LOCUS       sequence-208687-       13816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses pentamerised full-length protein ectodomain with no 
            N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 
            d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13816)
  AUTHORS   Knockel J, Dundas K, Yang ASP, Galaway F, Metcalf T, Gemert GV, 
            Sauerwein RW, Rayner JC, Billker O, Wright GJ
  TITLE     Systematic Identification of Plasmodium Falciparum Sporozoite 
            Membrane Protein Interactions Reveals an Essential Role for the p24 
            Complex in Host Infection.
  JOURNAL   Mol Cell Proteomics. 2021;20:100038. doi: 10.1074/mcp.RA120.002432. 
            Epub 2021 Jan 27.
  PUBMED    33515807
REFERENCE   2  (bases 1 to 13816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2021"; volume: "20:100038. doi"; pages: " 
            10.1074/mcp.RA120.002432. Epub 2021 Jan 27"
COMMENT     Sequence Label: RON2-COMP-blac-flag-his
FEATURES             Location/Qualifiers
     source          1..13816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..329
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      352..361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7348..8133
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             8140..8205
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             8212..8229
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    8267..8322
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8321..8340)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(10558..10576)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10644..10748
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10749..10817,10818..11609)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11780..12368
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12269..12288
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12522..12539
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12656..12677
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12692..12697,12698..12715,12716..12722)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12730..12746
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12735..12757
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12754..12770
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12754..12770
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        13041..13420
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        13421..13624
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     13575..13595
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     13621..13645
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgcctct
      421 gagctgagca agcacgtgaa gcacgacacc gacgagctga agtacgccag ccccacctac
      481 gaccccaaga aaggcaccaa agtgatcttc tacatgcccg gcaacgagca gggcgtgatc
      541 cccaacaaca tccagaacaa gcagcacggc accagcatct accccgccat cgagtacccc
      601 accaccaagt accctgccat tgagtatccc ggcagcgaga tgaacaacgg caagaccggc
      661 accaccaaca gcggcatcta caacaaggcc cacggcagca gcaacgacta caacgccgcc
      721 aacagccagg acaagaccat caacctgcac atcgacgaga acaacgccaa caatgcctac
      781 cagccctacg acagcaacaa tgctaacaac aacgctaact ccaacaataa cgcaaacaat
      841 aatgccaata acaacgcaaa caacaatgca aacaattatg ctaacaacaa tgccaacacg
      901 aacagcaaca ccaactccaa taccaacagt aataacaatg ccgccaatta cgacctgaac
      961 agcgagtacg agaagatccg gcggaaagag gaagaggccg ccagacggat cgagagagag
     1021 cggagagccg acatcaaccg gaagaaccag gataataacg ctaataagta caacaatgag
     1081 cagaacggcg gctacgagag cgacggcaac agccccaaca gcagagtgaa catcaatatc
     1141 gccaacaacg gcgcccacgg aaaccctcac aacaacaacg cctacggcca caagaacaac
     1201 atgcacaata ccgccaacgg caactacgcc aatggcaatt atgcaaacgg aaattacgcc
     1261 aagggcgact acaccaacgg ggattacaca aacggcgatt atacgaatgg ggactatacg
     1321 aacgggatca acaacaatat gcacgggaag aacaactaca acaccgcaaa tggcgagtac
     1381 gtgaacggcg cctatgacgg cctgaacaat ggcgcctaca agctgatcgg caacctgaac
     1441 aacaatcaga acgtggacaa cagctacaac cagggcaacg agaacgacaa gacctacacc
     1501 agcaactaca atatcaacct cgaggacaac aagaacagcc ccgataacaa ccagaactac
     1561 atcagcacct tcaacaagga catcggccct aacaaggccg agcggagcta ctacgacgtg
     1621 tacggcagag agtacgacga caacaagtac aatccgtaca ataaggccaa caaccataac
     1681 acgaaccaga atggcgccac cacctacggc tctaacgcca acggggccta tggccccaac
     1741 ggcgcttacg gcagcaatgg ggcctacgag cccaacgagg cctacggacc taatggcgca
     1801 tacggcccaa atggcgctta tggacacaac gggaagtacg gctccaaggg cacatacggc
     1861 aacaacgagg ctccctacta cgtggaacac cccgagtacg ataacggcaa gtccatgagc
     1921 gacttccacg tgaaggacag caaggacaat atcggccctg gcggcgacta ccctaacctg
     1981 taccagaaca tctacggcaa tgagaagaac cccaacatct tccccggcag ccccagaaac
     2041 atcaacgtgt acagcgtgca ccacatccct aacaacgggg ctaacggcgg cctgaatagc
     2101 ggagccaatg gcggactcaa caatggtgcc aacgatgggc ttaacaatgg ggcaaatggt
     2161 ggcctcaata acggcgcaaa cggtggactt aataatggtg ctaatggagg acttaacaac
     2221 ggaatgaata atggcatgaa caacgggatg aacaacggaa tgaacaatgg gatgaataat
     2281 ggaatgaata acggcatgaa taatggcgcc aacggcggcc ttaacaatgg gatgaacaat
     2341 ggcatgaaca atggaatgaa taatgggatg aacaatggta tccacgacga cctgtacaac
     2401 agcgagaaca gcgctttcaa caacgggctt aataacgccg gcagaaccgg actgaacaac
     2461 gcttacccac acaacggcat gctcaataat ggggccgagt acaacgtgca ctatggcaac
     2521 agcgacagca ataacgccaa tgacgccatg ctgaacgaga actactacag cgactccgac
     2581 tacgacgacc acacccctgg caacaagaaa aaggtgtaca agagcgtggc cgagagaaac
     2641 aagaagtccg cctctcagga cagcctggga gccggcttca gcgatagcga cagcgattct
     2701 gagtacgagg tggtggacgg cgagaacaaa aagtacaaga agaagaacaa agaaaacaac
     2761 gagaaggata agtatgagaa caactgggat agcaacaatt acaatagcga caacgagatc
     2821 aaggacggct acctgagcga gagcgagcgc gagtacgccc ggaacaaggc caacgagatt
     2881 gaggacaaga tgaagaaggg cgagtacagc cggaagtata agaacagcaa gagcaatgag
     2941 gccggctacg ccagcaagca gacctccgac agcgacgact ccgatatcga ggccaacgcc
     3001 ttctatgtgg acaacggcca agagatgctg atcaaagaga aagagcacta cagctccgac
     3061 tccgagcacc acaaagagga aagcgccagc atcggaaatc tgaacgtgtt ctttcccgcc
     3121 gaaaactacc acttcagcac ctacatgggc ttcgaccggc ggagcttcct gcccagcaat
     3181 gagatcgagc tggaaaagat gatcggcgcc aacttcgcca acgaagtgaa gaactactgc
     3241 tcccggcaga atgtggccca gaagatcggc gattacctga atatcgcctt cgagtactcc
     3301 agagccctgg aagaactgag atccgagatg atcctggatt tcaacaagcg gaagcacctg
     3361 acaaacaacg ccgacgacac catcctgcac atgattgaga acgccgagaa gcggaagaac
     3421 gaccctaact acaaagaagc ctacgaaaac aaggactacg caaataatgc taatatcttc
     3481 atgaatgagt acagcaaccc gctgagcacc aagtataaca agatcctgaa agagtacctc
     3541 tgccacctgt tcgtgaacaa ccccggcacc aagcccctgg aaagactgta ctacaacagc
     3601 ctggccctgg gcgagctggt ggaacccatc agaaacaagt tcaagagcct ggccagcagc
     3661 accatcgact tcaactacga gatccacatg gccagcgcct ccaacatcta cctgctggcc
     3721 cacttcctgg tgctgtccct ggcctacctg tcctacaacg agtacttcac caccggcaca
     3781 aagagcttct acagcctgcc taccatcctg accgccaact ccgacaatag cttctttatg
     3841 ctcaacgaga tgtgcaacat ccactacaac ccgaacaaga acttcaagaa ggatatcacc
     3901 ttcatcccca tcgagagccg gcccaagcgg accaccacct tctacggcga gcggcggctg
     3961 acctgcgacc tgctggaact ggtgctgaac gccatcatgc tcatcaacat caatgagatt
     4021 aacaacgttt tcagcaataa taacgtggac ggctatgaga actccctgag cttcagccac
     4081 aacgccatcc gcatcttcag caaagtgtgc cccaagatca acaatgacaa cgtcctgaag
     4141 tgcgagttcg aggaaagcag cctgtacaat cctaagatca tcaagaacga cgccagcgag
     4201 aagtccagcc agaagaacct gaagaaggcc tttgacctgc tgcggaccta cgccgagatc
     4261 gagggccaca gcgccgaggg ctccaccagc ccttactacg tgtccctgat tctggacgac
     4321 atgaagtaca acgacttcta caagtacacc ctttggtacg agccccgcga gctgatctat
     4381 ggcgacatcc ggggcatgca aatgaagaag aaaaaaaaga ccaagtacat ctacaatgac
     4441 tttatgaaga agtctaccca gctgaagaaa aagctgatca agaatgacct caagtacaac
     4501 ctgaagtcca agggcctggt gttcctgtac gccatgatcg ataagtacgg atccatcctg
     4561 aacaaagccc agaaagccaa ggtgcagttc ctgaactccg ccagctccat ccggtactac
     4621 ctgtacctca acaaagtcat cttcaagagc gccaagacat acctggacat catgaagcgg
     4681 gtgctcgaag aactgcagac cagcaccaat acccctctga agttcctcgt gcggggcaac
     4741 tacatcgaga acatcaacaa tattgcccgg aacgacaaca tgttctacgc caacctgttt
     4801 gtgctgaccg ccctgagcag acgggacccc gtgaaggatt actacaacga caaacggaag
     4861 atgctgagcg ccaccctggc cgagaagttc gccaattccg cctccatgct gatccctcac
     4921 aagctgcgga agctggtggt gtccatgaag aaaggcctgc tgaaaaagaa gctgctgacc
     4981 tctctggcca aagtgaagct gctccagcac atccccgccc acatgctgga aaacattgcc
     5041 tccagcatcc ggttcaccac ccacaccatt gccaccatgc agatcatcca gaatgccaag
     5101 tatatgtcca agcacaattt cgcccagtac gatagcaagg gcatgctggc ccggcagatc
     5161 ttcaccaagg gcggcttcgc cgagtatgcc gacaacctga tggccaagtg gttcagcaag
     5221 ggcttcgaag agtacaagag agagcagatc gagaatttca agatggaaaa cagcatcgac
     5281 agcgagctga aagactctga gcgcgaggac gagaatgacg cctccgagga atccgccaag
     5341 aagaaactgc aggatctgca gctggaagaa agggaaaaaa tgaaaaaaga gaacagcctg
     5401 ctgttcaacc aggccgacaa gtgggaccag ttcatcaaca aagaacttgt gcgcgccctg
     5461 ggcctgtggc tggaattcaa cgacaacccc gctaacgccg cctccttcgt gtacaaggtg
     5521 gtcgaggact ccaagcacct cctcgagaac aatatcgata acaatattat cttcagccgg
     5581 accgtgaagc ccaccaagca gacagccttc cggcggttct tcaacaaaat cctgagcctg
     5641 ggcaacatgc tgctgagaaa gcccagcttc agagtggaac acgccctttg gttcggcgcc
     5701 acaatcgaca tcaagaaagc cttcatcctg ctcgagaagg tgtccgagct gcacaaaatg
     5761 ctgaacaacc aggacgagag ctggctgatc aacgaagcct ttatcgagat cgtggatcac
     5821 gtggtggacc tgagcacata caaacacgtg cgcgagccct tcggcgtggc ccggaatccc
     5881 ggaatgatgg ccattaaccc taagtacgcc gagctgagcc acgagaaccg gctgcgcgag
     5941 ctgcagaaca gcatgtgcgc cgaccactgc agcagcgtgt ggaaagtgat cagctccttc
     6001 gccctgcacc acctgaaaaa ccccgacagc ctgcacacct acgagtccaa gttctccaag
     6061 aactccttcg gaaacaagat cgacgacaag gacttcgtgc acaacttcaa aatgattctg
     6121 ggcggcgacg ctgtgctgca ctacttcgac aatctgctgc ccaagaccat gaaaaaggac
     6181 ctcaaggcca tgaagtatgg cgtcagcctg accagcgcct actccctgaa gctgaccaag
     6241 atcattttca gccagatgca gctgccttac ctgagccaga tgttttacat gcaagcccct
     6301 tacttcggcc acttcatcgg caagtggcag aagaagcggc agcagagccg gctgaaagaa
     6361 atcatgagct tcatgaccct gggcagcctg tccgcctaca ccctgttcag cgccatggac
     6421 atcacccagc aggccaagga cattggcgcc ggacccgtgg ccagctgctt taccaccaga
     6481 atgagccctc cccagcagat ctgtctgaat tccgtggtca acaccgccct gtccaccagc
     6541 acccagagcg ctatgaagtg cgttttctcc gtcggcgcgc cgtcgacctc catcacggcc
     6601 tataagagtg agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc
     6661 ctgcagggag agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc
     6721 ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag
     6781 ctgtccgaaa cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt
     6841 tctggcaacc tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg
     6901 gtgatgaaag tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc
     6961 tcacccaaga tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag
     7021 gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa
     7081 ggtgaagagg tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattccggc
     7141 ggcggctccg gaggcggtac cggtggcggc gacctagccc cacagatgct tcgagaactc
     7201 caggagacta atgcggcgct gcaagacgtg agagagctct tgcgacagca ggtcaaggag
     7261 atcaccttcc tgaagaatac ggtgatggaa tgtgacgcct gcggtctaga cagaaacctg
     7321 cctccactag cacctctggg cccacaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7381 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7441 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7501 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     7561 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7621 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7681 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7741 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7801 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     7861 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     7921 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7981 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8041 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8101 gctgagatag gtgcctcact gattaagcat tggggccctg actacaaaga ccatgacggt
     8161 gattataaag atcatgacat cgattacaag gatgacgatg acaagggccc tcaccaccac
     8221 catcaccact aattaattaa ggatcccccg acctcgacct ctggctaata aaggaaattt
     8281 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     8341 agggcaaatc atttggtcga gatccctcgg agatctctag ctagaggatc gatccccgcc
     8401 ccggacgaac taaacctgac tacgacatct ctgccccttc ttcgcggggc agtgcatgta
     8461 atcccttcag ttggttggta caacttgcca actgggccct gttccacatg tgacacgggg
     8521 ggggaccaaa cacaaagggg ttctctgact gtagttgaca tccttataaa tggatgtgca
     8581 catttgccaa cactgagtgg ctttcatcct ggagcagact ttgcagtctg tggactgcaa
     8641 cacaacattg cctttatgtg taactcttgg ctgaagctct tacaccaatg ctgggggaca
     8701 tgtacctccc aggggcccag gaagactacg ggaggctaca ccaacgtcaa tcagaggggc
     8761 ctgtgtagct accgataagc ggaccctcaa gagggcatta gcaatagtgt ttataaggcc
     8821 cccttgttaa ccctaaacgg gtagcatatg cttcccgggt agtagtatat actatccaga
     8881 ctaaccctaa ttcaatagca tatgttaccc aacgggaagc atatgctatc gaattagggt
     8941 tagtaaaagg gtcctaagga acagcgatat ctcccacccc atgagctgtc acggttttat
     9001 ttacatgggg tcaggattcc acgagggtag tgaaccattt tagtcacaag ggcagtggct
     9061 gaagatcaag gagcgggcag tgaactctcc tgaatcttcg cctgcttctt cattctcctt
     9121 cgtttagcta atagaataac tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga
     9181 aaacaaggtt tcaggtgacg cccccagaat aaaatttgga cggggggttc agtggtggca
     9241 ttgtgctatg acaccaatat aaccctcaca aaccccttgg gcaataaata ctagtgtagg
     9301 aatgaaacat tctgaatatc tttaacaata gaaatccatg gggtggggac aagccgtaaa
     9361 gactggatgt ccatctcaca cgaatttatg gctatgggca acacataatc ctagtgcaat
     9421 atgatactgg ggttattaag atgtgtccca ggcagggacc aagacaggtg aaccatgttg
     9481 ttacactcta tttgtaacaa ggggaaagag agtggacgcc gacagcagcg gactccactg
     9541 gttgtctcta acacccccga aaattaaacg gggctccacg ccaatggggc ccataaacaa
     9601 agacaagtgg ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca
     9661 cgccgccctg cggttttgga ctgtaaaata agggtgtaat aacttggctg attgtaaccc
     9721 cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac taatggcacc ccggggaata
     9781 cctgcataag taggtgggcg ggccaagata ggggcgcgat tgctgcgatc tggaggacaa
     9841 attacacaca cttgcgcctg agcgccaagc acagggttgt tggtcctcat attcacgagg
     9901 tcgctgagag cacggtgggc taatgttgcc atgggtagca tatactaccc aaatatctgg
     9961 atagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg
    10021 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg
    10081 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
    10141 gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctaat agagattagg
    10201 gtagtatatg ctatcctaat ttatatctgg gtagcatata ctacccaaat atctggatag
    10261 catatgctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
    10321 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
    10381 tatatgctat cctaatttat atctgggtag cataggctat cctaatctat atctgggtag
    10441 catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag
    10501 catatgctat cctcatgata agctgtcaaa catgagaatt ttcttgaaga cgaaagggcc
    10561 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
    10621 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    10681 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    10741 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    10801 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    10861 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    10921 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    10981 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    11041 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    11101 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    11161 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    11221 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    11281 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    11341 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    11401 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    11461 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    11521 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    11581 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    11641 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    11701 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    11761 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    11821 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    11881 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    11941 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    12001 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    12061 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    12121 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    12181 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    12241 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    12301 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    12361 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    12421 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    12481 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    12541 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    12601 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    12661 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    12721 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    12781 ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat cgcagatccg
    12841 ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga tctatacatt
    12901 gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg
    12961 ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg
    13021 tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac
    13081 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
    13141 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
    13201 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
    13261 tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa
    13321 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac
    13381 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
    13441 caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
    13501 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa
    13561 ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
    13621 agctcgttta gtgaaccgtc agatcctcac tctcttccgc atcgctgtct gcgagggcca
    13681 gctgttgggc tcgcggttga ggacaaactc ttcgcggtct ttccagtact cttggatcgg
    13741 aaacccgtcg gcctccgaac ggtactccgc caccgaggga cctgagcgag tccgcatcga
    13801 ccggatcgga aaacct
//
