LOCUS       sequence-208678-       11425 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HA tagged UBE3B mutant C1036A in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11425)
  AUTHORS   Braganza A, Li J, Zeng X, Yates NA, Dey NB, Andrews J, Clark J, 
            Zamani L, Wang XH, St Croix C, O'Sullivan R, Garcia-Exposito L, 
            Brodsky JL, Sobol RW
  TITLE     UBE3B is a Calmodulin-Regulated, Mitochondria-Associated E3 
            Ubiquitin Ligase.
  JOURNAL   J Biol Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 
            10.1074/jbc.M116.766824.
  PUBMED    28003368
REFERENCE   2  (bases 1 to 11425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 
            10.1074/jbc.M116.766824."
COMMENT     Sequence Label: pLVX-HA-UBE3B(C1036A)-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..11425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..308
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     463..483
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     509..533
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    628..652
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             676..702
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(3923..3947)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(3979..4553,4554..4556,4557..4565)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4146..4163)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4373..4392
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4585..5184
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4585..4604)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5081..5101
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5198..5786
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5251..5271)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5669..5680)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5993..6626
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6754..6770)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6754..6770)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6767..6789)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6778..6794
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6802..6808,6809..6826,6827..6832))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6847..6868
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6985..7002)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7156..7744)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7236..7255)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7915..8706,8707..8775))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8538..8557
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8776..8880)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8928..9062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8965..8984)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9019..9038
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9230..9863
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9910..10035
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10532..10765
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10950..10994
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11257..11372
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
      541 gacgccatcc acgctgtttt gacctccata gaagacaccg actctactag aggatctatt
      601 tccggtgaat tcctcgagac tagatcaaca agtttgtaca aaaaagcagg ctccgcggcc
      661 gcccccttca ccatgtaccc atacgatgtt ccagattacg ctttcaccct gtctcagacc
      721 tcgagagcat ggttcatcga tagagcccgt caggcacgag aagaaaggct tgtgcagaag
      781 gaacgggagc gggcagctgt tgtgatccag gcccatgtcc ggagttttct ctgtcggagt
      841 cgactgcaga gagatatcag gagagagatt gatgactttt ttaaagcaga tgaccctgag
      901 tccactaaaa gaagtgcact ttgtattttc aagattgcca ggaaactgct gttcctattc
      961 agaatcaaag aggataatga gagatttgag aagttgtgtc gcagcatcct gagcagcatg
     1021 gatgctgaga atgagcctaa ggtgtggtat gtgtccctgg cttgttctaa ggacctcacc
     1081 ctcctttgga ttcaacagat caagaacatt ttgtggtact gctgtgattt tctcaagcag
     1141 ctcaagcctg aaatcctgca ggactcccga ctcatcaccc tgtacctcac gatgcttgtc
     1201 accttcacag acacttcaac gtggaaaatt cttcggggaa aaggtgaaag tcttcgacca
     1261 gcgatgaacc acatttgtgc aaatataatg ggacatctca accagcatgg attttattct
     1321 gtgctgcaga tattgttaac ccgtggcctg gcaagacccc gtccttgtct atccaaaggc
     1381 actttaacag cagctttttc tctagcgtta cgccctgtga ttgctgcaca gttctcagac
     1441 aatctgattc ggccgttcct catccacatc atgtctgtgc ctgctctggt gactcatctc
     1501 agcacagtga cccctgagcg cctcactgtt ttagaatccc atgacatgct tcgtaaattc
     1561 atcatatttt taagagacca agatcgatgc cgtgatgtat gtgaaagttt agaaggatgc
     1621 catacgcttt gtctaatggg caacctccta cacttgggct ccctcagccc cagagtgtta
     1681 gaggaggaga cagatgggtt cgtgagtttg ctcacccaga cgctgtgcta ctgtcggaag
     1741 tatgtgtctc agaagaagtc caacctgacc cactggcatc ctgtccttgg ctggttctcc
     1801 caatctgtgg attatggcct taacgagtca atgcacttga tcaccaaaca gctgcagttc
     1861 ttgtgggggg tgcctctgat ccggatcttc ttctgtgaca tcctgagcaa gaagctactg
     1921 gagagccagg agccagccca cgcacagcca gcatcccctc agaatgtgct cccagtgaag
     1981 agtctcctaa agcgtgcttt tcaaaagtcg gcatcagtcc ggaatattct caggcctgtc
     2041 gggggtaaac gggtcgactc tgcagaagtc cagaaggttt gcaacatctg tgtcctctac
     2101 cagacctcgc tgacaactct cacacagatt cggctgcaga tactcacagg tctcacttac
     2161 cttgatgacc tgcttcccaa actgtgggca tttatctgtg agctcgggcc ccacggaggg
     2221 ttaaagctct tcttggaatg cctgaacaat gacactgaag agtccaagca actcttggcc
     2281 atgctgatgc tgttctgtga ctgttcgcgg cacctcatca caatccttga tgacattgaa
     2341 gtttatgaag aacagatttc attcaaactg gaagagctgg tcactatctc ctctttcctg
     2401 aattcttttg tgtttaagat gatctgggat ggaattgtag agaacgccaa gggtgagacc
     2461 ttggagctgt tccagtctgt ccacgggtgg cttatggtgc tgtacgagcg ggactgccgg
     2521 cggcgcttca cccccgagga ccactggctg cgaaaggatc tcaaacctag cgtgctcttc
     2581 caagaactcg acagggacag aaaacgggca cagttgatcc tgcagtacat cccacatgtc
     2641 atccctcaca aaaacagagt tctactgttt cgaaccatgg ttaccaagga gaaggagaaa
     2701 ctggggctgg tggaaaccag ctctgcctcc ccgcatgtca ctcacatcac catccgccgg
     2761 tccaggatgc tggaggacgg ctacgagcag cttaggcagc tctcccagca cgccatgaag
     2821 ggggtcatcc gtgtgaagtt tgtcaatgac ctcggggtgg acgaagcagg gattgatcaa
     2881 gacggtgttt ttaaggagtt cttggaagag atcatcaaga gagtttttga cccagcactc
     2941 aatctgttca agacaaccag tggggatgag aggctgtacc cctcacccac atcctacatc
     3001 catgagaatt acctgcagct cttcgagttt gtggggaaga tgctggggaa ggctgtgtat
     3061 gagggaattg tggtggacgt gccatttgca tccttcttcc tgagccaact gcttgggcac
     3121 caccacagcg tcttctatag ctcggtggat gaactgcctt ctctggactc cgagttctat
     3181 aaaaacctca cctccatcaa gcgctatgat ggggacatca ctgacctggg cctgacgctg
     3241 tcttacgacg aggacgtcat gggtcagctt gtttgccatg aactgattcc tggagggaag
     3301 accattcctg ttacaaatga aaataaaatt agctacatcc atctgatggc acattttcga
     3361 atgcacactc aaataaaaaa ccaaacagct gccctcatta gcggattccg ttccattatc
     3421 aaacccgagt ggatccgaat gttctcaact cctgaactgc agcgtctcat ctctggcgac
     3481 aatgctgaga ttgatctgga agatttaaag aagcacacag tctactacgg tggtttccat
     3541 ggaagtcaca gagtcatcat ctggctctgg gatattctgg cctccgactt cacaccggat
     3601 gagagagcta tgtttctgaa gttcgtgacc agctgctcca gacccccgct cctgggattc
     3661 gcctacctca agcctccatt ctccatccgc tgcgtggagg tgtcggacga tcaggacacc
     3721 ggggacactc tgggcagcgt cctccggggc ttcttcacca tccgcaagcg ggagccaggc
     3781 ggccgcctgc ccacctcctc caccgccttc aacctgctca agctgcccaa ctacagcaag
     3841 aagagcgtcc tccgcgagaa gctgcgctac gccatcagca tgaacacggg ctttgaactc
     3901 tcctagaagg gtgggcgcgc cgacccagct ttcttgtaca aagtggttga tctagttcta
     3961 gagcggccgc ggatcccgcc cctctccctc ccccccccct aacgttactg gccgaagccg
     4021 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     4081 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     4141 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     4201 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     4261 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     4321 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     4381 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     4441 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     4501 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aagcttgcca
     4561 caacccacaa ggagacgacc ttccatgacc gagtacaagc ccacggtgcg cctcgccacc
     4621 cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     4681 acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     4741 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     4801 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     4861 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     4921 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     4981 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     5041 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     5101 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     5161 atgacccgca agcccggtgc ctagacgcgt ctggaacaat caacctctgg attacaaaat
     5221 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5281 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5341 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5401 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5461 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5521 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     5581 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     5641 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     5701 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     5761 gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa
     5821 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     5881 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     5941 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg
     6001 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     6061 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
     6121 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
     6181 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     6241 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
     6301 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
     6361 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
     6421 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     6481 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6541 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6601 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     6661 ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgtttacc
     6721 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6781 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6841 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6901 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6961 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7021 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7081 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7141 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7201 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7261 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7321 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7381 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7441 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7501 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7561 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7621 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7681 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7741 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7801 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7861 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7921 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7981 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8041 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8101 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8161 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8221 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8281 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8341 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8401 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8461 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8521 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8581 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8641 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8701 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8761 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8821 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8881 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg
     8941 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     9001 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     9061 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
     9121 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
     9181 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta
     9241 attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     9301 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     9361 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     9421 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
     9481 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
     9541 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
     9601 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
     9661 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     9721 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     9781 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     9841 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     9901 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     9961 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
    10021 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
    10081 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
    10141 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
    10201 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
    10261 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
    10321 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc
    10381 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
    10441 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
    10501 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
    10561 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
    10621 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
    10681 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
    10741 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
    10801 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
    10861 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
    10921 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
    10981 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
    11041 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
    11101 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
    11161 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
    11221 ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg ggggattggg
    11281 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
    11341 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
    11401 ccagtttatc gataagcttg ggagt
//
