LOCUS       sequence-208808-       10039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses pentamerised full-length protein ectodomain with no 
            N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 
            d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10039)
  AUTHORS   Knockel J, Dundas K, Yang ASP, Galaway F, Metcalf T, Gemert GV, 
            Sauerwein RW, Rayner JC, Billker O, Wright GJ
  TITLE     Systematic Identification of Plasmodium Falciparum Sporozoite 
            Membrane Protein Interactions Reveals an Essential Role for the p24 
            Complex in Host Infection.
  JOURNAL   Mol Cell Proteomics. 2021;20:100038. doi: 10.1074/mcp.RA120.002432. 
            Epub 2021 Jan 27.
  PUBMED    33515807
REFERENCE   2  (bases 1 to 10039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2021"; volume: "20:100038. doi"; pages: " 
            10.1074/mcp.RA120.002432. Epub 2021 Jan 27"
COMMENT     Sequence Label: GRP94-COMP-blac-flag-his
FEATURES             Location/Qualifiers
     source          1..10039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..329
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      352..361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3571..4356
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             4363..4428
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4435..4452
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4490..4545
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4544..4563)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6781..6799)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6867..6971
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6972..7040,7041..7832)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8003..8591
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8492..8511
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8745..8762
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8879..8900
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8915..8920,8921..8938,8939..8945)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8953..8969
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8958..8980
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8977..8993
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8977..8993
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        9264..9643
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9644..9847
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9798..9818
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9844..9868
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccgat
      421 agcagcgtgg aaggcgataa gggccccagc gacgacgtgt ccgacagcag cggcgagaag
      481 aaagaagtga agcgcgaccg ggacaccctg gaagagatcg aggaaggcga gaagcccacc
      541 gagagcatgg aaagccacca gtaccagacc gaagtgaccc ggctgatgga catcatcgtg
      601 aacagcctgt atacccagaa agaggtgttc ctgagagagc tgatctccaa cgccgccgac
      661 gccctggaaa agattcggtt cctgagcctg agcgacgaga gcgtgctggg cgaggaaaag
      721 aagctggaaa tccggatcag cgccaacaaa gagaagaaca tcctgagcat caccgacacc
      781 ggcatcggca tgaccaaggt ggacctgatc aacaacctgg gcacaatcgc caagagcggc
      841 accagcaact ttctggaagc catcagcaag tctggcggcg acatgagcct gatcggccag
      901 ttcggcgtgg gcttttacag cgccttcctg gtggccgaca aagtgatcgt gtacaccaag
      961 aacaacgacg acgagcagta catctgggag agcaccgccg atgccaagtt caccatctac
     1021 aaggacccca gaggcgccac cctgaagcgg ggcaccagaa tcagcctgca cctgaaagag
     1081 gacgccacca acctgctgaa cgacaagaaa ctgatggatc tcatctccaa gtacagccag
     1141 ttcatccagt tccctatcta cctgctgcac gagaatgtgt acaccgagga agtgctggcc
     1201 gatatcgcca aggacatggt caacgacccc aactacgaca gcgtgaaggt ggaagagaca
     1261 gacgacccta acaagaaaac ccggaccgtg gaaaagaaag tgaagaaatg gaccctgatg
     1321 aacgagcagc ggcccatctg gctgcggagc cccaaagagc tgaaggacga ggactacaag
     1381 cagttcttca gcgtgctgag cggctacaac gaccagcccc tgtaccacat ccactttttc
     1441 gccgagggcg agatcgagtt caagtgcctg atctacatcc ccagcaaggc ccctagcatg
     1501 aacgatcagc tgtactccaa gcagaactcc ctgaagctgt acgtgcggcg ggtgctggtg
     1561 gctgacgagt tcgtggaatt cctgccccgg tacatgagct tcgtgaaggg cgtggtggac
     1621 agcgacgatc tgcccctgaa cgtggccaga gagcagctgc agcagaacaa gatcctgaag
     1681 gccgtgtcca agcggatcgt gcggaagatc ctggacacct tccacaagct gtacaaagag
     1741 ggcaagaaga acaaagaaac cctgcggagc gagctggaaa acgaggccga cgaggaaaaa
     1801 aagaaagaga tcaccaagaa gctgagcgag cccagcacct acaagctcat ctacaaagag
     1861 taccggaagt tcctgaagtc cggctgctac gaggacgaca tcaacagaaa caagatcgcc
     1921 aagctgctgc tgttcaagac catgcagtac cccaagagca tcagcctgga tacctacatc
     1981 gagcacatga agcccgacca gaagttcatc tactacgcca gcggcgacag ctacgagtac
     2041 ctggccaaga tccctcagct gcagatcttc aagaagaaga atatcgacgt cctgttcctg
     2101 accgagtccg tggacgagag ctgcatccag cgggtgcagg aatacgaggg aaagaagttc
     2161 aagtccatcc agaagggcga aatcagcttc gagctgacag aggaagagaa aaaaaaggaa
     2221 cagcagatgc agaagatgta caaggccctg atcgacgtga tctccgatac cctgaagaac
     2281 cagatcttta aggtggaaat cagcagacgg ctggtggacg ccccttgcgc cgtggtgtct
     2341 acagagtggg gcctgtccgg ccagatggaa aagctgatga agatgaatgt ggccaacagc
     2401 gaccagatca aggccatgag cggccagaag attctggaaa tcaaccccaa ccaccccatc
     2461 atgatcgatc tgctgaagcg gagcgtgacc aaccccaagg acctggaact gaccaacagc
     2521 atcaagatca tgtaccagag cgccaaactg gccagcggct tcgacctgga agataccgcc
     2581 gacctggccc agattgtgta cgaccacatt aaccagaaac tgggcgtgga caacaacctc
     2641 aagatcgacg acctggaccc cagcatcttc gagacaaaga agattgagga cgagaacgac
     2701 gccagcaagt tcgaggaaga aatcaacatt gacgacgaga tccagaagaa ggataacaac
     2761 gtcgacaacg aggccaatga caagtccgac gaactcggcg cgccgtcgac ctccatcacg
     2821 gcctataaga gtgaggggga gtcagcggag ttctccttcc cactcaacct tggagaggaa
     2881 agcctgcagg gagagttgag atggaaggca gagaaggctc cttcttccca gtcctggatc
     2941 accttctccc taaagaacca aaaggtgtct gtgcagaagt ctactagcaa ccccaagttc
     3001 cagctgtccg aaacgctccc actcaccctt cagatacccc aggtctccct tcagtttgct
     3061 ggttctggca acctgaccct gactctggac agagggatac tgtatcagga agtgaacctg
     3121 gtggtgatga aagtgactca gcccgacagc aacactttga cctgtgaggt gatgggaccc
     3181 acctcaccca agatgagact gatcttgaag caggagaatc aggaggccag ggtctccagg
     3241 caggagaaag tgattcaagt gcaggcccct gaagcagggg tgtggcaatg tctactgagt
     3301 gaaggtgaag aggtcaagat ggactccaag atccaggttt tatccaaagg gttgaattcc
     3361 ggcggcggct ccggaggcgg taccggtggc ggcgacctag ccccacagat gcttcgagaa
     3421 ctccaggaga ctaatgcggc gctgcaagac gtgagagagc tcttgcgaca gcaggtcaag
     3481 gagatcacct tcctgaagaa tacggtgatg gaatgtgacg cctgcggtct agacagaaac
     3541 ctgcctccac tagcacctct gggcccacac ccagaaacgc tggtgaaagt aaaagatgct
     3601 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3661 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     3721 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     3781 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     3841 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3901 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     3961 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     4021 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     4081 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     4141 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     4201 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     4261 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4321 atcgctgaga taggtgcctc actgattaag cattggggcc ctgactacaa agaccatgac
     4381 ggtgattata aagatcatga catcgattac aaggatgacg atgacaaggg ccctcaccac
     4441 caccatcacc actaattaat taaggatccc ccgacctcga cctctggcta ataaaggaaa
     4501 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
     4561 gggagggcaa atcatttggt cgagatccct cggagatctc tagctagagg atcgatcccc
     4621 gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat
     4681 gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac atgtgacacg
     4741 gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat aaatggatgt
     4801 gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt ctgtggactg
     4861 caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca atgctggggg
     4921 acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt caatcagagg
     4981 ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag tgtttataag
     5041 gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc
     5101 agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag
     5161 ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct gtcacggttt
     5221 tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac aagggcagtg
     5281 gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt cttcattctc
     5341 cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat gtgaggtgct
     5401 cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg ttcagtggtg
     5461 gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa atactagtgt
     5521 aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg gacaagccgt
     5581 aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata atcctagtgc
     5641 aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag gtgaaccatg
     5701 ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca gcggactcca
     5761 ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg ggcccataaa
     5821 caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc gtcagccccc
     5881 acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg ctgattgtaa
     5941 ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc accccgggga
     6001 atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg atctggagga
     6061 caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct catattcacg
     6121 aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta cccaaatatc
     6181 tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
     6241 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
     6301 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     6361 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct aatagagatt
     6421 agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca aatatctgga
     6481 tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     6541 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     6601 tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg
     6661 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg
     6721 tagcatatgc tatcctcatg ataagctgtc aaacatgaga attttcttga agacgaaagg
     6781 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     6841 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6901 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6961 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7021 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7081 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7141 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7201 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7261 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7321 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7381 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7441 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7501 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7561 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7621 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7681 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7741 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7801 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7861 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7921 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7981 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8041 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8101 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8161 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8221 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8281 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8341 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8401 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8461 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8521 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8581 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8641 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8701 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8761 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     8821 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     8881 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     8941 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     9001 acgccaagct ctagctagag gtcgaccaat tctcatgttt gacagcttat catcgcagat
     9061 ccgggcaacg ttgttgccat tgctgcaggc gcagaactgg taggtatgga agatctatac
     9121 attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat aaatcaatat
     9181 tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc
     9241 atgtccaata tgaccgccat gttgacattg attattgact agttattaat agtaatcaat
     9301 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     9361 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     9421 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     9481 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt
     9541 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc
     9601 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     9661 gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     9721 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     9781 taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     9841 cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg tctgcgaggg
     9901 ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt actcttggat
     9961 cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc gagtccgcat
    10021 cgaccggatc ggaaaacct
//
