LOCUS       sequence-208654-       11383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses UBE3B fused to copGFP in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11383)
  AUTHORS   Braganza A, Li J, Zeng X, Yates NA, Dey NB, Andrews J, Clark J, 
            Zamani L, Wang XH, St Croix C, O'Sullivan R, Garcia-Exposito L, 
            Brodsky JL, Sobol RW
  TITLE     UBE3B is a Calmodulin-Regulated, Mitochondria-Associated E3 
            Ubiquitin Ligase.
  JOURNAL   J Biol Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 
            10.1074/jbc.M116.766824.
  PUBMED    28003368
REFERENCE   2  (bases 1 to 11383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 
            10.1074/jbc.M116.766824."
COMMENT     Sequence Label: pCT-CMV-UBE3B-copGFP-Puro
FEATURES             Location/Qualifiers
     source          1..11383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        12..238
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             239..419
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    466..591
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1084..1317
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1502..1546
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1813..1929
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2019..2222
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2173..2193
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2219..2243
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5570..6232
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     promoter        6343..6554
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6567..6835
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             6862..7461
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6862..6881)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7358..7378
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7468..8056
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7521..7541)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7939..7950)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8130..8363
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8435..8556
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8472..8491)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8526..8545
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8596..8731
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8764..8780)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8764..8780)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8777..8799)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8788..8804
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8812..8818,8819..8836,8837..8842))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8857..8878
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8995..9012)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9166..9754)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9925..10716,10717..10785))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10548..10567
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10786..10890)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10958..10976
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11014..11036)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11136..11155
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11349..11371
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11363..11380
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11364..11380
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagctgacgc gtgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
       61 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
      121 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
      181 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
      241 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
      301 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
      361 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
      421 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
      481 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
      541 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
      601 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
      661 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
      721 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
      781 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
      841 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
      901 aacaaaagta agaccaccgc acagcaagcg gccactgatc ttcagacctg gaggaggaga
      961 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1021 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1081 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1141 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1201 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1261 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1321 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1381 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     1441 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     1501 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     1561 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     1621 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     1681 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     1741 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     1801 gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     1861 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt
     1921 caaaatttta tcgatactag tattatgccc agtacatgac cttatgggac tttcctactt
     1981 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     2041 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     2101 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     2161 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtttat ataagcagag
     2221 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     2281 gaagattcta gacaccatgt tcaccctgtc tcagacctcg agagcatggt tcatcgatag
     2341 agcccgtcag gcacgagaag aaaggcttgt gcagaaggaa cgggagcggg cagctgttgt
     2401 gatccaggcc catgtccgga gttttctctg tcggagtcga ctgcagagag atatcaggag
     2461 agagattgat gactttttta aagcagatga ccctgagtcc actaaaagaa gtgcactttg
     2521 tattttcaag attgccagga aactgctgtt cctattcaga atcaaagagg ataatgagag
     2581 atttgagaag ttgtgtcgca gcatcctgag cagcatggat gctgagaatg agcctaaggt
     2641 gtggtatgtg tccctggctt gttctaagga cctcaccctc ctttggattc aacagatcaa
     2701 gaacattttg tggtactgct gtgattttct caagcagctc aagcctgaaa tcctgcagga
     2761 ctcccgactc atcaccctgt acctcacgat gcttgtcacc ttcacagaca cttcaacgtg
     2821 gaaaattctt cggggaaaag gtgaaagtct tcgaccagcg atgaaccaca tttgtgcaaa
     2881 tataatggga catctcaacc agcatggatt ttattctgtg ctgcagatat tgttaacccg
     2941 tggcctggca agaccccgtc cttgtctatc caaaggcact ttaacagcag ctttttctct
     3001 agcgttacgc cctgtgattg ctgcacagtt ctcagacaat ctgattcggc cgttcctcat
     3061 ccacatcatg tctgtgcctg ctctggtgac tcatctcagc acagtgaccc ctgagcgcct
     3121 cactgtttta gaatcccatg acatgcttcg taaattcatc atatttttaa gagaccaaga
     3181 tcgatgccgt gatgtatgtg aaagtttaga aggatgccat acgctttgtc taatgggcaa
     3241 cctcctacac ttgggctccc tcagccccag agtgttagag gaggagacag atgggttcgt
     3301 gagtttgctc acccagacgc tgtgctactg tcggaagtat gtgtctcaga agaagtccaa
     3361 cctgacccac tggcatcctg tccttggctg gttctcccaa tctgtggatt atggccttaa
     3421 cgagtcaatg cacttgatca ccaaacagct gcagttcttg tggggggtgc ctctgatccg
     3481 gatcttcttc tgtgacatcc tgagcaagaa gctactggag agccaggagc cagcccacgc
     3541 acagccagca tcccctcaga atgtgctccc agtgaagagt ctcctaaagc gtgcttttca
     3601 aaagtcggca tcagtccgga atattctcag gcctgtcggg ggtaaacggg tcgactctgc
     3661 agaagtccag aaggtttgca acatctgtgt cctctaccag acctcgctga caactctcac
     3721 acagattcgg ctgcagatac tcacaggtct cacttacctt gatgacctgc ttcccaaact
     3781 gtgggcattt atctgtgagc tcgggcccca cggagggtta aagctcttct tggaatgcct
     3841 gaacaatgac actgaagagt ccaagcaact cttggccatg ctgatgctgt tctgtgactg
     3901 ttcgcggcac ctcatcacaa tccttgatga cattgaagtt tatgaagaac agatttcatt
     3961 caaactggaa gagctggtca ctatctcctc tttcctgaat tcttttgtgt ttaagatgat
     4021 ctgggatgga attgtagaga acgccaaggg tgagaccttg gagctgttcc agtctgtcca
     4081 cgggtggctt atggtgctgt acgagcggga ctgccggcgg cgcttcaccc ccgaggacca
     4141 ctggctgcga aaggatctca aacctagcgt gctcttccaa gaactcgaca gggacagaaa
     4201 acgggcacag ttgatcctgc agtacatccc acatgtcatc cctcacaaaa acagagttct
     4261 actgtttcga accatggtta ccaaggagaa ggagaaactg gggctggtgg aaaccagctc
     4321 tgcctccccg catgtcactc acatcaccat ccgccggtcc aggatgctgg aggacggcta
     4381 cgagcagctt aggcagctct cccagcacgc catgaagggg gtcatccgtg tgaagtttgt
     4441 caatgacctc ggggtggacg aagcagggat tgatcaagac ggtgttttta aggagttctt
     4501 ggaagagatc atcaagagag tttttgaccc agcactcaat ctgttcaaga caaccagtgg
     4561 ggatgagagg ctgtacccct cacccacatc ctacatccat gagaattacc tgcagctctt
     4621 cgagtttgtg gggaagatgc tggggaaggc tgtgtatgag ggaattgtgg tggacgtgcc
     4681 atttgcatcc ttcttcctga gccaactgct tgggcaccac cacagcgtct tctatagctc
     4741 ggtggatgaa ctgccttctc tggactccga gttctataaa aacctcacct ccatcaagcg
     4801 ctatgatggg gacatcactg acctgggcct gacgctgtct tacgacgagg acgtcatggg
     4861 tcagcttgtt tgccatgaac tgattcctgg agggaagacc attcctgtta caaatgaaaa
     4921 taaaattagc tacatccatc tgatggcaca ttttcgaatg cacactcaaa taaaaaacca
     4981 aacagctgcc ctcattagcg gattccgttc cattatcaaa cccgagtgga tccgaatgtt
     5041 ctcaactcct gaactgcagc gtctcatctc tggcgacaat gctgagattg atctggaaga
     5101 tttaaagaag cacacagtct actacggtgg tttccatgga agtcacagag tcatcatctg
     5161 gctctgggat attctggcct ccgacttcac accggatgag agagctatgt ttctgaagtt
     5221 cgtgaccagc tgctccagac ccccgctcct gggattcgcc tacctcaagc ctccattctc
     5281 catccgctgc gtggaggtgt cggacgatca ggacaccggg gacactctgg gcagcgtcct
     5341 ccggggcttc ttcaccatcc gcaagcggga gccaggcggc cgcctgccca cctcctccac
     5401 ctgcttcaac ctgctcaagc tgcccaacta cagcaagaag agcgtcctcc gcgagaagct
     5461 gcgctacgcc atcagcatga acacgggctt tgaactctcc attctagagc tagcgaattc
     5521 gaatttaaat cggatccgcg gccgcatgga gagcgacgag agcggcctgc ccgccatgga
     5581 gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg
     5641 agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct
     5701 gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac
     5761 ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa
     5821 cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta
     5881 cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag
     5941 cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat
     6001 gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta
     6061 ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct
     6121 gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga
     6181 gctgggcatc gtggagtacc agcacgcctt caagaccccc attgccttcg ccagatcccg
     6241 cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg
     6301 ctaaggccgc aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc
     6361 ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt
     6421 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     6481 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     6541 ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc
     6601 ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg
     6661 cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt
     6721 gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc
     6781 tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc
     6841 tgtgaccggc gcctacgcta gatgaccgag tacaagccca cggtgcgcct cgccacccgc
     6901 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     6961 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     7021 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     7081 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     7141 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     7201 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     7261 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     7321 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     7381 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     7441 acccgcaagc ccggtgcctg agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     7501 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7561 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7621 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7681 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7741 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7801 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7861 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7921 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7981 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     8041 tgggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct
     8101 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagata
     8161 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     8221 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8281 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8341 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     8401 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     8461 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8521 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     8581 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8641 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     8701 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat
     8761 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     8821 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8881 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8941 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     9001 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     9061 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     9121 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     9181 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9241 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     9301 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     9361 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     9421 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     9481 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9541 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9601 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9661 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9721 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9781 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9841 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9901 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9961 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    10021 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    10081 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    10141 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    10201 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    10261 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    10321 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    10381 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    10441 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    10501 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10561 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10621 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10681 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10741 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10801 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10861 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10921 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
    10981 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
    11041 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    11101 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
    11161 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
    11221 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
    11281 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
    11341 agggttttcc cagtcacgac gttgtaaaac gacggccagt gcc
//
