LOCUS       sequence-208701-       14064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of FLAG-HA-tagged human RBFOX2 in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14064)
  AUTHORS   Jangi M, Boutz PL, Paul P, Sharp PA
  TITLE     Rbfox2 controls autoregulation in RNA-binding protein networks.
  JOURNAL   Genes Dev. 2014 Mar 15;28(6):637-51. doi: 10.1101/gad.235770.113.
  PUBMED    24637117
REFERENCE   2  (bases 1 to 14064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.235770"; journalName: "Genes Dev"; date: "2014-03-15- 
            15"; volume: "28"; issue: "6"; pages: "637-51"
COMMENT     Sequence Label: pSLIK TT FLAG-HA RBFOX2
FEATURES             Location/Qualifiers
     source          1..14064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        57..371
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    65..83
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    101..119
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    136..154
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    172..190
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    208..226
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    243..261
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    279..297
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     344..368
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             386..409
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             410..436
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(1813..1837)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1887..3098
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2935..2954
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(3223..3840,3841..3846,3847..3882,3883..3885,3886..3921,
                     3922..3924,3925..3960,3961..3969)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4022..4595
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4189..4206)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4416..4435
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4654..5673
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             4654..5673
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    6139..6727
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6192..6212)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6610..6621)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6730..6746)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6731..6747)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7256..7436
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7462..7479)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7468..7692
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7738..8166
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7825..7844)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8035..8056
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8175..8195)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8180..8509
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8360..8495
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8422..8441
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8557..8604
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8623..8997
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9127..9248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9164..9183)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9218..9237
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9297..9313)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9297..9313)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9310..9332)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9321..9337
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9345..9351,9352..9369,9370..9375))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9390..9411
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9528..9545)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9699..10287)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9779..9798)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10458..11249,11250..11318))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11081..11100
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11319..11423)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11498..11517)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11689..12068
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12070..12268
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             12286..12466
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12513..12638
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13131..13364
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13549..13593
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13891..14008
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 acaagtttgt acaaaaaagc aggctttaaa ggaaccaatt cagtcgactg gatcccgagt
       61 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      121 cgatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca
      181 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
      241 tatccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
      301 tatgtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc
      361 gtcagatcgc cactagtcca ccatggacta caaggacgac gatgacaagt acccttatga
      421 cgtgcccgat tacgctttaa ttaaagcgga gggcgcccag ccgcagcagc cgcctcagct
      481 cgggcccggc gccgccgccc ggggcatgaa gcgggagtcg gagctggagc tgccggtgcc
      541 cggagcggga ggagacggag ccgatcccgg cctgagcaag cggccgcgca ctgaggaggc
      601 ggcggccgac ggtggcggcg ggatgcagaa cgagcctctg actccaggtt atcatggatt
      661 cccagcgcgg gacagccagg gtaaccagga gccgacaaca actcctgacg caatggttca
      721 gccttttact accatcccat ttccaccacc tccgcagaat ggaattccca cagagtatgg
      781 ggtgccacac actcaagact atgccggcca gaccggtgag cataacctga cactctacgg
      841 aagtacgcaa gcccacgggg agcagagcag caactcaccc agcacacaaa atggatctct
      901 tacgcagaca gaaggtggag cacagacaga cggccagcag tcacagacac aaagtagtga
      961 aaattcagag agtaaatcta ccccgaaacg gctgcatgtc tctaatattc ctttccgctt
     1021 ccgggaccct gacctccggc agatgtttgg gcagtttggc aaaatcctag atgtagaaat
     1081 aatctttaat gaacgtggct ctaagggatt cgggttcgta actttcgaga atagtgctga
     1141 tgcagacagg gccagggaga aattacacgg caccgtggta gagggccgta aaatcgaggt
     1201 gaataatgct acagcacgtg taatgaccaa taagaagatg gtcacaccat atgcaaatgg
     1261 ttggaaatta agcccagtag ttggagctgt atatggtccg gagttatatg cagcatccag
     1321 ctttcaagca gatgtgtccc taggcaatga tgcagcagtg cccctatcag gaagaggggg
     1381 tatcaacact tacattcctt taatcagtct ccctttagtt cctggcttcc cttaccctac
     1441 tgcagccacc acggcagccg ctttcagagg agcccatttg aggggcagag ggcggacagt
     1501 atatggtgca gtccgagcgg tacctccaac agccatcccc gcctatccag gtgtggttta
     1561 ccaggacgga ttttacggtg ctgacctcta tggtggatat gcagcctaca gatatgcaca
     1621 gcctgctact gcaaccgcag ccaccgctgc tgcagccgct gcagccgctt acagtgacgg
     1681 ttatggcagg gtgtacacag ccgaccccta ccatgccctt gcccctgccg ctagctatgg
     1741 agttggcgct gtggcgagtt tataccgagg tggctacagc cgatttgccc cctactgact
     1801 cgagatatct agacccagct ttcttgtaca aagtggttga taacatctcg agccataaag
     1861 atggttaatt aacccaccca agatctggcc tccgcgccgg gttttggcgc ctcccgcggg
     1921 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga
     1981 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc
     2041 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt
     2101 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg
     2161 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct
     2221 agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca
     2281 cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt
     2341 gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg
     2401 ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg
     2461 gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg
     2521 gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat
     2581 gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt
     2641 ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct
     2701 ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag
     2761 ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt
     2821 cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat
     2881 aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc
     2941 tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac
     3001 cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt
     3061 ccgctaaatt ctggccgttt ttggcttttt tgttagacga agcttggtac cgagctcgga
     3121 tctccacccc gtaccggtcc tgcaggattt aaatggcgcg ccgctagcgc taccggactc
     3181 agatctcgag ctcaagcttc gaattctgca gtcgaattca ccatgtctag actggacaag
     3241 agcaaagtca taaacggagc tctggaatta ctcaatggtg tcggtatcga aggcctgacg
     3301 acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag
     3361 aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac
     3421 ttctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcatac
     3481 cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca
     3541 gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc
     3601 ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg
     3661 gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg
     3721 cccccacttc tgagacaagc aattgagctg ttcgaccggc agggagccga acctgccttc
     3781 cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc
     3841 gggccgaccg acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac
     3901 gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc
     3961 cccgggtaac taagtaagga tccgcggccg cactagagga attccgcccc tctccccccc
     4021 ccccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     4081 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     4141 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     4201 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     4261 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     4321 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     4381 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     4441 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     4501 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     4561 gggacgtggt tttcctttga aaaacacgat gataagctta ccggtacgcg tgatagcttg
     4621 ccacaacccg taccaaagat ggatagatcc ggaaagcctg aactcaccgc gacgtctgtc
     4681 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
     4741 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
     4801 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     4861 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
     4921 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     4981 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
     5041 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     5101 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     5161 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     5221 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     5281 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     5341 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     5401 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
     5461 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     5521 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     5581 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     5641 cgccccagca ctcgtccgag ggcaaaggaa tagagtagat gccgaccgaa caagagctga
     5701 tttcgagaac gcctcagcca gcaactcgcg cgagcctagc aaggcaaatg cgagagaacg
     5761 gccttacgct tggtggcaca gttctcgtcc acagttcgct aagctcgctc ggctgggtcg
     5821 cgggagggcc ggtcgcagtg attcaggccc ttctggattg tgttggtccc cagggcacga
     5881 ttgtcatgcc cacgcactcg ggtgatctga ctgatcccgc agattggaga tcgccgcccg
     5941 tgcctgccga ttgggtgcag atctctagat ctgtacaagt aaagcggccg cgactctaga
     6001 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     6061 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgtttagtcc ctcccaattc
     6121 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6181 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6241 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6301 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6361 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6421 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6481 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     6541 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6601 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6661 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6721 tccccgcatc gataccgtcg acctcgatcg agacctagaa aaacatggag caatcacaag
     6781 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     6841 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     6901 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     6961 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     7021 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     7081 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     7141 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     7201 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     7261 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7321 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7381 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
     7441 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     7501 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     7561 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     7621 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     7681 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     7741 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     7801 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     7861 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     7921 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     7981 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     8041 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     8101 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     8161 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     8221 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     8281 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     8341 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     8401 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     8461 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     8521 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     8581 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
     8641 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     8701 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     8761 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     8821 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     8881 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     8941 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     9001 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     9061 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     9121 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     9181 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     9241 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     9301 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     9361 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     9421 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     9481 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     9541 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9601 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9661 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9721 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9781 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9841 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9901 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9961 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    10021 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    10081 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    10141 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    10201 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    10261 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10321 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    10381 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    10441 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    10501 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10561 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10621 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10681 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10741 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10801 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10861 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10921 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10981 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    11041 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    11101 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    11161 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    11221 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    11281 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    11341 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    11401 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
    11461 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
    11521 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
    11581 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
    11641 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
    11701 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
    11761 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
    11821 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
    11881 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
    11941 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
    12001 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
    12061 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
    12121 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
    12181 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
    12241 gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta
    12301 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
    12361 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
    12421 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
    12481 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
    12541 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
    12601 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
    12661 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
    12721 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
    12781 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
    12841 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
    12901 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
    12961 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
    13021 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
    13081 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
    13141 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
    13201 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
    13261 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
    13321 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
    13381 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
    13441 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
    13501 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
    13561 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
    13621 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
    13681 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
    13741 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
    13801 gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt
    13861 ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
    13921 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
    13981 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
    14041 ttggttaatt ctagacagtt atca
//
