LOCUS       sequence-208755-       12463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses pentamerised full-length protein ectodomain with no 
            N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 
            d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12463)
  AUTHORS   Knockel J, Dundas K, Yang ASP, Galaway F, Metcalf T, Gemert GV, 
            Sauerwein RW, Rayner JC, Billker O, Wright GJ
  TITLE     Systematic Identification of Plasmodium Falciparum Sporozoite 
            Membrane Protein Interactions Reveals an Essential Role for the p24 
            Complex in Host Infection.
  JOURNAL   Mol Cell Proteomics. 2021;20:100038. doi: 10.1074/mcp.RA120.002432. 
            Epub 2021 Jan 27.
  PUBMED    33515807
REFERENCE   2  (bases 1 to 12463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2021"; volume: "20:100038. doi"; pages: " 
            10.1074/mcp.RA120.002432. Epub 2021 Jan 27"
COMMENT     Sequence Label: CCp1-COMP-blac-flag-his
FEATURES             Location/Qualifiers
     source          1..12463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..329
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      352..361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5995..6780
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             6787..6852
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6859..6876
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6914..6969
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6968..6987)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9205..9223)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9291..9395
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9396..9464,9465..10256)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10427..11015
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10916..10935
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11169..11186
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11303..11324
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11339..11344,11345..11362,11363..11369)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11377..11393
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11382..11404
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11401..11417
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11401..11417
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        11688..12067
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12068..12271
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12222..12242
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12268..12292
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgcccag
      421 gaaagcgcca ccaacttcta caagttcatc gacagcttcg ccagcagcac ctacatgagc
      481 gaggaaagcg gcagcagcgc ctacgacgcc aagcgggcca tccagaacaa ccccaactac
      541 tggtgcagct ccggcaacca cgccaacgac gaggaaatca cctggaccgg ctacctgaac
      601 accaagggct tcatcaaggg cgtgaaggtg tcctgggcct acagccccga gttcgtgaag
      661 atcagcgtgt ccagcgacgg cgagaagtac cggaccatca tcccctacaa gaagatctcc
      721 agcaacgagg ccagcttcga cgagatctac ttcttcaagc ggctggaaga ggccatgagc
      781 atcaagatcg gcctgaagaa cgcccggcac aagtacttcg gcatccggga agtgaagctg
      841 atcggcggag gcaaccccta cttcctgctg ctgagcggca tcagctccga ggaagagatg
      901 tgcctgcagg tcgaggaagg cctgatcaac aacgacaaca ccgccatcat cctggacagc
      961 tgtaccaacg ccctggcctc cggcgacggc agagagctgt ggaaaacaaa cagcaacaac
     1021 caggtcatca gcgccttcag cgaccctccc aagtgcctga gcgtggtcaa cctggacgac
     1081 ctcgagaaca acaagatcgt gctgtacgac tgcctgcggg ccctggaaga tggcgacgga
     1141 aagagcaact ggatcttcga gagcaacagc cagatccggc tgcagaagtc tggcgacgcc
     1201 ttctgcatca gccagaagaa catctacggc aacatccccg gcatccacga catcctgctg
     1261 aacctggatg tgtccatcta cagcaactcc gccctggacg atgaccacaa ccccgacaat
     1321 accatcgacg gcaacctgaa ctcctactgg gccagcgcca ccttcaccga caactacgac
     1381 cacctggtgt acctggtgct ggacctgaac aagatcaccg acctgagccg gatcaagatc
     1441 tactgggagt accctcccct gcactacaat atcgccgtgt ccaccgacaa tcagaacttc
     1501 gccgtggtgt ccgagaacct ggccaacccc gcctacatca ccgtggactc cctgaagaat
     1561 atggaaaccc ggtatatcaa gatcagcatg atcaagaccc accccaagca cggcgagctg
     1621 ggcgacaact tcctgtacgg catccgcagc atcgaggtgc aggccaacaa cctggaaacc
     1681 gtgatcaacc actgccggga cgccgccaac agcgacgacg ccagagacaa gtatttcgtc
     1741 gagtacatca cagagttcga caaggacctg accaacaagc tgatcaacct cgaggacgac
     1801 gtgaccaaga acgtggccag catcagcgac aatctggcca agctggaaga actgctgccc
     1861 aacatcgaga catgcctgga agagaagaaa acctacgatg aggaactgaa agagagcaaa
     1921 gagaaggcca acgacctcaa caacaaactg agcctgctga ccagcgtgaa cgtgaacacc
     1981 ctggactccg atatcctgaa gctgggcatc ctgcccggcg acagctacaa cttccccgcc
     2041 aacgattgcg ccgtgattaa gaacgtgcag gaaaaccctc tgagcggctt ctactggatc
     2101 aagcccaagt gcagccctga gcccctgcgg gtgtactgcg acatggacag ctccaccagc
     2161 atctacatct ggaacggcaa cccacccaag agccccgatc acctgatcac caacatgatc
     2221 aactccgtga acgacatccg gcagcactgc gccgaagtgg gcctgcagcc tctgatcctg
     2281 agaagcaaga accagctgaa cagcctgatc atctctctga agaagatcgg ctacagcctg
     2341 aacggcaaag tgaacatccc tctggcctac gactacagct gcgaccacgg cagctgcagc
     2401 ggcagattcc acgacctgct gaatggcaac atcgacatca gcaccctgat ctacctgaag
     2461 gccagcgaga gccctgacag caccaaagtg cggcagaccg ccggcatctc ctacgacgac
     2521 ggctccttca agttcttcaa tctggaaacc agcgacatct ccgccatcgt gtgcagcacc
     2581 aacagcgccg agaacgacgc cgccctgcag tacctgtcca tcaactgcga gacaaccggc
     2641 atggaagata gcttccacag catcgtgaat accaatatcg tggtgctgtg ccctctgggc
     2701 tgcgacgatg agaagtatca cgacgcctcc atctatggca gccggggcac ctacagcgat
     2761 aacagcgcca tctgcagagc cgccatccac tccgacatca tcgacaacaa gggcggcctg
     2821 gtcaatgtgg ccatcgagag cggcatggac cactacgtgg gcagcattaa caacaatatc
     2881 gagagcatct ccctcaacaa gaacgagaag ggcctgctgg acatcatccc tgaggaaaaa
     2941 gagggcacca acaacatcag agaggaaagc agcatcttcc accacaagac catccgggtg
     3001 tcctccctga tcgaggactg tcccctggac ctgttcctgt tcaaccaggc tagcttcctg
     3061 gaaaagggaa acaacatccg gaacaacaaa ggcaccgagc tgaagtacaa cgacgacgag
     3121 aacatggccg tgaagaattt ccacgagctg atctccaacc tgatggaaaa catcgatgcc
     3181 atccacggcg tggacagcag cgtgatcagc attgtgcagg aagagacaat ccggatcatc
     3241 gaaaagacca agaaagagct gaagcccgcc gacatgctga gcaagaagca gattgaggac
     3301 gccatgaacc tgtacaacct ggccgagaat ctggctatct acctgtacga tctgagcagc
     3361 aagtacatcc aggatctcga gaagctgaag aacaccctcg aggaactcaa gggcgcccag
     3421 aaggtggccc acaacttcgg caccttcaag ctgaactacg agacaatgaa tttcgccacc
     3481 cacttcagcc tgttcgacag caacctcatc aagaacaaag aaagcgtgtg gggctacagc
     3541 gacaccaaca tcctgggcca cgagaacagc atcggccaga tgaactccgt gtctagccag
     3601 gaaatcggcg agggctacta tgccaagctg aagggactga acttctacga cttcgacttc
     3661 aacattgccg tgctgagcag aggcaccggc tgtctgggcg tggtgttccg ggccaaggac
     3721 gacttcaatt tctacctgtt tgacatctgc gacaaggatg gcaccaagag actgagcaag
     3781 gtggaaaacg gccaggtgca catcctgaag aaggtggtca actctgacgt gaccctgaac
     3841 aaccagtgga acaagtacaa gatcatcacc aagcacgcca atatcgacat ctacgaggtg
     3901 gacaaggaca acaacatgat taagatcctg agcagcctgg acgagcggtt cctgtccggc
     3961 accgtgggcc tgtactctca gatctacggc ctgggcacct tctttgacga cctggaagtg
     4021 atcgccctgc cctgcaccca gctgagcgag ctgaacacac tgaacaaaaa cgtgaagtcc
     4081 aactgcccct actacaaaga gaactatctc aacaacctca tgagctacga tatcatctac
     4141 aaccctaaca actacttcaa ctggaacgtc gagaaagaga acgagcagaa ctacctgctg
     4201 tgcagcaaga acgaagagga agtgaaaaac gccaaggatg agaaggatat ctacaccatc
     4261 gtgctgctga agctgcgcga gtgcaccgac ggcaccttta acttcgatat tcaggtgtcc
     4321 gacgacgaaa ccgggaatat cgccaagaag ctgagctaca tctatatcct gttccactac
     4381 aaggacgaaa acaacttcaa cgccctcgag atgaaggacg gcaagctggc tttcctgaca
     4441 aacaagaatg gcaagagctt catcctgtcc gagcggaacg aggaagaaaa cgataacaac
     4501 aaaaacattg agaagcggtt caccttcgtg cagaacgaat ggatccacgt gaacctgcac
     4561 ttcgacaagt ccaccttcaa agtgatcatc atcacaaaca acaacgagga caagttcgtc
     4621 ctgagcgcca agagccggaa cgacgtgccc ctgggcaaag tgggcttcct ggtgcacaat
     4681 ttcgacgaag tgaagttcga ctccatcctg ctcaacagcc ccaccatcac aaaggtggac
     4741 gagaactttc tgcaggtcaa aagcaagacc tgggccaatt gcgaggacag cgtgcacgtg
     4801 ctgcaccggc ggttcagctg cgagactgac atctacccca atgaggccaa agaaaagcac
     4861 atcaagtgca tcaagaattt ctgcaaagag tgctgcctgt accacacaca gctgctggac
     4921 tccaacgaga agaacgagtg cgagaagcac tgcaagcaga acgataatct ggccgccaag
     4981 atgcagaccc tgttcgagaa gttcatcaat cgctgcgtgt ccctgaacga gaatgaggac
     5041 tacgaaacct gcgataagaa cgacaagaaa tgcaaaaaca aagtgtgcgt gctgtgctgc
     5101 aaaaagcacg accccaccac cagcaaagaa ctgaaggtgc tgcccatgaa ccagttcaag
     5161 aagattcagg aaaacgagat catcgagtgc cagctgcagt gcaatatgat ccactccatc
     5221 ggcgcgccgt cgacctccat cacggcctat aagagtgagg gggagtcagc ggagttctcc
     5281 ttcccactca accttggaga ggaaagcctg cagggagagt tgagatggaa ggcagagaag
     5341 gctccttctt cccagtcctg gatcaccttc tccctaaaga accaaaaggt gtctgtgcag
     5401 aagtctacta gcaaccccaa gttccagctg tccgaaacgc tcccactcac ccttcagata
     5461 ccccaggtct cccttcagtt tgctggttct ggcaacctga ccctgactct ggacagaggg
     5521 atactgtatc aggaagtgaa cctggtggtg atgaaagtga ctcagcccga cagcaacact
     5581 ttgacctgtg aggtgatggg acccacctca cccaagatga gactgatctt gaagcaggag
     5641 aatcaggagg ccagggtctc caggcaggag aaagtgattc aagtgcaggc ccctgaagca
     5701 ggggtgtggc aatgtctact gagtgaaggt gaagaggtca agatggactc caagatccag
     5761 gttttatcca aagggttgaa ttccggcggc ggctccggag gcggtaccgg tggcggcgac
     5821 ctagccccac agatgcttcg agaactccag gagactaatg cggcgctgca agacgtgaga
     5881 gagctcttgc gacagcaggt caaggagatc accttcctga agaatacggt gatggaatgt
     5941 gacgcctgcg gtctagacag aaacctgcct ccactagcac ctctgggccc acacccagaa
     6001 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     6061 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     6121 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     6181 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6241 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6301 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6361 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6421 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     6481 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6541 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6601 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6661 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6721 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6781 ggccctgact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat
     6841 gacgatgaca agggccctca ccaccaccat caccactaat taattaagga tcccccgacc
     6901 tcgacctctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
     6961 tgtctctcac tcggaaggac atatgggagg gcaaatcatt tggtcgagat ccctcggaga
     7021 tctctagcta gaggatcgat ccccgccccg gacgaactaa acctgactac gacatctctg
     7081 ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa cttgccaact
     7141 gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc tctgactgta
     7201 gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt tcatcctgga
     7261 gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa ctcttggctg
     7321 aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa gactacggga
     7381 ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga ccctcaagag
     7441 ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta gcatatgctt
     7501 cccgggtagt agtatatact atccagacta accctaattc aatagcatat gttacccaac
     7561 gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca gcgatatctc
     7621 ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg agggtagtga
     7681 accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga actctcctga
     7741 atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc tgagttgtga
     7801 acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa
     7861 atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac cctcacaaac
     7921 cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt aacaatagaa
     7981 atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga atttatggct
     8041 atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg tgtcccaggc
     8101 agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg gaaagagagt
     8161 ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa ttaaacgggg
     8221 ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt tgaaattgtg
     8281 gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg taaaataagg
     8341 gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca cttgcccaca
     8401 aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc caagataggg
     8461 gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc gccaagcaca
     8521 gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg
     8581 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     8641 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     8701 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     8761 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     8821 gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta tatctgggta
     8881 gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat
     8941 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     9001 atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat
     9061 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     9121 atgctatcct aatctgtatc cgggtagcat atgctatcct catgataagc tgtcaaacat
     9181 gagaattttc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     9241 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     9301 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     9361 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     9421 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     9481 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     9541 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     9601 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     9661 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     9721 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     9781 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     9841 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     9901 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
     9961 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    10021 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    10081 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    10141 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    10201 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    10261 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    10321 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    10381 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    10441 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    10501 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    10561 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    10621 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    10681 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    10741 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    10801 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    10861 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    10921 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    10981 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    11041 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    11101 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    11161 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    11221 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    11281 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    11341 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    11401 caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac caattctcat
    11461 gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc aggcgcagaa
    11521 ctggtaggta tggaagatct atacattgaa tcaatattgg caattagcca tattagtcat
    11581 tggttatata gcataaatca atattggcta ttggccattg catacgttgt atctatatca
    11641 taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac attgattatt
    11701 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
    11761 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
    11821 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
    11881 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
    11941 gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
    12001 gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
    12061 taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg
    12121 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
    12181 acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg
    12241 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcctcactct
    12301 cttccgcatc gctgtctgcg agggccagct gttgggctcg cggttgagga caaactcttc
    12361 gcggtctttc cagtactctt ggatcggaaa cccgtcggcc tccgaacggt actccgccac
    12421 cgagggacct gagcgagtcc gcatcgaccg gatcggaaaa cct
//
