LOCUS       sequence-208776-        8214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of vsv-INCENP 8KQ .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   van der Horst A, Vromans MJ, Bouwman K, van der Waal MS, Hadders MA,
            Lens SM
  TITLE     Inter-domain Cooperation in INCENP Promotes Aurora B Relocation from
            Centromeres to Microtubules.
  JOURNAL   Cell Rep. 2015 Jul 21;12(3):380-7. doi: 
            10.1016/j.celrep.2015.06.038. Epub 2015 Jul 9.
  PUBMED    26166576
REFERENCE   2  (bases 1 to 8214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.06.038"; journalName: "Cell Rep"; date: 
            "2015-07-21- 21"; volume: "12"; issue: "3"; pages: "380-7"
COMMENT     Sequence Label: pCR3-vsv-Incenp 8KQ
FEATURES             Location/Qualifiers
     source          1..8214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(45..64)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        236..615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..819
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     770..790
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     864..883
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        864..882
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             911..943
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     promoter        complement(3767..3785)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3768..3785)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3805..3822)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3811..4035
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4081..4509
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4168..4187)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4378..4399
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4518..4538)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4523..4852
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4703..4838
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4765..4784
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4919..5713
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4973..4992)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5583..5602
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5887..6008
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5924..5943)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5978..5997
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6057..6073)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6057..6073)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6070..6092)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6081..6097
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6105..6111,6112..6129,6130..6135))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6150..6171
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6288..6305)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6459..7047)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6539..6558)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7218..8009,8010..8078))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7841..7860
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8079..8183)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggattcg ggagtactcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      421 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      781 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
      841 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agcttggtac
      901 cgccaccatg tacaccgaca tcgagatgaa ccggttgggc aaggggacga cggccccagg
      961 gcccattcac ctgctggagc tctgtgacca gaagctcatg gagtttctct gcaacatgga
     1021 taataaggac ttggtgtggc ttgaggagat ccaagaggag gccgagcgca tgttcaccag
     1081 agaattcagc aaagagccag agctgatgcc caaaacacct tctcagaaga accgacggaa
     1141 gaagagacgg atttcttatg ttcaggatga aaacagagat cccatcagga gaaggttatc
     1201 ccgcagaaag tctcggagca gccagctgag ctcccgacgc ctccgcagca aggacagtgt
     1261 agagaagctg gctacagtgg tcggggagaa cggctccgtt ctgcggcgtg tgacccgtgc
     1321 tgcggctgca gctgccgcgg ctaccatggc attggctgca ccttcttcac ccacccctga
     1381 gtctcccacg atgctgacta agaagcccga ggataaccac acccagtgcc agctggtgcc
     1441 tgtggtggag atcggcatca gtgagcgcca gaatgctgag cagcatgtca cccagctcat
     1501 gtccaccgag cctctgcccc gcactctgtc cccgactcca gcttcagcca cagctccaac
     1561 ctcccagggc atcccgacat cagatgagga atcaacacct aagaagtcga aggccaggat
     1621 actggagtcc atcacagtga gctccctgat ggctacaccc caggacccca agggtcaagg
     1681 ggtcgggacg gggcggtctg cgtctaagct caggattgcg caggtctccc ctggcccacg
     1741 ggactcgcca gcctttccag attctccatg gcgggagcgg gtgctggctc ccatcctgcc
     1801 ggataacttc tccacgccca cgggctctcg cacggactct caatcggtgc ggcacagccc
     1861 gatcgccccg tcttccccga gtccccgagt cttagcccag aagtactctc tggtggccaa
     1921 acaggaaagt gttgtccgca gggcgagcag aaggcttgcc aagaagactg ccgaagagcc
     1981 agctgcctct ggccgcatca tctgtcacag ttacctggag aggctcctga atgttgaggt
     2041 gccccagaaa gttggttctg agcagaagga accccccgag gaggctgagc ctgtggcggc
     2101 agctgagcca gaggtccctg agaacaacgg aaataactcg tggccccaca atgacacaga
     2161 gatcgccaac agcacaccca acccgaagcc tgcagccagc agcccggaaa caccctctgc
     2221 agggcagcaa gaggccaaga cggaccaagc agatggaccc agagagccac cgcagagtgc
     2281 caggaggaag cgcagctaca agcaggccgt gagtgagctg gacgaggagc agcacctgga
     2341 ggatgaggag ctgcagcccc ccaggagcaa gaccccttcc tcaccctgcc cagccagcaa
     2401 ggtggtacgg cccctccgga cctttctgca cacagtgcag aggaaccaga tgctcacgac
     2461 cccgacctca gccccacgca gcgtcatgaa gtcctttatt aagcgcaaca ctcccctgcg
     2521 catggacccc aagtgcagct tcgtcgagaa ggagcggcag cgcctggagc ttaagagatc
     2581 tgaaagcagc aatctgcggc ggcaggagga ggccgagcag ctgcgcaggc agaaggtgga
     2641 ggaggaccag cggcggcggc tggaggaggt gaagctgaag cgtgaggaac gcctccgcaa
     2701 ggtgctgcag gcccgcgagc gggtggagca gatgaaggag gagaagaaga agcagattga
     2761 gcagcagttt gctcagatcg acgagcagac tgagaaggcc caggaggagc ggctggcaga
     2821 ggagcaggcc aagaaaaagg cggcggccca gcagatggag gaggtggaag cacgcaggaa
     2881 gcaggaagag gatgcacgta ggctcaggtg gctgcagcag gaggaggaag agcggcggca
     2941 ccaagagctg ctgcagaaga agaaggaaga ggagcaggag cggctgcgga aggcggccga
     3001 ggctaagcgg ctggcagagc agcgggagca ggagcggcgg gagcaggagc ggcgcgagca
     3061 ggagcggcgc gagcaggagc ggcgggagca ggagcggcgc gagcaggagc gacagctggc
     3121 agagcaggag cgtcggcggg agcaggagcg gctccaggcc gagagggagc tgcaggagcg
     3181 ggagaaggcc ctgcggctgg gaagcagcgg atcccttaag gagcagctgc agagggaact
     3241 ggaggagaag aagaagaagg aagagcagca gcgtctggct gagcggcagc tgcaggagga
     3301 gcaagagaag aaagccaagg aggcagcagg ggccagcaag gccctgaatg tgactgtgga
     3361 cgtgcagtct ccagcttgta cctcatatca gatgactccg caagggcaca gggcccctcc
     3421 caagatcaac ccagataact acgggatgga tctgaatagc gacgactcca ccgatgatga
     3481 ggcccatccc cggaagccca tccccacctg ggcccgaggc accccgctca gccaggctat
     3541 cattcaccag tactaccacc caccgaacct tctggagctc tttggaacca ttctcccact
     3601 ggacttggag gatatcttca agaagagcaa gccccgctat cacaagcgca ccagctctgc
     3661 tgtctggaac tcaccgcccc tgcagggcgc cagggtcccc agcagcctgg cctacagcct
     3721 gaagaagcac aagctttgac tcgagcatgc atctagaggg ccctattcta tagtgtcacc
     3781 taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     3841 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     3901 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     3961 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     4021 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     4081 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4141 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4201 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4261 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     4321 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4381 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4441 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4501 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     4561 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     4621 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     4681 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4741 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     4801 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     4861 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     4921 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     4981 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     5041 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     5101 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     5161 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     5221 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     5281 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     5341 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     5401 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     5461 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     5521 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     5581 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     5641 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     5701 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     5761 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     5821 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     5881 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5941 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6001 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     6061 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6121 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6181 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6241 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6301 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6361 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6421 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6481 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6541 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6601 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6661 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6721 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6781 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6841 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6901 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6961 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7021 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7081 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7141 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7201 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7261 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7321 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7381 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7441 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7501 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7561 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7621 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7681 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7741 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7801 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7861 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7921 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7981 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8041 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8101 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8161 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
