LOCUS       sequence-208790-       11059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-let-7a-2-3p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11059)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 11059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-let-7a-2-3p
FEATURES             Location/Qualifiers
     source          1..11059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    1196..1220
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1231..1240
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1237..1929
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGH"
     protein_bind    complement(1952..1976)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    2265..2320
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    2704..2729
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    3401..3421
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        3470..4648
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3701..4639
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    4665..4689
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4694..4703
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4700..4702,4703..4705,4706..5419)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(4745..4766)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(5421..5445)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5600..5655
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6016..6032)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6016..6032)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6029..6051)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6040..6056
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6064..6070,6071..6088,6089..6094))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6109..6130
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6344..6363)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6355..7164
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6422..6441)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      complement(7416..8004)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7496..7515)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8175..8966,8967..9035))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8798..8817
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9036..9107)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    9124..9161
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             9192..9791
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9192..9211)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9688..9708
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9957..10012
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     protein_bind    10991..11011
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgtgg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag aagggcgaat tcgacccaag
     1201 tttgtacaaa aaagcaggct ggatccaggt gccaccatgg tgagcgagct gattaaggag
     1261 aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca
     1321 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggcggtcgag
     1381 ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaaa
     1441 accttcatca accacaccca gggcatcccc gacttcttta agcagtcctt ccccgagggc
     1501 ttcacatggg agagagtcac cacatacgaa gatgggggcg tgctgaccgc tacccaggac
     1561 accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttccca
     1621 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggcctccac cgagacactg
     1681 taccccgctg acggcggcct ggaaggcaga gccgacatgg ccctgaagct cgtgggcggg
     1741 ggccacctga tctgcaacct taagaccaca tacagatcca agaaacccgc taagaacctc
     1801 aagatgcccg gcgtctacta tgtggacagg agactggaaa gaatcaagga ggccgacaaa
     1861 gagacatacg tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa
     1921 ctggggcaca aacttaattg agaattctac cacccagctt tcttgtacaa agtggtacgt
     1981 ctcaagacca gaggtctctg cttccttacg tggaaagcta ggaggctgta cagggaaagc
     2041 taggaggctg tacagggaaa gctaggaggc tgtacaggga aagctaggag gctgtacagc
     2101 aaccagaaga gacctgtgct tcactcctca ggtgcaggct gcctatcaga aggtggtggc
     2161 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
     2221 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     2281 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     2341 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
     2401 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
     2461 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     2521 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     2581 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     2641 gagatcccga gcggtgcgtt tttatgcttg tagtattgta taatgttttt aagatccaag
     2701 cttctgacct cttctcttcc tcccacaggg cctcgagaca attgcatcga tggtaccagt
     2761 gaaagtctgc aatgaattcg agttggcgcg cctgtcattc taaatctctc tttcagccta
     2821 aagctttttc cccgtatccc cccaggtgtc tgcaggctca aagagcagcg agaagcgttc
     2881 agaggaaagc gatcccgtgc caccttcccc gtgcccgggc tgtccccgca cgctgccggc
     2941 tcggggatgc ggggggagcg ccggaccgga gcggagcccc gggcggctcg ctgctgcccc
     3001 ctagcggggg agggacgtaa ttacatccct gggggctttg ggagggggct gtccccgtga
     3061 gctcccaggc gcgcctgtca ttctaaatct ctctttcagc ctaaagcttt ttccccgtat
     3121 ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg
     3181 tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga
     3241 gcgccggacc ggagcggagc cccgggcggc tcgctgctgc cccctagcgg gggagggacg
     3301 taattacatc cctgggggct ttgggggggg gctgtccccg tgagctccca ggcgcgccaa
     3361 ctcggtaccg tatcgatgtc gacgttaacg ctagtgatat caactttgta tagaaaagtt
     3421 ggctccgaat tcgcccttga cagcagagat ccagtttggt taattaattg gctccggtgc
     3481 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttgtggg gaggggtcgg
     3541 caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     3601 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     3661 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc
     3721 ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg
     3781 gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag
     3841 gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg
     3901 gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc
     3961 tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct
     4021 tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga
     4081 cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc
     4141 gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc
     4201 gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc
     4261 ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc
     4321 gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt
     4381 cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag
     4441 cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca
     4501 cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga
     4561 atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag
     4621 tttttttctt ccatttcagg tgtcgtgaga agggcgaatt cgacccaagt ttgtacaaaa
     4681 aagcaggctg accgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     4741 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtga ggggcgaggg
     4801 cgagggcgat gccaccaacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     4861 gcccgtgccc tggcccaccc tcgtgaccac cctgagccac ggcgtgcagt gcttcgcccg
     4921 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     4981 ccaggagcgc accatcttct tcaaggacga cggcacctac aagacccgcg ccgaggtgaa
     5041 gttcgagggc gacaccctag tgaaccgcat cgagctgaag ggcgtcgact tcaaggagga
     5101 cggcaacatc ctggggcaca agctggagta caacttcaac agccacaaca tctatatcat
     5161 ggccgtcaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acgtggagga
     5221 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     5281 gctgctgccc gacagccact acctgagcac ccagtccgtg ctgagcaaag accccaacga
     5341 gaagcgcgat cacatggtcc tgctggagtt ccgcaccgcc gccgggatca ctctcggcat
     5401 ggacgagctg tacaagtaat acccagcttt cttgtacaaa gtggtacgcg tgaattcact
     5461 cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac
     5521 aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt
     5581 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat
     5641 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat
     5701 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt
     5761 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat
     5821 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct
     5881 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga
     5941 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag
     6001 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6061 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6121 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6181 gcggatcgac agtactaagc ttggtctaat acccaatctc tcgtcttatc cagatgtttt
     6241 atacgcctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     6301 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     6361 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     6421 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     6481 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     6541 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     6601 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     6661 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     6721 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     6781 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     6841 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     6901 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     6961 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     7021 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     7081 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     7141 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     7201 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     7261 gcgtcgttcc actgagcgtc agaggtatca ctagtattac agaggtaagt tataacagtc
     7321 gcctaaccca attgagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7381 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7441 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7501 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7561 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7621 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7681 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7741 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7801 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7861 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7921 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7981 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8041 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8101 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8161 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8221 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8281 ttaccatctg gccccagtgc tgcaatgata ccgcgagaac cacgctcacc ggctccagat
     8341 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8401 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8461 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8521 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8581 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8641 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8701 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8761 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8821 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8881 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8941 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9001 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9061 agcatttatc aaggttattg tctcatgagc ggatacatat ttgaatgtac cgaatttctc
     9121 gagggcttgt cgacgacggc ggtctccgtc gtcaggatca tgaattcgac cgcctgtctc
     9181 aaggtgccac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     9241 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     9301 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     9361 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     9421 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     9481 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     9541 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     9601 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     9661 ccgccttcct ggagacatcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     9721 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     9781 ccggtgcctg agctttgaga ctacgcgtga attcactcct caggtgcagg ctgcctatca
     9841 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
     9901 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     9961 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
    10021 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
    10081 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
    10141 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
    10201 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
    10261 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
    10321 tcttctctta tggagatccc tcgacctgca gcccaagctt gaattctcta gaattaccct
    10381 gttatcccta tgcttggttt accgagctct tattggtttt caaacttcat tgactgtgcc
    10441 caacgcgcct gggagctcac ggggacagcc cccccccaaa gcccccaggg atgtaattac
    10501 gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct
    10561 ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac
    10621 gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga
    10681 tacggggaaa aagctaggct gaaagaccgg ttcttagaat gacacgcgcc tgggagctca
    10741 cggggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg
    10801 gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca tccccgagcc
    10861 ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa
    10921 cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa aaagctaact
    10981 tctgagacga caactttgta tagaaaagtt ggctccgaat tcgcccttga cagcagagat
    11041 ccagtttggt taattaatt
//
