LOCUS       sequence-208783-        8684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression of vsv-INCENP wt-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8684)
  AUTHORS   van der Horst A, Vromans MJ, Bouwman K, van der Waal MS, Hadders MA,
            Lens SM
  TITLE     Inter-domain Cooperation in INCENP Promotes Aurora B Relocation from
            Centromeres to Microtubules.
  JOURNAL   Cell Rep. 2015 Jul 21;12(3):380-7. doi: 
            10.1016/j.celrep.2015.06.038. Epub 2015 Jul 9.
  PUBMED    26166576
REFERENCE   2  (bases 1 to 8684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.06.038"; journalName: "Cell Rep"; date: 
            "2015-07-21- 21"; volume: "12"; issue: "3"; pages: "380-7"
COMMENT     Sequence Label: pcDNA5-FRT-TO-vsv-Incenp wt-GFP
FEATURES             Location/Qualifiers
     source          1..8684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1030..1062
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     regulatory      3865..3874
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3871..3873,3874..3876,3877..4590)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3871..3873,3874..3876,3877..4587)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3916..3937)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4177..4196)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4524..4545
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4636..4653)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4642..4866
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4999..5018)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5150..5197
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5205..6227
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5205..6227
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6357..6478
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6394..6413)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6448..6467
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6527..6543)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6527..6543)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6540..6562)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6551..6567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6575..6581,6582..6599,6600..6605))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6620..6641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6758..6775)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6929..7517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7009..7028)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7688..8479,8480..8548))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8311..8330
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8549..8653)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc tcgagctcaa gcttggtacc
     1021 gccaccatgt acaccgacat cgagatgaac cggttgggca aggggacgac ggccccaggg
     1081 cccattcacc tgctggagct ctgtgaccag aagctcatgg agtttctctg caacatggat
     1141 aataaggact tggtgtggct tgaggagatc caagaggagg ccgagcgcat gttcaccaga
     1201 gaattcagca aagagccaga gctgatgccc aaaacacctt ctcagaagaa ccgacggaag
     1261 aagagacgga tttcttatgt tcaggatgaa aacagagatc ccatcaggag aaggttatcc
     1321 cgcagaaagt ctcggagcag ccagctgagc tcccgacgcc tccgcagcaa ggacagtgta
     1381 gagaagctgg ctacagtggt cggggagaac ggctccgttc tgcggcgtgt gacccgtgct
     1441 gcggctgcag ctgccgcggc taccatggca ttggctgcac cttcttcacc cacccctgag
     1501 tctcccacga tgctgactaa gaagcccgag gataaccaca cccagtgcca gctggtgcct
     1561 gtggtggaga tcggcatcag tgagcgccag aatgctgagc agcatgtcac ccagctcatg
     1621 tccaccgagc ctctgccccg cactctgtcc ccgactccag cttcagccac agctccaacc
     1681 tcccagggca tcccgacatc agatgaggaa tcaacaccta agaagtcgaa ggccaggata
     1741 ctggagtcca tcacagtgag ctccctgatg gctacacccc aggaccccaa gggtcaaggg
     1801 gtcgggacgg ggcggtctgc gtctaagctc aggattgcgc aggtctcccc tggcccacgg
     1861 gactcgccag cctttccaga ttctccatgg cgggagcggg tgctggctcc catcctgccg
     1921 gataacttct ccacgcccac gggctctcgc acggactctc aatcggtgcg gcacagcccg
     1981 atcgccccgt cttccccgag tccccgagtc ttagcccaga agtactctct ggtggccaaa
     2041 caggaaagtg ttgtccgcag ggcgagcaga aggcttgcca agaagactgc cgaagagcca
     2101 gctgcctctg gccgcatcat ctgtcacagt tacctggaga ggctcctgaa tgttgaggtg
     2161 ccccagaaag ttggttctga gcagaaggaa ccccccgagg aggctgagcc tgtggcggca
     2221 gctgagccag aggtccctga gaacaacgga aataactcgt ggccccacaa tgacacagag
     2281 atcgccaaca gcacacccaa cccgaagcct gcagccagca gcccggaaac accctctgca
     2341 gggcagcaag aggccaagac ggaccaagca gatggaccca gagagccacc gcagagtgcc
     2401 aggaggaagc gcagctacaa gcaggccgtg agtgagctgg acgaggagca gcacctggag
     2461 gatgaggagc tgcagccccc caggagcaag accccttcct caccctgccc agccagcaag
     2521 gtggtacggc ccctccggac ctttctgcac acagtgcaga ggaaccagat gctcacgacc
     2581 ccgacctcag ccccacgcag cgtcatgaag tcctttatta agcgcaacac tcccctgcgc
     2641 atggacccca agtgcagctt cgtcgagaag gagcggcagc gcctggagaa tctgcggcgg
     2701 aaggaggagg ccgagcagct gcgcaggcag aaggtggagg aggacaagcg gcggcggctg
     2761 gaggaggtga agctgaagcg tgaggaacgc ctccgcaagg tgctgcaggc ccgcgagcgg
     2821 gtggagcaga tgaaggagga gaagaagaag cagattgagc agaagtttgc tcagatcgac
     2881 gagaagactg agaaggccaa ggaggagcgg ctggcagagg agaaggccaa gaaaaaggcg
     2941 gcggccaaga agatggagga ggtggaagca cgcaggaagc aggaagagga tgcacgtagg
     3001 ctcaggtggc tgcagcagga ggaggaagag cggcggcacc aagagctgct gcagaagaag
     3061 aaggaagagg agcaggagcg gctgcggaag gcggccgagg ctaagcggct ggcagagcag
     3121 cgggagcagg agcggcggga gcaggagcgg cgcgagcagg agcggcgcga gcaggagcgg
     3181 cgggagcagg agcggcgcga gcaggagcga cagctggcag agcaggagcg tcggcgggag
     3241 caggagcggc tccaggccga gagggagctg caggagcggg agaaggccct gcggctgcag
     3301 aaggagcagc tgcagaggga actggaggag aagaagaaga aggaagagca gcagcgtctg
     3361 gctgagcggc agctgcagga ggagcaagag aagaaagcca aggaggcagc aggggccagc
     3421 aaggccctga atgtgactgt ggacgtgcag tctccagctt gtacctcata tcagatgact
     3481 ccgcaagggc acagggcccc tcccaagatc aacccagata actacgggat ggatctgaat
     3541 agcgacgact ccaccgatga tgaggcccat ccccggaagc ccatccccac ctgggcccga
     3601 ggcaccccgc tcagccaggc tatcattcac cagtactacc acccaccgaa ccttctggag
     3661 ctctttggaa ccattctccc actggacttg gaggatatct tcaagaagag caagccccgc
     3721 tatcacaagc gcaccagctc tgctgtctgg aactcaccgc ccctgcaggg cgccagggtc
     3781 cccagcagcc tggcctacag cctgaagaag cacaagcttc gaattctgca gtcgacggta
     3841 ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
     3901 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     3961 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     4021 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     4081 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     4141 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     4201 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     4261 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     4321 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     4381 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     4441 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     4501 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     4561 actctcggca tggacgagct gtacaagtaa agcggccgct cgagtctaga gggcccgttt
     4621 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     4681 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     4741 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     4801 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     4861 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     4921 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4981 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     5041 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     5101 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtacctag aagttcctat
     5161 tccgaagttc ctattctcta gaaagtatag gaacttcctt ggccaaaaag cctgaactca
     5221 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     5281 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     5341 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     5401 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     5461 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     5521 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     5581 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     5641 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     5701 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     5761 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     5821 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     5881 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     5941 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     6001 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     6061 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     6121 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     6181 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac gtactacgag
     6241 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     6301 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     6361 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6421 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6481 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6541 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6601 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6661 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6721 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6781 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6841 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6901 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6961 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7021 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7081 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7141 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7201 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7261 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7321 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7381 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7441 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7501 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7561 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7621 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7681 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7741 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7801 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7861 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7921 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7981 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8041 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8101 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8161 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8221 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8281 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8341 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8401 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8461 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8521 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8581 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8641 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
