LOCUS       sequence-208667-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Universal mini-vector for efficient cloning of DNA and expression of
            protein in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Liu X, Li T, Hart DJ, Gao S, Wang H, Gao H, Xu S, Zhang Y, Liu Y, An
            Y
  TITLE     A universal mini-vector and an annealing of PCR products (APP)-based
            cloning strategy for convenient molecular biological manipulations.
  JOURNAL   Biochem Biophys Res Commun. 2018 Mar 18;497(4):978-982. doi: 
            10.1016/j.bbrc.2018.02.099. Epub 2018 Feb 12.
  PUBMED    29448102
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2018.02.099"; journalName: "Biochem Biophys Res 
            Commun"; date: "2018-03-18- 18"; volume: "497"; issue: "4"; pages: 
            "978-982"
COMMENT     Sequence Label: pANY1
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     643..662
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacat atgggccacc atcatcacca ccatgtttat
       61 gatattccaa ctaccgagaa tttgtatttt caggggggat ccactagtag gcctgtcgac
      121 ggtcagtccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc
      181 ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga
      241 agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga
      301 tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc
      361 cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat
      421 gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct
      481 ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc
      541 ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc
      601 gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata
      661 tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcg
//
