LOCUS       sequence-208745-        7821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression of vsv-INCENP wt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Liu D, Vader G, Vromans MJ, Lampson MA, Lens SM
  TITLE     Sensing chromosome bi-orientation by spatial separation of aurora B 
            kinase from kinetochore substrates.
  JOURNAL   Science. 2009 Mar 6;323(5919):1350-3. doi: 10.1126/science.1167000. 
            Epub 2009 Jan 15.
  PUBMED    19150808
REFERENCE   2  (bases 1 to 7821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1167000"; journalName: "Science"; date: "2009-03-6-
            6"; volume: "323"; issue: "5919"; pages: "1350-3"
COMMENT     Sequence Label: pcDNA4-Tet-on-vsv-Incenp
FEATURES             Location/Qualifiers
     source          1..7821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             999..1031
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     primer_bind     complement(3829..3846)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3835..4059
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4105..4533
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4192..4211)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4402..4423
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4542..4562)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4547..4876
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4727..4862
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4789..4808
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4924..4971
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4990..5364
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5494..5615
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5531..5550)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5585..5604
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5664..5680)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5664..5680)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5677..5699)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5688..5704
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5712..5718,5719..5736,5737..5742))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5757..5778
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5895..5912)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6066..6654)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6146..6165)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6825..7616,7617..7685))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7448..7467
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7686..7790)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg ccaccatgta caccgacatc gagatgaacc
     1021 ggttgggcaa ggggacgacg gccccagggc ccattcacct gctggagctc tgtgaccaga
     1081 agctcatgga gtttctctgc aacatggata ataaggactt ggtgtggctt gaggagatcc
     1141 aagaggaggc cgagcgcatg ttcaccagag aattcagcaa agagccagag ctgatgccca
     1201 aaacaccttc tcagaagaac cgacggaaga agagacggat ttcttatgtt caggatgaaa
     1261 acagagatcc catcaggaga aggttatccc gcagaaagtc tcggagcagc cagctgagct
     1321 cccgacgcct ccgcagcaag gacagtgtag agaagctggc tacagtggtc ggggagaacg
     1381 gctccgttct gcggcgtgtg acccgtgctg cggctgcagc tgccgcggct accatggcat
     1441 tggctgcacc ttcttcaccc acccctgagt ctcccacgat gctgactaag aagcccgagg
     1501 ataaccacac ccagtgccag ctggtgcctg tggtggagat cggcatcagt gagcgccaga
     1561 atgctgagca gcatgtcacc cagctcatgt ccaccgagcc tctgccccgc actctgtccc
     1621 cgactccagc ttcagccaca gctccaacct cccagggcat cccgacatca gatgaggaat
     1681 caacacctaa gaagtcgaag gccaggatac tggagtccat cacagtgagc tccctgatgg
     1741 ctacacccca ggaccccaag ggtcaagggg tcgggacggg gcggtctgcg tctaagctca
     1801 ggattgcgca ggtctcccct ggcccacggg actcgccagc ctttccagat tctccatggc
     1861 gggagcgggt gctggctccc atcctgccgg ataacttctc cacgcccacg ggctctcgca
     1921 cggactctca atcggtgcgg cacagcccga tcgccccgtc ttccccgagt ccccgagtct
     1981 tagcccagaa gtactctctg gtggccaaac aggaaagtgt tgtccgcagg gcgagcagaa
     2041 ggcttgccaa gaagactgcc gaagagccag ctgcctctgg ccgcatcatc tgtcacagtt
     2101 acctggagag gctcctgaat gttgaggtgc cccagaaagt tggttctgag cagaaggaac
     2161 cccccgagga ggctgagcct gtggcggcag ctgagccaga ggtccctgag aacaacggaa
     2221 ataactcgtg gccccacaat gacacagaga tcgccaacag cacacccaac ccgaagcctg
     2281 cagccagcag cccggaaaca ccctctgcag ggcagcaaga ggccaagacg gaccaagcag
     2341 atggacccag agagccaccg cagagtgcca ggaggaagcg cagctacaag caggccgtga
     2401 gtgagctgga cgaggagcag cacctggagg atgaggagct gcagcccccc aggagcaaga
     2461 ccccttcctc accctgccca gccagcaagg tggtacggcc cctccggacc tttctgcaca
     2521 cagtgcagag gaaccagatg ctcacgaccc cgacctcagc cccacgcagc gtcatgaagt
     2581 cctttattaa gcgcaacact cccctgcgca tggaccccaa gtgcagcttc gtcgagaagg
     2641 agcggcagcg cctggagaat ctgcggcgga aggaggaggc cgagcagctg cgcaggcaga
     2701 aggtggagga ggacaagcgg cggcggctgg aggaggtgaa gctgaagcgt gaggaacgcc
     2761 tccgcaaggt gctgcaggcc cgcgagcggg tggagcagat gaaggaggag aagaagaagc
     2821 agattgagca gaagtttgct cagatcgacg agaagactga gaaggccaag gaggagcggc
     2881 tggcagagga gaaggccaag aaaaaggcgg cggccaagaa gatggaggag gtggaagcac
     2941 gcaggaagca ggaagaggat gcacgtaggc tcaggtggct gcagcaggag gaggaagagc
     3001 ggcggcacca agagctgctg cagaagaaga aggaagagga gcaggagcgg ctgcggaagg
     3061 cggccgaggc taagcggctg gcagagcagc gggagcagga gcggcgggag caggagcggc
     3121 gcgagcagga gcggcgcgag caggagcggc gggagcagga gcggcgcgag caggagcgac
     3181 agctggcaga gcaggagcgt cggcgggagc aggagcggct ccaggccgag agggagctgc
     3241 aggagcggga gaaggccctg cggctgcaga aggagcagct gcagagggaa ctggaggaga
     3301 agaagaagaa ggaagagcag cagcgtctgg ctgagcggca gctgcaggag gagcaagaga
     3361 agaaagccaa ggaggcagca ggggccagca aggccctgaa tgtgactgtg gacgtgcagt
     3421 ctccagcttg tacctcatat cagatgactc cgcaagggca cagggcccct cccaagatca
     3481 acccagataa ctacgggatg gatctgaata gcgacgactc caccgatgat gaggcccatc
     3541 cccggaagcc catccccacc tgggcccgag gcaccccgct cagccaggct atcattcacc
     3601 agtactacca cccaccgaac cttctggagc tctttggaac cattctccca ctggacttgg
     3661 aggatatctt caagaagagc aagccccgct atcacaagcg caccagctct gctgtctgga
     3721 actcaccgcc cctgcagggc gccagggtcc ccagcagcct ggcctacagc ctgaagaagc
     3781 acaagctttg actcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     3841 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     3901 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     3961 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     4021 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     4081 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4141 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4201 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4261 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4321 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4381 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4441 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     4501 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     4561 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4621 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4681 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     4741 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4801 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4861 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     4921 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     4981 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     5041 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     5101 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     5161 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     5221 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     5281 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     5341 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     5401 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     5461 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     5521 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5581 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     5641 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5701 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5761 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     5821 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5881 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5941 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6001 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6061 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6121 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6181 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6241 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6301 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6361 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6421 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6481 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     6541 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6601 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6661 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6721 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6781 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6841 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6901 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6961 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     7021 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     7081 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     7141 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     7201 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7261 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7321 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7381 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7441 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7501 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7561 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7621 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7681 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7741 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7801 cgaaaagtgc cacctgacgt c
//
