LOCUS       sequence-208718-       11257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses pentamerised full-length protein ectodomain with no 
            N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 
            d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11257)
  AUTHORS   Knockel J, Dundas K, Yang ASP, Galaway F, Metcalf T, Gemert GV, 
            Sauerwein RW, Rayner JC, Billker O, Wright GJ
  TITLE     Systematic Identification of Plasmodium Falciparum Sporozoite 
            Membrane Protein Interactions Reveals an Essential Role for the p24 
            Complex in Host Infection.
  JOURNAL   Mol Cell Proteomics. 2021;20:100038. doi: 10.1074/mcp.RA120.002432. 
            Epub 2021 Jan 27.
  PUBMED    33515807
REFERENCE   2  (bases 1 to 11257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2021"; volume: "20:100038. doi"; pages: " 
            10.1074/mcp.RA120.002432. Epub 2021 Jan 27"
COMMENT     Sequence Label: PF3D7_1123500-COMP-blac-flag-his
FEATURES             Location/Qualifiers
     source          1..11257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..329
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      352..361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3231..3242)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             4789..5574
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             5581..5646
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5653..5670
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5708..5763
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5762..5781)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7999..8017)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8085..8189
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8190..8258,8259..9050)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9221..9809
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9710..9729
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9963..9980
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10097..10118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10133..10138,10139..10156,10157..10163)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10171..10187
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10176..10198
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10195..10211
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10195..10211
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        10482..10861
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10862..11065
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11016..11036
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11062..11086
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccaac
      421 aacaacaatg ctaacaacaa taacaacaac ttcgccaaca tcaataataa caatgccaat
      481 aacggcatcc acaacgacaa caccaagaac cggcctgccg ccggaagcct gaagaacatg
      541 gaagatctgc tgagctactt caaagagcac acgaacaaca acaacaacaa tgacaaccac
      601 gtcgagctgt tcaagaactt cctgaacacc aacaaagaga gcagcggcag caatatcaat
      661 accctgatga agttcctgaa ctccgccacc aagaatgaca acaccggcaa caaccacctg
      721 gacatcggca agctgctgga caccctgaaa gagttcaaca acgcaaacaa gaacaataac
      781 gtgcccagcg ggaacaaccc caacaaccag gccaccaacc ccgctaacaa caacaacaat
      841 aacaacaacc tgaacaagga caactacgtg cagatctttg gcgacctgct caagaacatg
      901 aacatcccga gcaataacaa caacaataac aataataaca acaacaacaa caataacaat
      961 aacaacaaca ataataacaa tgccgccaac aacaacgccg cctccaacaa caacaacgct
     1021 gctagtaaca acaacaacgc agccaacaat aacaacaaca acgccaaggc cgccaacccc
     1081 atggaacagc tgaccaacct gttcagccac ataaataaca acgaccacca cgacgagaag
     1141 ggcgacggcc accctctgga acacatcatg aatctgttcg gccagggcca caacaacggc
     1201 gacgccaagg gcggcaacaa tcccctcgag cacctgacca gcctgctggg acagggacac
     1261 aatgacgggg atggcaaagg cggaaacaac ccactggaac atctgaagaa gatctttgac
     1321 caggggcaca acgatgggga cggaaagggc ggctctaacc ctcttgagca tctgacctcc
     1381 ctgctgtctc agggccataa tgatggcgac gggaaaggtg gtaacaaccc tctgcagcac
     1441 ctcacatcac tgctgagcca gggacataac aatggtgatg ggaagggcgg atccaatccg
     1501 ctggaacacc tgacttctct gctgtcccaa ggacataacg acggagacgg caagggtgga
     1561 aataaccccg ccgagtacct gaagaagctg ttcggcaacc tgcagggcaa caatgcaaac
     1621 ggcatcaacg gcaagaatat gggcgatctg cagagcatgc tgaacaacct gctgaacagc
     1681 gagaagtaca agaagtcccc ttacaccctg gacaagaccg tgtcctccgg cttcggcagc
     1741 cggtccaaag aatccatgct gctggaatac cagcacaaca aggccgagca agagatcctg
     1801 agagagctgc aagagctgat caacaaaaac aagatcaaga atgtggccga gaacaacgag
     1861 aagctgaagc agtacttctc catgctgaag ggcaagtaca acccctacag ctacgagcgg
     1921 aagatcctga acgacatcat caacgaggac gacatcaaga cgaagtacaa catcgacgac
     1981 aacttcgaga agaacctgat cgtgaacgtg cagaacgaca atatggaata cgacgtcgag
     2041 atgaacaacg aagagaaaga aaagaaaacc gagatgttcg aggacatcaa tgagctggac
     2101 gacaaggacg ccctgtccag cggctccatc aacagcgccc tgatgcggaa gaacaaggcc
     2161 aagaacgaga aactgctgcg gggcctgacc accaatatca acgacgacaa tgataacctg
     2221 cacgagtgca actgcaacaa caaactcaac aactgcgtgc tgtcctacat caactacaac
     2281 aatctcgagt acatgctgca gtccctcgac atcgacgtga agggcctgat ccggaagcac
     2341 atgctgctca aaaagaacaa caacaacaac aataataaca acaataacaa tggcaacaac
     2401 ctgtacttcg agaaaaacga ggccttcctg ctgcccctgc ctcccctgac cacaaagtcc
     2461 aatgtgcaga acaccgccaa ttacgtgctg cgggtgcccc gggtgctgtt cctggccagc
     2521 agaaagagct tcagcagcag cttcgacaga agcctgttcc acatctacaa catcatggac
     2581 acccagctga agtggaatac ctacctgtgg ggctacggct tcaagtacta ccccatcttc
     2641 taccccaaga acctgcacac cctgctgcac agccacgaga agcagatcgg cagcgagccc
     2701 ttcgacctga tcttcgtgca cagctccttc gtgtccaact actacaatta ctacttcttc
     2761 ctgaaaaaca tgcccaagat caccaccctc atcttcatga acgacggctg ggacaacaac
     2821 gtgaagaata tcttcctcaa cctgtttccc cacgtgttct tccagaatca agtgaatatc
     2881 tttgagttca atcccctgaa caatatcgac accgtcgccg agaagaagaa actgttccac
     2941 tccctgtgga acaaagtgac ctccggcacc accgccccta gcagctccat gcacgaccac
     3001 gtgatcacca acaagcacaa gaagcgcaag aagatcgaga caaagaccaa caacgtcgtg
     3061 aacaatgagg acgacgtgct gctgaccggc gaccacagca gctcctctag cagcagccct
     3121 ccctcaaaca ataacaacaa caacaataac aacatcaaca aatacgacga agaggacgat
     3181 gaagagaaca aagccctgtg ggccttcatc cctcacggcg tgaaccactg ctgcagctcc
     3241 cactactata agtcctttat catgaatgac gccagcgaca acaacaataa caacaacaac
     3301 tacaacggcg agggcgagat gctgcaggac gataagcaga atcggaacgt ccagaaggtg
     3361 gtcaacccca atttctacta cgaccgggcc agatccctgg aaaatcagca cacccctgcc
     3421 tacgagctga gctattccct gctggacctg ttcctgcacg aatgtagctt caatgagaag
     3481 gacatcttta acaacgctaa gcgggacatc gacatcctgt acctgtacaa cacggccaac
     3541 aattataacg actacctggt gcagaaaatc atggactaca tctataataa taatatgaac
     3601 aatctgaagg acaagatcca gaaatacgag gtggacctga actactggaa gctgtggggc
     3661 ctgcagacca cccacaagct gattaagaac aagatgatcg actacaccaa catgctccag
     3721 cggagcaaag tgtgcatcat cagcagcaag ttcgccggca tgctcaacaa gatggtcatc
     3781 gatgccctgt tctacggctg cgtggtcatc agcgacaaga cccacaacaa agatatcaac
     3841 aagttcatcg tcaccaccaa ggtgcccttc gagtactacg agatcagcga cctcatctat
     3901 aacaaagaaa acatggcctc cctggccaac gatctgatcg acaagattaa caccgccctc
     3961 gaggaagtga agtccggcaa gcggaaccag atgcggatcg acgtgttcaa gaccggcgcg
     4021 ccgtcgacct ccatcacggc ctataagagt gagggggagt cagcggagtt ctccttccca
     4081 ctcaaccttg gagaggaaag cctgcaggga gagttgagat ggaaggcaga gaaggctcct
     4141 tcttcccagt cctggatcac cttctcccta aagaaccaaa aggtgtctgt gcagaagtct
     4201 actagcaacc ccaagttcca gctgtccgaa acgctcccac tcacccttca gataccccag
     4261 gtctcccttc agtttgctgg ttctggcaac ctgaccctga ctctggacag agggatactg
     4321 tatcaggaag tgaacctggt ggtgatgaaa gtgactcagc ccgacagcaa cactttgacc
     4381 tgtgaggtga tgggacccac ctcacccaag atgagactga tcttgaagca ggagaatcag
     4441 gaggccaggg tctccaggca ggagaaagtg attcaagtgc aggcccctga agcaggggtg
     4501 tggcaatgtc tactgagtga aggtgaagag gtcaagatgg actccaagat ccaggtttta
     4561 tccaaagggt tgaattccgg cggcggctcc ggaggcggta ccggtggcgg cgacctagcc
     4621 ccacagatgc ttcgagaact ccaggagact aatgcggcgc tgcaagacgt gagagagctc
     4681 ttgcgacagc aggtcaagga gatcaccttc ctgaagaata cggtgatgga atgtgacgcc
     4741 tgcggtctag acagaaacct gcctccacta gcacctctgg gcccacaccc agaaacgctg
     4801 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4861 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     4921 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     4981 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5041 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5101 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5161 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5221 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5281 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5341 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5401 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5461 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5521 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggggccct
     5581 gactacaaag accatgacgg tgattataaa gatcatgaca tcgattacaa ggatgacgat
     5641 gacaagggcc ctcaccacca ccatcaccac taattaatta aggatccccc gacctcgacc
     5701 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
     5761 tcactcggaa ggacatatgg gagggcaaat catttggtcg agatccctcg gagatctcta
     5821 gctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc tctgcccctt
     5881 cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc aactgggccc
     5941 tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac tgtagttgac
     6001 atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc tggagcagac
     6061 tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg gctgaagctc
     6121 ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac gggaggctac
     6181 accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca agagggcatt
     6241 agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat gcttcccggg
     6301 tagtagtata tactatccag actaacccta attcaatagc atatgttacc caacgggaag
     6361 catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata tctcccaccc
     6421 catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta gtgaaccatt
     6481 ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc
     6541 gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt gtgaacagta
     6601 aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg
     6661 acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac aaaccccttg
     6721 ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat agaaatccat
     6781 ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat ggctatgggc
     6841 aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc aggcagggac
     6901 caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga gagtggacgc
     6961 cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac ggggctccac
     7021 gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat tgtggagtgg
     7081 gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat aagggtgtaa
     7141 taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc cacaaaacca
     7201 ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat aggggcgcga
     7261 ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag cacagggttg
     7321 ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc catgggtagc
     7381 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatag
     7441 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     7501 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     7561 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat
     7621 gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg ggtagcatat
     7681 actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta gcatatgcta
     7741 tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     7801 tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta
     7861 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     7921 tcctaatctg tatccgggta gcatatgcta tcctcatgat aagctgtcaa acatgagaat
     7981 tttcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     8041 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     8101 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     8161 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     8221 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     8281 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     8341 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     8401 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     8461 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     8521 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     8581 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     8641 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     8701 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac
     8761 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8821 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8881 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8941 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     9001 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     9061 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     9121 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     9181 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     9241 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     9301 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     9361 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     9421 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     9481 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     9541 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     9601 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9661 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9721 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9781 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9841 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9901 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     9961 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
    10021 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    10081 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    10141 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    10201 acagctatga ccatgattac gccaagctct agctagaggt cgaccaattc tcatgtttga
    10261 cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc agaactggta
    10321 ggtatggaag atctatacat tgaatcaata ttggcaatta gccatattag tcattggtta
    10381 tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat
    10441 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag
    10501 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
    10561 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
    10621 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
    10681 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
    10741 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
    10801 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
    10861 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt
    10921 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
    10981 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg
    11041 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcctca ctctcttccg
    11101 catcgctgtc tgcgagggcc agctgttggg ctcgcggttg aggacaaact cttcgcggtc
    11161 tttccagtac tcttggatcg gaaacccgtc ggcctccgaa cggtactccg ccaccgaggg
    11221 acctgagcga gtccgcatcg accggatcgg aaaacct
//
