LOCUS       sequence-208768-        9000 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression of vsv-CenpB-1-166-dCEN-INCENP-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Liu D, Vader G, Vromans MJ, Lampson MA, Lens SM
  TITLE     Sensing chromosome bi-orientation by spatial separation of aurora B 
            kinase from kinetochore substrates.
  JOURNAL   Science. 2009 Mar 6;323(5919):1350-3. doi: 10.1126/science.1167000. 
            Epub 2009 Jan 15.
  PUBMED    19150808
REFERENCE   2  (bases 1 to 9000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1167000"; journalName: "Science"; date: "2009-03-6-
            6"; volume: "323"; issue: "5919"; pages: "1350-3"
COMMENT     Sequence Label: pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN- Incenp-GFP
FEATURES             Location/Qualifiers
     source          1..9000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1036..1068
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     regulatory      4237..4246
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4243..4245,4246..4248,4249..4962)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4243..4245,4246..4248,4249..4959)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4288..4309)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4549..4568)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4896..4917
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5008..5025)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5014..5238
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5284..5712
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5371..5390)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5581..5602
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5721..5741)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5726..6055
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5906..6041
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5968..5987
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6103..6150
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6169..6543
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6673..6794
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6710..6729)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6764..6783
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6843..6859)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6843..6859)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6856..6878)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6867..6883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6891..6897,6898..6915,6916..6921))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6936..6957
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7074..7091)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7245..7833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7325..7344)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8004..8795,8796..8864))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8627..8646
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8865..8969)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc tcgagctcaa gcttggtacc
     1021 ggatccgcca ccatgtacac cgacatcgag atgaaccggt tgggcaagga attcggcccc
     1081 aagaggcgac agctgacgtt ccgggagaag tcacggatca tccaggaggt ggaggagaat
     1141 ccggacctgc gcaagggcga gatcgcgcgg cgcttcaaca tcccgccgtc cacgctgagc
     1201 acgatcctga agaacaagcg cgccatcctg gcgtcggagc gcaagtacgg ggtggcctcc
     1261 acctgccgca agaccaacaa gctgtctccc tacgacaagc tcgagggctt gctcatcgcc
     1321 tggttccagc agatccgcgc cgccggcctg ccggtcaagg gcatcatcct caaggagaag
     1381 gcgctgcgca tagccgagga gctgggcatg gacgacttca ccgcctccaa cggctggctg
     1441 gaccgcttcc gccggcgcca cggcgtggtg tcctgcagcg gcgtggcccg cgcccgcgcg
     1501 cgaaacgctg ccccccgcac cccggcggcg cctgccagtc cggccgcggt gccctcggag
     1561 ggcgggagca ctgaattcag caaagagcca gagctgatgc ccaaaacacc ttctcagaag
     1621 aaccgacgga agaagagacg gatttcttat gttcaggatg aaaacagaga tcccatcagg
     1681 agaaggttat cccgcagaaa gtctcggagc agccagctga gctcccgacg cctccgcagc
     1741 aaggacagtg tagagaagct ggctacagtg gtcggggaga acggctccgt tctgcggcgt
     1801 gtgacccgtg ctgcggctgc agctgccgcg gctaccatgg cattggctgc accttcttca
     1861 cccacccctg agtctcccac gatgctgact aagaagcccg aggataacca cacccagtgc
     1921 cagctggtgc ctgtggtgga gatcggcatc agtgagcgcc agaatgctga gcagcatgtc
     1981 acccagctca tgtccaccga gcctctgccc cgcactctgt ccccgactcc agcttcagcc
     2041 acagctccaa cctcccaggg catcccgaca tcagatgagg aatcaacacc taagaagtcg
     2101 aaggccagga tactggagtc catcacagtg agctccctga tggctacacc ccaggacccc
     2161 aagggtcaag gggtcgggac ggggcggtct gcgtctaagc tcaggattgc gcaggtctcc
     2221 cctggcccac gggactcgcc agcctttcca gattctccat ggcgggagcg ggtgctggct
     2281 cccatcctgc cggataactt ctccacgccc acgggctctc gcacggactc tcaatcggtg
     2341 cggcacagcc cgatcgcccc gtcttccccg agtccccgag tcttagccca gaagtactct
     2401 ctggtggcca aacaggaaag tgttgtccgc agggcgagca gaaggcttgc caagaagact
     2461 gccgaagagc cagctgcctc tggccgcatc atctgtcaca gttacctgga gaggctcctg
     2521 aatgttgagg tgccccagaa agttggttct gagcagaagg aaccccccga ggaggctgag
     2581 cctgtggcgg cagctgagcc agaggtccct gagaacaacg gaaataactc gtggccccac
     2641 aatgacacag agatcgccaa cagcacaccc aacccgaagc ctgcagccag cagcccggaa
     2701 acaccctctg cagggcagca agaggccaag acggaccaag cagatggacc cagagagcca
     2761 ccgcagagtg ccaggaggaa gcgcagctac aagcaggccg tgagtgagct ggacgaggag
     2821 cagcacctgg aggatgagga gctgcagccc cccaggagca agaccccttc ctcaccctgc
     2881 ccagccagca aggtggtacg gcccctccgg acctttctgc acacagtgca gaggaaccag
     2941 atgctcacga ccccgacctc agccccacgc agcgtcatga agtcctttat taagcgcaac
     3001 actcccctgc gcatggaccc caagtgcagc ttcgtcgaga aggagcggca gcgcctggag
     3061 aatctgcggc ggaaggagga ggccgagcag ctgcgcaggc agaaggtgga ggaggacaag
     3121 cggcggcggc tggaggaggt gaagctgaag cgtgaggaac gcctccgcaa ggtgctgcag
     3181 gcccgcgagc gggtggagca gatgaaggag gagaagaaga agcagattga gcagaagttt
     3241 gctcagatcg acgagaagac tgagaaggcc aaggaggagc ggctggcaga ggagaaggcc
     3301 aagaaaaagg cggcggccaa gaagatggag gaggtggaag cacgcaggaa gcaggaagag
     3361 gatgcacgta ggctcaggtg gctgcagcag gaggaggaag agcggcggca ccaagagctg
     3421 ctgcagaaga agaaggaaga ggagcaggag cggctgcgga aggcggccga ggctaagcgg
     3481 ctggcagagc agcgggagca ggagcggcgg gagcaggagc ggcgcgagca ggagcggcgc
     3541 gagcaggagc ggcgggagca ggagcggcgc gagcaggagc gacagctggc agagcaggag
     3601 cgtcggcggg agcaggagcg gctccaggcc gagagggagc tgcaggagcg ggagaaggcc
     3661 ctgcggctgc agaaggagca gctgcagagg gaactggagg agaagaagaa gaaggaagag
     3721 cagcagcgtc tggctgagcg gcagctgcag gaggagcaag agaagaaagc caaggaggca
     3781 gcaggggcca gcaaggccct gaatgtgact gtggacgtgc agtctccagc ttgtacctca
     3841 tatcagatga ctccgcaagg gcacagggcc cctcccaaga tcaacccaga taactacggg
     3901 atggatctga atagcgacga ctccaccgat gatgaggccc atccccggaa gcccatcccc
     3961 acctgggccc gaggcacccc gctcagccag gctatcattc accagtacta ccacccaccg
     4021 aaccttctgg agctctttgg aaccattctc ccactggact tggaggatat cttcaagaag
     4081 agcaagcccc gctatcacaa gcgcaccagc tctgctgtct ggaactcacc gcccctgcag
     4141 ggcgccaggg tccccagcag cctggcctac agcctgaaga agcacaagct tcgaattctg
     4201 cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag
     4261 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     4321 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     4381 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     4441 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     4501 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     4561 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     4621 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     4681 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     4741 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     4801 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     4861 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     4921 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg ctcgagtcta
     4981 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     5041 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     5101 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     5161 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     5221 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     5281 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5341 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5401 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5461 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     5521 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5581 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5641 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5701 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     5761 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     5821 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5881 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5941 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     6001 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     6061 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     6121 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     6181 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     6241 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     6301 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     6361 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     6421 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     6481 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     6541 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     6601 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     6661 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6721 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6781 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     6841 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6901 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6961 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7021 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7081 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7141 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7201 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7261 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7321 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7381 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7441 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7501 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7561 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7621 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7681 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7741 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7801 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7861 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7921 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7981 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8041 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8101 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8161 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8221 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8281 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8341 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8401 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8461 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8521 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8581 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8641 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8701 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8761 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8821 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8881 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8941 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
