LOCUS       sequence-208757-        9925 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses pentamerised full-length protein ectodomain with no 
            N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 
            d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9925)
  AUTHORS   Knockel J, Dundas K, Yang ASP, Galaway F, Metcalf T, Gemert GV, 
            Sauerwein RW, Rayner JC, Billker O, Wright GJ
  TITLE     Systematic Identification of Plasmodium Falciparum Sporozoite 
            Membrane Protein Interactions Reveals an Essential Role for the p24 
            Complex in Host Infection.
  JOURNAL   Mol Cell Proteomics. 2021;20:100038. doi: 10.1074/mcp.RA120.002432. 
            Epub 2021 Jan 27.
  PUBMED    33515807
REFERENCE   2  (bases 1 to 9925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2021"; volume: "20:100038. doi"; pages: " 
            10.1074/mcp.RA120.002432. Epub 2021 Jan 27"
COMMENT     Sequence Label: AAP-COMP-blac-flag-his
FEATURES             Location/Qualifiers
     source          1..9925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..329
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      352..361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3457..4242
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             4249..4314
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4321..4338
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4376..4431
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4430..4449)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6667..6685)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6753..6857
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6858..6926,6927..7718)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7889..8477
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8378..8397
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8631..8648
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8765..8786
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8801..8806,8807..8824,8825..8831)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8839..8855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8844..8866
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8863..8879
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8863..8879
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        9150..9529
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9530..9733
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9684..9704
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9730..9754
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccaag
      421 ggcaagagac agaacctggt gtccgcctac ctgaaccact tcaagaagtc ctacttcagc
      481 ggcgtgacca gcggcagcga ctgcgtgaac aaggccgagg tgtccagcga caacaacaat
      541 aacaataata acaacaacaa caagatcgcc cacaacttct tcagcaagaa gtaccagcgg
      601 aacttcgaga acaacaacct ggccgagaac caggaaaaca acaaaaacat catctacagc
      661 ggctccaaca tcttcaagaa catctacaac accgagatga tgtcaaacaa caacgccgtg
      721 gacgtgaaca tgatggacaa caaccctgcc gccagactgg aagaactgcg gaccatcatg
      781 aagaagaaca agattgacgt ctacatcctg atcaacagcg acgagcacaa cagcgagatc
      841 atcaacgaga aggacaagaa gatcgtcaag atcaccaact acgctggcgc cgacggaatc
      901 ctgatcgtga ccaaggacaa gcccatcctg tacgtgaacg ccctgtacga gctgcaggcc
      961 atgaacgagc tggaccagaa cctgttcacc ctgcggatca gccggatcga caaccgggac
     1021 gagatcttcg agacaatcag cagcctggaa ttcaacacaa tcgccttcga cggcaagaac
     1081 accgccgtgg tgttctacga gaagctgcgg aaggccctgc tgaacgccta ccccaagaaa
     1141 aagatcgtcg agaagatcat ctataacaac aatttcgacg acgtgaacaa gaaggacgac
     1201 gagaacgtcc tgaacttcct ggtgctggaa aagtccctgg tggaaatcaa ggactaccct
     1261 gtgaacaaca aggccctgta catccacgac cggaagtaca acggcgcctg tgccggcgag
     1321 aagattgaca agctgaagca gagcctgatg tacgacatca agaacgtgga caacctgctg
     1381 ctgagcgagc tggatgagat cgcctatctg ctgaacctgc ggggctacga ctaccagtac
     1441 agccctctgt tctacagcta cctgctgttc cagttcgaca gagaggaaca ggacttctcc
     1501 aagatcgtgt tcttcaccac cgtgaagaac ctgcccgccg atgtgaagaa tctgctcgag
     1561 atcaacaaag tgatcgtgaa agagtacgag gaaatcgtcc cctacctgcg ggacgtggtc
     1621 atccccagca tccccaagca caacgacgac aaccccgact ttaagaagta cgatatcagc
     1681 ctgagcccct acatcaacct gatgatctac aagctgttcg atcggaagaa cgtgctgctg
     1741 cagaacagcc ccgtggtcaa gatgaaggcc gtgaaaaacg acgtggaaat cgacaacatg
     1801 aagcaggccc acatcctgga cggcctggct ctgctgcagt tctttcattg gtgcgagcag
     1861 aagcgcaaga ccaaagagct gttcaacgag gccgagatga gcctgcggca caaggtggac
     1921 tacttcagaa gcaccaagaa gaacttcatc ttccccagct tcagcaccat cagcgccagc
     1981 ggccccaacg ccgccgtgat ccactacgag tgcaccgaca agaccaatgc cgccatcaag
     2041 cccgccatct acctgctcga ctctggcggc cagtacctgc acggcaccac cgacgtgacc
     2101 cggaccaccc actttggcga gcccaccgcc gaggaaaagc ggatctacac cctggtcctg
     2161 aagggccacc tgagactgcg gaaagtgatc ttcgccagct acaccaactc tgccgccctg
     2221 gactttatcg ccagagagaa tctgttcaac aacttcatgg actacaacca cggcaccggc
     2281 cacggcgtgg gcctgaccct gaatgtgcac gagggcggct gcagcatcgg ccctgtggga
     2341 ggcgcccctc tgaagaaaaa catggtgctg tccaacgagc ccggctacta catgaaggat
     2401 aagttcggcg tgcggatcga gaacatgcag tacgtgatca gcaaagagat caccgacacc
     2461 accgagtacc tgagcttcga cgacctgacc atgtaccctt acgagaagaa gctgctggac
     2521 ttcagcctgc tgaccaatca ggaaatcaaa gaactgaacg agtaccacac caccatccgg
     2581 aacaccctgc tgcccctcgt gaagcagtcc cctcaggaat acggcgagtc tgtcgagaag
     2641 tacctgatcg agatcacaga gcctatcgcc atccacaaca acggcgcgcc gtcgacctcc
     2701 atcacggcct ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga
     2761 gaggaaagcc tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc
     2821 tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc
     2881 aagttccagc tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag
     2941 tttgctggtt ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg
     3001 aacctggtgg tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg
     3061 ggacccacct cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc
     3121 tccaggcagg agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta
     3181 ctgagtgaag gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg
     3241 aattccggcg gcggctccgg aggcggtacc ggtggcggcg acctagcccc acagatgctt
     3301 cgagaactcc aggagactaa tgcggcgctg caagacgtga gagagctctt gcgacagcag
     3361 gtcaaggaga tcaccttcct gaagaatacg gtgatggaat gtgacgcctg cggtctagac
     3421 agaaacctgc ctccactagc acctctgggc ccacacccag aaacgctggt gaaagtaaaa
     3481 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3541 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3601 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3661 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3721 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3781 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3841 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3901 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3961 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4021 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4081 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4141 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4201 agacagatcg ctgagatagg tgcctcactg attaagcatt ggggccctga ctacaaagac
     4261 catgacggtg attataaaga tcatgacatc gattacaagg atgacgatga caagggccct
     4321 caccaccacc atcaccacta attaattaag gatcccccga cctcgacctc tggctaataa
     4381 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     4441 acatatggga gggcaaatca tttggtcgag atccctcgga gatctctagc tagaggatcg
     4501 atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct tcgcggggca
     4561 gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg ttccacatgt
     4621 gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat ccttataaat
     4681 ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt tgcagtctgt
     4741 ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt acaccaatgc
     4801 tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac caacgtcaat
     4861 cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag caatagtgtt
     4921 tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta gtagtatata
     4981 ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca tatgctatcg
     5041 aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca tgagctgtca
     5101 cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt agtcacaagg
     5161 gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc ctgcttcttc
     5221 attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag gtgtatgtga
     5281 ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac ggggggttca
     5341 gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg caataaatac
     5401 tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg ggtggggaca
     5461 agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa cacataatcc
     5521 tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca agacaggtga
     5581 accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg acagcagcgg
     5641 actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc caatggggcc
     5701 cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg gcacgcgtca
     5761 gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata acttggctga
     5821 ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact aatggcaccc
     5881 cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt gctgcgatct
     5941 ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt ggtcctcata
     6001 ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat atactaccca
     6061 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
     6121 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
     6181 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     6241 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctaata
     6301 gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac tacccaaata
     6361 tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     6421 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     6481 tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata
     6541 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta
     6601 tccgggtagc atatgctatc ctcatgataa gctgtcaaac atgagaattt tcttgaagac
     6661 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     6721 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6781 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6841 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     6901 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     6961 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7021 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7081 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     7141 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7201 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7261 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7321 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7381 agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7441 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7501 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7561 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7621 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7681 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     7741 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     7801 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7861 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     7921 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     7981 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     8041 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8101 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8161 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8221 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8281 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8341 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8401 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8461 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8521 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     8581 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     8641 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     8701 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     8761 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     8821 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     8881 atgattacgc caagctctag ctagaggtcg accaattctc atgtttgaca gcttatcatc
     8941 gcagatccgg gcaacgttgt tgccattgct gcaggcgcag aactggtagg tatggaagat
     9001 ctatacattg aatcaatatt ggcaattagc catattagtc attggttata tagcataaat
     9061 caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat
     9121 tggctcatgt ccaatatgac cgccatgttg acattgatta ttgactagtt attaatagta
     9181 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     9241 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     9301 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     9361 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat
     9421 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga
     9481 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     9541 ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     9601 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     9661 tcgtaataac cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     9721 tataagcaga gctcgtttag tgaaccgtca gatcctcact ctcttccgca tcgctgtctg
     9781 cgagggccag ctgttgggct cgcggttgag gacaaactct tcgcggtctt tccagtactc
     9841 ttggatcgga aacccgtcgg cctccgaacg gtactccgcc accgagggac ctgagcgagt
     9901 ccgcatcgac cggatcggaa aacct
//
