LOCUS       sequence-208660-       11383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses UBE3B mutant C1036A fused to copGFP in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11383)
  AUTHORS   Braganza A, Li J, Zeng X, Yates NA, Dey NB, Andrews J, Clark J, 
            Zamani L, Wang XH, St Croix C, O'Sullivan R, Garcia-Exposito L, 
            Brodsky JL, Sobol RW
  TITLE     UBE3B is a Calmodulin-Regulated, Mitochondria-Associated E3 
            Ubiquitin Ligase.
  JOURNAL   J Biol Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 
            10.1074/jbc.M116.766824.
  PUBMED    28003368
REFERENCE   2  (bases 1 to 11383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 
            10.1074/jbc.M116.766824."
COMMENT     Sequence Label: pCT-CMV-UBE3B(C1036A)-copGFP-Puro
FEATURES             Location/Qualifiers
     source          1..11383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..235
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             236..416
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    463..588
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1081..1314
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1499..1543
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1810..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2016..2219
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2170..2190
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2216..2240
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5567..6229
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     promoter        6340..6551
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6564..6832
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             6859..7458
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6859..6878)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7355..7375
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7465..8053
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7518..7538)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7936..7947)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8127..8360
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8432..8553
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8469..8488)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8523..8542
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8593..8728
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8761..8777)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8761..8777)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8774..8796)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8785..8801
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8809..8815,8816..8833,8834..8839))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8854..8875
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8992..9009)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9163..9751)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9922..10713,10714..10782))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10545..10564
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10783..10887)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10955..10973
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11011..11033)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11133..11152
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11346..11368
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11360..11377
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11361..11377
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctgacgcgtg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
      901 aaaagtaaga ccaccgcaca gcaagcggcc actgatcttc agacctggag gaggagatat
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa
     1921 aattttatcg atactagtat tatgcccagt acatgacctt atgggacttt cctacttggc
     1981 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     2041 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     2101 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     2161 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtttatata agcagagctc
     2221 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
     2281 gattctagac accatgttca ccctgtctca gacctcgaga gcatggttca tcgatagagc
     2341 ccgtcaggca cgagaagaaa ggcttgtgca gaaggaacgg gagcgggcag ctgttgtgat
     2401 ccaggcccat gtccggagtt ttctctgtcg gagtcgactg cagagagata tcaggagaga
     2461 gattgatgac ttttttaaag cagatgaccc tgagtccact aaaagaagtg cactttgtat
     2521 tttcaagatt gccaggaaac tgctgttcct attcagaatc aaagaggata atgagagatt
     2581 tgagaagttg tgtcgcagca tcctgagcag catggatgct gagaatgagc ctaaggtgtg
     2641 gtatgtgtcc ctggcttgtt ctaaggacct caccctcctt tggattcaac agatcaagaa
     2701 cattttgtgg tactgctgtg attttctcaa gcagctcaag cctgaaatcc tgcaggactc
     2761 ccgactcatc accctgtacc tcacgatgct tgtcaccttc acagacactt caacgtggaa
     2821 aattcttcgg ggaaaaggtg aaagtcttcg accagcgatg aaccacattt gtgcaaatat
     2881 aatgggacat ctcaaccagc atggatttta ttctgtgctg cagatattgt taacccgtgg
     2941 cctggcaaga ccccgtcctt gtctatccaa aggcacttta acagcagctt tttctctagc
     3001 gttacgccct gtgattgctg cacagttctc agacaatctg attcggccgt tcctcatcca
     3061 catcatgtct gtgcctgctc tggtgactca tctcagcaca gtgacccctg agcgcctcac
     3121 tgttttagaa tcccatgaca tgcttcgtaa attcatcata tttttaagag accaagatcg
     3181 atgccgtgat gtatgtgaaa gtttagaagg atgccatacg ctttgtctaa tgggcaacct
     3241 cctacacttg ggctccctca gccccagagt gttagaggag gagacagatg ggttcgtgag
     3301 tttgctcacc cagacgctgt gctactgtcg gaagtatgtg tctcagaaga agtccaacct
     3361 gacccactgg catcctgtcc ttggctggtt ctcccaatct gtggattatg gccttaacga
     3421 gtcaatgcac ttgatcacca aacagctgca gttcttgtgg ggggtgcctc tgatccggat
     3481 cttcttctgt gacatcctga gcaagaagct actggagagc caggagccag cccacgcaca
     3541 gccagcatcc cctcagaatg tgctcccagt gaagagtctc ctaaagcgtg cttttcaaaa
     3601 gtcggcatca gtccggaata ttctcaggcc tgtcgggggt aaacgggtcg actctgcaga
     3661 agtccagaag gtttgcaaca tctgtgtcct ctaccagacc tcgctgacaa ctctcacaca
     3721 gattcggctg cagatactca caggtctcac ttaccttgat gacctgcttc ccaaactgtg
     3781 ggcatttatc tgtgagctcg ggccccacgg agggttaaag ctcttcttgg aatgcctgaa
     3841 caatgacact gaagagtcca agcaactctt ggccatgctg atgctgttct gtgactgttc
     3901 gcggcacctc atcacaatcc ttgatgacat tgaagtttat gaagaacaga tttcattcaa
     3961 actggaagag ctggtcacta tctcctcttt cctgaattct tttgtgttta agatgatctg
     4021 ggatggaatt gtagagaacg ccaagggtga gaccttggag ctgttccagt ctgtccacgg
     4081 gtggcttatg gtgctgtacg agcgggactg ccggcggcgc ttcacccccg aggaccactg
     4141 gctgcgaaag gatctcaaac ctagcgtgct cttccaagaa ctcgacaggg acagaaaacg
     4201 ggcacagttg atcctgcagt acatcccaca tgtcatccct cacaaaaaca gagttctact
     4261 gtttcgaacc atggttacca aggagaagga gaaactgggg ctggtggaaa ccagctctgc
     4321 ctccccgcat gtcactcaca tcaccatccg ccggtccagg atgctggagg acggctacga
     4381 gcagcttagg cagctctccc agcacgccat gaagggggtc atccgtgtga agtttgtcaa
     4441 tgacctcggg gtggacgaag cagggattga tcaagacggt gtttttaagg agttcttgga
     4501 agagatcatc aagagagttt ttgacccagc actcaatctg ttcaagacaa ccagtgggga
     4561 tgagaggctg tacccctcac ccacatccta catccatgag aattacctgc agctcttcga
     4621 gtttgtgggg aagatgctgg ggaaggctgt gtatgaggga attgtggtgg acgtgccatt
     4681 tgcatccttc ttcctgagcc aactgcttgg gcaccaccac agcgtcttct atagctcggt
     4741 ggatgaactg ccttctctgg actccgagtt ctataaaaac ctcacctcca tcaagcgcta
     4801 tgatggggac atcactgacc tgggcctgac gctgtcttac gacgaggacg tcatgggtca
     4861 gcttgtttgc catgaactga ttcctggagg gaagaccatt cctgttacaa atgaaaataa
     4921 aattagctac atccatctga tggcacattt tcgaatgcac actcaaataa aaaaccaaac
     4981 agctgccctc attagcggat tccgttccat tatcaaaccc gagtggatcc gaatgttctc
     5041 aactcctgaa ctgcagcgtc tcatctctgg cgacaatgct gagattgatc tggaagattt
     5101 aaagaagcac acagtctact acggtggttt ccatggaagt cacagagtca tcatctggct
     5161 ctgggatatt ctggcctccg acttcacacc ggatgagaga gctatgtttc tgaagttcgt
     5221 gaccagctgc tccagacccc cgctcctggg attcgcctac ctcaagcctc cattctccat
     5281 ccgctgcgtg gaggtgtcgg acgatcagga caccggggac actctgggca gcgtcctccg
     5341 gggcttcttc accatccgca agcgggagcc aggcggccgc ctgcccacct cctccaccgc
     5401 cttcaacctg ctcaagctgc ccaactacag caagaagagc gtcctccgcg agaagctgcg
     5461 ctacgccatc agcatgaaca cgggctttga actctccatt ctagagctag cgaattcgaa
     5521 tttaaatcgg atccgcggcc gcatggagag cgacgagagc ggcctgcccg ccatggagat
     5581 cgagtgccgc atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga
     5641 gggcaccccc aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac
     5701 cttcagcccc tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta
     5761 ccccagcggc tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac
     5821 ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga
     5881 ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt
     5941 gatcttcacc gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg
     6001 cgataacgtg ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta
     6061 cagcttcgtg gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca
     6121 gaacgggggc cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct
     6181 gggcatcgtg gagtaccagc acgccttcaa gacccccatt gccttcgcca gatcccgcgc
     6241 tcagtcgtcc aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcta
     6301 aggccgcaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
     6361 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc
     6421 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     6481 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     6541 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
     6601 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
     6661 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
     6721 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
     6781 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt
     6841 gaccggcgcc tacgctagat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     6901 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     6961 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     7021 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     7081 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     7141 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     7201 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     7261 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     7321 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     7381 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     7441 cgcaagcccg gtgcctgagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     7501 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     7561 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7621 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7681 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7741 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7801 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     7861 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7921 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7981 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     8041 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     8101 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga
     8161 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     8221 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     8281 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8341 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     8401 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     8461 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8521 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     8581 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8641 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8701 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat
     8761 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8821 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     8881 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8941 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9001 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9061 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9121 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9181 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9241 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9301 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9361 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9421 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9481 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9541 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9601 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9661 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9721 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9781 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9841 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9901 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9961 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10021 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10081 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10141 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10201 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10261 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10321 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10381 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10441 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10501 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10561 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10621 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10681 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10741 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10801 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10861 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    10921 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    10981 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    11041 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    11101 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    11161 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    11221 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    11281 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    11341 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aag
//
