 1 to 8561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00126"; journalName: "ACS Synth Biol"; date: 
            "2018-07-20- 20"; volume: "7"; issue: "7"; pages: "1685-1693"
COMMENT     Sequence Label: TrkA-mCherry-CRY2
FEATURES             Location/Qualifiers
     source          1..8561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             667..693
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             673..699
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             706..750
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     regulatory      3058..3067
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3064..3771
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3209..3227)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3477..3496
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3778..5271
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     polyA_signal    5289..5410
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5326..5345)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5380..5399
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5417..5872)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5554..5575)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5766..5785
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5899..6003
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6005..6362
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6005..6196
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6028..6048)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6213..6348
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6397..7191
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6397..7191
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6451..6470)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7061..7080
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7379..7398)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7423..7470
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7799..8387
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8288..8307
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgaggc caccatgctg cgaggccagc ggcacgggca gctgggttgg
      601 catcgcccgg ccgcggggct aggcggtctg gtgacttcgt tgatgctggc ttgtgcttgc
      661 gccgcatacc catacgatgt tccagattac gctagcttgg gtggcggtct gaacgacatc
      721 ttcgaggctc agaaaatcga atggcacgaa ccctcctgtc gtgagacctg ctgtcccgtg
      781 ggcccctcgg ggttgcgctg caccagggca gggaccctga ataccctccg cggcctgcgg
      841 ggcgccggga acctgacgga gctctatgtg gaaaaccagc gggatctgca acgcctggag
      901 tttgaggacc tgcagggcct gggggagttg agaagcctaa ccatcgtgaa gagtggcctc
      961 cgctttgtgg cccctgatgc cttccatttc acccctcggc tcagtcacct gaatctctcc
     1021 tccaatgctt tggagtccct ctcctggaaa actgtgcagg gcctctccct acaggacttg
     1081 accctgtcag ggaacccact gcactgttcc tgtgccctat tgtggctcca gcgctgggag
     1141 caggaggatt tgtgtggtgt gtatacacaa aagcttcagg gctctgggtc tggagaccag
     1201 ttcctcccac tgggacacaa caacagttgt ggtgtaccct cagtgaagat ccagatgccc
     1261 aatgactctg tggaagtggg ggatgacgtt tttctgcagt gccaggtgga ggggcaggcc
     1321 ctacagcagg ctgactggat cctcacagag ctggaaggga cagccaccat gaagaaatct
     1381 ggagatctgc catccctggg gctaactctg gtcaatgtca ccagtgatct caacaagaag
     1441 aatgtgacgt gctgggcaga gaatgatgtg ggccgggctg aggtctctgt ccaagtcagc
     1501 gtctccttcc cagccagtgt gcatctgggc aaagccgtgg aacagcatca ctggtgcatt
     1561 cccttctctg tggacgggca gccagcaccg tccctgcgct ggttcttcaa cggctctgtg
     1621 ctcaatgaga ccagcttcat cttcactcag ttcttggagt cagcgctgac caatgagacc
     1681 atgcggcatg gctgccttcg cctcaaccag cccacgcatg tcaacaacgg gaactacacc
     1741 ctgctggctg ccaaccccta tggccaggct gctgcctcca tcatggctgc ctttatggac
     1801 aacccttttg agttcaaccc tgaggacccc atccctgtct ccttctcgcc agtggacact
     1861 aacagcacat caagagaccc agtggagaag aaggacgaaa caccttttgg ggtctctgtg
     1921 gctgtgggcc tggccgtctc cgccgccctc ttcctttctg ccctcctcct agtgctcaac
     1981 aaatgtggac agaggagcaa atttgggatc aaccgccctg ctgtgctggc gccagaggat
     2041 gggctggcca tgtccctaca cttcatgaca ctgggtggca gttctctttc ccctactgag
     2101 ggcaaaggct ccggactcca gggccacatc atggagaacc cacagtactt cagtgatacc
     2161 tgtgtccacc atatcaagcg ccaggacatc attctcaagt gggagctagg ggagggagcc
     2221 tttggaaagg tctttcttgc tgagtgctac aaccttctga atgatcagga caagatgctg
     2281 gtggctgtca aggcactgaa ggagacatct gagaatgctc gtcaggactt ccatcgtgag
     2341 gcagagctgc tcaccatgct acagcaccaa cacatcgtac gcttctttgg agtctgcacg
     2401 gagggtggcc cattgctcat ggtcttcgag tacatgcgcc atggggacct caaccgtttc
     2461 ctccggtccc acggacctga tgcaaaactg ctggctggcg gcgaggatgt ggctcctggt
     2521 cctttgggcc ttgggcagct tctggctgtg gctagccagg tggctgctgg tatggtgtac
     2581 ctagccagcc tgcactttgt gcaccgggac ctggccacac gcaactgtct ggtgggtcag
     2641 ggactagtgg tcaagattgg agacttcggc atgagcaggg acatctacag cacagactac
     2701 taccgtgtgg gaggtcggac catgctgccc atccgctgga tgcctccaga gagcatcctc
     2761 taccgcaagt tcagcaccga gagtgatgtg tggagcttcg gggtggtgct ctgggagatc
     2821 ttcacctatg gaaagcaacc ctggtaccag ctctccaaca ctgaggcgat cgagtgcatc
     2881 acgcagggcc gggagctgga gcggccgcgc gcctgccctc ctgatgtcta cgccatcatg
     2941 cgcggctgct ggcagcgtga gccgcaacag cgcctcagca tgaaggatgt gcacgcgcgg
     3001 ctgcaggcct tggcacaggc gccaccgagt tacctggacg ttctggatcc accggtcgcc
     3061 accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     3121 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     3181 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     3241 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     3301 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     3361 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     3421 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     3481 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     3541 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     3601 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     3661 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     3721 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gggatccatg
     3781 aagatggaca aaaagactat agtttggttt agaagagacc taaggattga ggataatcct
     3841 gcattagcag cagctgctca cgaaggatct gtttttcctg tcttcatttg gtgtcctgaa
     3901 gaagaaggac agttttatcc tggaagagct tcaagatggt ggatgaaaca atcacttgct
     3961 cacttatctc aatccttgaa ggctcttgga tctgacctca ctttaatcaa aacccacaac
     4021 acgatttcag cgatcttgga ttgtatccgc gttaccggtg ctacaaaagt cgtctttaac
     4081 cacctctatg atcctgtttc gttagttcgg gaccataccg taaaggagaa gctggtggaa
     4141 cgtgggatct ctgtgcaaag ctacaatgga gatctattgt atgaaccgtg ggagatatac
     4201 tgcgaaaagg gcaaaccttt tacgagtttc aattcttact ggaagaaatg cttagatatg
     4261 tcgattgaat ccgttatgct tcctcctcct tggcggttga tgccaataac tgcagcggct
     4321 gaagcgattt gggcgtgttc gattgaagaa ctagggctgg agaatgaggc cgagaaaccg
     4381 agcaatgcgt tgttaactag agcttggtct ccaggatgga gcaatgctga taagttacta
     4441 aatgagttca tcgagaagca gttgatagat tatgcaaaga acagcaagaa agttgttggg
     4501 aattctactt cactactttc tccgtatctc catttcgggg aaataagcgt cagacacgtt
     4561 ttccagtgtg cccggatgaa acaaattata tgggcaagag ataagaacag tgaaggagaa
     4621 gaaagtgcag atctttttct taggggaatc ggtttaagag agtattctcg gtatatatgt
     4681 ttcaacttcc cgtttactca cgagcaatcg ttgttgagtc atcttcggtt tttcccttgg
     4741 gatgctgatg ttgataagtt caaggcctgg agacaaggca ggaccggtta tccgttggtg
     4801 gatgccggaa tgagagagct ttgggctacc ggatggatgc ataacagaat aagagtgatt
     4861 gtttcaagct ttgctgtgaa gtttcttctc cttccatgga aatggggaat gaagtatttc
     4921 tgggatacac ttttggatgc tgatttggaa tgtgacatcc ttggctggca gtatatctct
     4981 gggagtatcc ccgatggcca cgagcttgat cgcttggaca atcccgcgtt acaaggcgcc
     5041 aaatatgacc cagaaggtga gtacataagg caatggcttc ccgagcttgc gagattgcca
     5101 actgaatgga tccatcatcc atgggacgct cctttaaccg tactcaaagc ttctggtgtg
     5161 gaactcggaa caaactatgc gaaacccatt gtagacatcg acacagctcg tgagctacta
     5221 gctaaagcta tttcaagaac ccgtgaagca cagatcatga tcggagcagc ataacaattg
     5281 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5341 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5401 atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     5461 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     5521 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     5581 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     5641 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     5701 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     5761 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     5821 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
     5881 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5941 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     6001 agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     6061 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     6121 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6181 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     6241 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     6301 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     6361 aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     6421 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     6481 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     6541 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     6601 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     6661 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     6721 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     6781 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     6841 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     6901 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     6961 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     7021 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     7081 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     7141 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     7201 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     7261 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     7321 tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg
     7381 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     7441 ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     7501 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     7561 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     7621 caggccctgc catagcctca ggttactcat atatacttta gattgattta aaacttcatt
     7681 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7741 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7801 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7861 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7921 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7981 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8041 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8101 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8161 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8221 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8281 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8341 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8401 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8461 tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca
     8521 attacggggt cattagttca tagcccatat atggagttcc g
//
