LOCUS       sequence-208807-       13373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13373)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU28.1-2.2
FEATURES             Location/Qualifiers
     source          1..13373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(7808..7828)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7808..7826)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(8165..8753)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8245..8264)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8924..9715,9716..9784))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9547..9566
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9785..9889)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9916..11258
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10879..10926)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11299..11317
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11589..11993
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             12006..13100
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(13348..13364)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13348..13364)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatggcgc
      541 ccattgccat cactccggac aacagctcca ccggcagccc gtcaggggag agcgtcaacg
      601 gctacttcca tgtcagcacc gacaaccgcc aggccaatgg gcataccaac gggcacacca
      661 atggctacgc cagcagacac ctagatggac acccgaacgg gcattcaaac ggagccggta
      721 atggtgtttt caatggcgtc aatggcgtca acggcgtcca cgggccctcg ggaggtttct
      781 ctacttccca taagccgatg cccatcgcca ttgtgggaat ggcttgtcgc atgccgggca
      841 atgtctctac tcctgccgag ttctgggagc tctgcacacg ggctcgctcg gggtggaccg
      901 agatccccaa ggaacgattc gacagctccc ggttttacca cccaaacccc ggcaaggggg
      961 gcgccctgaa tccagtcggc gggaacttcc tcaatgttga tctcgccgcc ttcgacgccc
     1021 ctttcttcgg gctcacagag aaggaagcca tatctatgga tccccagcag cgccttctcc
     1081 tggagtgtac gttcgaggcc ctcgagaacg cgggaatccc caagcacacc atcgtcggga
     1141 aggacgtcgg cgttttcatc ggcggctcgt tctccgagta cgagtcacac ctgttccggg
     1201 attccgatac gattcccatg catcaggcaa ccggctgcgc atttgcgatg cagtccaacc
     1261 gtatctcgca tttctttgac ctgagaggcc cgagtttcac gtccgacaca gcctgttcgt
     1321 ccagcatggt cgctgtgcat ctggcttgcc agagtctgcg ggccggcgag attagctgtg
     1381 ccctggtcgg cgcctgccat ctcaacatgc taccggagtt ttggatctcc ttttcgactt
     1441 gcaggtacgt ccccgttcca gagcccggca catccgcaat cagggacaat gacaccatca
     1501 gggcagtcat tgtgggaacc ggcttgaacc aggacggaaa gacgcccggc atcaccatgc
     1561 caaacggagc cgcccaagag gccctgatga gacaggtgta caagaacgcg ggaatcgacc
     1621 cacgcgactg cggcttcgtt gaagctcacg gaacgggtac ccgggtgggg gatcccatcg
     1681 aggccaccgc catccacaac gtgctgggcc agaacaggac agcacgcgat cccctgtgga
     1741 tagggtctgt caagtccaac attggccatc tcgaaggcgc ttcagggatt gctggcatca
     1801 taaaagctgc cctgatgctc gagcgcggtt tcatcctccc caactacgac ttcaagcaac
     1861 caaaccccaa gattccttgg aaggagtgga atctcaaggt cccgattgcc cagcggccat
     1921 ggccgcgcgg taagaaatac atcagcgtca ataactttgg gtttggcggg acaaacggtc
     1981 acgtcgtgct cgaggctccg ccattccgag gaccaaaggg ttctgccgcc ggcggtgacg
     2041 acacatctgg aaaggcagga caaggacgga agctctacgt cttcacggcc aacgacaaga
     2101 cggcgctctc gcaggttatg aagaacatcg tcatttatct tgaacagcgc ccagaaatct
     2161 tccaaaagga cctgacagga catctagcct acaccttggg gcagcgccga tctctcctgc
     2221 agtggagagc ggccattccg gccgtcaatt ctttcgagct cattgaagcg attaacggcg
     2281 aaaagtacac tcccggaaag gagactgggc cgctaaggat cggcttcatt ctcaccgggc
     2341 agggcgcgca gtggcatgcc atgggacgag agctctatga gcagtatccc accttcagca
     2401 gcagcctcga gctggccgac aggtgtctgg ctgagaacgg tgctgactgg tccctgatcg
     2461 aggaactggg aagagacgca aaaaccagca agattaacga ggcccatatc agtcaaccgt
     2521 cgtgtactgc catccaactg gcgctgattg acttgctgcg gtcctggggc attcgtccaa
     2581 ccgccgttgc tggtcattca agcggcgaaa tcgcggccgc ctacgccgcg gagatcatcg
     2641 acttcgactc cgccatgcgg attgcgtacc atcgcggcag gcttattccc atcctcaaac
     2701 gatctcatcc ggagctcaga ggccgcatga tggccgttgg cggcagcaaa gagcagttcc
     2761 tgcccattct cgacggcctc aaggagaagg aagcccgcat cgcctgctac aacagcccct
     2821 cgagcctgac catctcgggc gatgagccgg ccattgccga gctcgagaag atctgcgagg
     2881 agaagcagct ctttaacagg cgtctcgtgg tggacgtggc ataccattcc caccacatga
     2941 acctggtggc gaaggagtac cgagcgtcgc tggccgacct gcggcccccc gttcccaccg
     3001 acatccggtt ccactcatcc ctttacgggc gcctggtcga cggtacggag ctgcagccgg
     3061 actactgggt ggagaacctg acctgcgcag tgcgcttttc ggaagcgttg cagagcatgc
     3121 tggaacccgc cggcgagcac aagcacggcg tcaacatgct cgtcgagctt ggtccgcatt
     3181 ccgggctcca ggggccgatc aagcaaatcc tgaaggagat cggcggcagc gcccttaaga
     3241 tcccgtacgc gtccgctctc gtacggaagc gtgatgcggt agagacggcg ctggaactcg
     3301 ctgcggatct gttcaccagg ggcgccagcc tcaacttcgg ggcaatcaac ttccccaagc
     3361 cgaccaagcc gcccgtgctg ctgaccgacc tgccgcggta cccctggaat tactcgtcca
     3421 ggtattggca ggagtctcgc atgacaagag cacacaagca cgctcctggc ggccggagcg
     3481 acattctagg caccctggcc aactactcca acgacttgga gcccacctgg cgcaacattg
     3541 tccggctgga tgacctcccc tggctgcggc agcacaagat ccagtccttg actgtctttc
     3601 ccatggccgg gttcattgcc atggccctcg aggcagcggc tcaaagagca tccgcgaggg
     3661 gctgcccgtt cgacagtttc gagctgaagg acgtctcgat taccaagcct ctcgtcattc
     3721 cagacaagga catcgaggtc acgctcaccc tcagaccacg aaaggagagc ggtccctcct
     3781 cccctggcag ttgggacgag ttccgcatct gctcctggtc gagcgaacag ggctggaccg
     3841 agcactgcgt tggcctcatt gcggctctgg aagcggatgt caacgaggtc gacagtgcca
     3901 ggcgcgctcg agacgcggag gctcgtctca agaccatcct gtctcggact agccagccgg
     3961 aagcggtgcc tgtgactcca caagccatgt atgacagcct ctcgaagctg ggcgtggcct
     4021 acggggctct tttccaggga gtaaccagct gctacgcatc cgactcccac gcggtgggga
     4081 accttgtggt gccagacgtc gcaaaggaga tgcccagcgg ttatctaacc gatgcggttc
     4141 tccagccggc gttcctcgag tctctcattg agatgtattg gcccattgtc ggagctggtc
     4201 gacgtcccgt cggcaccatc tatctgccct cgtctgtcga ccgggttact gtctctctcg
     4261 gcgtccagcg gtttaccaag gaccctggca gcgcggtccg cgccgtttgc gaaggcgaga
     4321 tcgccgctgg ttcgagcccc aagccagcca aggtcaacgt ggccgccgtc acgggcgacg
     4381 acaagcttga gacgctcatc tccctggatg ggctcaccat ttcccccatc ctcgacggcg
     4441 agacgcaggc ggactccgcc gcagcgaggg aactctgcta taagttggag tgggatccga
     4501 tcctcgaaac cgacgacgag agcgtccaag accttccaac ggacgcccag gtcgtcatca
     4561 tccacgagga ctccagtttc caaagtctcg tggcaatggg cctggcaaac tctctggagc
     4621 aggccactgg gaggctggct gactttggca ctctggaaac cgtcgacgct gccggcaaga
     4681 tctgcgtctt tctgagcgag ctgcaccagc cgttccttgc gacactcaca cccgcccagt
     4741 tcgacggcct gcagaggctg ttgacgtccg tcgagggcgt actgtgggtc gtgcgcggag
     4801 cgtacgacaa ctcggcaaac ccagatgcca acatggtgac cggcttgagc cgaaccatcc
     4861 gatcagagac tgcgctgaag tttgctaccc tcgatctgga cgccgagtca ccgctctcgg
     4921 agcttgagac ggccgacgcg atcatgaagg tcttcaagtt cgtctttcac tcgggttcct
     4981 ccatgtctgg agaactggag ttcatggaga gaggcgggcg tttctacacg cctcgaattg
     5041 ttcacgatcc cgagatggac gagtacgtcc acaagcagac aaacccatcc gctctcgagc
     5101 ctacgctgtt tggggaggac caccgagctt tgaagatggc actgtcagcg ccgggcgcgt
     5161 tggagacgct ccacttcgtt gacgacccgg ctgctgagca gccgctggag gccgacgagg
     5221 ttgagattga ggttaaggcc atcggactga acttccggga cggtatggct gccaaaggcc
     5281 aaatccctct gggcagttgc ggaatcgaag caagcggagt cgtcaccgcg gtgggaagca
     5341 aagtcacggc ctaccgggct ggcgatcgtg tcgcggcgct taccacaggt gcgtttgcga
     5401 cgcgaacgag gacaaaggcg gccttcgcct tcaagatctc ggcggacctc acattcgagg
     5461 ctgctgccag cctgccgctc gcgtatgcga cagcatacta cagcttggta gagcttgggc
     5521 ggctcggtga aggcgagact gtcctcatcc actccgcggc cggcgctgtt ggacaagcag
     5581 ccgtctgcat cgcacaaatg atcggtgctg aggtctttgt gactgttgga agtgcggaga
     5641 agaaagagct cctgttgaag gagttcggac tcccggacga gcgtgtcttc tacagccgca
     5701 acagctcctt tgcagacgca atttgccgag atacgcaggg gcatggcgtc gacgtcatcc
     5761 tcaacaccct caccggggac gggctccgcg agtcttggag atgtctgaac aagttcgggc
     5821 gcttcattga gcttggccgg ggagagaatg tctctaaggc gcggctggaa gtcgacagaa
     5881 tcgacagcaa cgccagcttc atctgccttg acatgctggc cctgatctct gagcggccaa
     5941 agaccatcaa gcggctactt gcggacgttg cgcagctctt gaagtacggc aagatcaggc
     6001 ccgcagttcc catcacaaga ttccccatct cggacgtcga aacggcgttg aagacgcagc
     6061 aaactgggaa gtcaatgggc aagctcgtgg tggttcccag tgcgactgac gtcgtcaagg
     6121 ctgtacaggc tgctcgttct aggaaggcga agcccctcct ccgctcggat gccacctata
     6181 tcctggtcgg cggcactggt ggcctcggcc gcagttttgc taggtggatg gcggcgaagg
     6241 gagcaaggaa catcgtgctg gtttcgagaa gcggctccgt aacgggcagg gtaaaggagc
     6301 tcgtcgacga acttggcgca attggcacca acgtcgttgt tcgccgctgc aacgttgtca
     6361 acaagagcga agtggacgag cttattagct ccatcctcgg cgacctgccc cctatccgcg
     6421 gtgtggtgca tggcaccatg gtactcaggg atgtgctttt cgaaaagatg gcgtgggagg
     6481 attacacgca ggtcatcgag ggcaaggtgc aaggcggatg gaacttccac cacgcactcg
     6541 cagactcgcc gctcgacttc ttcgtggcta tctcctcggc cgcaggcgcc gtcggcaacc
     6601 gcgggcaggc cgcctacagc gcggcgaact gcttcctcaa cgcgctggtg cagcaccgtc
     6661 tcgcccgcgg cctgcccgcg gcctcgctcg acctgaccgc cgtttccgac tcgggctacc
     6721 tggccgagga cctcgagaag gcggccgagg tggcgaggaa cctcggcagc gacagtatct
     6781 gcgagagcga ggtgctcgcg ctgctccagg ccgccatcga cggcacgatg gcctcgacct
     6841 gcaacagcca cgccatcacc ggcatgcgca tcaccgcgac catgcggccg ttctggaccg
     6901 aggatgccaa gttcaaggcg atgcgcatcg cggccgagga ggcggcggcc aaggacgctt
     6961 cgctcaacgc cgtggtgtcc ttcaatgctg ccctcaagca ggcaaccacg cgcgccgagg
     7021 cggaggatgt catctgccgc ggcctggtcg acaagatctc ctcggtcctg atgatggaag
     7081 ccgaggagtt ggacatcacc aggtcgctgt cccattaccc gctggattcg ctggtggcga
     7141 ttgagatccg caactttatc acgcgcgagt tcgaggcgaa tctgcaggtt ttggagctgc
     7201 tgtcgagcgg gtcgatccag acgcttgcga agggggtctg cgcgaagagc aagcttgtta
     7261 ctttctctta gggagattga taagactttt ctagttgcat atcttttata tttaaatctt
     7321 atctattagt taattttttg taatttatcc ttatatatag tctggttatt ctaaaatatc
     7381 atttcagtat ctaaaaattc ccctcttttt tcagttatat cttaacaggc gacagtccaa
     7441 atgttgattt atcccagtcc gattcatcag ggttgtgaag cattttgtca atggtcgaaa
     7501 tcacatcagt aatagtgcct cttacttgcc tcatagaatt tctttctctt aacgtcaccg
     7561 tttggtcttt tatagtttcg aaatctatgg tgataccaaa tggtgttccc aattcatcgt
     7621 tacgggcgta ttttttacca attgaagtat tggaatcgtc aattttaaag tatatctctc
     7681 ttttacgtaa agcctgcgag atcctcttaa gtatagcggg gaagccatcg ttattcgata
     7741 ttgtcgtaac aaatactttg atcggcgcta tgcggccgcc accgcggtgg agctccagct
     7801 tttgttccct ttagtgaggg ttaattgcgc gcttggcgag actcaaacaa gatatatttt
     7861 gcccgcctta cagacgaaac tagttggagg ttatggagca tactatcacg tgggcggcca
     7921 ctggtgagtt actacacccc aggggcaacg ttgatgctcc taaaaaactc tggctggacg
     7981 caagccgtaa cacccgtgtc acttcataat cgtttgcaat tcagggcttg atctacacat
     8041 taccctgtta tccctattaa ttaatacggt tatccacaga atcaggggat aacgcaggaa
     8101 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8161 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8221 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8281 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8341 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8401 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8461 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8521 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8581 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     8641 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8701 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8761 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8821 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8881 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8941 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9001 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9061 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9121 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9181 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9241 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9301 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9361 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9421 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9481 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9541 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9601 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9661 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9721 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9781 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9841 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9901 gaaaagtgcc acctgaacga agcatctgtg cttcattttg tagaacaaaa atgcaacgcg
     9961 agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac
    10021 gcgaaagcgc tattttacca acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca
    10081 acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat
    10141 gcaacgcgag agcgctattt taccaacaaa gaatctatac ttcttttttg ttctacaaaa
    10201 atgcatcccg agagcgctat ttttctaaca aagcatctta gattactttt tttctccttt
    10261 gtgcgctcta taatgcagtc tcttgataac tttttgcact gtaggtccgt taaggttaga
    10321 agaaggctac tttggtgtct attttctctt ccataaaaaa agcctgactc cacttcccgc
    10381 gtttactgat tactagcgaa gctgcgggtg cattttttca agataaaggc atccccgatt
    10441 atattctata ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga
    10501 ttcttcattg gtcagaaaat tatgaacggt ttcttctatt ttgtctctat atactacgta
    10561 taggaaatgt ttacattttc gtattgtttt cgattcactc tatgaatagt tcttactaca
    10621 atttttttgt ctaaagagta atactagaga taaacataaa aaatgtagag gtcgagttta
    10681 gatgcaagtt caaggagcga aaggtggatg ggtaggttat atagggatat agcacagaga
    10741 tatatagcaa agagatactt ttgagcaatg tttgtggaag cggtattcgc aatattttag
    10801 tagctcgtta cagtccggtg cgtttttggt tttttgaaag tgcgtcttca gagcgctttt
    10861 ggttttcaaa agcgctctga agttcctata ctttctagag aataggaact tcggaatagg
    10921 aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata
    10981 cagctcactg ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag
    11041 aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag
    11101 gatgaaaggt agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg
    11161 cttccttcag cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc
    11221 tcatccttca atgctatcat ttcctttgat attggatcat actaagaaac cattattatc
    11281 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
    11341 gatgacggtg aaaacctcgg cgcgccatta ccctgttatc cctaatatac aacgcaaaga
    11401 agctggaaaa ttggcgaggg aatcctgttt ctgtctatcc aagaatgggc atgaggtggc
    11461 aaccgtcgtg ctagcgtaca gggtgcactt tgtaaccatt tgggacaccg gacactcgct
    11521 gttttcgaaa ttacccttta agcgcgggta ttgaaccagg cttatgccca agatcgtagc
    11581 aagcatttaa ctgtgggaat actcaggtat cgtaagatgc aagagttcga atctcttagc
    11641 aaccattatt tttttcctca acataacgag aacacacagg ggcgctatcg cacagaatca
    11701 aattcgatga ctggaaattt tttgttaatt tcagaggtcg cctgacgcat ataccttttt
    11761 caactgaaaa attgggagaa aaaggaaagg tgagaggccg gaaccggctt ttcatataga
    11821 atagagaagc gttcatgact aaatgcttgc atcacaatac ttgaagttga caatattatt
    11881 taaggaccta ttgttttttc caataggtgg ttagcaatcg tcttactttc taacttttct
    11941 taccttttac atttcagcaa tatatatata tatttcaagg atataccatt ctaatgtctg
    12001 cccctatgtc tgcccctaag aagatcgtcg ttttgccagg tgaccacgtt ggtcaagaaa
    12061 tcacagccga agccattaag gttcttaaag ctatttctga tgttcgttcc aatgtcaagt
    12121 tcgatttcga aaatcattta attggtggtg ctgctatcga tgctacaggt gtcccacttc
    12181 cagatgaggc gctggaagcc tccaagaagg ttgatgccgt tttgttaggt gctgtggctg
    12241 gtcctaaatg gggtaccggt agtgttagac ctgaacaagg tttactaaaa atccgtaaag
    12301 aacttcaatt gtacgccaac ttaagaccat gtaactttgc atccgactct cttttagact
    12361 tatctccaat caagccacaa tttgctaaag gtactgactt cgttgttgtc agagaattag
    12421 tgggaggtat ttactttggt aagagaaagg aagacgatgg tgatggtgtc gcttgggata
    12481 gtgaacaata caccgttcca gaagtgcaaa gaatcacaag aatggccgct ttcatggccc
    12541 tacaacatga gccaccattg cctatttggt ccttggataa agctaatctt ttggcctctt
    12601 caagattatg gagaaaaact gtggaggaaa ccatcaagaa cgaattccct acattgaagg
    12661 ttcaacatca attgattgat tctgccgcca tgatcctagt taagaaccca acccacctaa
    12721 atggtattat aatcaccagc aacatgtttg gtgatatcat ctccgatgaa gcctccgtta
    12781 tcccaggttc cttgggtttg ttgccatctg cgtccttggc ctctttgcca gacaagaaca
    12841 ccgcatttgg tttgtacgaa ccatgccacg gttctgctcc agatttgcca aagaataagg
    12901 ttgaccctat cgccactatc ttgtctgctg caatgatgtt gaaattgtca ttgaacttgc
    12961 ctgaagaagg taaggccatt gaagatgcag ttaaaaaggt tttggatgca ggtatcagaa
    13021 ctggtgattt aggtggttcc aacagtacca ccgaagtcgg tgatgctgtc gccgaagaag
    13081 ttaagaaaat ccttgcttaa aaagattctc tttttttatg atatttgtac ataaacttta
    13141 taaatgaaat tcataataga aacgacacga aattacaaaa tggaatatgt tcatagggta
    13201 gacgaaacta tatacgcaat ctacatacat ttatcaagaa ggagaaaaag gaggatagta
    13261 aaggaataca ggtaagcaaa ttgatactaa tggctcaacg aaatgacggt atcgataagc
    13321 ttgatatcga attcctgcag cccgggggat ccactagttc tagagttaat taa
//
