LOCUS       sequence-208784-       11063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-10a-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11063)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 11063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-10a-5p
FEATURES             Location/Qualifiers
     source          1..11063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    1196..1220
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1231..1240
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1237..1929
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGH"
     protein_bind    complement(1952..1976)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    2269..2324
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    2708..2733
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    3405..3425
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        3474..4652
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3705..4643
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    4669..4693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4698..4707
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4704..4706,4707..4709,4710..5423)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(4749..4770)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(5425..5449)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5604..5659
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6020..6036)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6020..6036)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6033..6055)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6044..6060
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6068..6074,6075..6092,6093..6098))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6113..6134
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6348..6367)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6359..7168
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6426..6445)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      complement(7420..8008)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7500..7519)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8179..8970,8971..9039))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8802..8821
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9040..9111)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    9128..9165
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             9196..9795
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9196..9215)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9692..9712
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9961..10016
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     protein_bind    10995..11015
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgtgg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag aagggcgaat tcgacccaag
     1201 tttgtacaaa aaagcaggct ggatccaggt gccaccatgg tgagcgagct gattaaggag
     1261 aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca
     1321 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggcggtcgag
     1381 ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaaa
     1441 accttcatca accacaccca gggcatcccc gacttcttta agcagtcctt ccccgagggc
     1501 ttcacatggg agagagtcac cacatacgaa gatgggggcg tgctgaccgc tacccaggac
     1561 accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttccca
     1621 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggcctccac cgagacactg
     1681 taccccgctg acggcggcct ggaaggcaga gccgacatgg ccctgaagct cgtgggcggg
     1741 ggccacctga tctgcaacct taagaccaca tacagatcca agaaacccgc taagaacctc
     1801 aagatgcccg gcgtctacta tgtggacagg agactggaaa gaatcaagga ggccgacaaa
     1861 gagacatacg tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa
     1921 ctggggcaca aacttaattg agaattctac cacccagctt tcttgtacaa agtggtacgt
     1981 ctcaagacca gaggtctctg cttgtctcag tcacaaattc ggatctacag ggtacacaaa
     2041 ttcggatcta cagggtacac aaattcggat ctacagggta cacaaattcg gatctacagg
     2101 gtacaaccag aagagacctg tgcttcactc ctcaggtgca ggctgcctat cagaaggtgg
     2161 tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca
     2221 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
     2281 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     2341 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
     2401 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
     2461 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
     2521 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
     2581 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
     2641 tatggagatc ccgagcggtg cgtttttatg cttgtagtat tgtataatgt ttttaagatc
     2701 caagcttctg acctcttctc ttcctcccac agggcctcga gacaattgca tcgatggtac
     2761 cagtgaaagt ctgcaatgaa ttcgagttgg cgcgcctgtc attctaaatc tctctttcag
     2821 cctaaagctt tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc
     2881 gttcagagga aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc
     2941 cggctcgggg atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg
     3001 ccccctagcg ggggagggac gtaattacat ccctgggggc tttgggaggg ggctgtcccc
     3061 gtgagctccc aggcgcgcct gtcattctaa atctctcttt cagcctaaag ctttttcccc
     3121 gtatcccccc aggtgtctgc aggctcaaag agcagcgaga agcgttcaga ggaaagcgat
     3181 cccgtgccac cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg
     3241 gggagcgccg gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggggagg
     3301 gacgtaatta catccctggg ggctttgggg gggggctgtc cccgtgagct cccaggcgcg
     3361 ccaactcggt accgtatcga tgtcgacgtt aacgctagtg atatcaactt tgtatagaaa
     3421 agttggctcc gaattcgccc ttgacagcag agatccagtt tggttaatta attggctccg
     3481 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg tggggagggg
     3541 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     3601 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     3661 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
     3721 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
     3781 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
     3841 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
     3901 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
     3961 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
     4021 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
     4081 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
     4141 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
     4201 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
     4261 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
     4321 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     4381 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     4441 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     4501 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     4561 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     4621 aaagtttttt tcttccattt caggtgtcgt gagaagggcg aattcgaccc aagtttgtac
     4681 aaaaaagcag gctgaccgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     4741 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgaggggcg
     4801 agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca
     4861 agctgcccgt gccctggccc accctcgtga ccaccctgag ccacggcgtg cagtgcttcg
     4921 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     4981 acgtccagga gcgcaccatc ttcttcaagg acgacggcac ctacaagacc cgcgccgagg
     5041 tgaagttcga gggcgacacc ctagtgaacc gcatcgagct gaagggcgtc gacttcaagg
     5101 aggacggcaa catcctgggg cacaagctgg agtacaactt caacagccac aacatctata
     5161 tcatggccgt caagcagaag aacggcatca aggtgaactt caagatccgc cacaacgtgg
     5221 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     5281 ccgtgctgct gcccgacagc cactacctga gcacccagtc cgtgctgagc aaagacccca
     5341 acgagaagcg cgatcacatg gtcctgctgg agttccgcac cgccgccggg atcactctcg
     5401 gcatggacga gctgtacaag taatacccag ctttcttgta caaagtggta cgcgtgaatt
     5461 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     5521 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
     5581 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     5641 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
     5701 gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa
     5761 aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
     5821 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
     5881 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
     5941 ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc
     6001 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6061 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6121 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6181 gccagcggat cgacagtact aagcttggtc taatacccaa tctctcgtct tatccagatg
     6241 ttttatacgc ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     6301 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     6361 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
     6421 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
     6481 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     6541 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     6601 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     6661 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     6721 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     6781 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     6841 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     6901 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     6961 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     7021 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     7081 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     7141 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca
     7201 gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag
     7261 ttacgcgtcg ttccactgag cgtcagaggt atcactagta ttacagaggt aagttataac
     7321 agtcgcctaa cccaattgag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7381 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7441 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7501 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7561 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7621 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7681 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7741 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7801 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7861 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7921 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7981 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8041 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8101 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8161 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8221 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8281 gggcttacca tctggcccca gtgctgcaat gataccgcga gaaccacgct caccggctcc
     8341 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8401 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8461 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8521 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8581 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8641 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8701 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8761 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8821 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8881 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8941 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9001 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9061 ttgaagcatt tatcaaggtt attgtctcat gagcggatac atatttgaat gtaccgaatt
     9121 tctcgagggc ttgtcgacga cggcggtctc cgtcgtcagg atcatgaatt cgaccgcctg
     9181 tctcaaggtg ccaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     9241 gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     9301 accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     9361 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     9421 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     9481 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     9541 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     9601 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     9661 gtgcccgcct tcctggagac atccgcgccc cgcaacctcc ccttctacga gcggctcggc
     9721 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     9781 aagcccggtg cctgagcttt gagactacgc gtgaattcac tcctcaggtg caggctgcct
     9841 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt
     9901 ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct
     9961 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
    10021 gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt
    10081 tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc
    10141 agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag
    10201 gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt
    10261 ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg
    10321 tccctcttct cttatggaga tccctcgacc tgcagcccaa gcttgaattc tctagaatta
    10381 ccctgttatc cctatgcttg gtttaccgag ctcttattgg ttttcaaact tcattgactg
    10441 tgcccaacgc gcctgggagc tcacggggac agcccccccc caaagccccc agggatgtaa
    10501 ttacgtccct cccccgctag ggggcagcag cgagccgccc ggggctccgc tccggtccgg
    10561 cgctcccccc gcatccccga gccggcagcg tgcggggaca gcccgggcac ggggaaggtg
    10621 gcacgggatc gctttcctct gaacgcttct cgctgctctt tgagcctgca gacacctggg
    10681 gggatacggg gaaaaagcta ggctgaaaga ccggttctta gaatgacacg cgcctgggag
    10741 ctcacgggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta
    10801 gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg
    10861 agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc
    10921 tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaagct
    10981 aacttctgag acgacaactt tgtatagaaa agttggctcc gaattcgccc ttgacagcag
    11041 agatccagtt tggttaatta att
//
