LOCUS       sequence-208662-       11425 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HA tagged UBE3B in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11425)
  AUTHORS   Braganza A, Li J, Zeng X, Yates NA, Dey NB, Andrews J, Clark J, 
            Zamani L, Wang XH, St Croix C, O'Sullivan R, Garcia-Exposito L, 
            Brodsky JL, Sobol RW
  TITLE     UBE3B is a Calmodulin-Regulated, Mitochondria-Associated E3 
            Ubiquitin Ligase.
  JOURNAL   J Biol Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 
            10.1074/jbc.M116.766824.
  PUBMED    28003368
REFERENCE   2  (bases 1 to 11425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 
            10.1074/jbc.M116.766824."
COMMENT     Sequence Label: pLVX-HA-UBE3B-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..11425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    679..804
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1301..1534
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1719..1763
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2026..2141
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2199..2502
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2503..2706
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2657..2677
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2703..2727
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2822..2846
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2870..2896
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(6117..6141)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(6173..6747,6748..6750,6751..6759)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6340..6357)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6567..6586
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6779..7378
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6779..6798)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7275..7295
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7392..7980
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7445..7465)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7863..7874)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8187..8820
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8948..8964)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8948..8964)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8961..8983)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8972..8988
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8996..9002,9003..9020,9021..9026))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9041..9062
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9179..9196)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9350..9938)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9430..9449)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10109..10900,10901..10969))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10732..10751
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10970..11074)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11122..11256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11159..11178)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11213..11232
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11424..11425,1..632)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca
       61 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
      121 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
      181 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
      241 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
      301 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
      361 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
      421 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
      481 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
      541 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
      601 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
      661 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
      721 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
      781 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     1081 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     1141 gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1201 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1261 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1321 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1381 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1441 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1501 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1561 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1621 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1681 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1741 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     1801 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     1861 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     1921 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     1981 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgcctttaa aagaaaaggg
     2041 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2101 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2161 agcagagatc cagtttatcg ataagcttgg gagttccgcg ttacataact tacggtaaat
     2221 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2281 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2341 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2401 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2461 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2521 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2581 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2641 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2701 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     2761 catagaagac accgactcta ctagaggatc tatttccggt gaattcctcg agactagatc
     2821 aacaagtttg tacaaaaaag caggctccgc ggccgccccc ttcaccatgt acccatacga
     2881 tgttccagat tacgctttca ccctgtctca gacctcgaga gcatggttca tcgatagagc
     2941 ccgtcaggca cgagaagaaa ggcttgtgca gaaggaacgg gagcgggcag ctgttgtgat
     3001 ccaggcccat gtccggagtt ttctctgtcg gagtcgactg cagagagata tcaggagaga
     3061 gattgatgac ttttttaaag cagatgaccc tgagtccact aaaagaagtg cactttgtat
     3121 tttcaagatt gccaggaaac tgctgttcct attcagaatc aaagaggata atgagagatt
     3181 tgagaagttg tgtcgcagca tcctgagcag catggatgct gagaatgagc ctaaggtgtg
     3241 gtatgtgtcc ctggcttgtt ctaaggacct caccctcctt tggattcaac agatcaagaa
     3301 cattttgtgg tactgctgtg attttctcaa gcagctcaag cctgaaatcc tgcaggactc
     3361 ccgactcatc accctgtacc tcacgatgct tgtcaccttc acagacactt caacgtggaa
     3421 aattcttcgg ggaaaaggtg aaagtcttcg accagcgatg aaccacattt gtgcaaatat
     3481 aatgggacat ctcaaccagc atggatttta ttctgtgctg cagatattgt taacccgtgg
     3541 cctggcaaga ccccgtcctt gtctatccaa aggcacttta acagcagctt tttctctagc
     3601 gttacgccct gtgattgctg cacagttctc agacaatctg attcggccgt tcctcatcca
     3661 catcatgtct gtgcctgctc tggtgactca tctcagcaca gtgacccctg agcgcctcac
     3721 tgttttagaa tcccatgaca tgcttcgtaa attcatcata tttttaagag accaagatcg
     3781 atgccgtgat gtatgtgaaa gtttagaagg atgccatacg ctttgtctaa tgggcaacct
     3841 cctacacttg ggctccctca gccccagagt gttagaggag gagacagatg ggttcgtgag
     3901 tttgctcacc cagacgctgt gctactgtcg gaagtatgtg tctcagaaga agtccaacct
     3961 gacccactgg catcctgtcc ttggctggtt ctcccaatct gtggattatg gccttaacga
     4021 gtcaatgcac ttgatcacca aacagctgca gttcttgtgg ggggtgcctc tgatccggat
     4081 cttcttctgt gacatcctga gcaagaagct actggagagc caggagccag cccacgcaca
     4141 gccagcatcc cctcagaatg tgctcccagt gaagagtctc ctaaagcgtg cttttcaaaa
     4201 gtcggcatca gtccggaata ttctcaggcc tgtcgggggt aaacgggtcg actctgcaga
     4261 agtccagaag gtttgcaaca tctgtgtcct ctaccagacc tcgctgacaa ctctcacaca
     4321 gattcggctg cagatactca caggtctcac ttaccttgat gacctgcttc ccaaactgtg
     4381 ggcatttatc tgtgagctcg ggccccacgg agggttaaag ctcttcttgg aatgcctgaa
     4441 caatgacact gaagagtcca agcaactctt ggccatgctg atgctgttct gtgactgttc
     4501 gcggcacctc atcacaatcc ttgatgacat tgaagtttat gaagaacaga tttcattcaa
     4561 actggaagag ctggtcacta tctcctcttt cctgaattct tttgtgttta agatgatctg
     4621 ggatggaatt gtagagaacg ccaagggtga gaccttggag ctgttccagt ctgtccacgg
     4681 gtggcttatg gtgctgtacg agcgggactg ccggcggcgc ttcacccccg aggaccactg
     4741 gctgcgaaag gatctcaaac ctagcgtgct cttccaagaa ctcgacaggg acagaaaacg
     4801 ggcacagttg atcctgcagt acatcccaca tgtcatccct cacaaaaaca gagttctact
     4861 gtttcgaacc atggttacca aggagaagga gaaactgggg ctggtggaaa ccagctctgc
     4921 ctccccgcat gtcactcaca tcaccatccg ccggtccagg atgctggagg acggctacga
     4981 gcagcttagg cagctctccc agcacgccat gaagggggtc atccgtgtga agtttgtcaa
     5041 tgacctcggg gtggacgaag cagggattga tcaagacggt gtttttaagg agttcttgga
     5101 agagatcatc aagagagttt ttgacccagc actcaatctg ttcaagacaa ccagtgggga
     5161 tgagaggctg tacccctcac ccacatccta catccatgag aattacctgc agctcttcga
     5221 gtttgtgggg aagatgctgg ggaaggctgt gtatgaggga attgtggtgg acgtgccatt
     5281 tgcatccttc ttcctgagcc aactgcttgg gcaccaccac agcgtcttct atagctcggt
     5341 ggatgaactg ccttctctgg actccgagtt ctataaaaac ctcacctcca tcaagcgcta
     5401 tgatggggac atcactgacc tgggcctgac gctgtcttac gacgaggacg tcatgggtca
     5461 gcttgtttgc catgaactga ttcctggagg gaagaccatt cctgttacaa atgaaaataa
     5521 aattagctac atccatctga tggcacattt tcgaatgcac actcaaataa aaaaccaaac
     5581 agctgccctc attagcggat tccgttccat tatcaaaccc gagtggatcc gaatgttctc
     5641 aactcctgaa ctgcagcgtc tcatctctgg cgacaatgct gagattgatc tggaagattt
     5701 aaagaagcac acagtctact acggtggttt ccatggaagt cacagagtca tcatctggct
     5761 ctgggatatt ctggcctccg acttcacacc ggatgagaga gctatgtttc tgaagttcgt
     5821 gaccagctgc tccagacccc cgctcctggg attcgcctac ctcaagcctc cattctccat
     5881 ccgctgcgtg gaggtgtcgg acgatcagga caccggggac actctgggca gcgtcctccg
     5941 gggcttcttc accatccgca agcgggagcc aggcggccgc ctgcccacct cctccacctg
     6001 cttcaacctg ctcaagctgc ccaactacag caagaagagc gtcctccgcg agaagctgcg
     6061 ctacgccatc agcatgaaca cgggctttga actctcctag aagggtgggc gcgccgaccc
     6121 agctttcttg tacaaagtgg ttgatctagt tctagagcgg ccgcggatcc cgcccctctc
     6181 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     6241 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     6301 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     6361 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     6421 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     6481 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     6541 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     6601 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     6661 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     6721 ttttcctttg aaaaacacga tgataagctt gccacaaccc acaaggagac gaccttccat
     6781 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     6841 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     6901 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     6961 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     7021 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     7081 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     7141 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     7201 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     7261 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     7321 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctagac
     7381 gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     7441 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7501 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7561 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7621 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7681 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7741 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     7801 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7861 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7921 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7981 ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     8041 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     8101 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     8161 ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga
     8221 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     8281 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     8341 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     8401 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     8461 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     8521 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     8581 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     8641 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     8701 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     8761 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     8821 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     8881 agagtgagag gccttgacat tgctagcgtt taccgtcgac ctctagctag agcttggcgt
     8941 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     9001 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     9061 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     9121 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     9181 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     9241 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     9301 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     9361 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     9421 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     9481 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9541 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     9601 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9661 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9721 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9781 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9841 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9901 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9961 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    10021 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    10081 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    10141 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    10201 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    10261 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    10321 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    10381 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    10441 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    10501 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    10561 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    10621 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    10681 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    10741 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    10801 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    10861 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10921 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    10981 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    11041 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    11101 acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta caaataaagc
    11161 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    11221 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
    11281 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
    11341 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
    11401 atatgtacta caaacttagt agttg
//
