LOCUS       sequence-208658-       10417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses UBE3B deletion of HECT domain fused to copGFP in mammalian
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10417)
  AUTHORS   Braganza A, Li J, Zeng X, Yates NA, Dey NB, Andrews J, Clark J, 
            Zamani L, Wang XH, St Croix C, O'Sullivan R, Garcia-Exposito L, 
            Brodsky JL, Sobol RW
  TITLE     UBE3B is a Calmodulin-Regulated, Mitochondria-Associated E3 
            Ubiquitin Ligase.
  JOURNAL   J Biol Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 
            10.1074/jbc.M116.766824.
  PUBMED    28003368
REFERENCE   2  (bases 1 to 10417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 
            10.1074/jbc.M116.766824."
COMMENT     Sequence Label: pCT-CMV-UBE3B-Delta-HECT-copGFP-Puro
FEATURES             Location/Qualifiers
     source          1..10417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..242
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             243..423
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    470..595
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1088..1321
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1506..1550
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1817..1933
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2023..2226
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2177..2197
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2223..2247
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4608..5270
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     promoter        5381..5592
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5605..5873
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5900..6499
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5900..5919)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6396..6416
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6506..7094
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6559..6579)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6977..6988)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7168..7401
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7473..7594
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7510..7529)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7564..7583
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7634..7769
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7802..7818)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7802..7818)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7815..7837)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7826..7842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7850..7856,7857..7874,7875..7880))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7895..7916
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8033..8050)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8204..8792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8963..9754,9755..9823))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9586..9605
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9824..9928)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9996..10014
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10052..10074)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10174..10193
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10387..10409
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(10401..10417,1)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     join(10402..10417,1)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgccaagctg acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
       61 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
      121 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
      181 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
      241 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
      301 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
      361 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
      421 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
      481 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
      541 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
      601 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
      661 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
      721 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
      781 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
      841 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
      901 gcaaaacaaa agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag
      961 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     1021 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     1081 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     1141 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     1201 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     1261 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     1321 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     1381 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     1441 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     1501 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     1561 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     1621 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     1681 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     1741 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
     1801 gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     1861 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     1921 aattcaaaat tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct
     1981 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2041 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2101 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2161 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc
     2221 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     2281 catagaagat tctagacacc atgttcaccc tgtctcagac ctcgagagca tggttcatcg
     2341 atagagcccg tcaggcacga gaagaaaggc ttgtgcagaa ggaacgggag cgggcagctg
     2401 ttgtgatcca ggcccatgtc cggagttttc tctgtcggag tcgactgcag agagatatca
     2461 ggagagagat tgatgacttt tttaaagcag atgaccctga gtccactaaa agaagtgcac
     2521 tttgtatttt caagattgcc aggaaactgc tgttcctatt cagaatcaaa gaggataatg
     2581 agagatttga gaagttgtgt cgcagcatcc tgagcagcat ggatgctgag aatgagccta
     2641 aggtgtggta tgtgtccctg gcttgttcta aggacctcac cctcctttgg attcaacaga
     2701 tcaagaacat tttgtggtac tgctgtgatt ttctcaagca gctcaagcct gaaatcctgc
     2761 aggactcccg actcatcacc ctgtacctca cgatgcttgt caccttcaca gacacttcaa
     2821 cgtggaaaat tcttcgggga aaaggtgaaa gtcttcgacc agcgatgaac cacatttgtg
     2881 caaatataat gggacatctc aaccagcatg gattttattc tgtgctgcag atattgttaa
     2941 cccgtggcct ggcaagaccc cgtccttgtc tatccaaagg cactttaaca gcagcttttt
     3001 ctctagcgtt acgccctgtg attgctgcac agttctcaga caatctgatt cggccgttcc
     3061 tcatccacat catgtctgtg cctgctctgg tgactcatct cagcacagtg acccctgagc
     3121 gcctcactgt tttagaatcc catgacatgc ttcgtaaatt catcatattt ttaagagacc
     3181 aagatcgatg ccgtgatgta tgtgaaagtt tagaaggatg ccatacgctt tgtctaatgg
     3241 gcaacctcct acacttgggc tccatcagcc ccagagtgtt agaggaggag acagatgggt
     3301 tcgtgagttt gctcacccag acgctgtgct actgtcggaa gtatgtgtct cagaagaagt
     3361 ccaacctgac ccactggcat cctgtccttg gctggttctc ccaatctgtg gattatggcc
     3421 ttaacgagtc aatgcacttg atcaccaaac agctgcagtt cttgtggggg gtgcctctga
     3481 tccggatctt cttctgtgac atcctgagca agaagctact ggagagccag gagccagccc
     3541 acgcacagcc agcatcccct cagaatgtgc tcccagtgaa gagtctccta aagcgtgctt
     3601 ttcaaaagtc ggcatcagtc cggaatattc tcaggcctgt cgggggtaaa cgggtcgact
     3661 ctgcagaagt ccagaaggtt tgcaacatct gtgtcctcta ccagacctcg ctgacaactc
     3721 tcacacagat tcggctgcag atactcacag gtctcactta ccttgatgac ctgcttccca
     3781 aactgtgggc atttatctgt gagctcgggc cccacggagg gttaaagctc ttcttggaat
     3841 gcctgaacaa tgacactgaa gagtccaagc aactcttggc catgctgatg ctgttctgtg
     3901 actgttcgcg gcacctcatc acaatccttg atgacattga agtttatgaa gaacagattt
     3961 cattcaaact ggaagagctg gtcactatct cctctttcct gaattctttt gtgtttaaga
     4021 tgatctggga tggaattgta gagaacgcca agggtgagac cttggagctg ttccagtctg
     4081 tccacgggtg gcttatggtg ctgtacgagc gggactgccg gcggcgcttc acccccgagg
     4141 accactggct gcgaaaggat ctcaaaccta gcgtgctctt ccaagaactc gacagggaca
     4201 gaaaacgggc acagttgatc ctgcagtaca tcccacatgt catccctcac aaaaacagag
     4261 ttctactgtt tcgaaccatg gttaccaagg agaaggagaa actggggctg gtggaaacca
     4321 gctctgcctc cccgcatgtc actcacatca ccatccgccg gtccaggatg ctggaggacg
     4381 gctacgagca gcttaggcag ctctcccagc acgccatgaa gggggtcatc cgtgtgaagt
     4441 ttgtcaatga cctcggggtg gacgaagcag ggattgatca agacggtgtt tttaaggagt
     4501 tcttggaaga gatcatcaag agagtttttg acccagcact caatctgttc aagacaacca
     4561 gtggggattg gatccgcggc cgcatggaga gcgacgagag cggcctgccc gccatggaga
     4621 tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg ggcggcggag
     4681 agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa ggcgccctga
     4741 ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac ttcggcacct
     4801 accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc tacaccaaca
     4861 cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc taccgctacg
     4921 aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc gaggacagcg
     4981 tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg caccccatgg
     5041 gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac ggcggctact
     5101 acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc agcatcctgc
     5161 agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc aacaccgagc
     5221 tgggcatcgt ggagtaccag cacgccttca agacccccat tgccttcgcc agatcccgcg
     5281 ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc ggatctcgct
     5341 aaggccgcaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     5401 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     5461 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     5521 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     5581 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     5641 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     5701 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     5761 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     5821 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     5881 tgaccggcgc ctacgctaga tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     5941 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     6001 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     6061 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     6121 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     6181 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     6241 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     6301 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     6361 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     6421 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     6481 ccgcaagccc ggtgcctgag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     6541 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6601 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6661 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6721 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6781 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6841 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6901 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6961 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7021 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7081 ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta
     7141 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagataag
     7201 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7261 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7321 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7381 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     7441 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     7501 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7561 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     7621 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7681 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     7741 gaagtagtga ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca
     7801 tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7861 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7921 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7981 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     8041 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8101 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8161 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8221 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8281 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8341 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8401 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8461 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8521 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8581 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8641 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8701 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8761 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8821 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8881 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8941 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9001 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9061 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9121 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9181 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9241 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9301 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9361 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9421 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9481 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9541 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9601 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9661 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9721 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9781 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9841 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9901 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     9961 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
    10021 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
    10081 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
    10141 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
    10201 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
    10261 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
    10321 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
    10381 ggttttccca gtcacgacgt tgtaaaacga cggccag
//
