LOCUS       sequence-208676-       14004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of untagged human RBFOX2 in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14004)
  AUTHORS   Jangi M, Boutz PL, Paul P, Sharp PA
  TITLE     Rbfox2 controls autoregulation in RNA-binding protein networks.
  JOURNAL   Genes Dev. 2014 Mar 15;28(6):637-51. doi: 10.1101/gad.235770.113.
  PUBMED    24637117
REFERENCE   2  (bases 1 to 14004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.235770"; journalName: "Genes Dev"; date: "2014-03-15- 
            15"; volume: "28"; issue: "6"; pages: "637-51"
COMMENT     Sequence Label: pSLIK TT RBFOX2
FEATURES             Location/Qualifiers
     source          1..14004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        57..371
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    65..83
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    101..119
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    136..154
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    172..190
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    208..226
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    243..261
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    279..297
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     344..368
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(1753..1777)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1827..3038
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2875..2894
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(3163..3780,3781..3786,3787..3822,3823..3825,3826..3861,
                     3862..3864,3865..3900,3901..3909)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    3962..4535
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4129..4146)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4356..4375
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4594..5613
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             4594..5613
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    6079..6667
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6132..6152)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6550..6561)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6670..6686)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6671..6687)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7196..7376
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7402..7419)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7408..7632
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7678..8106
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7765..7784)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7975..7996
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8115..8135)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8120..8449
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8300..8435
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8362..8381
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8497..8544
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8563..8937
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9067..9188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9104..9123)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9158..9177
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9237..9253)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9237..9253)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9250..9272)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9261..9277
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9285..9291,9292..9309,9310..9315))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9330..9351
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9468..9485)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9639..10227)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9719..9738)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10398..11189,11190..11258))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11021..11040
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11259..11363)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11438..11457)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11629..12008
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12010..12208
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             12226..12406
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12453..12578
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13071..13304
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13489..13533
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13831..13948
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 acaagtttgt acaaaaaagc aggctttaaa ggaaccaatt cagtcgactg gatcccgagt
       61 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      121 cgatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca
      181 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
      241 tatccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
      301 tatgtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc
      361 gtcagatcgc cactagtcca ccatggcgga gggcgcccag ccgcagcagc cgcctcagct
      421 cgggcccggc gccgccgccc ggggcatgaa gcgggagtcg gagctggagc tgccggtgcc
      481 cggagcggga ggagacggag ccgatcccgg cctgagcaag cggccgcgca ctgaggaggc
      541 ggcggccgac ggtggcggcg ggatgcagaa cgagcctctg actccaggtt atcatggatt
      601 cccagcgcgg gacagccagg gtaaccagga gccgacaaca actcctgacg caatggttca
      661 gccttttact accatcccat ttccaccacc tccgcagaat ggaattccca cagagtatgg
      721 ggtgccacac actcaagact atgccggcca gaccggtgag cataacctga cactctacgg
      781 aagtacgcaa gcccacgggg agcagagcag caactcaccc agcacacaaa atggatctct
      841 tacgcagaca gaaggtggag cacagacaga cggccagcag tcacagacac aaagtagtga
      901 aaattcagag agtaaatcta ccccgaaacg gctgcatgtc tctaatattc ctttccgctt
      961 ccgggaccct gacctccggc agatgtttgg gcagtttggc aaaatcctag atgtagaaat
     1021 aatctttaat gaacgtggct ctaagggatt cgggttcgta actttcgaga atagtgctga
     1081 tgcagacagg gccagggaga aattacacgg caccgtggta gagggccgta aaatcgaggt
     1141 gaataatgct acagcacgtg taatgaccaa taagaagatg gtcacaccat atgcaaatgg
     1201 ttggaaatta agcccagtag ttggagctgt atatggtccg gagttatatg cagcatccag
     1261 ctttcaagca gatgtgtccc taggcaatga tgcagcagtg cccctatcag gaagaggggg
     1321 tatcaacact tacattcctt taatcagtct ccctttagtt cctggcttcc cttaccctac
     1381 tgcagccacc acggcagccg ctttcagagg agcccatttg aggggcagag ggcggacagt
     1441 atatggtgca gtccgagcgg tacctccaac agccatcccc gcctatccag gtgtggttta
     1501 ccaggacgga ttttacggtg ctgacctcta tggtggatat gcagcctaca gatatgcaca
     1561 gcctgctact gcaaccgcag ccaccgctgc tgcagccgct gcagccgctt acagtgacgg
     1621 ttatggcagg gtgtacacag ccgaccccta ccatgccctt gcccctgccg ctagctatgg
     1681 agttggcgct gtggcgagtt tataccgagg tggctacagc cgatttgccc cctactgact
     1741 cgagatatct agacccagct ttcttgtaca aagtggttga taacatctcg agccataaag
     1801 atggttaatt aacccaccca agatctggcc tccgcgccgg gttttggcgc ctcccgcggg
     1861 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga
     1921 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc
     1981 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt
     2041 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg
     2101 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct
     2161 agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca
     2221 cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt
     2281 gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg
     2341 ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg
     2401 gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg
     2461 gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat
     2521 gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt
     2581 ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct
     2641 ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag
     2701 ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt
     2761 cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat
     2821 aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc
     2881 tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac
     2941 cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt
     3001 ccgctaaatt ctggccgttt ttggcttttt tgttagacga agcttggtac cgagctcgga
     3061 tctccacccc gtaccggtcc tgcaggattt aaatggcgcg ccgctagcgc taccggactc
     3121 agatctcgag ctcaagcttc gaattctgca gtcgaattca ccatgtctag actggacaag
     3181 agcaaagtca taaacggagc tctggaatta ctcaatggtg tcggtatcga aggcctgacg
     3241 acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag
     3301 aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac
     3361 ttctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcatac
     3421 cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca
     3481 gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc
     3541 ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg
     3601 gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg
     3661 cccccacttc tgagacaagc aattgagctg ttcgaccggc agggagccga acctgccttc
     3721 cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc
     3781 gggccgaccg acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac
     3841 gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc
     3901 cccgggtaac taagtaagga tccgcggccg cactagagga attccgcccc tctccccccc
     3961 ccccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     4021 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     4081 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     4141 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     4201 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     4261 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     4321 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     4381 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     4441 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     4501 gggacgtggt tttcctttga aaaacacgat gataagctta ccggtacgcg tgatagcttg
     4561 ccacaacccg taccaaagat ggatagatcc ggaaagcctg aactcaccgc gacgtctgtc
     4621 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
     4681 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
     4741 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     4801 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
     4861 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     4921 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
     4981 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     5041 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     5101 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     5161 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     5221 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     5281 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     5341 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
     5401 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     5461 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     5521 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     5581 cgccccagca ctcgtccgag ggcaaaggaa tagagtagat gccgaccgaa caagagctga
     5641 tttcgagaac gcctcagcca gcaactcgcg cgagcctagc aaggcaaatg cgagagaacg
     5701 gccttacgct tggtggcaca gttctcgtcc acagttcgct aagctcgctc ggctgggtcg
     5761 cgggagggcc ggtcgcagtg attcaggccc ttctggattg tgttggtccc cagggcacga
     5821 ttgtcatgcc cacgcactcg ggtgatctga ctgatcccgc agattggaga tcgccgcccg
     5881 tgcctgccga ttgggtgcag atctctagat ctgtacaagt aaagcggccg cgactctaga
     5941 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     6001 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgtttagtcc ctcccaattc
     6061 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6121 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6181 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6241 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6301 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6361 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6421 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     6481 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6541 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6601 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6661 tccccgcatc gataccgtcg acctcgatcg agacctagaa aaacatggag caatcacaag
     6721 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     6781 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     6841 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     6901 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     6961 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     7021 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     7081 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     7141 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     7201 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7261 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7321 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
     7381 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     7441 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     7501 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     7561 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     7621 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     7681 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     7741 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     7801 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     7861 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     7921 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     7981 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     8041 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     8101 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     8161 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     8221 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     8281 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     8341 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     8401 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     8461 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     8521 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
     8581 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     8641 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     8701 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     8761 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     8821 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     8881 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     8941 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     9001 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     9061 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     9121 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     9181 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     9241 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     9301 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     9361 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     9421 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     9481 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9541 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9601 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9661 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9721 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9781 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9841 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9901 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9961 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    10021 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    10081 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    10141 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    10201 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10261 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    10321 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    10381 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    10441 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10501 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10561 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10621 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10681 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10741 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10801 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10861 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10921 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10981 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    11041 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    11101 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    11161 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    11221 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    11281 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    11341 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
    11401 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
    11461 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
    11521 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
    11581 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
    11641 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
    11701 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
    11761 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
    11821 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
    11881 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
    11941 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
    12001 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
    12061 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
    12121 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
    12181 gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta
    12241 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
    12301 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
    12361 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
    12421 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
    12481 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
    12541 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
    12601 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
    12661 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
    12721 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
    12781 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
    12841 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
    12901 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
    12961 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
    13021 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
    13081 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
    13141 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
    13201 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
    13261 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
    13321 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
    13381 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
    13441 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
    13501 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
    13561 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
    13621 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
    13681 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
    13741 gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt
    13801 ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
    13861 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
    13921 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
    13981 ttggttaatt ctagacagtt atca
//
