LOCUS       sequence-208792-        7977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression vector for fusion of Gal4 DNA binding domain to 
            estrogen receptor alpha hormone binding domain to VP16 
            transcriptional activation domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Louvion JF, Havaux-Copf B, Picard D
  TITLE     Fusion of GAL4-VP16 to a steroid-binding domain provides a tool for 
            gratuitous induction of galactose-responsive genes in yeast.
  JOURNAL   Gene 1993 Sep 6;131(1):129-34.
  PUBMED    8370533
REFERENCE   2  (bases 1 to 7977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1993-09-6"; volume: "131(1)"; pages: "129-34"
COMMENT     Sequence Label: pHCA/GAL4(1-93).ER.VP16
FEATURES             Location/Qualifiers
     source          1..7977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        339..1043
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     primer_bind     complement(2599..2615)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2599..2615)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2647..2666)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2648..2666)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2673..2690)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2673..2689)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2682..2704)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2830..3285
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2917..2936)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3127..3148
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3545..4207)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(4208..4395)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(4542..4561)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4661..4683
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4721..4739)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    4787..5290
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        5320..5424
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5425..5493,5494..6285)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5643..5662)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6456..7044
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6945..6964
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7198..7215
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7332..7353
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7368..7373,7374..7391,7392..7398)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7406..7422
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7411..7433
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7430..7446
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7430..7446
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7467..7485
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     7513..7529
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7514..7530
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acttgcgaga cgcgagtttg ccggtggtgc gaacaataga gcgaccatga ccttgaaggt
       61 gagacgcgca taaccgctag agtactttga agaggaaaca gcaatagggt tgctaccagt
      121 ataaatagac aggtacatac aacactggaa atggttgtct gtttgagtac gctttcaatt
      181 catttgggtg tgcactttat tatgttacaa tatggaaggg aactttacac ttctcctatg
      241 cacatatatt aattaaagtc caatgctagt agagaagggg ggtaacaccc ctccgcgctc
      301 ttttccgatt tttttctaaa ccgtggaata tttcggatat ccttttgttg tttccgggtg
      361 tacaatatgg acttcctctt ttctggcaac caaacccata catcgggatt cctataatac
      421 cttcgttggt ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc
      481 agacaagaca taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt
      541 acataacgaa ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac
      601 tacccttttt ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt
      661 tttttttctt ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaatg
      721 atggaagaca ctaaaggaaa aaattaacga caaagacagc accaacagat gtcgttgttc
      781 cagagctgat gaggggtatc tcgaagcaca cgaaactttt tccttccttc attcacgcac
      841 actactctct aatgagcaac ggtatacggc cttccttcca gttacttgaa tttgaaataa
      901 aaaaaagttt gctgtcttgc tatcaagtat aaatagacct gcaattatta atcttttgtt
      961 tcctcgtcat tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag
     1021 ctataccaag catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac
     1081 tgtcttctat cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag
     1141 aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa
     1201 ccaaaaggtc tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac
     1261 tggaacagct atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg
     1321 attctttaca ggatataaaa gcattgttgg gtacccctgc agctgcgtcg actctagagg
     1381 atccatctgc tggagacatg agagctgcca acctttggcc aagcccgctc atgatcaaac
     1441 gctctaagaa gaacagcctg gccttgtccc tgacggccga ccagatggtc agtgccttgt
     1501 tggatgctga gccccccata ctctattccg agtatgatcc taccagaccc ttcagtgaag
     1561 cttcgatgat gggcttactg accaacctgg cagacaggga gctggttcac atgatcaact
     1621 gggcgaagag ggtgccaggc tttgtggatt tgaccctcca tgatcaggtc caccttctag
     1681 aatgtgcctg gctagagatc ctgatgattg gtctcgtctg gcgctccatg gagcacccag
     1741 tgaagctact gtttgctcct aacttgctct tggacaggaa ccagggaaaa tgtgtagagg
     1801 gcatggtgga gatcttcgac atgctgctgg ctacatcatc tcggttccgc atgatgaatc
     1861 tgcagggaga ggagtttgtg tgcctcaaat ctattatttt gcttaattct ggagtgtaca
     1921 catttctgtc cagcaccctg aagtctctgg aagagaagga ccatatccac cgagtcctgg
     1981 acaagatcac agacactttg atccacctga tggccaaggc aggcctgacc ctgcagcagc
     2041 agcaccagcg gctggcccag ctcctcctca tcctctccca catcaggcac atgagtaaca
     2101 aaggcatgga gcatctgtac agcatgaagt gcaagaacgt ggtgcccctc tatgacctgc
     2161 tgctggagat gctggacgcc caccgcctac atgcgcccac tagccgtgga ggggcatccg
     2221 tggaggagac ggaccaaagc cacttggcca ctgcgggctc tacttcatcg gagctccact
     2281 tagacggcga ggacgtggcg atggcgcatg ccgacgcgct agacgatttc gatctggaca
     2341 tgttggggga cggggattcc ccgggtccgg gatttacccc ccacgactcc gccccctacg
     2401 gcgctctgga tatggccgac ttcgagtttg agcagatgtt taccgatgcc cttggaattg
     2461 acgagtacgg tgggtagggg gcgcgaccgg acccgcatcc cccgtctggg ttttcccctc
     2521 ccgtccccgg ttcgtatcca caataaacac gagcacatac attacaaaaa cctgcggttg
     2581 tcgtctgatt atttggtgga tccactagtt ctagagcggc cgccaccgcg gtggagctcc
     2641 aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac
     2701 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     2761 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     2821 ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     2881 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     2941 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     3001 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     3061 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     3121 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     3181 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     3241 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt
     3301 tctccttacg catctgtgcg gtatttcaca ccgcatagat ccgtcgagtt caagagaaaa
     3361 aaaaagaaaa agcaaaaaga aaaaaggaaa gcgcgcctcg ttcagaatga cacgtataga
     3421 atgatgcatt accttgtcat cttcagtatc atactgttcg tatacatact tactgacatt
     3481 cataggtata catatataca catgtatata tatcgtatgc tgcagcttta aataatcggt
     3541 gtcactacat aagaacacct ttggtggagg gaacatcgtt ggtaccattg ggcgaggtgg
     3601 cttctcttat ggcaaccgca agagccttga acgcactctc actacggtga tgatcattct
     3661 tgcctcgcag acaatcaacg tggagggtaa ttctgctagc ctctgcaaag ctttcaagaa
     3721 aatgcgggat catctcgcaa gagagatctc ctactttctc cctttgcaaa ccaagttcga
     3781 caactgcgta cggcctgttc gaaagatcta ccaccgctct ggaaagtgcc tcatccaaag
     3841 gcgcaaatcc tgatccaaac ctttttactc cacgcacggc ccctagggcc tctttaaaag
     3901 cttgaccgag agcaatcccg cagtcttcag tggtgtgatg gtcgtctatg tgtaagtcac
     3961 caatgcactc aacgattagc gaccagccgg aatgcttggc cagagcatgt atcatatggt
     4021 ccagaaaccc tatacctgtg tggacgttaa tcacttgcga ttgtgtggcc tgttctgcta
     4081 ctgcttctgc ctctttttct gggaagatcg agtgctctat cgctagggga ccacccttta
     4141 aagagatcgc aatctgaatc ttggtttcat ttgtaatacg ctttactagg gctttctgct
     4201 ctgtcatctt tgccttcgtt tatcttgcct gctcattttt tagtatattc ttcgaagaaa
     4261 tcacattact ttatataatg tataattcat tatgtgataa tgccaatcgc taagaaaaaa
     4321 aaagagtcat ccgctaggtg gaaaaaaaaa aatgaaaatc attaccgagg cataaaaaaa
     4381 tatagagtgt actagaggag gccaagagta atagaaaaag aaaattgcgg gaaaggactg
     4441 tgttatgact tccctgacta atgccgtgtt caaacgatac ctggcagtga ctcctagcgc
     4501 tcaccaagct cttaaaacgg gaatttatgg tgcactctca gtacaatctg ctctgatgcc
     4561 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     4621 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     4681 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     4741 ttataggtta atgtcatgat aataatggtt tcttagggtc cttttcatca cgtgctataa
     4801 aaataattat aatttaaatt ttttaatata aatatataaa ttaaaaatag aaagtaaaaa
     4861 aagaaattaa agaaaaaata gtttttgttt tccgaagatg taaaagactc tagggggatc
     4921 gccaacaaat actacctttt atcttgctct tcctgctctc aggtattaat gccgaattgt
     4981 ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg tgattttaca ttttacttat
     5041 cgttaatcga atgtatatct atttaatctg cttttcttgt ctaataaata tatatgtaaa
     5101 gtacgctttt tgttgaaatt ttttaaacct ttgtttattt ttttttcttc attccgtaac
     5161 tcttctacct tctttattta ctttctaaaa tccaaataca aaacataaaa ataaataaac
     5221 acagagtaaa ttcccaaatt attccatcat taaaagatac gaggcgcgtg taagttacag
     5281 gcaagcgatc cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5341 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5401 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5461 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5521 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5581 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5641 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5701 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5761 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5821 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5881 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5941 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     6001 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6061 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6121 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6181 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6241 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6301 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6361 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6421 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6481 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6541 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6601 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6661 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6721 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6781 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6841 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6901 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6961 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7021 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7081 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     7141 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     7201 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     7261 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     7321 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     7381 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     7441 tatgaccatg attacgccaa gctcgaaatt aaccctcact aaagggaaca aaagctggta
     7501 ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc ctgcagcccg
     7561 ggggatccgg gatcgaagaa atgatggtaa atgaaatagg aaatcaagga gcatgaaggc
     7621 aaaagacaaa tataagggtc gaacgaaaaa taaagtgaaa agtgttgata tgatgtattt
     7681 ggctttgcgg cgccgaaaaa acgagtttac gcaattgcac aatcatgctg actctgtggc
     7741 ggacccgcgc tcttgccggc ccggcgataa cgctgggcgt gaggctgtgc ccggcggagt
     7801 tttttgcgcc tgcattttcc aaggtttacc ctgcgctaag gggcgagatt ggagaagcaa
     7861 taagaatgcc ggttggggtt gcgatgatga cgaccacgac aactggtgtc attatttaag
     7921 ttgccgaaag aacctgagtg catttgcaac atgagtatac tagaagaatg agccaag
//
