LOCUS       sequence-208749-       13933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses pentamerised full-length protein ectodomain with no 
            N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 
            d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13933)
  AUTHORS   Knockel J, Dundas K, Yang ASP, Galaway F, Metcalf T, Gemert GV, 
            Sauerwein RW, Rayner JC, Billker O, Wright GJ
  TITLE     Systematic Identification of Plasmodium Falciparum Sporozoite 
            Membrane Protein Interactions Reveals an Essential Role for the p24 
            Complex in Host Infection.
  JOURNAL   Mol Cell Proteomics. 2021;20:100038. doi: 10.1074/mcp.RA120.002432. 
            Epub 2021 Jan 27.
  PUBMED    33515807
REFERENCE   2  (bases 1 to 13933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2021"; volume: "20:100038. doi"; pages: " 
            10.1074/mcp.RA120.002432. Epub 2021 Jan 27"
COMMENT     Sequence Label: CTRP-COMP-blac-flag-his
FEATURES             Location/Qualifiers
     source          1..13933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..329
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      352..361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4198..4212
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             7465..8250
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             8257..8322
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             8329..8346
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    8384..8439
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8438..8457)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(10675..10693)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10761..10865
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10866..10934,10935..11726)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11897..12485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12386..12405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12639..12656
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12773..12794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12809..12814,12815..12832,12833..12839)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12847..12863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12852..12874
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12871..12887
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12871..12887
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        13158..13537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        13538..13741
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     13692..13712
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     13738..13762
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgcccac
      421 tacgccaaga acgaggaaac aaacaagaag gacaccaagc tgaagaagaa gcggaacgtc
      481 aagaccttca acaagaagct gaccaacaag gccttcctgc aggtccagca caccgtggcc
      541 accagaagcg tgccacctcc accctgcctg ggcgacgact gcttctgcca gaactactac
      601 gacctgaccc tgatcctgga cgagagcgcc agcatcggca gcaagaactg gaagaaccac
      661 gtgatccctt tcaccgacaa gatcatcaag gacctgacca tcagcaagaa tgaggtgcac
      721 gtgggcatcc tgctgttcag ctccaagaac cgggactacg tgacctacgg cgacgagctg
      781 agataccaga aggacgagct gctgaagaaa gtcgagaagc tgaaaaagga ctactactgc
      841 ggcggaggca ccaagatcct gggcgccctg aagtacagcc tggaaaatta cgccaagcac
      901 aagaacatcc gctacgacgc ccctaaagtg accatcctgt tcaccgacgg caacgagaac
      961 agcgcctcca acaaacagct gctggaaatg ggcctgacct acagaagaga gcgggtcaag
     1021 ctgctggtgc tgggcgtggc cgctgccgag gataacaagc tgaaactgat cgccggctgc
     1081 gaggaaaaca ccaactgccc ctacagcatg aaggccgagt gggagacaat caacgacatc
     1141 accaagcggc tgactaacaa gatctgccac accgagagcg agatcgagcc cgagcccaag
     1201 cctgagccct ccacccctac acactgtcag ggtgatgatt gcttctgcga ggattactat
     1261 gatctcacac tcatcctgga tgaatcccgg tctatcaccc tggacaagtg gaagaaagac
     1321 gtggtgccct tcgccgagaa ggtgctgaac aacctgaaca tcgacaagga caagatccac
     1381 gtgggaatca tgagattcgc caagtccatg aagaccgaca tcggctacga gcaggaaacc
     1441 cggtacatga agaacgacct gatcaagctc gtgcgcgagc tgaaggataa gtacggctac
     1501 ggcggagcca cccacctggt ggacgccctg cagtactccc tgaaaacctt cacccggcac
     1561 cccaacaaca gagtggatgc tcccaaagtc acaatcctct tcacagatgg caatgaggcc
     1621 agcaagaaag agaaggacat ccgggacgtg ggcctgctgt accggaaaga aaacgtgaag
     1681 ctgatcgtcg tgggcgtgaa cctggccacc gagaaaagcc tgaaactgct ggccggatgc
     1741 accgagaacg aagagtgcct gagagtgatc aagtgcgagt ggaacgatct gaccaatatc
     1801 gccaagattc tgaccgacaa aatctgcaat accggcagcg tggaactgcc caagcccgaa
     1861 gagaaccccg agcccgtgga aaagcctaac cctgaagaga atcccaaccc cgtcgagaaa
     1921 cccaccccag aagaaaaccc aaaccctgtg gaaaaaccta cacctgagga aaaccctaat
     1981 ccagtcgaaa agccagagcc cgagaagaac ccttgcatca acatggaaga ttgctactgc
     2041 aaggacttct acgacattac cctggtgctc gacgagtccg cctccatcag cgatctgatc
     2101 tggcggaacg aagtgatccc cttctccctg gaaatcatca agcggatcaa cattgcctac
     2161 aagaacgtgc acatgggagt gctgctgttc tccgagtaca cccgggacat cgtgcggttc
     2221 tacgataacg ccagatacga gaagggcaca ctgcagacca agatcaatga cctgaagcgg
     2281 gactacagaa gcggcaaaaa gacctacatc atccaggctc tgcagtacgc tctgacctac
     2341 tacagcaagc tgagcaacag aaaagaggcc ccaaaagtca ctatgctgtt taccgatgga
     2401 aacgacgcct acgagtctga gaagggcctg caggatatcg ccctgctcta tcgcaaagag
     2461 aacgtcaaac tcctggtcgt gggagtgtcc accgccagcg agaacaaact gaagatgctc
     2521 gtgggctgcg cccctaacgt cgtgtgcccc ttcgtgatca agacagagtg gggactgctg
     2581 aaaaacgtgg ccgaggtgtt cgtgaagaag atctgcgaca acggcgtggt gctgcctccc
     2641 ggcagcccta gcgagtctac acctggcagc ccctccgaga gcacccctgg ctctccaagc
     2701 gagagcacac ccggaagccc cagcgaaagc accccaggca accctgccga gtccacacca
     2761 ggctccccat ccgaatccac tcccggcaac cccgctgaga gcactccagg cagcccatct
     2821 gaatcaaccc caggatcccc aagcgagtcc accccttgca gcggcaccga gtgcctgtgc
     2881 cacaacacct atgatctgac tctgattatc gatgagagcg cctctatcgg ctactccaac
     2941 tgggagaaag aggtggtccc attcaccatc ggcctggcca gcaatctgga aatctctgag
     3001 aagaaagtga acatggggat tctgctcttc agcgataaga tccgcgagtt catcaaatac
     3061 ggccagaaag agagctacga caagaacaac ctcgtgcggc ggatccacga cctgaagaag
     3121 tactacaaga gcggcggctt cagctacatc gtggaagccc tgaaatatgg cctgtacagc
     3181 tatgccaaga gcaccagctc ccggctgaac gtgcccaaag tgaatatcct gctgacagac
     3241 ggaaacaacg ccgacaccag cgacttcatc ctgaccgagg tgtccagcct gtacaagaaa
     3301 gaaaatgtca aactgctgct gatcggcatc ggcggaccca ccatccacaa gctgaggctg
     3361 ctgggcggct gcgacaagtc cgacggcgac tgcccttacg tggtcaaggc cgaatggaac
     3421 aatctcaagt acaccagcaa cctcatcatt gacaagattt gtcacacaga caagccagtg
     3481 gaaaaccccg gcgacaacag cgccgtgtgc aacagcaagg acgattgcat ctgcgccaac
     3541 tacttcgatc tgacttacat cgaggtgccc accctgaact ccgcctacac atggcggagc
     3601 gactttatgg actgctctaa gaacatcatg aacagcctcg tgatcgataa gaacaaagtg
     3661 cacgtgtccc tgatcatcag cctcgagaaa aagagcgtgc accagggctt cgatgacgtg
     3721 aacagctaca acaaaaacga gctgattaag accctgggca agctcgagaa tagcgccgtc
     3781 ctgaacaagg ccaacatcct ggactccctg gtgtacggca tccagcagag cttcggcaag
     3841 ggcaaccgcg agaacgctcc aaaagtgaca atgctcctga ccaactccaa cagcgacatc
     3901 agcgacgaga aagccctgca ggacatctac ctgaattaca aagagaagtc catcaagctc
     3961 ctcatcatcg ggatcggaat caccaacacc ggcaagctgt tcaacgctgg cggctgcaac
     4021 atgaacggcg acaattgccc tcacgtgtac gccagcaagt ccttctccta catcggcggc
     4081 gtggacacct tcctggaagt gaacaagtgt gacagcagca tcgacaacaa cggcggcgat
     4141 aacggcaacg gcgccgaggg caacgtgaac cccgacaccc ctagctgcaa cgacaacgac
     4201 gacgatgaca agtgcaacaa cgatgacagc aacatcatct gcaccaaggt gctggatatc
     4261 gctgtggtcc tggaccagtc cagcaatatc gctaaggacc agtggaacgt gtacatcaag
     4321 cagttcgtca tcaacaccgt gaaccagaat tacctgagca agtaccggtc ccacatcaca
     4381 atcgtgaaga tgggcaagtc caccaaagag aaatggtccc tgaacaaaaa gatcagctac
     4441 cagaagaaga aaattatcaa agaaatcaac aagctgccca tctcctactc caagaagaag
     4501 gatattgcca agtccctcaa atacgtgcgg accaaggtgt tcaagaagtc cgagacaaac
     4561 cggaagaaac tcatcatcat gctggtcgag ggcaagagca actccaatat gaatgacctg
     4621 cggaaagaag tcggcctgct gaaagtcaac aacatcgatt tcttcgccta cgccatcgac
     4681 aatatcgacg agacagagta taagatcctc ggcgattgcg agggctccgt ggacatggga
     4741 ctgatgggca acagccctcc cagccccagc tacctgccct gcaagaatat cgtgaaagtg
     4801 tcctgggaca ccctgctgag cagcaccgac atccacatga agtacatctg caacggctac
     4861 cccgaggacg ccgagtgcag cgagtgggaa gagtggtccc cttgccccga gacatgcccc
     4921 accatcaata acaacaacaa taacgccgct ggcagcaaca atgccgccag gaccaacatc
     4981 agaacctact tccctcagct gtccaagaga gagcggaagg gcccatacac cctgaagggc
     5041 gaagagtaca tgggcgagaa gtacggcagc agctgcatgg aactgaagtc tatcgagtac
     5101 agaagctgcc ccatcaacgc cggctgtaac gacatgtgcg gcgacttcgg cgagtggtcc
     5161 gagtgctctg ccacctgtgg cgagggcatc agagtgcgga acagagacaa tagcctggac
     5221 aatgacgata agtgcaagct ctttaactcc gccgagatgg aagcctgcaa catccaggaa
     5281 tgcgacgaca ataacaatgt ggacatttgc gaggacattg gggagtggtc tgactggtcc
     5341 tcctgcagca agacctgcgg ctacagcacc cggtcccgga ccttcaccat cctgcccgag
     5401 tatatcggcg agtaccccaa ctgcaagatc ttcgagcgga gcgagacaga agtgtgcgcc
     5461 ttcatccccg cctgctccga cgagaactgc tttgagtggg aggaatggaa cgagtggtct
     5521 agcccctgca gccccagaaa gcgggtgcag aaagcccggg tgctcaaaaa ggacgacgtg
     5581 atcatctcca gcggggataa caacaacaac aacaataaca acgccaagcg gggcatgggc
     5641 cacaagaatt ccgccttcac cagctataac aataagaagt ctgacatctg cgaagaggaa
     5701 gtgcggcact acctggataa ggtggaatac gacgaagagt ccacctgtga aaacaagaac
     5761 ccctgcggag attggagtga ttggagcgag tgcgaccgga cctgcaatgt gggcgtgcgg
     5821 atccggcact tcatcagcca catgttcgac atggtcggag atgaggatga gaaagagtgt
     5881 ctcgagtact ataacaaggt ggaaacccag gactgcctgc atctgcctcc ctgcgacggc
     5941 ggagagtgct ccgactggga aacctgggtg gaatgcaaag aagaggacat gatcggcaac
     6001 aactgccaca agcggaacaa gaagatcctg accagaaagc tggaactcct gaagaacaag
     6061 gacatcaccc ggaccaagaa caccgccgac gtgtgcaatg actacaccct gttccgggaa
     6121 gaggactgcc ctcagagcaa tgacgcctgc atcaatgccc tgtgcaacga atgggaagaa
     6181 tggggcgact gcagcagcac atgcggcgag ggaagcttca agatccggaa gcggaaagag
     6241 cccctggaac tgatccccgc cagccaggac atcaacggca atatcggcct gacatgtgcc
     6301 cagcagaaca tcaaggtcga ggaacgcgag gcctgtatcg tgcccgcctg cgaggacgag
     6361 tctaccaacg gcggaaccga ggtggaaggc tccaccccaa gcagcccaag tgactctaac
     6421 aacaatgacg gcagctccgg cgataatacc ggcgactcca acgataagaa aggcatgggc
     6481 accggcgaaa aggtgtccct ggccgctggc gtgatcggac tggtggctct ggcagctggc
     6541 ggcctgatct acggctacaa taccctgaat ggcggcgagc ctccccacag cagcaatatg
     6601 gaattcgaga acgtggaaaa caacgcaggc accgaggaag aggaaaacga ggacttcgag
     6661 gtggtggatg ccgacgaccc catgtggaac ggcgcgccgt cgacctccat cacggcctat
     6721 aagagtgagg gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg
     6781 cagggagagt tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc
     6841 tccctaaaga accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg
     6901 tccgaaacgc tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct
     6961 ggcaacctga ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg
     7021 atgaaagtga ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca
     7081 cccaagatga gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag
     7141 aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt
     7201 gaagaggtca agatggactc caagatccag gttttatcca aagggttgaa ttccggcggc
     7261 ggctccggag gcggtaccgg tggcggcgac ctagccccac agatgcttcg agaactccag
     7321 gagactaatg cggcgctgca agacgtgaga gagctcttgc gacagcaggt caaggagatc
     7381 accttcctga agaatacggt gatggaatgt gacgcctgcg gtctagacag aaacctgcct
     7441 ccactagcac ctctgggccc acacccagaa acgctggtga aagtaaaaga tgctgaagat
     7501 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7561 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7621 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7681 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7741 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7801 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     7861 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     7921 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7981 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     8041 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     8101 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     8161 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     8221 gagataggtg cctcactgat taagcattgg ggccctgact acaaagacca tgacggtgat
     8281 tataaagatc atgacatcga ttacaaggat gacgatgaca agggccctca ccaccaccat
     8341 caccactaat taattaagga tcccccgacc tcgacctctg gctaataaag gaaatttatt
     8401 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     8461 gcaaatcatt tggtcgagat ccctcggaga tctctagcta gaggatcgat ccccgccccg
     8521 gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt gcatgtaatc
     8581 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg
     8641 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat
     8701 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac
     8761 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt
     8821 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg
     8881 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc
     8941 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta
     9001 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag
     9061 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta
     9121 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa
     9181 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt
     9241 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa
     9301 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg
     9361 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat
     9421 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac
     9481 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg
     9541 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta
     9601 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt
     9661 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga
     9721 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc
     9781 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc
     9841 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct
     9901 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt
     9961 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg
    10021 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata
    10081 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
    10141 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
    10201 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
    10261 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta
    10321 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat
    10381 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat
    10441 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
    10501 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
    10561 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
    10621 atgctatcct catgataagc tgtcaaacat gagaattttc ttgaagacga aagggcctcg
    10681 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
    10741 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    10801 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    10861 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    10921 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    10981 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    11041 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    11101 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    11161 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    11221 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    11281 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    11341 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    11401 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    11461 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    11521 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    11581 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    11641 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    11701 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    11761 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    11821 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    11881 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    11941 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    12001 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    12061 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    12121 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    12181 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    12241 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    12301 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    12361 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    12421 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    12481 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    12541 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    12601 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    12661 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    12721 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    12781 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    12841 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    12901 agctctagct agaggtcgac caattctcat gtttgacagc ttatcatcgc agatccgggc
    12961 aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct atacattgaa
    13021 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
    13081 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
    13141 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
    13201 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
    13261 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
    13321 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
    13381 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
    13441 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
    13501 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
    13561 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
    13621 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
    13681 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
    13741 tcgtttagtg aaccgtcaga tcctcactct cttccgcatc gctgtctgcg agggccagct
    13801 gttgggctcg cggttgagga caaactcttc gcggtctttc cagtactctt ggatcggaaa
    13861 cccgtcggcc tccgaacggt actccgccac cgagggacct gagcgagtcc gcatcgaccg
    13921 gatcggaaaa cct
//
