LOCUS       sequence-208719-       10489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HA tagged UBE3B deletion of HECT domain in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Braganza A, Li J, Zeng X, Yates NA, Dey NB, Andrews J, Clark J, 
            Zamani L, Wang XH, St Croix C, O'Sullivan R, Garcia-Exposito L, 
            Brodsky JL, Sobol RW
  TITLE     UBE3B is a Calmodulin-Regulated, Mitochondria-Associated E3 
            Ubiquitin Ligase.
  JOURNAL   J Biol Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 
            10.1074/jbc.M116.766824.
  PUBMED    28003368
REFERENCE   2  (bases 1 to 10489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 
            10.1074/jbc.M116.766824."
COMMENT     Sequence Label: pLVX-HA-UBE3B-Delta-HECT-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..10489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2200..2503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2504..2707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2658..2678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2704..2728
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2823..2847
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2871..2897
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(5182..5206)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(5238..5812,5813..5815,5816..5824)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5405..5422)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5632..5651
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5844..6443
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5844..5863)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6340..6360
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6457..7045
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6510..6530)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6928..6939)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7252..7885
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8013..8029)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8013..8029)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8026..8048)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8037..8053
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8061..8067,8068..8085,8086..8091))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8106..8127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8244..8261)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8415..9003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8495..8514)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9174..9965,9966..10034))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9797..9816
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10035..10139)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10187..10321
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10224..10243)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10278..10297
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10489,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gataagcttg ggagttccgc gttacataac ttacggtaaa
     2221 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2281 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2341 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2401 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2461 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     2521 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2581 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2641 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2701 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2761 ccatagaaga caccgactct actagaggat ctatttccgg tgaattcctc gagactagat
     2821 caacaagttt gtacaaaaaa gcaggctccg cggccgcccc cttcaccatg tacccatacg
     2881 atgttccaga ttacgctttc accctgtctc agacctcgag agcatggttc atcgatagag
     2941 cccgtcaggc acgagaagaa aggcttgtgc agaaggaacg ggagcgggca gctgttgtga
     3001 tccaggccca tgtccggagt tttctctgtc ggagtcgact gcagagagat atcaggagag
     3061 agattgatga cttttttaaa gcagatgacc ctgagtccac taaaagaagt gcactttgta
     3121 ttttcaagat tgccaggaaa ctgctgttcc tattcagaat caaagaggat aatgagagat
     3181 ttgagaagtt gtgtcgcagc atcctgagca gcatggatgc tgagaatgag cctaaggtgt
     3241 ggtatgtgtc cctggcttgt tctaaggacc tcaccctcct ttggattcaa cagatcaaga
     3301 acattttgtg gtactgctgt gattttctca agcagctcaa gcctgaaatc ctgcaggact
     3361 cccgactcat caccctgtac ctcacgatgc ttgtcacctt cacagacact tcaacgtgga
     3421 aaattcttcg gggaaaaggt gaaagtcttc gaccagcgat gaaccacatt tgtgcaaata
     3481 taatgggaca tctcaaccag catggatttt attctgtgct gcagatattg ttaacccgtg
     3541 gcctggcaag accccgtcct tgtctatcca aaggcacttt aacagcagct ttttctctag
     3601 cgttacgccc tgtgattgct gcacagttct cagacaatct gattcggccg ttcctcatcc
     3661 acatcatgtc tgtgcctgct ctggtgactc atctcagcac agtgacccct gagcgcctca
     3721 ctgttttaga atcccatgac atgcttcgta aattcatcat atttttaaga gaccaagatc
     3781 gatgccgtga tgtatgtgaa agtttagaag gatgccatac gctttgtcta atgggcaacc
     3841 tcctacactt gggctccctc agccccagag tgttagagga ggagacagat gggttcgtga
     3901 gtttgctcac ccagacgctg tgctactgtc ggaagtatgt gtctcagaag aagtccaacc
     3961 tgacccactg gcatcctgtc cttggctggt tctcccaatc tgtggattat ggccttaacg
     4021 agtcaatgca cttgatcacc aaacagctgc agttcttgtg gggggtgcct ctgatccgga
     4081 tcttcttctg tgacatcctg agcaagaagc tactggagag ccaggagcca gcccacgcac
     4141 agccagcatc ccctcagaat gtgctcccag tgaagagtct cctaaagcgt gcttttcaaa
     4201 agtcggcatc agtccggaat attctcaggc ctgtcggggg taaacgggtc gactctgcag
     4261 aagtccagaa ggtttgcaac atctgtgtcc tctaccagac ctcgctgaca actctcacac
     4321 agattcggct gcagatactc acaggtctca cttaccttga tgacctgctt cccaaactgt
     4381 gggcatttat ctgtgagctc gggccccacg gagggttaaa gctcttcttg gaatgcctga
     4441 acaatgacac tgaagagtcc aagcaactct tggccatgct gatgctgttc tgtgactgtt
     4501 cgcggcacct catcacaatc cttgatgaca ttgaagttta tgaagaacag atttcattca
     4561 aactggaaga gctggtcact atctcctctt tcctgaattc ttttgtgttt aagatgatct
     4621 gggatggaat tgtagagaac gccaagggtg agaccttgga gctgttccag tctgtccacg
     4681 ggtggcttat ggtgctgtac gagcgggact gccggcggcg cttcaccccc gaggaccact
     4741 ggctgcgaaa ggatctcaaa cctagcgtgc tcttccaaga actcgacagg gacagaaaac
     4801 gggcacagtt gatcctgcag tacatcccac atgtcatccc tcacaaaaac agagttctac
     4861 tgtttcgaac catggttacc aaggagaagg agaaactggg gctggtggaa accagctctg
     4921 cctccccgca tgtcactcac atcaccatcc gccggtccag gatgctggag gacggctacg
     4981 agcagcttag gcagctctcc cagcacgcca tgaagggggt catccgtgtg aagtttgtca
     5041 atgacctcgg ggtggacgaa gcagggattg atcaagacgg tgtttttaag gagttcttgg
     5101 aagagatcat caagagagtt tttgacccag cactcaatct gttcaagaca accagtgggg
     5161 attagaaggg tgggcgcgcc gacccagctt tcttgtacaa agtggttgat ctagttctag
     5221 agcggccgcg gatcccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc
     5281 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     5341 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     5401 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     5461 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     5521 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     5581 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     5641 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     5701 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     5761 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata agcttgccac
     5821 aacccacaag gagacgacct tccatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     5881 gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     5941 cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     6001 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     6061 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     6121 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     6181 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     6241 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     6301 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     6361 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     6421 tgacccgcaa gcccggtgcc tagacgcgtc tggaacaatc aacctctgga ttacaaaatt
     6481 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6541 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6601 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6661 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6721 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6781 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6841 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     6901 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6961 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7021 atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac
     7081 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     7141 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     7201 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc
     7261 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     7321 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
     7381 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
     7441 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     7501 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
     7561 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
     7621 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
     7681 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     7741 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7801 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7861 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     7921 taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta gcgtttaccg
     7981 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     8041 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     8101 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     8161 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     8221 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     8281 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     8341 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     8401 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8461 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8521 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8581 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8641 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8701 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8761 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8821 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     8881 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8941 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9001 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9061 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9121 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9181 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9241 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9301 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9361 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9421 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9481 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9541 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9601 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9661 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9721 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9781 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9841 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     9901 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     9961 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10021 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10081 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10141 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc
    10201 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    10261 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    10321 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
    10381 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
    10441 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagtt
//
