LOCUS       sequence-208806-       13430 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13430)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU27.1-2.2
FEATURES             Location/Qualifiers
     source          1..13430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      2268..2277
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7865..7885)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7865..7883)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(8222..8810)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8302..8321)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8981..9772,9773..9841))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9604..9623
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9842..9946)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9973..11315
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10936..10983)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11356..11374
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11646..12050
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             12063..13157
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(13405..13421)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13405..13421)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatgcata
      541 accgagcttc cgcattaacc tcagatacag ccgccataat gactatcacg aacagttcgc
      601 aggcgccgat tgcaattgta ggcatgtcct gtcgccttcc aggccaggtt cgtacgcttg
      661 atgacttctg gactctcata tctcgcggta gggatgcttg gggccctatc ccttctgatc
      721 gcatgtccag tgcggcctac taccacccag atccccagcg aaagggctgt ttcaacccca
      781 aagggggtta ttaccttcag gaggatttct ctcagtttga cgctccgttc ttccacataa
      841 cgcaacaaga agccattgcc atggatcccc aacagcgact actactcgaa tgtgcctatg
      901 aagccctgga aaacgccggt tttccccaac aagccacggc aggctcgcgc atgggggtct
      961 tcacgagcct ttgttcatca gactaccgtc gtggtgcttt tcgcgacctg gactccgttc
     1021 ccatttttga cgccacgggg aatcaggaat ctatccaagc gggtcgcctt tcccatttct
     1081 tcaacctccg tggcccatgt atggcgatcg atacggcctg ttcgtccagt ctctatgccc
     1141 tgcatctggc ggtgcagagt attcgttctg gcgatgcgga ttcagccctg gttggtggag
     1201 ctagactgcg tttgcagcca gatgagatgg tttcaatgtc gatgatgggt ttatacaacg
     1261 agcaaggaaa aacctttgca tttgatgatc gtgccgtttc gggctttgcc tgcggtgagg
     1321 gagtgggctg tcttgtcctc aagccattag accaggccct ggaagacaac gatccgatct
     1381 attcagttat taggaacaca ggggcaaatc atgatggtcg aactgtcggc atgacgaacc
     1441 ccaacggaga cgctcaagaa gaactcatgc gagaagtcta cgcccgcgcc gacctctccc
     1501 cgattgatac cggattcgtt gaggcgcacg gcaccggaac caaggttggc gatcccatcg
     1561 aagtcggcgc catccatcgg gtgtttgggg atggccggac caagcgggca ccgctatacc
     1621 taggctccgt caaatccaac ttcggacatc tcgagtgcgc cagcggtatt gcgtccgtga
     1681 tcaaagcgtc tctgatgctc caccgtggct tcgttcttcc caatgccaac tttgagcgcg
     1741 ccaatcccgc catccctctt gaccaatgga atatcaaggt gccgaccaat ctgcgcccct
     1801 ggccccaggg caagaagtac atcagtatca acaacttcag cttcggtggc tccaactgcc
     1861 acgtcgtact ggaacggcct ccccgttcgc gcggccagcc tcccaacccc caacacgatg
     1921 cgccccagct ctttgtactc agcggctatg atgacgaggc tgcgaaacgc attgccagga
     1981 acctcacgtt ctacttggaa caacatcccg gtgtcttcga acggcgcctg gtgcacgata
     2041 tcgcatatac acttggcgag cgacgctctc actttccctg gcgcgttgct gtcaccgctt
     2101 catctaccgc ggaacttgtc gaaaccctga atgggccagc gagtgtccct cagcgcgccc
     2161 ggctggaggc tggctctgca cctgttattg cctttgcgta cactggacag ggcgcacaat
     2221 ggccccgcat gggattggaa cttagcgaca cccaccctat ttttgctgcc accatggagg
     2281 aagcagcacg cgcactggag gatctgcgag cggatttctc cctgttcgaa gagctgaagc
     2341 gtgaggcagc tgactctcgc gtcagtcagc cccagatctc tcaagtagtt tgcactgccg
     2401 tgcagcttgc cctgacggat cttttggctt cctggggcgt acaaccgcaa atggttgtcg
     2461 gccactccag cggagagatt ggggcggcct atgcggtggg tgctatttcc gtgagcgagg
     2521 ccattgccct ggcatatcat cggggtcgac tcgctgccca ggttcgaatc cgattcccaa
     2581 cgttgaatgg ttgcatgatg gccgtcggag agagtgccga tgaggtgcgc aaaaccatca
     2641 agcaacttca gctaggagag gacatcactg tcgcttgtga gaattcacct cggtccacca
     2701 cggcgtctgg agatgccact gccatggacc ttctcgcaaa ggagctggaa gcgcgacaga
     2761 tctttcatcg gaagcttatg gtcgacgttg cctatcactc tccccacatg cagctgatcg
     2821 ccgatgagta taccagcgcc atccagcagg tcgcaccatc cactaagagg gagaatgtcc
     2881 agtactactc ctcgctgtta ggtgaaaaga tcgagcaacc cggggctctg ggagcatcgt
     2941 attgggttga taacctgacg cacccggtcc gattctctac cgcgctgggc cagctgtgcg
     3001 ctgagtccag acccgatatc atcattgaga tcggtccgca ttccgcgttg cagggcccga
     3061 tcaagcagat actgcagggc atccagaata cgcacactca atactttgcc tcccttgtcc
     3121 gcaaggagca cgccactaag gctgctctac agctcgcggg ccggttgttc atggccggcc
     3181 agcagctggc tttccgtgag gttaaccaaa ctcacctgga tcagtcagtc ccaacagtgt
     3241 tgccgtccct gacgccgtat ccctggtccc atcgctccta ctggcatgag agccgcatcg
     3301 ccgtcaacca ccgactgcca cgcttccctc gtcacgacct gcttggacga ttggaagcct
     3361 ctacccgcga ggatgaacag cccgtctggc gcaattccgt gtctctggac agcgtgccat
     3421 ggctccgcgg tcatcgtatg caatccatga ccaccttccc aatggccggc tacatcagca
     3481 tggccgtcga ggcggcatct caacgggctc agctcagagg cctctccccg tcccagattg
     3541 ccgggtaccg gctccgtgag ttccatgctt cctcggcgtt caccctggag gagaacgtcg
     3601 agtacgaaac ctggctctct ctgagctcgt tcacggaggg cacccgctcc tactccagcg
     3661 aatgggacga atttcgtatt gcctcgtggg cggccggtcg gggttggcgt gagcattgcc
     3721 gtggtctagt gggcatccgg cgccaggagt cgtccactac taaccctgtt agtcaacgcc
     3781 ggccgtttca tgctagcctg gagcggcgtg aggacgctaa acagggtgac caccgtcctg
     3841 tatcactaaa gactttctac tcagacctag agggccgcgg agccggatac agcgacgcgt
     3901 tcacgctcga ttcagacagc ggggtgaccg ttggaagagc aggcgtcggg aacagctggt
     3961 atgcgcgcgg aaaggttaca gttcccaaca ccgcgcaaag catgccctca ggccatgaga
     4021 ccccgtttat tgtgtctcct gcgttcctgg atctattttt ccagttgtcc ttccccgttt
     4081 tgcgggcgga ctctgccaac attccgtgcc tcttcatgcc gacttctatc cgggagatca
     4141 atatcgcagc caatttccca tcgcaaccgg gagaaacggc ggaggcccta gtcaatggac
     4201 tgccggcaga ttcctcccgt cctcggccat tggactatac ccttgatgca tggtctccgt
     4261 ctgcgtccac agagcaaccc gtcctcacta tccaagggtt ccgaatgacc cctgtcaatg
     4321 gcgatatgct gattgacacc accccgtatc cgtggtgcta cactgtacag tgggagccac
     4381 tgccttctgc aggtgaagca aatggcacgg cccaaagtgt cccagagagt aatggacatg
     4441 gcgagacgaa tggcaatggc attgtgcatg cgaacggcaa tgatgttgtt catggcaatg
     4501 gcaatcacaa tgcatctaat ggaagctgcc atgtgtcatc agaagacgag tcaaccgtca
     4561 tcatcatcac tgagaggccc agctctgatc cacttatcgc cactctcctg acagctattg
     4621 atgccttcac ggggtacaga accacggtcg cccccctctc tcgcgtccag atctcgtcca
     4681 aggatcgata catctgcctg gctgagctcg atactccact tctgcagtca ctgaccccgg
     4741 aatccttcaa acatgtccaa gagctcctcg tcacgtgctc ctactgcctc tgggttacct
     4801 ccggcgcata catggctgtc gatcatcccg agctaaacat tgttcaaggt ctgctgcgtt
     4861 ctgtgcgttc agaaaccgga aactcagctg tcagccttga cctggatcct agttctcgcc
     4921 aagacatcgc aggtcgggct catctaatcc ttgatgcctt caaagcgtcc accaacgctg
     4981 tccagacagc cgccgatgac gaggaggaag cgcctctatc cgattatgag ttcaccgaag
     5041 tcggtggacg cctgatggtc cctcgcctag tagagcattc agaattgaac aataccgtgt
     5101 ttcaagagac acacccatct cttccgtaca ttcaggactt cgaacaagga acggatcgcc
     5161 gactcaagat tgccgtcggc accctgggcg ccctggactc actctactgg accgatgatc
     5221 cggcccagcc gctcggcgcc gacgagatcg agatcaaggt ggcctgcaca ggcgccaact
     5281 tcaaggatgt ggtaattgcc atgggccagg tcgccagtcc ttacttgggc atcgagtgca
     5341 gtggcacggt ggcgcgcgtc ggagccaagg tcagttccct caagcccgga gaccgcgtat
     5401 gcgccatgtc ccacggcgcg tatagcacct acgcgcggtg tccggcaacc agcgcggctg
     5461 tgatcccaga ctcaatggac tttgagacgg cagcttccat ccccgttacc tacagcacgg
     5521 cttactatgg acttgtcgag ttggctcaca tggaagatgg ggaaagtgtc ctcatccacg
     5581 ccgcggcggg aggcgtcggt caagccgcca ttcagctggc tcagctaatg ggagccgaga
     5641 tctacgccac ggttggtagt gaggagaaga agcagatgct gatcgatcga tactgtatcc
     5701 cggcgagtcg cattttctat agtcgcaaca ccgagtttgg cccttgtgtc cgcgaagcca
     5761 cgggcggacg cggcgtggac gtggttctga actcactggc gggcgatttc ctgcgcgaga
     5821 cgtgggattg tctggctccg tttggacggt tcattgagat tggaaaacgg gatattactt
     5881 ccaatactcg gctggagatg ggcaagttcg agtacaactg cacgttttcc tccttggact
     5941 tgacgcttgt tgcggaccag aagggcaagg tcctcaaccg tgtatttacc gctgtcatga
     6001 ggatgttcgc acagggaaga ctgaccccgg tatatccagt gacgacggta ggtatcgcgg
     6061 aggtggaatc ggtgctgcgc aagttgcaga gtggcaagac gacgggcaag gtcgtagtgg
     6121 atcatcggat taatgggaag atcaaggcca cacatcccct accgtcgagt gatctacttt
     6181 caccggatgc tacgtacatt gttgtcggtg gcaccggcgg tattggccaa tcgatcactc
     6241 gcagaatggt tcagcgcggt gctaggcata ttgtcctgct ctctcgcagt ggcgccgtca
     6301 cggaagccgc ccagaaaatc atcagcgacg gccaagccgt ggatgcatcg atccagatca
     6361 aaaaatgcga tgtgtccgaa gtttctcagg tcaagtcact gattgcagaa ctacagaaag
     6421 agctaccacc agtccggggt gttattcacg cggccatggt cctcaaggac gttttcctgg
     6481 agcaaatgac attcccagac tgggaagccg tcctccgtcc caaggtcgcg gcagcctgga
     6541 acctccacta cgcgcttctc caccaaccac tggacttctt cgtcatgctt tcctccgtgt
     6601 ctggcatcgt cggcagtcgc ggccaagccg cctacgcagc gggaaactgc ttcctggatg
     6661 cattggcacg ctaccgccgc caaaagggcc tatccgccgt atccattgac ttgacggcag
     6721 taggcgacat cggggtattc tcggaagcag cagacaagaa aaaggcagaa gtgctgagga
     6781 acctggcgat gagcaacaca atgcgagaag ttgaagtact agctctgatc gagctcgcga
     6841 tgaagggcca gcaagtccca gaacagtgca tcaccggtgt gcattggcct tcctccgccg
     6901 caactccgta ctatgcttct gatgcccgct tcacccatct tgtcgagacc gcgaagcagg
     6961 aggaaggcgc tgatgccacg gcccatgccg gagcaactaa atctctctct atcacccagc
     7021 aagtccgccg cgccgcgaac ctgcaggaag ccctcgatgt tacggcggtc ggtctacgtg
     7081 ataagttggg tgacattctc atgcttcccc gagaagtggt ggaagcgcat acgcagtcga
     7141 ctcccattgc gtcgtttggg ctggattcat tgaatgcaat tgagctgagg aattggattg
     7201 gcaaggagct gaaggggcat ttgcaggttt tggagttgtt gtcggcggga actttggggt
     7261 cgttggcgtt ggtggtgttg aagaagtcga gtttggaggg ggcgtggaag ggagaaatcc
     7321 caaactaagg agattgataa gacttttcta gttgcatatc ttttatattt aaatcttatc
     7381 tattagttaa ttttttgtaa tttatcctta tatatagtct ggttattcta aaatatcatt
     7441 tcagtatcta aaaattcccc tcttttttca gttatatctt aacaggcgac agtccaaatg
     7501 ttgatttatc ccagtccgat tcatcagggt tgtgaagcat tttgtcaatg gtcgaaatca
     7561 catcagtaat agtgcctctt acttgcctca tagaatttct ttctcttaac gtcaccgttt
     7621 ggtcttttat agtttcgaaa tctatggtga taccaaatgg tgttcccaat tcatcgttac
     7681 gggcgtattt tttaccaatt gaagtattgg aatcgtcaat tttaaagtat atctctcttt
     7741 tacgtaaagc ctgcgagatc ctcttaagta tagcggggaa gccatcgtta ttcgatattg
     7801 tcgtaacaaa tactttgatc ggcgctatgc ggccgccacc gcggtggagc tccagctttt
     7861 gttcccttta gtgagggtta attgcgcgct tggcgagact caaacaagat atattttgcc
     7921 cgccttacag acgaaactag ttggaggtta tggagcatac tatcacgtgg gcggccactg
     7981 gtgagttact acaccccagg ggcaacgttg atgctcctaa aaaactctgg ctggacgcaa
     8041 gccgtaacac ccgtgtcact tcataatcgt ttgcaattca gggcttgatc tacacattac
     8101 cctgttatcc ctattaatta atacggttat ccacagaatc aggggataac gcaggaaaga
     8161 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8221 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8281 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8341 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8401 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8461 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8521 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8581 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8641 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     8701 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8761 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8821 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8881 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8941 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9001 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9061 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9121 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9181 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9241 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9301 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9361 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9421 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9481 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9541 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9601 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9661 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9721 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9781 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9841 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9901 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     9961 aagtgccacc tgaacgaagc atctgtgctt cattttgtag aacaaaaatg caacgcgaga
    10021 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg
    10081 aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg
    10141 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca
    10201 acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc tacaaaaatg
    10261 catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt ctcctttgtg
    10321 cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa ggttagaaga
    10381 aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt
    10441 tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc cccgattata
    10501 ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg ttgatgattc
    10561 ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata ctacgtatag
    10621 gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct tactacaatt
    10681 tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc gagtttagat
    10741 gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc acagagatat
    10801 atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat attttagtag
    10861 ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt
    10921 tttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggaac
    10981 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag
    11041 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag
    11101 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat
    11161 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt
    11221 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca
    11281 tccttcaatg ctatcatttc ctttgatatt ggatcatact aagaaaccat tattatcatg
    11341 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
    11401 gacggtgaaa acctcggcgc gccattaccc tgttatccct aatatacaac gcaaagaagc
    11461 tggaaaattg gcgagggaat cctgtttctg tctatccaag aatgggcatg aggtggcaac
    11521 cgtcgtgcta gcgtacaggg tgcactttgt aaccatttgg gacaccggac actcgctgtt
    11581 ttcgaaatta ccctttaagc gcgggtattg aaccaggctt atgcccaaga tcgtagcaag
    11641 catttaactg tgggaatact caggtatcgt aagatgcaag agttcgaatc tcttagcaac
    11701 cattattttt ttcctcaaca taacgagaac acacaggggc gctatcgcac agaatcaaat
    11761 tcgatgactg gaaatttttt gttaatttca gaggtcgcct gacgcatata cctttttcaa
    11821 ctgaaaaatt gggagaaaaa ggaaaggtga gaggccggaa ccggcttttc atatagaata
    11881 gagaagcgtt catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa
    11941 ggacctattg ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac
    12001 cttttacatt tcagcaatat atatatatat ttcaaggata taccattcta atgtctgccc
    12061 ctatgtctgc ccctaagaag atcgtcgttt tgccaggtga ccacgttggt caagaaatca
    12121 cagccgaagc cattaaggtt cttaaagcta tttctgatgt tcgttccaat gtcaagttcg
    12181 atttcgaaaa tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtc ccacttccag
    12241 atgaggcgct ggaagcctcc aagaaggttg atgccgtttt gttaggtgct gtggctggtc
    12301 ctaaatgggg taccggtagt gttagacctg aacaaggttt actaaaaatc cgtaaagaac
    12361 ttcaattgta cgccaactta agaccatgta actttgcatc cgactctctt ttagacttat
    12421 ctccaatcaa gccacaattt gctaaaggta ctgacttcgt tgttgtcaga gaattagtgg
    12481 gaggtattta ctttggtaag agaaaggaag acgatggtga tggtgtcgct tgggatagtg
    12541 aacaatacac cgttccagaa gtgcaaagaa tcacaagaat ggccgctttc atggccctac
    12601 aacatgagcc accattgcct atttggtcct tggataaagc taatcttttg gcctcttcaa
    12661 gattatggag aaaaactgtg gaggaaacca tcaagaacga attccctaca ttgaaggttc
    12721 aacatcaatt gattgattct gccgccatga tcctagttaa gaacccaacc cacctaaatg
    12781 gtattataat caccagcaac atgtttggtg atatcatctc cgatgaagcc tccgttatcc
    12841 caggttcctt gggtttgttg ccatctgcgt ccttggcctc tttgccagac aagaacaccg
    12901 catttggttt gtacgaacca tgccacggtt ctgctccaga tttgccaaag aataaggttg
    12961 accctatcgc cactatcttg tctgctgcaa tgatgttgaa attgtcattg aacttgcctg
    13021 aagaaggtaa ggccattgaa gatgcagtta aaaaggtttt ggatgcaggt atcagaactg
    13081 gtgatttagg tggttccaac agtaccaccg aagtcggtga tgctgtcgcc gaagaagtta
    13141 agaaaatcct tgcttaaaaa gattctcttt ttttatgata tttgtacata aactttataa
    13201 atgaaattca taatagaaac gacacgaaat tacaaaatgg aatatgttca tagggtagac
    13261 gaaactatat acgcaatcta catacattta tcaagaagga gaaaaaggag gatagtaaag
    13321 gaatacaggt aagcaaattg atactaatgg ctcaacgaaa tgacggtatc gataagcttg
    13381 atatcgaatt cctgcagccc gggggatcca ctagttctag agttaattaa
//
