LOCUS       sequence-208692-       11425 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HA tagged UBE3B in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11425)
  AUTHORS   Braganza A, Li J, Zeng X, Yates NA, Dey NB, Andrews J, Clark J, 
            Zamani L, Wang XH, St Croix C, O'Sullivan R, Garcia-Exposito L, 
            Brodsky JL, Sobol RW
  TITLE     UBE3B is a Calmodulin-Regulated, Mitochondria-Associated E3 
            Ubiquitin Ligase.
  JOURNAL   J Biol Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 
            10.1074/jbc.M116.766824.
  PUBMED    28003368
REFERENCE   2  (bases 1 to 11425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 
            10.1074/jbc.M116.766824."
COMMENT     Sequence Label: pLVX-UBE3B-HA-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..11425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2200..2503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2504..2707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2658..2678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2704..2728
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2823..2847
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6072..6098
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(6118..6142)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(6174..6748,6749..6751,6752..6760)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6341..6358)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6568..6587
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6780..7379
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6780..6799)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7276..7296
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7393..7981
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7446..7466)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7864..7875)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8188..8821
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8949..8965)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8949..8965)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8962..8984)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8973..8989
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8997..9003,9004..9021,9022..9027))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9042..9063
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9180..9197)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9351..9939)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9431..9450)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10110..10901,10902..10970))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10733..10752
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10971..11075)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11123..11257
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11160..11179)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11214..11233
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11425,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gataagcttg ggagttccgc gttacataac ttacggtaaa
     2221 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2281 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2341 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2401 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2461 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     2521 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2581 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2641 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2701 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2761 ccatagaaga caccgactct actagaggat ctatttccgg tgaattcctc gagactagat
     2821 caacaagttt gtacaaaaaa gcaggctccg cggccgcccc cttcaccatg ttcaccctgt
     2881 ctcagacctc gagagcatgg ttcatcgata gagcccgtca ggcacgagaa gaaaggcttg
     2941 tgcagaagga acgggagcgg gcagctgttg tgatccaggc ccatgtccgg agttttctct
     3001 gtcggagtcg actgcagaga gatatcagga gagagattga tgactttttt aaagcagatg
     3061 accctgagtc cactaaaaga agtgcacttt gtattttcaa gattgccagg aaactgctgt
     3121 tcctattcag aatcaaagag gataatgaga gatttgagaa gttgtgtcgc agcatcctga
     3181 gcagcatgga tgctgagaat gagcctaagg tgtggtatgt gtccctggct tgttctaagg
     3241 acctcaccct cctttggatt caacagatca agaacatttt gtggtactgc tgtgattttc
     3301 tcaagcagct caagcctgaa atcctgcagg actcccgact catcaccctg tacctcacga
     3361 tgcttgtcac cttcacagac acttcaacgt ggaaaattct tcggggaaaa ggtgaaagtc
     3421 ttcgaccagc gatgaaccac atttgtgcaa atataatggg acatctcaac cagcatggat
     3481 tttattctgt gctgcagata ttgttaaccc gtggcctggc aagaccccgt ccttgtctat
     3541 ccaaaggcac tttaacagca gctttttctc tagcgttacg ccctgtgatt gctgcacagt
     3601 tctcagacaa tctgattcgg ccgttcctca tccacatcat gtctgtgcct gctctggtga
     3661 ctcatctcag cacagtgacc cctgagcgcc tcactgtttt agaatcccat gacatgcttc
     3721 gtaaattcat catattttta agagaccaag atcgatgccg tgatgtatgt gaaagtttag
     3781 aaggatgcca tacgctttgt ctaatgggca acctcctaca cttgggctcc ctcagcccca
     3841 gagtgttaga ggaggagaca gatgggttcg tgagtttgct cacccagacg ctgtgctact
     3901 gtcggaagta tgtgtctcag aagaagtcca acctgaccca ctggcatcct gtccttggct
     3961 ggttctccca atctgtggat tatggcctta acgagtcaat gcacttgatc accaaacagc
     4021 tgcagttctt gtggggggtg cctctgatcc ggatcttctt ctgtgacatc ctgagcaaga
     4081 agctactgga gagccaggag ccagcccacg cacagccagc atcccctcag aatgtgctcc
     4141 cagtgaagag tctcctaaag cgtgcttttc aaaagtcggc atcagtccgg aatattctca
     4201 ggcctgtcgg gggtaaacgg gtcgactctg cagaagtccg gaaggtttgc aacatctgtg
     4261 tcctctacca gacctcgctg acaactctca cacagattcg gctgcagata ctcacaggtc
     4321 tcacttacct tgatgacctg cttcccaaac tgtgggcatt tatctgtgag ctcgggcccc
     4381 acggagggtt aaagctcttc ttggaatgcc tgaacaatga cactgaagag tccaagcaac
     4441 tcttggccat gctgatgctg ttctgtgact gttcgcggca cctcatcaca atccttgatg
     4501 acattgaagt ttatgaagaa cagatttcat tcaaactgga agagctggtc actatctcct
     4561 ctttcctgaa ttcttttgtg tttaagatga tctgggatgg aattgtagag aacgccaagg
     4621 gtgagacctt ggagctgttc cagtctgtcc acgggtggct tatggtgctg tacgagcggg
     4681 actgccggcg gcgcttcacc cccgaggacc actggctgcg aaaggatctc aaacctagcg
     4741 tgctcttcca agaactcgac agggacagaa aacgggcaca gttgatcctg cagtacatcc
     4801 cacatgtcat ccctcacaaa aacagagttc tactgtttcg aaccatggtt accaaggaga
     4861 aggagaaact ggggctggtg gaaaccagct ctgcctcccc gcatgtcact cacatcacca
     4921 tccgccggtc caggatgctg gaggacggct acgagcagct taggcagctc tcccagcacg
     4981 ccatgaaggg ggtcatccgt gtgaagtttg tcaatgacct cggggtggac gaagcaggga
     5041 ttgatcaaga cggtgttttt aaggagttct tggaagagat catcaagaga gtttttgacc
     5101 cagcactcaa tctgttcaag acaaccagtg gggatgagag gctgtacccc tcacccacat
     5161 cctacatcca tgagaattac ctgcagctct tcgagtttgt ggggaagatg ctggggaagg
     5221 ctgtgtatga gggaattgtg gtggacgtgc catttgcatc cttcttcctg agccaactgc
     5281 ttgggcacca ccacagcgtc ttctatagct cggtggatga actgccttct ctggactccg
     5341 agttctataa aaacctcacc tccatcaagc gctatgatgg ggacatcact gacctgggcc
     5401 tgacgctgtc ttacgacgag gacgtcatgg gtcagcttgt ttgccatgaa ctgattcctg
     5461 gagggaagac cattcctgtt acaaatgaaa ataaaattag ctacatccat ctgatggcac
     5521 attttcgaat gcacactcaa ataaaaaacc aaacagctgc cctcattagc ggattccgtt
     5581 ccattatcaa acccgagtgg atccgaatgt tctcaactcc tgaactgcag cgtctcatct
     5641 ctggcgacaa tgctgagatt gatctggaag atttaaagaa gcacacagtc tactacggtg
     5701 gtttccatgg aagtcacaga gtcatcatct ggctctggga tattctggcc tccgacttca
     5761 caccggatga gagagctatg tttctgaagt tcgtgaccag ctgctccaga cccccgctcc
     5821 tgggattcgc ctacctcaag cctccattct ccatccgctg cgtggaggtg tcggacgatc
     5881 aggacaccgg ggacactctg ggcagcgtcc tccggggctt cttcaccatc cgcaagcggg
     5941 agccaggcgg ccgcctgccc acctcctcca cctgcttcaa cctgctcaag ctgcccaact
     6001 acagcaagaa gagcgtcctc cgcgagaagc tgcgctacgc catcagcatg aacacgggct
     6061 ttgaactctc ctacccatac gatgttccag attacgctta gaagggtggg cgcgccgacc
     6121 cagctttctt gtacaaagtg gttgatctag ttctagagcg gccgcggatc ccgcccctct
     6181 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     6241 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     6301 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     6361 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     6421 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     6481 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     6541 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     6601 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     6661 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     6721 gttttccttt gaaaaacacg atgataagct tgccacaacc cacaaggaga cgaccttcca
     6781 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac
     6841 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc
     6901 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     6961 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     7021 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     7081 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     7141 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     7201 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     7261 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     7321 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctaga
     7381 cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     7441 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     7501 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     7561 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     7621 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7681 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7741 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     7801 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7861 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7921 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7981 cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata
     8041 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     8101 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     8161 gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag
     8221 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     8281 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     8341 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     8401 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     8461 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
     8521 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
     8581 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     8641 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     8701 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8761 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     8821 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     8881 gagagtgaga ggccttgaca ttgctagcgt ttaccgtcga cctctagcta gagcttggcg
     8941 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     9001 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     9061 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     9121 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9181 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9241 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9301 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9361 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9421 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9481 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9541 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9601 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     9661 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9721 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9781 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9841 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9901 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9961 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    10021 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    10081 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    10141 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10201 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10261 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10321 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10381 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10441 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10501 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10561 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10621 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10681 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10741 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10801 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10861 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10921 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10981 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    11041 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    11101 gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag
    11161 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    11221 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    11281 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    11341 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    11401 aatatgtact acaaacttag tagtt
//
